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Citations for Takara Bio :
1301 - 1350 of 2391 citations for PCR Tube since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: Isolated RNA from OSF and control tissues was immediately subjected to realtime RT PCR (using Takara PrimescriptTM one step realtime RT PCR kit ...
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bioRxiv - Genetics 2020Quote: ... 356-600bp regions encompassing the SNP of interest were amplified using CloneAmp HiFi PCR Premix (Clontech), and then cloned into the pGL4.23-mini/P vector including a minimal SV40 promoter upstream of firefly luciferase ...
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bioRxiv - Cell Biology 2020Quote: ... gDNA of CFU colonies was extracted by suspending the cell in 1×PCR buffer (Takara, R050A) containing 0.2mg/ml proteinase K (Tiangen ...
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bioRxiv - Biochemistry 2021Quote: ... qRT-PCR analysis was performed using cDNA synthesized by the Prime Script RT Reagent Kit (TaKaRa). Each qRT-PCR was conducted in a 20-μL reaction mixture containing 2 μL of diluted template cDNA ...
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bioRxiv - Cell Biology 2022Quote: ... TurboGFP was exchanged for eGFP by amplifying the eGFP sequence by PCR from peGFP-N1 (Clontech) using the following primers to introduce AgeI and NotI restriction sites ...
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bioRxiv - Cell Biology 2021Quote: ... the full-length Borr open reading frame was amplified from LD36125 by PCR using PrimeStar (Takara) and cloned between AscI and NotI sites of pENTR (ThermoFisher ...
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bioRxiv - Physiology 2021Quote: ... Second strand synthesis and PCR amplification was done by adding the Advantage 2 Polymerase Mix (Clontech) and the SINGV6 primer (10 pmol ...
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bioRxiv - Plant Biology 2022Quote: DNA was amplified from cDNA by PCR using PrimeStar GXL DNA polymerase (Takara Bio, Shiga, Japan) and verified by DNA sequencing using a 3130 Genetic Analyzer (Applied Biosystems ...
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bioRxiv - Immunology 2020Quote: ... Quantitative PCR was performed using TB Green® Premix Ex Taq™ II (Takara, Tokyo, Japan). The siRNA was purchased from Dharmacon and transfected into Raw and PM cells using the DharmaFECT 1 siRNA Transfection Reagent.
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bioRxiv - Immunology 2021Quote: ... Quantitative real-time polymerase chain reaction (PCR) was performed using TB Green Premix Taq II (TaKaRa) and a StepOnePlus Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Evolutionary Biology 2021Quote: Amplified with V4 16S rRNA primers as above using EmeraldAmp GT PCR Master Mix (TAKARA BIO).
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bioRxiv - Microbiology 2020Quote: The cDNA was purified using a NucleoSpin Gel and PCR Clean-up kit (Takara Bio, Inc.). PCR was performed with the cDNA ...
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bioRxiv - Immunology 2021Quote: ... AAV6 vector genomes were titrated by ITR-specific quantitative PCR (Takara AAVpro Titration Kit Ver.2) per the manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: ... EqPD-1 and EqPD-L1 cDNAs were amplified by PCR using TaKaRa Ex Taq (Takara Bio) and specific primers (Supplementary Table 1) ...
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bioRxiv - Immunology 2021Quote: ... which involves the following steps: i) cDNA amplification (Terra PCR direct Polymerase, Takara, cat. no. 639270), ii ...
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bioRxiv - Immunology 2021Quote: ... using the modified (Switching Mechanism At 5’ End of RNA Transcript) PCR cDNA synthesis protocol (Clontech) and oligonucleotides as described below (Table S3) ...
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bioRxiv - Genomics 2021Quote: ... according to the instructions of SYBR®Green Real-time PCR Master Mix (Takara, Dalian, China). Reaction conditions were 95°C for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR was performed with Advantage-HD DNA polymerase (Takara Bio, Saint-Germain-en-Laye, France) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... and quantitative PCR (qPCR) was performed to detect EGFR levels by using SYBR-Green dye (Takara). Further ...
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bioRxiv - Genetics 2020Quote: ... Sequencing libraries were made of the 4C PCR products using Thruplex DNA-seq kit (Takara Bio). 4C libraries were subjected to Agencourt AMPure XP Bead cleanup (Beckman Coulter ...
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bioRxiv - Neuroscience 2021Quote: ... rat Txn1 cDNAs were amplified by PCR using LA-Taq polymerase (Takara Bio Inc, Shiga, Japan) with FW-primer ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCR procedure followed the instructions for Tks Gflex DNA polymerase (TaKaRa Bio Inc., Shiga, Japan). The amplified PCR fragments were subcloned into the pGEM T Vector system (Promega Corporation ...
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bioRxiv - Immunology 2022Quote: ... Real-time PCR analysis was performed using the Probe qPCR Mix (Takara Bio, Otsu, Shiga, Japan) and Applied Biosystems 7500 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: ... was subcloned by PCR into Xho1/BamH1 restriction sites of lentiviral expression vector pLVX-puro (Clontech), using 5’ region primer ...
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bioRxiv - Cancer Biology 2022Quote: ... was subcloned by PCR into Xho1/BamH1 restriction sites of lentiviral expression vector pLVX-puro (Clontech), using 5’ region primer ...
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bioRxiv - Cell Biology 2022Quote: ... GFP-Eps8-ΔCAP and IRSp53-mCherry were PCR amplified using PrimeSTAR GXL DNA Polymerase (Takara Bio) and the amplicons were extracted from a 1% agarose gel (Monarch Gel Extraction Kit ...
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bioRxiv - Cancer Biology 2022Quote: ... was subcloned by PCR into Xho1/BamH1 restriction sites of lentiviral expression vector pLVX-puro (Clontech), using 5’ region primer ...
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bioRxiv - Genomics 2022Quote: ... Digested samples were column purified using the NucleoSpin Gel and PCR Clean-Up kit (TaKaRa 740609) according to the manufacturer’s directions ...
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bioRxiv - Genetics 2022Quote: ... Reverse transcription for quantitative PCR (qPCR) analyses was performed with PrimeScript RT Master Mix kit (Takara). qPCR was performed with TransStart Top Green qPCR SuperMix kit (TransGen).
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bioRxiv - Microbiology 2022Quote: ... The cDNA was purified using a NucleoSpin Gel and PCR Clean-up kit (Takara Bio, Inc.). PCR was performed with the cDNA ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products were further introduced into the XmnI and KpnI digested pB7FWG2.0 with In-Fusion (Clontech). For dual-luciferase analysis ...
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bioRxiv - Microbiology 2022Quote: The threshold cycle (Ct) values were determined by the SmartChip Real-Time PCR software (Takara Bio). Standard curves were generated by plotting the Ct values vs ...
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bioRxiv - Plant Biology 2022Quote: ... Target DNA was amplified by PCR using TaKaRa Ex Taq DNA Polymerase (TaKaRa Bio, Tokyo, Japan). DNA segments were purified using MonoFas DNA Purification Kit I (ANIMOS ...
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bioRxiv - Neuroscience 2022Quote: ... Target sequences were amplified by 18–30 cycles of PCR using Ex-Taq™ (Takara Bio). For semiquantitative PCR ...
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bioRxiv - Molecular Biology 2022Quote: We amplified Complementary DNAs (cDNAs) of genes by PCR using PrimeSTAR Max DNA polymerase (TAKARA Bio), and cloned the PCR products into overexpression vectors EGFP-N1 (Addgene ...
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bioRxiv - Neuroscience 2023Quote: ... Physical and functional titers were obtained using the Lenti-X qRT-PCR Titration Kit (Takara 631235) and qPCR of genomic DNA following HEK293T transduction91 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: PCR reactions were performed using PrimeSTAR® Max DNA Polymerase (TAKARA Bio Inc., San Jose, CA) according to manufacturer protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... gel purified using the Nucleospin Gel and PCR Clean-Up Kit (TaKaRa Bio Inc., Kusatsu, Japan), and then cloned into the XbaI site of pHIV7/PGK-neo using InFusion cloning (TaKaRa Bio Inc. ...
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bioRxiv - Immunology 2022Quote: ... and treated with Exonuclease I (New EnglandBiolabs) before amplification by the Advantage 2 PCR kit (Clontech) and the SINGV6 primer (95°C for 1 min ...
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bioRxiv - Neuroscience 2023Quote: ... 5 μl of the PCR product was treated with 2 μl cloning enhancer (Takara-bio Inc.). The cloning reaction was prepared by adding 2 μl of 5X In-Fusion HD Cloning enzyme mix ...
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bioRxiv - Cell Biology 2023Quote: ... Lentiviral titration was performed by RT-qPCR using the Lenti-X qRT-PCR Titration Kit (Takara) and the concentration of lentivirus used for this paper was found to be above 1 × 107 copies / mL ...
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bioRxiv - Cancer Biology 2023Quote: ... a region spanning the ENE domain was amplified by conventional PCR (PrimeStar HS mix, Takara Bio) with primer sequences listed in Table S9 ...
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bioRxiv - Genetics 2023Quote: ... Reverse transcription and quantitative real-time PCR were performed with PrimeScriptTM RT Master Mix (Takara, RR037A) and TB Green Premix Ex TaqTM II (Takara ...
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bioRxiv - Molecular Biology 2023Quote: ... qRT-PCR was performed using the Thermal Cycler Dice Real Time System (Takara Bio, Kusatsu, Japan). Primers and templates were mixed with TB Green Premix Ex Taq II (Takara Bio) ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR was performed in Thermal Cycler Dice Real Time System Lite (Takara Bio) using KOD SYBR qPCR Mix (Toyobo ...
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bioRxiv - Plant Biology 2023Quote: ... The full-length CAMTA3 cDNA was PCR amplified using the HS Primestar DNA polymerase (Takara, USA) with forward and reverse primers bearing BamHI and XhoI ...
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bioRxiv - Genomics 2023Quote: ... Amplified oligonucleotides were purified using the NucleoSpin PCR clean-up and gel extraction kit (Takara Bio). CROP-seq-opti was linearized via digestion with BsmBI and alkaline phosphatase (NEB ...
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bioRxiv - Genomics 2023Quote: ... while the out/out PCRs were carried out with PrimeSTAR GXL DNA Polymerase (Takara Bio, R051A). Seamless HDR was confirmed by sequencing the junctions between the insert and the mouse genome ...
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bioRxiv - Cell Biology 2023Quote: ... Real-time qPCR amplification was performed using the PrimeScript™ RT-PCR Kit (Takara, Catalog#RR420). Alp ...
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bioRxiv - Immunology 2023Quote: ... qRT-PCR was performed using the SYBR® Premix Ex Taq™ II (Takara, Shiga, Japan) Kit and a StepOnePlus Real-Time PCR instrument ...