Labshake search
Citations for Takara Bio :
9701 - 9750 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... Lenti-X 293T cells (Takara 632180) are a subclone of HEK293T which supports high levels of viral protein expression ...
-
bioRxiv - Neuroscience 2022Quote: ... as confirmed by PCR were treated with Cre recombinase gesicles (Takara Bio) with 6 µg/mL Polybrene (Sigma ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... and then reversely transcribed to synthesize the first strand of cDNA using SmartScribe Rtase (Clontech, Mountain View, CA, USA). Owing to the terminal transferase activity ...
-
bioRxiv - Neuroscience 2022Quote: ... These guides were individually cloned into pAAV-U6-sasgRNA-CMV-mCherry-WPREpA at the BstXI and XhoI restriction enzyme sites using the In-Fusion HD cloning kit (Clontech). rAAV vectors were generated using similar plasmids and cloning methods as was referenced in (Matharu et al. ...
-
bioRxiv - Neuroscience 2022Quote: ... The reverse transcription products were amplified by the PrimeSTAR DNA Polymerase (TaKaRa) using Exon9 specific primers ...
-
bioRxiv - Neuroscience 2022Quote: ... The reverse transcription products (cDNA) were amplified using PrimeSTAR DNA polymerase (TaKaRa, Shiga, Japan) and specific primers ...
-
bioRxiv - Molecular Biology 2022Quote: ... qPCR was performed using the SYBR Premix Ex Taq II Kit (TaKaRa Biotechnology, Dalian, Liaoning, China) on ABI 7500 Sequence Detection System software version 1.2.3 (Applied Biosystems ...
-
bioRxiv - Molecular Biology 2022Quote: ... the total RNA was reverse transcribed to cDNA using the Prime ScriptTM RT reagent kit (TaKaRa Biotechnology). qPCR was performed using the SYBR Premix Ex Taq II Kit (TaKaRa Biotechnology ...
-
bioRxiv - Molecular Biology 2022Quote: ... Lentiviral supernatant was produced as mentioned earlier and concentrated using Lenti-X concentrator (Takara) followed by storage at -80°C in aliquots.
-
bioRxiv - Molecular Biology 2022Quote: ... Lysozyme solution and Protease Inhibitor Cocktail (Takara Bio USA, Inc., CA). The suspension was sonicated 10 sec for 3 times with a 30 s pause ...
-
bioRxiv - Molecular Biology 2022Quote: ... were cloned into pGBKT7 DNA-BD (Takara Bio), pGADT7-AD (Takara Bio) ...
-
bioRxiv - Molecular Biology 2022Quote: Yeast two-hybrid screening was performed using the Matchmaker Gold Yeast Two-Hybrid System (Takara Bio) as described by the manufacturer (Young ...
-
bioRxiv - Molecular Biology 2022Quote: ... vectors using restriction cloning or the In-Fusion HD Cloning Plus kit (Takara Bio) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... RNA was extracted from cells using the TRIzol reagent (Takara, Shiga, Japan) and reverse transcribed using the PrimeScript™ RT reagent Kit (Takara) ...
-
bioRxiv - Molecular Biology 2022Quote: ... 0.2 U/μL of ribonuclease inhibitor (porcine liver) (TaKaRa Bio) in 44.5 μL of the RNase-free Buffer RDD was added to each sample ...
-
bioRxiv - Molecular Biology 2022Quote: ... 0.25 U/μL AMV reverse transcriptase XL (TaKaRa Bio), and 0.125 μM of an oligo (dT ...
-
bioRxiv - Molecular Biology 2022Quote: ... Prime Script MMLV reverse transcriptase (TaKaRa Bio) and 0.125 μM of the oligo (dT ...
-
bioRxiv - Molecular Biology 2022Quote: ... Mammalian expression vector for Flag-EBNA1 contained B95-8 EBNA1 lacking the GA repeats (aa 101-324) under the control of CMV-3XFLAG promoter in a plasmid derived from pREP10 (Clontech) containing ...
-
bioRxiv - Molecular Biology 2022Quote: ... Five microliters of DNase I (15 K units, TaKaRa Bio), 0.2 U/μL of ribonuclease inhibitor (porcine liver ...
-
bioRxiv - Molecular Biology 2022Quote: Total RNA was isolated from ligament fibroblasts using RNAiso Plus (TaKaRa Bio. Dalian, China). Equal amounts of RNA samples (200 ng ...
-
bioRxiv - Molecular Biology 2022Quote: ... Software) (Takara, China). IDT (https://eu.idtdna.com/pages ...
-
bioRxiv - Neuroscience 2022Quote: ... AAV vectors were subsequently extracted from the pellet using an AAVpro purification Kit (Takara bio). The eluted solution was concentrated using an Amicon Ultra-15 centrifugal filter unit (Takara bio) ...
-
bioRxiv - Neuroscience 2022Quote: ... The eluted solution was concentrated using an Amicon Ultra-15 centrifugal filter unit (Takara bio). The titre was measured via real-time PCR using an AAVpro Titration Kit for Real Time PCR Ver ...
-
bioRxiv - Neuroscience 2022Quote: ... respectively (Takara Bio). The transfected cells were incubated in five 225 cm2 flasks for three days ...
-
bioRxiv - Neuroscience 2022Quote: ... 2 (Takara Bio).
-
bioRxiv - Neuroscience 2022Quote: ... then incubated overnight with the primary antibody rabbit anti-dsRed (1:1000 in PBST; Clontech, AB_10013483) at 4°C ...
-
bioRxiv - Neuroscience 2022Quote: ... Target sequences were amplified by 18–30 cycles of PCR using Ex-Taq™ (Takara Bio). For semiquantitative PCR ...
-
bioRxiv - Neuroscience 2022Quote: ... eluted RNAs were reverse-transcribed using PrimeScript™ 1st strand cDNA Synthesis Kit (Takara Bio) and subjected to PCR ...
-
bioRxiv - Neuroscience 2022Quote: ... PolyA+ mRNA was extracted from total RNA using Oligotex TM-dT30 mRNA Purification Kit (Takara Bio), according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... Pak1 fragment (amino acids 270–521) was PCR-amplified from pCMV6M-Pak1 and subcloned into pCold-Pros2 vector (Takara Bio Inc.) to generate pCold-Pros2-Pak1cat ...
-
bioRxiv - Neuroscience 2022Quote: Total RNA was isolated from 8-week-old wild-type or Inka2-/- mice forebrains using the RNAiso Plus reagents (Takara Bio). PolyA+ mRNA was extracted from total RNA using Oligotex TM-dT30 mRNA Purification Kit (Takara Bio) ...
-
bioRxiv - Neuroscience 2022Quote: cDNA fragments corresponding to Inka1 and Inka2 ORFs were isolated using RT-PCR of the RNAs of E12 embryonic and adult mice brains and subcloned in-frame into a pEGFP-C2 expression vector (Takara Bio Inc., Shiga, Japan). To construct the CAG promoter-driven Inka2 in mammalian cells ...
-
bioRxiv - Neuroscience 2022Quote: ... The following primary antibodies were used: mouse anti-Nc82 (Laissue et al., 1999) rabbit anti-DsRed (TaKaRa Bio ...
-
bioRxiv - Molecular Biology 2022Quote: ... Each sample was then incubated at 37°C for 30 min with 20 U of mRNA-Interferase™ MazF enzyme (Takara, Cat# 2415A) in a 10 μL volume reaction containing 1X MazF reaction buffer ...
-
bioRxiv - Molecular Biology 2022Quote: ... and COUP-TF2LBDΔAF2-TRF1 were generated using the InFusion cloning method (Clontech). Vectors were digested with BamHI and incubated overnight at 37 °C (5 µg Vector ...
-
bioRxiv - Molecular Biology 2022Quote: ... One microgram of total RNA was used as a template in reverse transcription reactions using 0.2 mM dNTP (TakaRa Bio), 1 U/μL ribonuclease inhibitor (porcine liver ...
-
bioRxiv - Molecular Biology 2022Quote: ... 1 U/μL ribonuclease inhibitor (porcine liver) (TaKaRa Bio), 0.25 U/μL AMV reverse transcriptase XL (TaKaRa Bio) ...
-
bioRxiv - Neuroscience 2022Quote: ... and were replaced with the FP fragment in Cyan pre-eGRASP(p32) and post-eGRASP via Infusion (Takara Bio, Shiga, Japan) to yield the Pre-Sphere-iGluSnFR(p32 ...
-
bioRxiv - Neuroscience 2022Quote: ... PCR was performed using the TB Green Ex Taq II Mix (Takara Bio) and the Thermal Cycler Dice Real-Time System (Takara Bio).
-
bioRxiv - Neuroscience 2022Quote: ... Real-time PCR was performed using TB Green Premix Ex Taq II (Takara, Japan) and ViiA7 Real-Time PCR system (Applied Biosystems ...
-
bioRxiv - Molecular Biology 2022Quote: ... The DNA band was cut out and purified using the Nucleospin® Extract II Kit (Clontech Laboratories) protocol ...
-
bioRxiv - Molecular Biology 2022Quote: ... PCR products covering the whole sesRNA region was generated with CloneAmp HiFi PCR Premix (Takara, Cat. No. 639298), and purified with NucleoSpin Gel and PCR Clean-up kit (Takara ...
-
bioRxiv - Molecular Biology 2022Quote: ... The 2× SYBR Green qPCR Mix Kit (TaKaRa, Japan) and the cDNA concentration and primers described above were used ...
-
bioRxiv - Molecular Biology 2022Quote: ... and also remaining polymerase enzyme by use of Nucleospin® Extract II Kit (Clontech Laboratories) following Manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2022Quote: ... The titer of adenovirus was examined using an Adeno-XTM Rapid Titer Kit (Takara Bio USA) and determined to be 3.4 × 1011 ifu/ml ...
-
bioRxiv - Molecular Biology 2022Quote: ... The edit locus was PCR amplified using Titanium® Taq DNA Polymerase (Takara, 639208) followed by Sanger Sequencing.
-
bioRxiv - Molecular Biology 2022Quote: ... The titer of harvested lentivirus was established using the Lenti-X RT-qPCR Titration Kit (Takara Bio, Cat. no. 631235). A representative calibration plot and LV tittering of pAIO is added as Supplementary data S4.
-
bioRxiv - Molecular Biology 2022Quote: ... 6xHN Polyclonal Antibody (1:2000, Takara, CA, USA) at 4°C for overnight ...
-
bioRxiv - Molecular Biology 2022Quote: ... red fluorescence protein (DsRed) and NK-NT or NKN1 fragments were cloned into pET6xHN-N Vector (Takara, CA, USA). HEK293T cells were cultured in FP medium (DMEM containing 10% FBS ...
-
bioRxiv - Molecular Biology 2022Quote: ... Amplicons were cloned into vector pRACE (Takara) and individual clones were selected for nucleotide sequencing.