Labshake search
Citations for Takara Bio :
9651 - 9700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The PCR products were subcloned into the pcDNA5 FRT/TO HA FLAG Ms Jaw1 (digested with HpaI/BamHI) using an In-Fusion HD Cloning Kit (#Z9648N; TaKaRa, Kusatsu, Japan). Similarly ...
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bioRxiv - Cell Biology 2022Quote: ... and the DNA fragments were ligated to the pcDNA5 FRT/TO HA FLAG vector (digested with the same enzymes) using a DNA Ligation Kit (#6023; TaKaRa, Kusatsu, Japan), resulting in pcDNA5 FRT/TO HA FLAG Hu sec11a and pcDNA5 FRT/TO HA FLAG Hu sec11c ...
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bioRxiv - Cell Biology 2022Quote: ... by double-insert cloning using an In-Fusion HD Cloning Kit (#Z9648N; TaKaRa, Kusatsu, Japan). The pcDNA5 FRT/TO HA FLAG Hu AASS was then digested with BamHI/ApaI and the DNA fragment was ligated into the pcDNA5 FRT/TO vector using a DNA Ligation Kit (#6023 ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR products were subcloned into the pcDNA5 FRT/TO HA FLAG Ms Jaw1 (digested with AfeI/BamHI) using an In-Fusion HD Cloning Kit (#Z9648N; TaKaRa, Kusatsu, Japan) to swap the luminal region of Jaw1 to that of KASH proteins ...
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bioRxiv - Cell Biology 2022Quote: ... and the PCR product was subcloned into the pcDNA3.1(+) Ms Jaw1 PA (digested with XhoI) using an In-Fusion HD Cloning Kit (#Z9648N; TaKaRa, Kusatsu, Japan), resulting in pcDNA3.1(+ ...
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bioRxiv - Cell Biology 2022Quote: ... GEM-Halo-FRB was labelled with Halo-TMR and then treated with rapalog (TAKARA) for 15 min ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA (400 ng) was then reverse-transcribed using PrimeScript RT Master Mix (#RR036A; TaKaRa, Kusatsu, Japan). The cDNA was then subjected to the subsequent PCR using TB Green Premix Ex™ Taq II (Tli RNaseH Plus ...
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bioRxiv - Cell Biology 2022Quote: ... or Thermal Cycler Dice Real Time System (TP800; TaKaRa, Kusatsu, Japan), using the following primers ...
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bioRxiv - Cell Biology 2022Quote: ... and the DNA fragments were ligated into the pcDNA5 FRT/TO HA FLAG vector (digested with same enzymes) using a DNA Ligation Kit (#6023; TaKaRa, Kusatsu, Japan), resulting in pcDNA5 FRT/TO HA FLAG Ms Jaw1 PA and pcDNA5 FRT/TO HA FLAG Ms Jaw1 NIDR PA ...
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bioRxiv - Cell Biology 2022Quote: ... using a DNA Ligation Kit (#6023; TaKaRa, Kusatsu, Japan). For the cloning of the sec11a (NM_014300.3 ...
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bioRxiv - Cell Biology 2022Quote: ... The pcDNA5 FRT/TO HA FLAG Hu AASS was then digested with BamHI/ApaI and the DNA fragment was ligated into the pcDNA5 FRT/TO vector using a DNA Ligation Kit (#6023; TaKaRa, Kusatsu, Japan), resulting in pcDNA5 FRT/TO Hu AASS ...
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bioRxiv - Cell Biology 2022Quote: ... C-terminal PA vector was first prepared by insertion of a DNA cassette encoding the PA tag (annealed primer set 28) into the pcDNA3.1(+) vector (digested with XhoI/XbaI) using a DNA Ligation Kit (#6023; TaKaRa, Kusatsu, Japan). The DNA fragment coding for Ms Jaw1 was then amplified by PCR with primer set 29 from pTagGFP2-C Ms Jaw1 (previously described by Kozono et al. ...
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bioRxiv - Cell Biology 2022Quote: ... mouse anti-GFP (Clone JL-8; Clontech) at 1:1,000 ...
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bioRxiv - Cell Biology 2022Quote: ... were run onto 8% SDS- polyacrylamide gels that were transferred to nitrocellulose membranes which were reacted with α-His tag antibody (#631212, Clontech). Quantitation of band intensities was done with ImageLab software (BioRad) ...
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bioRxiv - Cell Biology 2022Quote: ... on a Thermal Cycler Dice Real Time System II MRQ (TP960; TaKaRa, Kusatsu, Japan) or Thermal Cycler Dice Real Time System (TP800 ...
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bioRxiv - Cell Biology 2022Quote: ... and total RNA was isolated using RNAiso Plus (#9108; TaKaRa, Kusatsu, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The cDNA was then subjected to the subsequent PCR using TB Green Premix Ex™ Taq II (Tli RNaseH Plus) (#RR820A; TaKaRa, Kusatsu, Japan) on a Thermal Cycler Dice Real Time System II MRQ (TP960 ...
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bioRxiv - Cell Biology 2022Quote: ... the presence of lentivirus was confirmed using Lenti-X GoStix (TaKaRa). Viral supernatants were supplemented with a mixture of 60% DMEM/40% FBS and stored at 4ºC if to be used within the next 48 hours or frozen at -80ºC until use ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T expressing either iRhom2-1-374 or wild-type iRhom2 under TET-inducible cells were generated by lentiviral infection using gene cloned into pLVX-TetOne vector (Clontech, #631849). Methodology is similar to retroviral transduction ...
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bioRxiv - Biophysics 2022Quote: ... All chimeras and mutations were generated using In-Fusion Cloning (Takara Bio) and were confirmed by Sanger sequencing (Genewiz ...
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bioRxiv - Cell Biology 2022Quote: ... PtK2 cells were transfected with eGFP-α-tubulin (Clontech) using Viafect (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... and InFusion HD cloning according to manufacturer’s protocol (Takara Bio, #639649). Site-directed mutagenesis (SDM ...
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bioRxiv - Cancer Biology 2022Quote: ... the coding region of RAS cDNA was first PCR-amplified from the plasmids above by using CloneAmp HiFi PCR Premix (Takara #639298) and the primers listed in Supplementary Table 3 using manufacturer’s recommended PCR conditions ...
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bioRxiv - Cancer Biology 2022Quote: ... The ligation reaction was purified by ethanol precipitation and transformed into Stellar Competent Cells (Clontech) to assess library diversity by Sanger sequencing of 10 clones ...
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bioRxiv - Cell Biology 2022Quote: ... SMART-Seq® HT PLUS Kit (Takara, Kusatsu, Japan) was used to build mRNA libraries ...
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bioRxiv - Cell Biology 2022Quote: ... with the SYBR premeraseTaq kit (TAKARA). Amplified mRNA levels were normalized to 18s mRNA ...
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bioRxiv - Cell Biology 2022Quote: MCF-7 and T47D cells were harvested and total RNA was extracted with RNA Trizol (TAKARA) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of total RNA was reversely transcribed into cDNA using the PimeScript RT-PCR kit (TAKARA) and analyzed by qPCR on LightCycler96 system (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... The viral supernatant was passed through a 0.45 um filter and concentrated to 1:66 with Lenti-X concentrator (631231, Takara Bio) in house or to 1:255 by ultracentrifugation in the core.
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bioRxiv - Cell Biology 2022Quote: ... and 1.5 μL Titanium Taq polymerase (Takara,# 639242). The following thermocycler conditions were used ...
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bioRxiv - Cell Biology 2022Quote: ... we additionally concentrated the lentivirus approximately 60-fold using Lenti-X Concentrator (Takara Bio Inc., #631231).
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bioRxiv - Biophysics 2022Quote: ... These plasmids were designed by fusing the coding sequences of the respective proteins C-terminal (intracellularly) via a linker to mEGFP (called D0 / Donor only) or mCherry in the pIRESpuro2 vector (Clontech)32 (for more linker details see Supplementary Table 5) ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNAs from the H1 and H2 hybridomas were purified using the NucleoSpin RNA purification kit (TAKARA, Tokyo, Japan) as described in the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... 5’ RACE was performed using the SMARTer 5’/3’ kit (TAKARA) with slight modifications ...
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bioRxiv - Biophysics 2022Quote: ... YeastMaker DNA carrier 50 μg (Takara Bio), supplemented with 1 μg of pYeDP60 vector) ...
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bioRxiv - Cell Biology 2022Quote: ... and concentrated by adding Lenti-X concentrator (Takara Bio) at a 1:3 ratio and incubating at 4°C for at least 1 h ...
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bioRxiv - Cancer Biology 2022Quote: ... total RNA from all of the experimental groups was isolated using RNAiso Plus (Takara, Japan). Complementary DNA (cDNA ...
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bioRxiv - Immunology 2022Quote: ... and 0.5 units of Takara LA-Taq™ DNA polymerase (Takara Bio Inc., Kusatsu, Shiga, Japan). PCR cycle was 95°C for 4 minutes ...
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bioRxiv - Immunology 2022Quote: ... RNA was reverse transcribed using the SMART-Seq v4 ultra low input RNA kit (Takara Bio). cDNA quality was analysed on a 2100 Bioanalyser (Agilent) ...
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bioRxiv - Genomics 2022Quote: ... and 0.06 U/μl ribonuclease (RNase) inhibitor (2313A, Takara Bio ...
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bioRxiv - Genomics 2022Quote: RNA sequencing were prepared using the SMARTer® Stranded Total RNA-Seq Kit v3 -Pico Input Mammalian (Takara cat# 634485) with 5 µl RNA from either the total RNA or the ElementZero eluate as starting material according to the manufacturer’s protocoll ...
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bioRxiv - Genomics 2022Quote: ... cDNA was obtained using PrimeScript Reverse Transcriptase (Catalog No. 2690A, Takara, Japan) using a DNA elimination process.
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The arms race in noncoding regions of transposable element: the evolution of anti-silencing and RNAibioRxiv - Genetics 2022Quote: ... cDNA was synthesized using the PrimeScript III RT-PCR Kit (TaKaRa). Real-time qPCR (Figure 6A ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The target genomic region was amplified with PrimeSTAR GXL DNA Polymerase (Takara Bio) using a set of primers (Supplementary Table 1) ...
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bioRxiv - Genetics 2022Quote: ... on RetroNectin-coated plates (10 μg/cm2, Takara Bio) for at least 2 h ...
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bioRxiv - Genetics 2022Quote: ... on RetroNectin-coated plates (10 μg/cm2, Takara Bio). Cells were then transduced in the same medium ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA amplification was performed by adding 15 μL of Seq amp PCR mixture (12.5 μL of 2x SeqAmp buffer (Takara Bio), 0.05 μL of 100 μM N-IS PCR primer ...
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bioRxiv - Developmental Biology 2022Quote: A Smart-seq v4 3’ DE Kit (Takara Bio) was adapted to DRaqL as follows ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by the addition of 2.8 μL quenching buffer (0.7 μL of Triton X-100 [Nacalai Tesque], 0.7 μL of 2% bovine serum albumin [BSA] [Takara Bio]) and incubation at 70°C for 90 sec to quench the denaturing effect of SDc ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.5 μL of SeqAmp DNA polymerase (Takara Bio), and 1.95 μL of DDW ...