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Citations for Takara Bio :
9751 - 9800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... CA mutations were made using the In-Fusion cloning system (Takara Bio) using either custom Gene Blocks (Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... Rabbit anti-Dsred (Takara, Cat# 632496), Goat anti-GFP (Abcam ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cloned vectors were transformed into Stellar cells (Takara) before plasmid purification using a miniprep kit (NEB) ...
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bioRxiv - Immunology 2022Quote: ... First-strand cDNA was synthesized using the PrimeScript™ RT Kit (TaKaRa, Beijing, China) and used as a template to determine the expression of RBD genes with the indicated primers and probes ...
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bioRxiv - Immunology 2022Quote: ... using Premix Ex Taq™ (Probe qPCR) reagent (TaKaRa, Beijing, China) in qRT-PCR ...
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bioRxiv - Immunology 2022Quote: ... StellarTM and BL21(DE3) competent cells were purchased from Takara Bio and Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... coated with 30 μg/mL Retronectin reused up to three times (T100B, Takara), at 2000 x g for 2 hours at 32°C and replaced with activated human CD8+ T cells in fresh RPMI supplemented with 30 U/mL IL-2 ...
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bioRxiv - Immunology 2022Quote: ... Lentiviral particles were produced in Lenti-X cells (Clontech) per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and 2.2 μg pVSVG (Clontech. Cat# 631247) cDNAs ...
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bioRxiv - Biochemistry 2022Quote: ... using SYBR Premix (Takara, Dalian, China). The relative amount of lys-7 or lys-8 mRNA to act-1 mRNA (an internal control ...
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bioRxiv - Cell Biology 2022Quote: ... and the PCR product was subcloned into the pcDNA3.1 (+) C-terminal PA vector (digested with KpnI/XhoI) using an In-Fusion HD Cloning Kit (#Z9648N; TaKaRa, Kusatsu, Japan), resulting in pcDNA3.1(+ ...
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bioRxiv - Biochemistry 2022Quote: ... and concentrated by adding Lenti-X concentrator (Takara Bio) at a 1:3 ratio and incubating at 4°C for at least 1 h ...
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bioRxiv - Synthetic Biology 2021Quote: ... DmrA and DmrC were amplified and then fused to each split-Cas9 (N_Cas9 and C_Cas9) using the In-Fusion HD Cloning Kit (Clontech). Links to sequences of all the generated plasmids are listed in Supplementary Table 3.
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bioRxiv - Synthetic Biology 2021Quote: ... pCR-U6-gRNA-TRE3G-TdTomato and -Nluc were generated by replacing the miniCMV promoter of lenti sgRNA(MS2)_puro-miniCMV-TdTomato/Nluc with the TRE3G promoter from pTRE3G (Takara, #631173) using Gibson Assembly and inserting into pCR Blunt II-TOPO ...
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bioRxiv - Synthetic Biology 2021Quote: ... cDNA was synthesized using the PrimeScript RT Master Mix (Takara, #RR036A) by following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... the supernatant was applied to cobalt sepharose (Talon®, 635502, Clontech). The column was washed four times with 40 ml of 35 mM imidazole in PBS ...
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bioRxiv - Biophysics 2021Quote: ... pEGFP-N1 (BD Biosciences Clontech, USA) was cotransfected with the channel DNA to visualize successfully transfected cells via their green fluorescence ...
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bioRxiv - Plant Biology 2021Quote: Yeast strain AH109 was transformed with bait (pGBKT7) and prey (pGADT7) constructs by following the small scale yeast transformation protocol from Yeastmaker™ Yeast Transformation System 2 (Clontech). Upon transformation ...
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bioRxiv - Plant Biology 2021Quote: ... Cloning procedure was performed using In-Fusion HD cloning kit (Takara Bio USA). Constructs for GFP and mCherry upstream or downstream of the Gateway cassette were amplified using primers GW_RfA_mCherry-F ...
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bioRxiv - Plant Biology 2021Quote: ... pGADT7-GW and pGBKT7-GW have been modified from pGADT7 and pGBKT7 (Clontech) into a Gateway-compatible form using Gateway™ Vector Conversion System (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... RNA was synthetized using the T7-Scribe transcription kit (Cellscript) and purified with Chroma Spin DEPC-H2O columns (Clontech, CA, USA). The ncAA naphthalene was ligated to the tRNA with T4 DNA ligase (New England Biolabs ...
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bioRxiv - Biophysics 2021Quote: ... Five plasmids designed to express the following “chaperone teams” were purchased from TAKARA Bio Inc ...
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bioRxiv - Biophysics 2021Quote: ... Constructs containing both His and Strep tags were purified using gravity flow columns containing His60 Ni-NTA resin (Clontech) followed by Streptactin affinity chromatography (IBA Lifesciences ...
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bioRxiv - Biophysics 2021Quote: ... The first-strand cDNA was synthesized using a PrimeScript® RT reagent Kit (Takara, Otsu, Shiga, Japan). The PlMYB308 gene was PCR-amplified using specific primers (Table S6) ...
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bioRxiv - Biophysics 2021Quote: ... SYBR Premix Ex Taq II (Takara, Otsu, Shiga, Japan) on StepOnePlus Real Time PCR system (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... and anti-mCherry (#Z2496N, TaKaRa, Shiga, Japan).
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bioRxiv - Biophysics 2021Quote: The SNAP-eGFP fusion construct was created by amplifying the SNAP fragment from pIRES-PEX5-SNAP/eGFP-SKL with the primers KR011/KR012 and subcloning it into HindIII/BamHI digested peGFP-N1 (Clontech).
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bioRxiv - Biophysics 2021Quote: The PEX5L-SNAP fusion construct was created by amplifying the SNAP fragment from pIRES-PEX5-SNAP/eGFP-SKL with the primers KR022/KR026 and subcloning it into SalI/NotI digested peGFP-N1 (Clontech).
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bioRxiv - Biophysics 2021Quote: ... which was digested with XhoI and BamHI restriction enzymes from the EYFP-actin vector (#6902-1, Clontech) into the mClover2-C1 vector (Addgene plasmid #54577 ...
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bioRxiv - Biophysics 2021Quote: ... Solubilized NaK was then purified via IMAC using Talon cobalt affinity resin (Clontech). For the K+ sample ...
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bioRxiv - Cancer Biology 2021Quote: ... 24 cycles (for actin beta [ACTB]) or 35 cycles (for MLKL) of amplification were performed under the following conditions using PrimeSTAR Max DNA Polymerase (Takara): denaturing at 98°C for 10 s ...
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bioRxiv - Cancer Biology 2021Quote: ... qRT-PCR was performed using SYBR® Premix Ex Taq™ (Takara, Japan) and 300 nmol/L of each primer ...
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bioRxiv - Cancer Biology 2021Quote: ... The promoter regions of target genes were amplified and quantified by qRT-PCR using SYBR Green (Takara, Japan) on an ABI 7500 Fast system ...
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bioRxiv - Cancer Biology 2021Quote: ... at a molar ratio of 2:1:1 and transfected into the Lenti-X™ 293T packaging cell line (632180, TaKaRa Bio Inc., Kusatsu, Shiga, Japan) using Xfect™ Transfection Reagent (631317 ...
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bioRxiv - Cancer Biology 2021Quote: ... using Xfect™ Transfection Reagent (631317, TaKaRa Bio Inc., Kusatsu, Shiga, Japan) following manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2021Quote: ... Retroviruses encoding a second-generation CAR targeting HER-2 was obtained from the supernatant of the GP+E86 packaging line spun together with T cells onto RetroNectin-coated (10 μg/ml) 6-well plates (Takara Bio) and incubated overnight before the second viral transduction ...
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bioRxiv - Cancer Biology 2021Quote: ... and concentrated using a Lenti-X™ Concentrator (631231, TaKaRa Bio Inc., Kusatsu, Shiga, Japan). Mia PaCa-2 cells and PCAFs were then transduced with the respective viral suspension at different multiplicity of infection (MOI ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was isolated using RNAiso Plus reagent from TaKaRa (TaKaRa, 9108) as mentioned in the product manual ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was isolated using RNAiso Plus reagent from TaKaRa (TaKaRa, 9108) as mentioned in the product manual ...
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bioRxiv - Cancer Biology 2021Quote: ... Spodoptera frugiperda Sf21 cells (Clontech) were propagated in Grace’s medium (Gibco ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... Eluted RNA was used for library preparation using SMARTer smRNA-Seq Kit (Takara), with following modifications ...
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bioRxiv - Cancer Biology 2021Quote: ... NRF2 and SLC7A11 was obtained by In-fusion cloning (Takara), named pDMP-T5 ...
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bioRxiv - Cancer Biology 2021Quote: RNA was extracted using the nucleospin RNA kit (Takara) according to the manufacturer’s recommended protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... The collected media containing virus was filtered through 0.45μm pore filter and mixed with Lenti-X concentrator (Clontech) as indicated by manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA was converted to cDNA using the PrimeScript RT reagent Kit (Takara, RR037A).
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: RNA-seq libraries (used for calculating translation efficiencies) were prepared using SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara), with 50 ng total RNA as input ...
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bioRxiv - Cancer Biology 2021Quote: ... Spodoptera frugiperda Sf21 cells (Clontech) were propagated in Grace’s medium (Gibco ...
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bioRxiv - Biophysics 2021Quote: ... supplemented with 10% tetracycline-free fetal bovine serum (FBS; Takara Bio) and 6 mM L-glutamine ...
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bioRxiv - Biochemistry 2021Quote: ... A total of 5 μg of RNA were reverse transcribed and amplified using One Step SYBR Prime-Script PLUS RT-PCR Kit (TaKaRa) and the Thermal Cycler Dice instrument (TaKaRa ...
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bioRxiv - Genetics 2021Quote: ... viral supernatant was collected and concentrated using Retro-X (Clontech). HeLa TGS1-mutant clones were transduced in the presence of 5 μg/mL polybrene and selected in 2 μg/mL puromycin.