Labshake search
Citations for Takara Bio :
9951 - 10000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2021Quote: ... three gene-specific reverse primers were designed and a nested PCR program was used according to the protocol of a genome walking kit (Takara, Japan). The sequences of the primers used for amplification are shown in Table S1 ...
-
bioRxiv - Developmental Biology 2021Quote: ... SMART-Seq v4 Ultra-Low Input RNA kits (TaKaRa Bio) were used to generated polyA selected libraries from total RNA ...
-
bioRxiv - Developmental Biology 2021Quote: ... the pellet was resuspended in 0.5ml to 1.5ml of pre-warmed differentiation medium N2B27 (Ndiff227, Takara, Y40002) via pipetting up-and-down 5-15 times with a P1000 pipette until remaining clumps of mESCs were separated into single cells.
-
bioRxiv - Developmental Biology 2021Quote: The MmAtxn10 coding sequence was cloned into the pEGFP-N1 vector (Clontech) using primers designed with XhoI and AgeI restriction sites ...
-
bioRxiv - Developmental Biology 2021Quote: ... mRNA was isolated using PrepX PolyA-8 protocol (Takara 640098). The mRNA samples were then processed for cDNA preparation using PrepX mRNA-8 (Takara 640096 ...
-
bioRxiv - Developmental Biology 2021Quote: ... The mRNA samples were then processed for cDNA preparation using PrepX mRNA-8 (Takara 640096) protocol ...
-
bioRxiv - Developmental Biology 2021Quote: ... rabbit anti- dsRed (1:500; Takara Bio), guinea pig anti-Loaf (1:400 ...
-
bioRxiv - Developmental Biology 2021Quote: TdT-mediated dUTP nick end labeling (TUNEL) staining was carried out with In Situ Apoptosis Detection Kit (MK500, Takara Bio Inc., Shiga, Japan), according to the manufacturer’s instruction ...
-
bioRxiv - Developmental Biology 2021Quote: ... RNA-seq libraries were created using the SMARTer Stranded Total RNA Sample Prep Kit (Takara Bio) and sequenced using NextSeq500/550 High Output v2.5 kit (spiked with 10% PhiX ...
-
bioRxiv - Developmental Biology 2021Quote: ... using SYBR Green PCR Master Mix (Takara, Japan). The relative expression levels of the target gene (EGFP ...
-
bioRxiv - Developmental Biology 2021Quote: ... was inserted in a Tol2 vector containing the hsp70l:xxxp2a-TFP sequence (kind gift from Prof. B. Martin, Stony Brooks University, NY) by In Fusion (Clontech) cloning ...
-
bioRxiv - Developmental Biology 2021Quote: ... and reverse-transcribed using random hexamer-primers and M-MuLV reverse transcriptase (Takara, Japan). The resulted cDNA was used to perform quantitative PCR in an Applied Biosystems Step One Plus real-time PCR system (Applied Biosystems ...
-
bioRxiv - Developmental Biology 2021Quote: ... Single embryos were collected in 2 µl RNase free water with Recombinant RNase inhibitor (TAKARA) and ruptured with an RNase free needle ...
-
bioRxiv - Developmental Biology 2021Quote: ... cDNA was synthesized from 500 ng of total RNA using PrimeScript RT Master Mix (Takara). Quantitative real-time PCR (qPCR ...
-
bioRxiv - Developmental Biology 2021Quote: TdT-mediated dUTP nick end labeling (TUNEL) staining was carried out with In Situ Apoptosis Detection Kit (MK500, Takara Bio Inc., Shiga, Japan), according to the manufacturer’s instruction ...
-
bioRxiv - Developmental Biology 2021Quote: ... 2015) with MluI and inserting PCR-amplified EGFP from pEGFP-C1 (Clontech Laboratories, Mountain View, CA, USA). Tol2-mCherry was produced by digesting Tol2-EGFP-C1 with NheI/EcoRI to remove GFP and inserting mCherry amplified by PCR from 8xGliBS-IVS2-mCherry-NLS-polyA-Tol2 (a gift from James Chen (Addgene plasmid #84604) ...
-
bioRxiv - Developmental Biology 2021Quote: ... using SYBR Premix Ex Taq II (Tli RNaseH Plus) (Takara Bio). For absolute quantification of mRNAs ...
-
bioRxiv - Developmental Biology 2021Quote: ... cDNA was generated from purified total RNA using PrimeScript RT Master Mix (Takara Bio). qRT-PCR was performed on the CFX connect real-time PCR detection system (Bio-Rad ...
-
bioRxiv - Developmental Biology 2021Quote: ... Purified RNA served as input for the cDNA library preparation with SMART-Seq v.4 Ultra Low Input RNA kit (Takara Bio, Kusatsu, Japan) and Nextera XT DNA Library Prep Kit (Illumina ...
-
bioRxiv - Microbiology 2021Quote: ... Products from these two reactions were ligated via In-Fusion HD Cloning Kit (Clontech®, 639650). RSV-GagΔNC (Volker Vogt ...
-
bioRxiv - Microbiology 2021Quote: The complementing strain was constructed by amplifying either nyxA or nyxB and their corresponding promoter region (500 bp upstream) with the PrimeStar DNA polymerase (Takara). Insert and pmini-Tn7 (47 ...
-
bioRxiv - Cell Biology 2021Quote: ... was stably introduced into ChoK1 cells with a pLVX-shRNA1 lentiviral system (Clontech) and selected for with 5 μg/mL of puromycin ...
-
bioRxiv - Genetics 2021Quote: ... and then RT-qPCR was performed using a One Step SYBR PrimeScript PLUS RT-PCR kit (Takara Bio, Shiga, Japan) and Applied Biosystems ABI Prism 7000 Sequence Detection System ...
-
bioRxiv - Genetics 2021Quote: ... Site-directed mutagenesis was performed with the In-Fusion HD kit (Clontech Inc.), creating G34D mutation ...
-
bioRxiv - Genetics 2021Quote: ... 0.025 units of Ex Taq DNA polymerase (TaKaRa, Otsu, Japan), and 1 μL of cDNA ...
-
bioRxiv - Genomics 2021Quote: ... (Takara Bio USA, Inc. Mountain View, CA). Libraries were sequenced in-house using a NextSeq 500 High- Throughput Sequencing System (Illumina San Diego ...
-
bioRxiv - Cell Biology 2021Quote: ... Then the homology arms and fluorescent protein sequences were assembled and cloned into the BamHI site of a pUC19 vector using Gibson assembly (Clontech). The primer sequences for the mGFP-EB1 ...
-
bioRxiv - Cell Biology 2021Quote: ... followed by cloning into a pLVX-puro vector (Clontech) using the XhoI and BamHI restriction sites (primers in Suppl ...
-
bioRxiv - Microbiology 2021Quote: ... Viral RNA was amplified using PrimeScript II High Fidelity One Step RT-PCR Kit (Takara Bio, Shiga, Japan) under the following reaction conditions ...
-
bioRxiv - Microbiology 2021Quote: ... One-Step TB Green PrimeScript PLUS RT-PCR Kit (Takara Bio) was used under the following conditions ...
-
bioRxiv - Microbiology 2021Quote: ... The entire fragment was then infused into a pCAGGS expression vector using an Infusion HD cloning kit (Takara Bio), according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... with the high-fidelity One Step RT-PCR Kit (Takara Bio). PCR products were gel purified and cloned into the pCR4-TOPO vector using the Zero Blunt Topo Cloning Kit (Invitrogen) ...
-
bioRxiv - Microbiology 2021Quote: ... and a linker sequence (5’-GGTAGCGGCAGCGGTAGC-3’) were added through three additional PCR reactions using PrimeStar GXL DNA polymerase (Takara Bio). PCR products were gel-purified in each step ...
-
bioRxiv - Neuroscience 2021Quote: ... 0.4 U/ul RNAse inhibitor (Takara, 2313B), 0.2 U/ul Superasin (Invitrogen ...
-
bioRxiv - Neuroscience 2021Quote: ... Slices were covered with mounting medium (85% Glycerol, 4.4 U/ul of RNAse Inhibitor (Takara, 2313B), 1 mM DTT ...
-
bioRxiv - Immunology 2021Quote: ... polyA-selected transcriptome libraries were constructed using the SMART-seq v4 Ultra Low Input RNA Kit (Takara) combined to the Nextera XT DNA Library Preparation Kit (Illumina ...
-
bioRxiv - Microbiology 2021Quote: ... RT-qPCR was then performed using gene-specific primers (Table S1) and TB Green Premix Ex Taq (Takara). Levels of gene expression were normalized to that of 16S ribosomal RNA gene (OL4498 and OL4449 ...
-
bioRxiv - Microbiology 2021Quote: ... and 1 ng of a luciferase (luc2) containing plasmid (pGL4.26, Clontech) was added to buffer AL prior to lysis ...
-
bioRxiv - Microbiology 2021Quote: ... it was reverse transcribed using the PrimeScript™ RT reagent Kit (TaKaRa). The GPC segment was amplified and sequenced using primers ...
-
bioRxiv - Immunology 2021Quote: ... The isolated mRNA was reverse transcribed to cDNA using PrimeScript RT Reagent Kit (Takara, RR037A). Real-time quantitative PCR was carried out through ABI 7500 Real Time PCR System by SYBR Green Master Mix (YEASEN ...
-
bioRxiv - Microbiology 2021Quote: ... AH109 yeast cells (Clontech; Takara, Mountain View, CA, USA) were transformed with pGAL4-BD-Nmono using a standard lithium-acetate protocol ...
-
bioRxiv - Microbiology 2021Quote: ... AH109 yeast cells (Clontech; Takara, Mountain View, CA, USA) were transformed with pGAL4-BD-Nmono using a standard lithium-acetate protocol ...
-
bioRxiv - Microbiology 2021Quote: ... mouse monoclonal antibody against EGFP (Clontech, Mountain View, CA) was used for western blotting at a dilution of 1:1,000 and rabbit polyclonal antiserum reactive against protein kinase A (PKA ...
-
bioRxiv - Immunology 2021Quote: ... and all subsequent RNA used for cDNA synthesis using the SMART-seq v4 kit (Takara) and 10 cycles of PCR ...
-
bioRxiv - Molecular Biology 2021Quote: ... then directionally cloned into pCMV-HA (Clontech) using the Xho1 and Not1 sites.
-
bioRxiv - Molecular Biology 2021Quote: ... which was cleaved with Xba1 and Cla1 and ~2.6 kbp CMV-IRES-puror Xba1+Cla1 fragment from pQCXIP (Clontech) was inserted to construct pHIV-CMV-IRES-puro ...
-
bioRxiv - Neuroscience 2021Quote: ... rabbit anti-DsRed 1:1000 (Takara, cat# 632496). Samples were washed three times for 5 min in PBT and incubated in Alexa Fluor 488 or 594 secondary antibodies 1:500 (Invitrogen ...
-
bioRxiv - Neuroscience 2021Quote: ... the cpmApple module from jRGECO1a was amplified by PCR and inserted by In-Fusion (Clontech) to the ICL3 region of human DRD1 in the pcDNA5 vector ...
-
bioRxiv - Neuroscience 2021Quote: ... and generated by In-Fusion recombination (Takara Bio). VGAT-SEP was constructed by fusing SEP to the luminal C-terminus of VGAT (NM_031782.2) ...
-
bioRxiv - Neuroscience 2021Quote: ... using CalPhos™ Mammalian Transfection Kit (Takara Bio or Promega) on DIV7 ...