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Citations for Takara Bio :
9551 - 9600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and cloned into pMD18-T vector (TaKaRa, China) and the recombinant plasmids were picked and sent to Tsingke Biotechnology Co. ...
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bioRxiv - Microbiology 2022Quote: ... First-strand complementary DNA (cDNA) was synthesized with PrimeScript II Reverse Transcriptase (Takara) primed with a gene-specific oligonucleotide ...
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bioRxiv - Microbiology 2022Quote: ... using the primers listed in S-Table 1B into pcDNA3.1-P2A-tagRFP by the In-Fusion HD Cloning Kit (Takara), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and then synthesized and cloned into pRetroX-TRE3G (Takara) by GenScript.
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bioRxiv - Microbiology 2022Quote: ... The Kpn I – BamHI env fragments from the pSVIIIenv plasmids were cloned into the corresponding sites of pE7SB-NL4-3 using Long Ligase (Takara) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... 293T cells were co-transfected with the aforementioned lentiviral vector plasmid and Lentiviral High Titer Packaging Mix (Takara Bio).
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bioRxiv - Microbiology 2022Quote: ... qPCR-confirmed wsp-positive products were cleaned using a mixture of alkaline phosphatase (TaKaRa) and exonuclease I (TaKaRa) ...
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bioRxiv - Microbiology 2022Quote: ... and the supernatant was loaded onto TALON® IMAC resin (Takara Bio) pre-equilibrated with the lysis buffer ...
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bioRxiv - Microbiology 2022Quote: ... The resulting plasmids were used to generate the R mutant plasmid using the TaKaRaMutanBEST Kit (Takara). All strains were validated using the methods described earlier.
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bioRxiv - Microbiology 2022Quote: ... into Lenti-X 293T (Takara Bio) cells using polyethylenimine (PEI ...
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bioRxiv - Microbiology 2022Quote: ... Thermal Cycler Dice Real Time System III (Takara) or 7500 Real-Time PCR System (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... CPER assembly was performed as previously described by combining 0.05 pmol of each fragment in a 50 μL reaction containing 2.5 U PrimeSTAR GXL DNA polymerase (Takara Bio) and using the ‘condition 3’ cycling parameters [18] ...
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bioRxiv - Microbiology 2022Quote: ... 0.8 U/μl recombinant RNase inhibitor (Takara, Cat# 2313B)] and incubated at room temperature for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... total RNA was extracted using the TRIzol® reagent (R0016, Beyotime, Shanghai) followed by the removal of genomic DNA using DNase I (TaKara). The 16S and 23S ribosomal RNA (rRNA ...
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bioRxiv - Immunology 2022Quote: ... Clarified culture supernatants were purified over Cobalt-TALON affinity resin (Takara), with subsequent purification via size exclusion chromatography (SEC ...
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bioRxiv - Immunology 2022Quote: ... Cobalt-TALON resin (Takara) was used to perform immobilized metal affinity chromatography via the 8xHis tag ...
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bioRxiv - Immunology 2022Quote: FACS sorted cells (5,000 cells) were subjected to cDNA synthesis using SMART-Seq v4 Ultra Low Input RNA Kit for sequencing (Takara/Clontech). Barcoded libraries were generated by the Nextera XT DNA Library Preparation kit (Illumina ...
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bioRxiv - Immunology 2022Quote: ... Virus titer was determined by using Jurkat T cells and Lenti-X GoStix Plus (Takara Clontech).
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bioRxiv - Immunology 2022Quote: FACS sorted cells (5,000 cells) were subjected to cDNA synthesis using SMART-Seq v4 Ultra Low Input RNA Kit for sequencing (Takara/Clontech). Barcoded libraries were generated by the Nextera XT DNA Library Preparation kit (Illumina ...
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bioRxiv - Immunology 2022Quote: ... Virus titer was determined by using Jurkat T cells and Lenti-X GoStix Plus (Takara Clontech).
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bioRxiv - Immunology 2022Quote: ... The SMARTer Mouse TCR a/b Profiling Kit (Takara, Cat. No. 634403) was used to amplify both TCRα and TCRβ sequences ...
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bioRxiv - Immunology 2022Quote: ... cDNA was synthesized from DNase I-treated RNA using SMARTScribe™ Reverse Transcriptase (Clontech) with 0.2 μM each of gene-specific reverse primers (same as above ...
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bioRxiv - Immunology 2022Quote: ... before amplification using the Advantage 2 PCR kit (Clontech) and the SINGV6 primer (95°C for 1 min ...
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bioRxiv - Immunology 2022Quote: ... followed by In-Fusion (Takara) cloning with EcoRI/XhoI cut sites ...
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bioRxiv - Immunology 2022Quote: ... Sequences encoding DCFHP (residues 1-1146 of HexaPro)2 and SΔC-Fer (residues 1-1143 as previously described)3 were cloned into the pADD2 vector backbone using HiFi PCR (Takara) followed by In-Fusion (Takara ...
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bioRxiv - Immunology 2022Quote: ... CD34+ cells were cultured overnight on RetroNectin-coated plates (Takara, T100B, Mountain View, CA) in X-VIVO 10 media (Lonza ...
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The arms race in noncoding regions of transposable element: the evolution of anti-silencing and RNAibioRxiv - Genetics 2022Quote: ... Converted DNA was used as a template for PCR using EpiTaq HS (TaKaRa). The PCR conditions were 94 °C for 1 min ...
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bioRxiv - Immunology 2022Quote: ... and the transmembrane domain replaced with a GCN448 or foldon trimerization49 domain followed by an Avi-Tag50 and a hexahistidine tag was cloned into a mammalian expression vector (pADD2) by In-Fusion (Takara Bio). Similarly ...
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bioRxiv - Plant Biology 2022Quote: ... The coding sequences of PWO2 and the fragment of PWO3 were cloned in the pGBKT7 and pGADT7 vectors (Clontech), passing through pDONR221 (Invitrogen ...
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bioRxiv - Plant Biology 2022Quote: The yeast strain Y2HGold and the Matchmaker Gold Yeast Two-Hybrid System (Clontech) were used for the yeast two-hybrid assay ...
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bioRxiv - Plant Biology 2022Quote: ... the full-length coding sequence of PLP with His6 at the N terminus was amplified by PCR and inserted into SalI-digested pBYR2HS vector using the In-Fusion Snap Assembly Master Mix (Takara Bio). The leaves of four-week-old N ...
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bioRxiv - Microbiology 2022Quote: ... and PCR inhibitors were removed from the DNA extracts using CHROMA SPIN+TE-400 Columns (Takara Bio, Inc.) and Amicon Ultra-0.5 (100K ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Amplified products were purified using NucleoSpin Gel and PCR Clean-up (Takara Bio), and cloned into the pGEM-T easy vector (Promega) ...
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bioRxiv - Immunology 2022Quote: ... and Y2HGold (Takara Bio). Additional information regarding their genotype and reporters are provided in Table 1.
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bioRxiv - Cell Biology 2022Quote: ... Reverse transcription was performed with 10 ng RNA using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Steps were performed on an automated system (Apollo 342, Wafergen Biosystems/Takara). After a final PCR amplification step ...
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bioRxiv - Cell Biology 2022Quote: ... using an MSND device (Takara Bio). Cell dispensing ...
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bioRxiv - Microbiology 2022Quote: ... The resulting plasmid was transfected into 293T cells using Lentiviral High Titer Packaging Mix (Takara) to obtain the lentiviral vector for TSG101–venus transduction ...
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bioRxiv - Neuroscience 2022Quote: The cDNA samples were prepared using a Prime-Script RT reagent Kit (TaKaRa, Dalian, China) according to the manufacturer’s instructions ...
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Neural circuit-wide analysis of gene expression during deafening-induced destabilization of birdsongbioRxiv - Neuroscience 2022Quote: ... 0.25 μL Recombinant Ribonuclease Inhibitor (Takara), and 10 U/μL Enzscript Moloney-Murine Leukemia Virus Reverse Transcriptase (Enzymatics) ...
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bioRxiv - Genetics 2022Quote: ... cDNA was synthesized using the PrimeScript RT Reagent Kit (Perfect Real Time, Takara Bio), and quantitative real-time-PCRs were performed on an AriaMx Real-Time PCR System (Agilent ...
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bioRxiv - Neuroscience 2022Quote: ... eGFP (Clontech), pCAG_GPHN.FingR-eGFP-CCR5TC [23] (gift from Don Arnold ...
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bioRxiv - Systems Biology 2022Quote: ... rabbit dsRed (1:500, Clonetech Takara 632496 to detect Tomato protein); mouse βTubulin (1:600 ...
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bioRxiv - Molecular Biology 2022Quote: ... The amplified fragments required to generate the chimeric receptor were combined with a linearized pCS2+ backbone and annealed using an In-Fusion kit (Takara Bio, 638947). The resulting plasmids were transformed into Top10 chemically competent cells ...
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bioRxiv - Molecular Biology 2022Quote: ... Pico Input Mammalian (Takara Bio). All the libraries were single-end sequenced (1X 88 bp ...
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bioRxiv - Cancer Biology 2022Quote: Samples were then processed using the SMART-seq Stranded kit (Cat. 634444, protocol version 051018; Takara Bio USA, Inc.) according to the manufacturer’s protocol with minor modifications ...
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bioRxiv - Cell Biology 2022Quote: ... using an In-Fusion HD Cloning kit (Takara Bio) and sequenced (GeneWiz ...
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bioRxiv - Microbiology 2022Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...
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bioRxiv - Systems Biology 2022Quote: ... with pMI-NLS-Cre-ires-Tomato plasmid using the CalPhos kit (Clontech). Retroviral supernatants were harvested after 48hrs ...
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bioRxiv - Plant Biology 2022Quote: ... All experimental processes were operated according to protocol (Clontech).