Labshake search
Citations for Takara Bio :
9501 - 9550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... qRT-PCR was performed using first-strand cDNA and a primer pair designed for target genes using the SYBR Premix Ex Taq Perfect Real Time Kit Tli RNAaseH Plus (TAKARA) and Thermal Cycler Dice Real Time System (Model TP800 ...
-
bioRxiv - Molecular Biology 2022Quote: ... with PowerUp™ SYBR™ using the Thermal Cycler Dice® Real Time System III (TAKARA Bio, Japan). These PCRs were run in triplicates ...
-
bioRxiv - Molecular Biology 2022Quote: ... The whole cloning reaction was used to transform Stellar™ chemically competent cells (Clontech® Laboratories) using the heat shock method ...
-
bioRxiv - Molecular Biology 2022Quote: ... The double stranded barcode library was subsequently cloned into the linearized vector using the In-Fusion® HD Cloning Kit (Clontech® Laboratories) following instructions of the manufacturer ...
-
bioRxiv - Molecular Biology 2022Quote: ... Pico Input Mammalian (Takara Bio). m6A RIP was performed with fragmented RNA as previously described by (Zeng et al ...
-
bioRxiv - Molecular Biology 2022Quote: ... 4X Lenti-X Concentrator (TaKaRa 631232) was then added to the filtered virus-containing medium ...
-
bioRxiv - Molecular Biology 2022Quote: ... the amplified gene was cloned into the vector using In-Fusion (TAKARA).
-
bioRxiv - Molecular Biology 2022Quote: ... Library constructions were performed with SMARTer Stranded Total RNA-Seq Kit v2 (TaKaRa, 634417) following the manufacturer’s protocols ...
-
bioRxiv - Molecular Biology 2022Quote: ... total RNA was reverse transcribed by using PrimeScript™ RT Master Mix (Takara, RR0361) with oligo dT primer and random 6 mers as primer ...
-
bioRxiv - Developmental Biology 2022Quote: ... mRNA was captured and amplified with the Smart-seq2 v4 kit (Clontech). After AMPure XP beads purification ...
-
bioRxiv - Cancer Biology 2022Quote: ... Rabbit polyclonal antibodies used were: HECD1 (anti-E-cadherin; Takara), anti-Melan-A (ab15468 ...
-
bioRxiv - Cancer Biology 2022Quote: ... and ligated into the pLVX-Puro vector (Takara #632164) between the PspOMI and BamHI restriction sites by using T4 DNA ligase (New England Biolabs #M0202) ...
-
bioRxiv - Developmental Biology 2022Quote: Full cDNA or specific regions were selected for their comparative variability and cloned using Infusion (Takara) into bacterial expression plasmid (Pet30a) ...
-
bioRxiv - Developmental Biology 2022Quote: ... amplified with SMARTer kit (Clontech), and sequenced on an Illumina HiSeq 4000 platform (100bp runs ...
-
bioRxiv - Developmental Biology 2022Quote: ... Each domain deletion PCR amplification was performed by TAKARA-HIFI Tag by following the manufactory guides ...
-
bioRxiv - Cell Biology 2022Quote: ... Wild-type iRhom2 and iRhom2-1-374 were also cloned by PCR into pM6P.Blasticidin (for retroviral infection) and into pLVX-TetOne-Puro vector (Clontech, #631849) (for lentiviral infection) ...
-
bioRxiv - Cell Biology 2022Quote: Protein extracts were analyzed by SDS-PAGE and immunoblotting using α-GFP (monoclonal, Takara), α-mCherry (polyclonal ...
-
bioRxiv - Cell Biology 2022Quote: ... pAUR112 (TAKARA) carrying the Aureobasidin A resistance gene was digested with BstBI and SmaI to delete the URA3 gene ...
-
bioRxiv - Cell Biology 2022Quote: ... Mav203 was transformed with four plasmids and spread onto Sc-LeuTrpUra plates containing 0.5 μg/ml Aureobasidin A (TAKARA).
-
bioRxiv - Cell Biology 2022Quote: ... full-length GIP-2 cDNA with a C-terminal V5-His6 tag was inserted into pColdI (TAKARA) and used to inoculate rabbits and rats ...
-
bioRxiv - Cell Biology 2022Quote: ... The cDNA was synthesized using a PrimeScriptTM RT reagent Kit (Takara Bio, Beijing, China) according to the manufacturer’s manual ...
-
bioRxiv - Cell Biology 2022Quote: ... BsrGI digested and ligated into a likewise digested C1 (Clontech) backbone ...
-
bioRxiv - Cell Biology 2022Quote: ... the cells were treated with 200 ng ml−1 of Dox (#631311; TaKaRa, Kusatsu, Japan) for 24 h ...
-
bioRxiv - Cancer Biology 2022Quote: ... the complementary DNA (cDNA) products were amplified using TB Green Premix Ex Taq II (Takara Bio Inc, Japan) according to the manufacturer’s instructions in CFX 96 thermo cycler (Bio-Rad Laboratories ...
-
bioRxiv - Cancer Biology 2022Quote: ... in-situ apoptosis detection kit and TB Green Premix Ex Taq II were procured from Takara Bio Inc ...
-
bioRxiv - Cancer Biology 2022Quote: ... and reverse transcribed using PrimeScript RT Reagent kit (Takara Bio Inc, Japan). For qRT-PCR ...
-
bioRxiv - Cancer Biology 2022Quote: ... Lenti-X 293T cells (Takara Bio) were infected using Lipofectamine 3000 (Invitrogen ...
-
bioRxiv - Cancer Biology 2022Quote: ... Infusion Cloning HD Kit was procured from Takara Bio USA Inc ...
-
bioRxiv - Cell Biology 2022Quote: ... was purchased from Genscript and sub-cloned into a lentiviral Tet-On pfTRE3G expression vector (Takara Bio Ltd), modified to contain a triple Flag epitope ...
-
bioRxiv - Microbiology 2022Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...
-
bioRxiv - Microbiology 2022Quote: ... The viral RNA copy number was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). Fluorescent signals were acquired using a QuantStudio 1 Real-Time PCR system (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2022Quote: ... 0.02 g skim milk was added to the lysis buffer to improve the extraction efficiency (Takada-Hoshino and Matsumoto, 2004) and post-elution purification using RNase A (Takara, Shiga, Japan) and DNA Clean & Concentrator (Zymo Research ...
-
bioRxiv - Microbiology 2022Quote: ... and exonuclease I (TaKaRa). The cleaned samples were subjected to Sanger sequencing for strain identification.
-
bioRxiv - Microbiology 2022Quote: ... The concentrated supernatant was passed through an equilibrated TALON Metal Affinity Resin (Takara Bio Inc, Shiga, Japan). The column was washed with 10 volume of PBS containing NaCl (300 mM ...
-
bioRxiv - Microbiology 2022Quote: ... Amplification of each fragment was carried out by using the PrimeSTAR Max DNA polymerase (Takara Bio) in a 50 μL PCR reaction containing 0.2 μM each primer and 1 ng BAC or 2 μL viral cDNA as the template with the cycling condition as follows ...
-
bioRxiv - Microbiology 2022Quote: HEK 293T cells (Clontech) and its derivative ...
-
bioRxiv - Immunology 2022Quote: ... according to the manufacturer’s instructions and was further reverse-transcribed into cDNA through the PrimeScript™ RT Reagent Kit with gDNA Eraser (TaKaRa). A LightCycler 480 II (Roche Applied Science ...
-
bioRxiv - Immunology 2022Quote: The full-length sequences of sea perch RNF34 (GenBank accession number: OP784387) was amplified by PCR using primers (S1 Table) and was subsequently cloned into pCMV-Flag/Myc vector (Clontech). RNF34 deletion mutants (RNF34ΔRING and RNF34ΔZinc ...
-
bioRxiv - Molecular Biology 2022Quote: ... and concentrated 20-fold with Lenti-X Concentrator (Takara). Cell lines were transduced by spinoculation at 500 g for 1 hour at room temperature with 8 μg/mL polybrene (EMD Millipore) ...
-
bioRxiv - Plant Biology 2022Quote: ... The qRT-PCR was performed with the reversed cDNAs as substrates and the MeSWEET10a specific primers (F: 5’-TCCTCACCTTGACTGCGCTG-3’; R: 5’-AGCACCATCTGGACAATCCCA-3’) by using the SYBR Premix Taq Kit (TaKaRa, Dalian, China) in the ABI7500 Real-Time PCR System (Applied Biosystems ...
-
bioRxiv - Plant Biology 2022Quote: ... following the manufacturer’s instructions were reversed by using the reverse transcriptase kit (TaKaRa, Dalian, China). The qRT-PCR was performed with the reversed cDNAs as substrates and the MeSWEET10a specific primers (F ...
-
bioRxiv - Molecular Biology 2022Quote: ... 0.5 µg of quality checked total RNA was converted into first-strand cDNA using a PrimeScript RT Reagent Kit with gDNA Eraser (TaKaRa Bio Inc, Shiga, Japan), according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... and Thermal Cycler Dice Real Time System (Model TP800; TaKaRa Bio Inc). The sequences of the primers for qRT-PCR are shown in Supplemental Table 1 ...
-
bioRxiv - Immunology 2022Quote: ... cerevisiae Y187 (Takara Bio) and Y2HGold (Takara Bio) ...
-
bioRxiv - Immunology 2022Quote: ... Mate & Plate™ cDNA Library in a pGADT7 vector with the GAL4 DNA-activation domain (AD) and pre-transformed into yeast strain Y187 was acquired from Takara Bio ...
-
bioRxiv - Immunology 2022Quote: The recombinant pGBKT7-PGRP3 bait construct was transformed into yeast strain Y2HGold via the lithium acetate method per manufacturer protocol (Takara Bio). Transformants were cultured on SD/-Trp (SDO ...
-
bioRxiv - Immunology 2022Quote: Yeast two-hybrid screening was performed using a GAL4-based system (Matchmaker Gold Yeast Two-Hybrid System; Takara Bio, Mountain View, CA, USA). Assays and screenings were performed according to the manufacturer’s provided user manual ...
-
bioRxiv - Immunology 2022Quote: ... and treated with Exonuclease I (New EnglandBiolabs) before amplification by the Advantage 2 PCR kit (Clontech) and the SINGV6 primer (95°C for 1 min ...
-
bioRxiv - Microbiology 2022Quote: ... Y2H Gold yeast strain is cultured in YPDA (TaKaRa Bio,Shiga, Japan). Escherichia coli strain DH5α is used to preserve the plasmids pRS426 ...
-
bioRxiv - Microbiology 2022Quote: ... The SYBR Premix Ex Taq II kit (TaKaRa) was used for qRT-PCR ...