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Citations for Takara Bio :
9601 - 9650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... using In-Fusion (Takara) cloning technology ...
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bioRxiv - Plant Biology 2022Quote: ... and UhSee1ΔSP were cloned into the pGBKT7 vector (Clontech) for expression with the GAL4 DNA-BD under control of the constitutive ADH1 promoter ...
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bioRxiv - Microbiology 2022Quote: ... coli strain BL21(DE3)-pGro7/GroEL (Takara Bio) was grown in 500 mL of ZYP medium [47] (supplemented with 100 μg/mL ampicillin ...
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bioRxiv - Genomics 2022Quote: ... 10ul of the reaction mix was transformed directly into 100ul of Stellar chemically competent cells (Takara Bio), cells were allowed to recover at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... TALON Metal Affinity Resin (Clontech); Mono Q 5/50 GL (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... restriction enzymes and DNA ligation kit ver.2 (Takara Bio); pET21a and E ...
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bioRxiv - Biochemistry 2022Quote: ... PCR was performed using a 0.25 U PrimeSTAR HS DNA polymerase (TaKaRa) with 0.2 mM dNTP mixture ...
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bioRxiv - Microbiology 2022Quote: ... RNA samples with sufficient material (10 pg–10 ng) were passed to whole-transcriptome library preparation using the SMART-Seq v4 PLUS Kit (Takara Bio) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... to encode a 3C-cleavable His6-GST-fusion using In-Fusion enzyme (Clontech, Takara), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... a DNA fragment encoding residues 1-132 was amplified using the RPA expression plasmid as a template and CloneAmp Hi-Fi PCR master mix (Clontech, Takara) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... a DNA fragment encoding residues 1-132 was amplified using the RPA expression plasmid as a template and CloneAmp Hi-Fi PCR master mix (Clontech, Takara) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... to encode a 3C-cleavable His6-GST-fusion using In-Fusion enzyme (Clontech, Takara), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: HeLa Tet-On cells (Takara Bio Inc.) were cultured at 37 °C at 5% CO2 in air atmosphere ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was applied on a Talon affinity column (10 ml; Clontech) equilibrated in buffer A ...
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bioRxiv - Bioengineering 2022Quote: ... Sections were incubated overnight at 4°C in primary antibody diluted in PBS (1:100 monoclonal mouse anti-GFP; 632380, Clontech). After washing with PBS ...
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bioRxiv - Biochemistry 2022Quote: ... the supernatant was batch bound to TALON IMAC resin (Clontech) for 2 h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Scaffolding protein was purified on a TALON Superflow metal affinity column (Takara Bio, San Jose, CA). Fractions containing scaffolding protein were pooled and concentrated in 12-14kDa MWCO dialysis tubing (Spectrum Chemical ...
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bioRxiv - Bioengineering 2022Quote: ... PCR Master Mix (TaKaRa), and 5 µL of template DNA (plasmid standard or sample DNA) ...
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bioRxiv - Immunology 2022Quote: ... Protein was purified in a PBS buffer (pH 7.4) from filtered supernatants by using either StrepTactin resin (IBA) or Cobalt-TALON resin (Takara). Affinity tags were cleaved off from eluted protein samples by HRV 3C protease ...
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bioRxiv - Developmental Biology 2022Quote: ... Libraries were prepared using the directional PrepX RNA-seq library preparation protocol on the automated Apollo 324TM NGS Library Prep System (Takara Bio, CA), with a cDNA insert average size of ∼200bp.
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bioRxiv - Cell Biology 2022Quote: ... For this 6 μg lentivirus cDNA construct expressing TMEM115-3xHA (GolgiTAG) was incubated with 3.8 μg pGAG/Pol (Clontech. Cat# 631247) and 2.2 μg pVSVG (Clontech ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were transfected with mRNAs or DNAs via liposomes (Invitrogen Lipofectamine or Takara Xfect) or PEI (Polysciences PEImax ...
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bioRxiv - Bioengineering 2022Quote: Arc DNA plasmids were transfected into the donor cell culture via PEI or Xfect transfection reagent (Takara) following manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... The capsid sequence of pRC5 (Takara Bio, Shiga, Japan) was replaced by AAV.GT5 and AAV-Spark100 for production of each AAV serotype.
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bioRxiv - Bioengineering 2022Quote: ... 800 μg/mL hygromycin (Clontech, San Jose, CA) was used for the selection ...
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bioRxiv - Bioengineering 2022Quote: ... Inc (Takara Bio, San Jose, CA). Converted cDNA products were next column purified and subsequently amplified using universal primers designed relative to an internal consensus sequence derived from analysis of 800 patient samples ...
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bioRxiv - Bioengineering 2022Quote: ... RNA to cDNA conversion was performed using the SMART cDNA Library Construction Kit by Clontech Laboratories ...
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bioRxiv - Cancer Biology 2022Quote: Sequencing libraries were prepared from RNA using the SMARTer Stranded Total RNA-Seq Kit v3 for picogram input material (Takara, #634488). Libraries were sequenced as paired-end 101 bp (tumoroid samples ...
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bioRxiv - Cancer Biology 2022Quote: ... 4×106 B cells per well were plated in 2 mL on 6-well plates that had been coated with RetroNectin (25 μg/mL, 4°C, overnight; #T100B, Takara), blocked with 2% BSA in PBS (1h ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted using Trizol (Takara Bio, Otsu, Japan). RNA (1 μg ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative PCR was alternatively performed for the cDNA using the SYBR® Premix Ex Taq™ (Tli RNase H Plus) (TAKARA-Clontech) using a QuantStudio5 system (Applied Biosystems) ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative PCR was alternatively performed for the cDNA using the SYBR® Premix Ex Taq™ (Tli RNase H Plus) (TAKARA-Clontech) using a QuantStudio5 system (Applied Biosystems) ...
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bioRxiv - Biochemistry 2022Quote: ... from which cDNA was obtained by reverse transcription using the PrimeScript RT Reagent Kit (TaKaRa, Kusatsu, Japan). Transcript levels of RvNaRL were examined using a real-time PCR system (LightCycler 96 system ...
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bioRxiv - Biochemistry 2022Quote: ... Doxycycline was from Clontech (631311). The IP-One HTRF kit was from CisBio (62IPAPEB) ...
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bioRxiv - Biochemistry 2022Quote: ... cDNAs were generated by PCR amplification in 50 µl-total reaction volume using the Advantage 2 PCR Enzyme System (Clontech, #639206). PCR reaction was performed using 20 µl cDNA from previous step ...
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bioRxiv - Biochemistry 2022Quote: ... 0.8 μL of Guide-it Recombinant Cas9 (10 μg/μL, Takara) was added to the above PCR tube with the prepared sgRNA and incubated at 37°C for 5 min to assemble the ribonucleotide protein (RNP ...
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bioRxiv - Neuroscience 2022Quote: Human embryonic stem cell (hESC)-derived cerebral organoids (hCOs) were generated from a commercially available hESC stem cell line (Takara Bio, Osaka, Japan), using the STEMdiff cerebral organoid kit (STEMCELL Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by lysis in xTractor buffer (Takara) per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysates were cleared by centrifugation and immobilized on TALON metal affinity resin (Takara) per the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Transduction of T cells was performed with Retronectin (Takara) as previously described (22 ...
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bioRxiv - Microbiology 2022Quote: ... was used as a template to generate the pCMV-myc-SFPQ ΔRRM1-2 by mutagenesis using the InFusion HD kit from Clontech (catalog number 639649 ...
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bioRxiv - Biochemistry 2022Quote: ... Lentivirus particles were concentrated with Lenti-XTM Concentrator (Takara Bio). SH-SY5Y was transduced by lentivirus before differentiation ...
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bioRxiv - Molecular Biology 2022Quote: ... the structural homologue regions from human and gecko EVX1AS were in vitro transcribed (IVT) using a T7 RNA Polymerase (Takara Bio, San Jose, CA). IVT lncRNAs were then purified ...
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bioRxiv - Immunology 2022Quote: ... NETs were treated with 2 U micrococcal nuclease (Takara Bio) for 10 min at RT ...
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bioRxiv - Cell Biology 2022Quote: ... Lenti-X HEK293T cells were transfected with either pLVX-NME6Myc-Flag or pLVX-NME6H137N Myc-Flag using Lenti-X Packaging Single Shots (Takara). The viral supernatant was collected after 48 h ...
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bioRxiv - Cell Biology 2022Quote: RNA-seq library was generated according to SMART-seq v4 Ultra low input RNA kit (Takara). In brief ...
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bioRxiv - Cell Biology 2022Quote: ... and library was constructed using Takara Pico-Input Strand-Specific Total RNA-seq for Illumina (Takara). RNA-seq was performed on an Illumina NovaSeq 6000 according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR products were subcloned into pcDNA5 FRT/TO HA FLAG Hu Jaw1 or pcDNA5 FRT/TO Hu Jaw1 (digested with HpaI/XhoI) using an In-Fusion HD Cloning Kit (#Z9648N; TaKaRa, Kusatsu, Japan), resulting in pcDNA5 FRT/TO HA FLAG Hu Jaw1 opsin ...
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bioRxiv - Cell Biology 2022Quote: ... and the PCR products were subcloned into the pcDNA3.1(+) vector (digested with KpnI/BamHI) using an In-Fusion HD Cloning Kit (#Z9648N; TaKaRa, Kusatsu, Japan), resulting in pcDNA3.1(+ ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR products were subcloned into the pcDNA5 FRT/TO HA FLAG Ms Jaw1 (digested with HpaI/SphI) by double-insert cloning using an In-Fusion HD Cloning Kit (#Z9648N; TaKaRa, Kusatsu, Japan), resulting in pcDNA5 FRT/TO HA FLAG Ms Jaw1 504–508A ...