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Citations for Takara Bio :
1251 - 1300 of 2391 citations for PCR Tube since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The digested PCR product was cloned into a TA-vector by Mighty TA-cloning Kit (Takara). After LacZ selection ...
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bioRxiv - Genomics 2022Quote: ... the cDNAs were amplified with an appropriate number of PCR cycles using LA Taq polymerase (Takara), and then the reactions were treated with 200 U/mL of Exonuclease I (NEB ...
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bioRxiv - Genomics 2020Quote: ... 500-1000ng of total RNA was reverse transcribed using the Advantage RT-for-PCR Kit (Takara) and qPCR performed in quadruplet using SYBR Green PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Real-time PCR reactions were performed using TB Green Premix Ex Taq reagent (TaKaRa, Cat#RR420A) on QuantStudio 7 Flex (Applied Biosystems) ...
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bioRxiv - Biochemistry 2019Quote: ... Real-time PCR was performed using SYBR Premix Ex Taq™ II (Tli RNaseH Plus, TaKaRa). GAPDH mRNA levels were used for normalization ...
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bioRxiv - Molecular Biology 2019Quote: ... GFI1B-BirA*:HA forms were expressed from PCR amplified sequences subcloned into pLVX-Tight-Puro (Clontech) with Not1/Mlu1 for doxycycline inducible expression with the Tet-On Advanced (Clontech ...
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bioRxiv - Developmental Biology 2019Quote: ... Quantitative real-time RT–PCR was carried out using the SYBR premix Ex Taq kit (TaKaRa). Briefly ...
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bioRxiv - Genomics 2020Quote: ... Each sample was PCR amplified for 9 cycles using HS LA Taq (Takara, Mountain View, CA) and cleaned with 0.7X AMPure beads to remove small fragments and excess reagents (Beckman Coulter ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative real-time PCR was conducted using SYBR Premix Ex Taq (Takara Bio Inc., Shiga, Japan) and a Thermal Cycler Dice Real Time System TP800 (Takara Bio Inc.) ...
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bioRxiv - Plant Biology 2020Quote: Real-time PCR was performed using TB Green Premix Ex Taq™ II (Takara, Dalian, China). An ABI 7500 real-time PCR system (Applied Biosystems ...
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bioRxiv - Biochemistry 2020Quote: ... Human IFITM3 cDNA was purchased from Open Biosystems and PCR cloned into pCMV-HA vector (Clontech). All mutants of IFITM3 were generated by using QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... All modification for plasmids were performed using overlap PCR mutagenesis with InFusion cloning (Takara Biosciences, USA). Viral genome and support plasmids were maintained in chemically-competent Stbl2 E ...
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bioRxiv - Microbiology 2020Quote: ... Realtime PCR was performed using TB Green® Advantage® qPCR premix (Takara Bio USA, Inc.) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative RT-PCR was performed with SYBR® Premix Ex Taq™ (Takara Bio, Shiga, Japan) in a 7300 Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... Semi-quantitative PCR was conducted using LA taq with GC buffer I (Takara medical co. ltd) using the methods in manual.
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bioRxiv - Microbiology 2021Quote: ... specific primers were designed and PCR reactions were performed using SYBR Green Premix Ex Taq (Takara), according to the manufacturer’s protocol and the primers listed in Table S4 ...
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bioRxiv - Microbiology 2020Quote: ... the extracted DNA was subjected to PCR using Takara Ex Taq Hot Start Version (Takara Bio) with the following conditions ...
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bioRxiv - Molecular Biology 2021Quote: ... and assembled with PCR-amplified homology arms using In-Fusion® HD Cloning Kit (Takara 638920). Final products were verified by enzymatic digestion and sequencing ...
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bioRxiv - Microbiology 2020Quote: ... The PCR-amplified elements were ligated in a single reaction step by In-Fusion cloning (Clontech) upstream of the HA3 tag in XmaI and AfeI sites of pLN-HA3-loxP ...
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bioRxiv - Cell Biology 2021Quote: ... PCR amplification were conducted using Ex Taq in accordance with the manufacturer’s instructions (TaKaRa Bio, Inc.).
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bioRxiv - Cell Biology 2021Quote: ... The real-time quantitative PCR analysis was performed using SYBR Premix Ex Taq II (TAKARA, China) on LightCycler 480 PCR System (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... EBIV M/L segment-specific sequences were amplified by PCR including Universal Primer A Mix (Takara), Gene-Specific Primers (GSPs ...
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bioRxiv - Physiology 2022Quote: ... PCR reaction was conducted using SYBR qPCR Premix Ex Taq II Tli RNase H+ (TAKRR820W, Takara). Primer pairs are listed in supplementary table S2.
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bioRxiv - Microbiology 2022Quote: ... The PCR product and pET28b plasmid were digested with restriction endonuclease EcoRI and HindIII (Japanese Takara), then ligated with T4 DNA Ligase (Japanese Takara) ...
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bioRxiv - Microbiology 2022Quote: ... Amplifying PCR of the first stand cDNA product was then performed using SeqAmp DNA Polymerase (Takara) with a nested 3’ primer to the constant genes and a 5’ universal primer based on universal primer sites added to the 5’ end during cDNA generation ...
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bioRxiv - Microbiology 2022Quote: ... GISAID ID: EPI_ISL_408667)29 were prepared by PCR using PrimeSTAR GXL DNA polymerase (Takara, Cat# R050A). A linker fragment encoding hepatitis delta virus ribozyme ...
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bioRxiv - Microbiology 2022Quote: ... Samples were analyzed using a One-Step SYBR PrimerScript reverse transcription (RT)-PCR kit (TaKaRa, Japan) on Applied Biosystems QuantStudio ...
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bioRxiv - Genetics 2022Quote: ... Each 30 µL PCR reaction volume contained 15 µL Premix Ex Taq enzyme (Takara Biomedical Technology), 0.2 µL BSA ...
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bioRxiv - Genetics 2022Quote: ... The 20 µL PCR reaction volume included 10 µL Premix Ex Taq enzyme (Takara Biomedical Technology), 0.2 µL BSA ...
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bioRxiv - Genetics 2022Quote: ... and then used to synthesize cDNA with TaKaRa RNA PCR™ Kit (AMV) Ver.3.0 (TaKaRa). Quantitative real-time PCR (qPCR ...
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bioRxiv - Cancer Biology 2022Quote: Genotyping was done using the Terra PCR Direct Polymerase Mix and kits (Takara Bio; Shiga, Japan). The primers used for genotyping are given in supplementary table 1 ...
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bioRxiv - Cell Biology 2022Quote: ... and each reaction contained 1 μl of cDNA and the SYBR Green PCR mix (Takara, RR420A), to which gene-specific forward and reverse PCR primers were added ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Samples were analyzed using a One-Step SYBR PrimerScript reverse transcription (RT)-PCR kit (TaKaRa, Japan) on an Applied Biosystems QuantStudio ...
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bioRxiv - Developmental Biology 2021Quote: ... PrimeScript RT-PCR and SYBR Premix ExTaq Kits were from Takara (Cat#2641A, Cat#RR420, Japan). BCA protein assay and ECL kits were from Beyotime Institute of Biotechnology (Shanghai ...
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bioRxiv - Microbiology 2020Quote: ... were amplified by PCR using genome as template and cloned into T-Vector pMD19 (Simple, Takara). Primers containing the mutations were used to PCR the whole derived pMD19 plasmid constructed above ...
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bioRxiv - Immunology 2020Quote: ... real-time PCR analysis was carried out using Premix Ex Taq (probe qPCR) (Takara, Dalian, China). Real-time PCR was performed with WSSV-specific primers WSSV-RT1 (5’-TTGGTTTCATGCCCGAGA TT-3’ ...
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bioRxiv - Microbiology 2021Quote: ... GISAID ID: EPI_ISL_408667)37 were prepared by PCR using PrimeSTAR GXL DNA polymerase (Takara, cat# R050A). A linker fragment encoding hepatitis delta virus ribozyme ...
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bioRxiv - Genomics 2022Quote: ... and post-capture PCR amplification was performed for 16-18 cycles using PrimeSTAR GXL Polymerase (Takara) at an annealing temperature of 60°C.
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bioRxiv - Synthetic Biology 2019Quote: ... Real-time PCR was performed using SYBR Premix Ex Taq™ II (Tli RNaseH Plus, TaKaRa). GAPDH mRNA levels were used for normalization ...
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bioRxiv - Molecular Biology 2020Quote: ... The qRT-PCR analyses were performed using SYBR Premix ExTaq TM II Mix (TaKaRa, Shiga, Japan). Actin3 (GenBank accession no. ...
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bioRxiv - Genetics 2019Quote: Regions orthologous to human placental igDMR were PCR-amplified with TaKaRa EX Taq HS (TaKaRa Bio) with primers specific for chimpanzee and rhesus macaque CGIs (Supplementary Table 4) ...
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bioRxiv - Genomics 2019Quote: ... and the expression of IFN-β and IL-6 were measured by quantitative PCR (TAKARA, RR820A). The sequences of the primers were listed in the Supplementary Table 4.
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bioRxiv - Microbiology 2019Quote: ... cDNA was synthesized with 100 ng RNA using Advantage RT-PCR Kit (Clontech, Mountain View, USA). Reaction condition for real time PCR was 95°C for 3min (1 cycle) ...
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bioRxiv - Physiology 2019Quote: ... PCRs for each sample were performed as triplicates with the SYBR Green dye (TaKaRa, Dalian, China) and StepOnePlus™ Real-Time PCR System according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... The qPCR was performed with the MIR-X miRNA qRT-PCR TB Green Kit from TAKARA. To calculate the relative fold of expression of mir-243-3p between generations and genotypes we used delta-delta Ct calculations according to the Reference Unit Mass method (65) ...
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bioRxiv - Cell Biology 2019Quote: ... qRT-PCR was carried out using TB Green™ Premix Ex Taq™ II (Takara, RR820A) and Roche Light Cycler 96 system (Roche) ...
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bioRxiv - Plant Biology 2019Quote: ... Quantitative real time polymerase chain reaction (qRT-PCR) was performed using SYBR Premix ExTaq (Takara, Japan) in a TP800 Real-Time PCR machine (Takara ...
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bioRxiv - Neuroscience 2020Quote: ... PCR amplified and inserted into the DsRed-Express2 plasmid using in-fusion cloning (Takara Bio, CA). St3-Halo was generated by replacing the GFP in St3-eGFP with Halo tag (GenScript USA Inc ...
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bioRxiv - Plant Biology 2019Quote: ... PCR fragments were gel-purified and ligated into the pMD 19-T Vector (TaKaRa, Dalian, China), and confirmed by sequencing from both strands.
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bioRxiv - Plant Biology 2019Quote: ... fragments were amplified by PCR and ligated using the In-Fusion HD Cloning Kit (Takara Bio). Using RTS 100 Wheat Germ CECF Kit (Roche) ...