Labshake search
Citations for Takara Bio :
9801 - 9850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ChIP-seq libraries were generated from ChIP-DNA using a custom Illumina library type on an automated system (Apollo 342, Wafergen Biosystems/Takara, Active Motif). ChIP-seq libraries were sequenced on Illumina NextSeq 500 as single-end 75bp reads (Active Motif).
-
bioRxiv - Neuroscience 2021Quote: ChIP-seq libraries were generated from ChIP-DNA using a custom Illumina library type on an automated system (Apollo 342, Wafergen Biosystems/Takara). ChIP-seq libraries were sequenced on Illumina NextSeq 500 as single-end 75bp reads (Active Motif).
-
bioRxiv - Biochemistry 2021Quote: ... Cfr was purified by Talon chromatography (Clontech) from clarified lysates ...
-
bioRxiv - Biochemistry 2021Quote: ... Living Colors (GFP) (JL-8, 632380, Clontech), α-Tubulin (DM1A ...
-
bioRxiv - Biochemistry 2021Quote: ... then incubated at 4 °C in Lenti-X Concentrator (Clontech) for 1 h ...
-
bioRxiv - Biochemistry 2021Quote: ... Reverse transcription kit was purchased from TaKaRa, Japan.SYBR Green PCR kit was purchased from American Applied Biosystems ...
-
bioRxiv - Biochemistry 2021Quote: ... and the Thermal Cycler Dice instrument (TaKaRa) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... converted to cDNA by using the Prime Script reverse transcriptase (Takara, Shiga, Japan) as per the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... and used for quantitative real-time PCR amplification using SYBR Green (Takara). Target RNA expression was normalized to β2m mRNA ...
-
bioRxiv - Microbiology 2021Quote: ... UK) containing the 27F/1492R primer set and MightyAmp DNA polymerase Ver.3 (Takara Bio). PCR was performed according to a previous report (Fujiyoshi et al ...
-
bioRxiv - Cancer Biology 2021Quote: ... with SYBR Premix EX Taq kit (Takara Biotechnology Co., Ltd.). Primer sequences for RRM2 ...
-
bioRxiv - Cancer Biology 2021Quote: ... human MSI1 cDNA was amplified with the primers of MSI1_BamHI-F and MSI1_stopdead_XbaI-R (Table S6) using the PrimeScriptTM 1st strand cDNA Synthesis (Takara, 6210A) and PrimeSTAR ® Max DNA Polymerase kits according to manufacturer instructions (Takara ...
-
bioRxiv - Cancer Biology 2021Quote: ... and PrimeSTAR ® Max DNA Polymerase kits according to manufacturer instructions (Takara, R045A). The PCR product was cloned into the pENTR4 no ccDB plasmid (Addgene #17424 ...
-
bioRxiv - Cancer Biology 2021Quote: ... HEK293T Lenti-X cells were purchased from Takara. Primary mouse melanoma cell lines were isolated from mouse melanomas ...
-
bioRxiv - Cancer Biology 2021Quote: ... First-strand cDNA was generated with PrimeScript RT Master Mix (Takara). qPCR was performed on StepOnePlus™ Real-Time PCR System (Thermo Fisher) ...
-
bioRxiv - Cancer Biology 2021Quote: ... or PrimeScrip RT Master Mix (Takara) (mRNA) ...
-
bioRxiv - Cancer Biology 2021Quote: ... After purification of the His6-SUMO-binder fusion proteins using TALON metal affinity resin (Clontech), they were digested with SUMO protease 1 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Supernatant was collected at 48- and 72-hours post transfection and virus was concentrated using Lenti-X™ concentrator (Takara) and resuspended in appropriate volumes of MEM ...
-
bioRxiv - Cancer Biology 2021Quote: ... (Takara)(Figure S1D) ...
-
bioRxiv - Cancer Biology 2021Quote: ... The multiple cloning site of a pIRES2-AcGFP1 plasmid vector (Takara Bio USA ...
-
bioRxiv - Cancer Biology 2021Quote: ... which ultimately was produced with Takara’s Lenti-X™ 293T cells (Clontech, cat. no. 632180) per the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... constructed after Creb1 cDNA introduction into a pCMVβ vector (Clontech, CA, #631719). Primary LUAD cells from C57BL/6 and FVB mice as well as Trp53- conditional LUAD cells were generated as described elsewhere (3 ...
-
bioRxiv - Cancer Biology 2021Quote: ... The viral supernatant was then concentrated 20-fold with Lenti-X Concentrator (Clontech) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: Full-length cDNA was synthesized from total RNA (1 μg) by SMARTer®□ PCR cDNA Synthesis kit (Clontech) according to the manufacture’s instruction ...
-
bioRxiv - Cancer Biology 2021Quote: ... a Prime Script RT kit (Takara, Tokyo, Japan) was adopted for the generation of complementary DNA ...
-
bioRxiv - Cancer Biology 2021Quote: Illumina sequencing libraries of isolated RPFs was performed with the SMARTer smRNA-Seq Kit for Illumina (Takara Bio). Library preparation was performed according to the manufacturer’s protocol with the following parameters ...
-
bioRxiv - Cancer Biology 2021Quote: ... QPCR reactions were performed according to the manufacturer’s instructions using SYBR® Premix Ex Taq kit (TaKaRa). All reactions were performed in triplicates ...
-
bioRxiv - Cancer Biology 2021Quote: ... cDNA was then prepared using the PrimeScript RT reagent kit (TaKaRa). QPCR reactions were performed according to the manufacturer’s instructions using SYBR® Premix Ex Taq kit (TaKaRa) ...
-
bioRxiv - Cancer Biology 2021Quote: ... containing 2 μL of PBS including 0.2% Triton X-100 and 4U of RNase inhibitor (Takara) per well ...
-
bioRxiv - Cancer Biology 2021Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was carried out using the TB GreenTM Premix Ex TaqTM II (Takara Bio Inc., Kusatsu, Japan) according to the manufacturer’s protocol for a total reaction volume of 20 μl ...
-
bioRxiv - Cancer Biology 2021Quote: ... Reverse transcription was done using the PrimeScript™ RT reagent Kit (Takara) according to the manufacturer’s recommendation ...
-
bioRxiv - Cancer Biology 2021Quote: ... and concentrated using Lenti-X Concentrator (Clontech) as per the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... Virus was collected at 48 hours and 72 hours and concentrated with Lenti-X Concentrator (Clontech). The Virus was resuspended in DMEM and stored at -80C.
-
bioRxiv - Cancer Biology 2021Quote: ... 5.0 μl Fast SYBR Green Master Mix (SYBR premix EX Taq, TaKaRa). The fluorescence was measured at the end of each cycle ...
-
bioRxiv - Cancer Biology 2021Quote: ... 10 ng of RNA was used to prepare cDNA sequencing libraries with the Clontech SMARTer Stranded Total RNA-Seq kit (Pico Input) with ribosomal cDNA depletion (Takara Bio USA, Cat #634413). Prepared libraries were submitted to paired-end 100 bp sequencing on a NovaSeq 6000 system at the YCGA ...
-
bioRxiv - Cancer Biology 2021Quote: ... RNA-seq libraries were prepared with SMART-Seq® Stranded Kit (Takara-Clontech). Samples were sequenced in wholeRNAseq using the NextSeq illumina 500 Platform (75 bp single read) ...
-
bioRxiv - Cancer Biology 2021Quote: ... RNA-seq libraries were prepared with SMART-Seq® Stranded Kit (Takara-Clontech). Samples were sequenced in wholeRNAseq using the NextSeq illumina 500 Platform (75 bp single read) ...
-
bioRxiv - Cancer Biology 2021Quote: Second strand synthesis and PCR amplification was done by adding the Advantage 2 Polymerase Mix (Clontech) and the SINGV6 primer (10 pmol ...
-
bioRxiv - Cancer Biology 2021Quote: Knockdown of endogenous gene expression was performed using the RNAi-Ready pSIREN-RetroQ Vector (Clontech). Cells were retrovirally transfected and selected with puromycin ...
-
bioRxiv - Cell Biology 2021Quote: Both mAlkbh5 and mAlkbh5-HAtag were amplified from the cDNA using gateway forward and reverse primer using PrimeSTAR GXL DNA Polymerase (Takahara Clontech # R050A-TAK), and the PCR product was purified using QIAquick PCR Purification Kit (Qiagen #28106) ...
-
bioRxiv - Cell Biology 2021Quote: LentiX 293T cells were used for preparation of lentivirus (Takahara Clontech #632180). Lentix 293T cells were plated at 1×106 cells per 10 cm dish in DMEM%10FBS (tetracycline free FBS PANSera catalog ...
-
bioRxiv - Cell Biology 2021Quote: ... The cyclin D1-GFP plasmids were constructed by combining the PCR fragment of cyclin D1 from the cyclin D1-Flag plasmid and GFP from pEGFP-N1 (BD Biosciences Clontech) into XPack CMV constructs (System Biosciences) ...
-
bioRxiv - Cell Biology 2021Quote: ... The XPack-GFP plasmid was generated by inserting GFP from pEGFP-N1 (BD Biosciences Clontech) into the XPack CMV-XP-MCS-EF1α-Puro Cloning Lentivector (System Biosciences ...
-
bioRxiv - Neuroscience 2021Quote: ... Library generation was performed using the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (cat no. 634412, Takara Bio Inc), then submitted to the MIT BioMicro Center for quality control (fragment analysis and qPCR) ...
-
bioRxiv - Neuroscience 2021Quote: ... rabbit anti-dsred (1/1000; TaKaRa), mouse anti-acetylated Tubulin (1/500 ...
-
bioRxiv - Cancer Biology 2021Quote: ... and TCR beta chain libraries were generated using SMARTer Mouse TCR a/b Profiling Kit (Clontech). Samples were pooled to a final pool concentration of 4 nM and diluted to a final concentration of 13.5 pM ...
-
bioRxiv - Cancer Biology 2021Quote: ... The TRE3G promoter was amplified from the pTRE3G vector (Clontech) and inserted into SpeI/EcoRI sites of pCDH-EF1-Cre.
-
bioRxiv - Cancer Biology 2021Quote: ... Reverse-transcription was performed using the PrimeScriptTMRT Master Mix Reagent Kit (Takara, Cat. #RR036A) and quantitative real-time PCR was performed using the SYBR® Premix Ex Taq II Reagent Kit (Takara ...
-
bioRxiv - Cancer Biology 2021Quote: ... and quantitative real-time PCR was performed using the SYBR® Premix Ex Taq II Reagent Kit (Takara, Cat. Cat. #RR820A) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2021Quote: The indicated cell lines were obtained from CCLE and were modified to express dCas9-KRAB-BFP and the Tet Responsive element (TEG3G, Takara), the PAX8 overexpression constructs in vector pMT02558 ...