Labshake search
Citations for Takara Bio :
9901 - 9950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2021Quote: ... RT-qPCR reactions were performed in triplicate with TaqMan Master Mix: Premix Ex Taq (TaKaRa, Otsu, Japan) in a QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems ...
-
bioRxiv - Genetics 2021Quote: ... Reverse transcription was performed using PrimeScript II 1st strand cDNA Synthesis Kit (TAKARA) following the manufacture’s protocol ...
-
bioRxiv - Genetics 2021Quote: ... was amplified from genomic DNA isolated from tail and peripheral blood using 1 µl of prepped DNA in 20 µl of PCR reaction containing 0.4 µl of PrimerStar GXL (TAKARA Bio, R050A), 4 µl of 5× Buffer ...
-
bioRxiv - Genetics 2021Quote: ... The volume was brought to 11.5 μl and libraries were processed using the SMART-Seq HT library preparation kit (634456, Takara Bio) as described (Blackburn et al. ...
-
bioRxiv - Genetics 2021Quote: ... For all amplification steps we used PrimeStar GxL polymerase (Takara). Prior to injection ...
-
bioRxiv - Genetics 2021Quote: ... InFusion cloning (Takara) was used to replace the reverse transcription template portion of the 3’-extension with the BsaI-flanked red-fluorescent protein cassette from the original pU6-pegRNA-GG-acceptor plasmid ...
-
bioRxiv - Genetics 2021Quote: ... Table 1) against two unique sequences in the spCas9 that differs from dCas9GCN4 using Xfect RNA transfection reagent (Takara #631450) according to manufacturer instructions ...
-
bioRxiv - Genetics 2021Quote: ... pPB_U6::gRNA_EF1a::BFP-Puro containing a gRNA against Esg1 and pPY_CAG_Pbase using Xfect mESC transfection reagent (Takara #631320) and selection (Neomycin (300 μg/ml ...
-
bioRxiv - Genetics 2021Quote: ... The assembly reaction was then transformed into Stellar competent cells (Takara), and following an hour-long outgrowth ...
-
bioRxiv - Genetics 2021Quote: ... Libraries were prepared using SMARTer ThruPLEX DNA-Seq Kit (Takara Bio # R400675)
-
bioRxiv - Genetics 2021Quote: ... (ii) ectoderm differentiation was induced with NDiff (NB27 Takara #y40002) supplemented with 0,25 μM Retinoic Acid (Sigma Aldrich#R2625) ...
-
bioRxiv - Genetics 2021Quote: ... and were routinely cultured on gelatin-coated plates in t2i/L media: NDiff (N2B27) (Takara #y40002) supplemented with titrated 2i (0.2μM PD0325901 ...
-
bioRxiv - Genetics 2021Quote: ... by homology arm recombination using In-fusion HD-Cloning (Takara #639650) according to manufacturer’s instructions (Fig ...
-
bioRxiv - Genetics 2021Quote: ... The clarified lysate was subjected to Talon Metal Affinity column (Takara, Mountain View, CA) as per the manufacturer’s protocol ...
-
bioRxiv - Genetics 2021Quote: 1:500 mouse anti-GFP (Takara, JL-8)
-
bioRxiv - Genetics 2021Quote: We followed procedures and used controls from the Matchmaker Gold Yeast Two-Hybrid System from Takara Bio ...
-
bioRxiv - Microbiology 2021Quote: ... using the two-step In-Fusion strategy (Clontech, TaKaRa). First ...
-
bioRxiv - Microbiology 2021Quote: ... The TB Green™Premix Ex Taq™(TaKaRa) was used with a Thermal Cycler Dice Real Time System TP800 (TaKaRa ...
-
bioRxiv - Developmental Biology 2021Quote: Full length human COG4 was cloned into pCS2+ vector from plasmid hCOG4-siR-3myc in AAZ6 (Gift from Professor Vladimir V. Lupashin) using In-Fusion® HD Cloning Kit (TaKaRa Bio, 638909) with primers 5’-ATGGGAACCAAGATGGCGGA-3’ ...
-
bioRxiv - Developmental Biology 2021Quote: ... Nested PCR was performed using EpiTaq HS DNA Polymerase (TaKaRa R110A). The sequences of the PCR primer were designed with MethPrimer (http://www.urogene.org/cgi-bin/methprimer/methprimer.cgi ...
-
bioRxiv - Developmental Biology 2021Quote: ... Real Time (RT)-PCR was performed (SYBR Premix Ex TaqII, Takara Bio). Reference genes were Actin ...
-
bioRxiv - Genetics 2021Quote: ... Total RNA was reverse transcribed to cDNA using the PrimeScript cDNA synthesis kit (Takara Bio, Kusatsu, Japan). Quantitative PCR (qPCR ...
-
bioRxiv - Genetics 2021Quote: ... Illumina small RNA-Seq kits (Clontech, Mountain View, CA, USA) were then used to generate cDNA libraries ...
-
bioRxiv - Microbiology 2021Quote: ... using the two-step In-Fusion strategy (Clontech, TaKaRa). First ...
-
bioRxiv - Microbiology 2021Quote: ... pBICP35-CE1–CE8 transient expression vectors under the control of the 35S promoter were constructed using an In-Fusion system (Clontech, TaKaRa). The fragment containing the cDNA of CE1 was amplified with the primers 35S_CE1_Fw and 35S_CE1_Rv ...
-
bioRxiv - Genetics 2021Quote: Approximately 300-600 cells were collected from E6.5 embryos and directly transferred to 2.6 µl of Lysis Buffer (Takara Bio USA, Inc.) followed by snap-freezing at -80°C in preparation for cDNA synthesis using the SMART-Seq v4 assay ...
-
bioRxiv - Microbiology 2021Quote: ... was used with a Thermal Cycler Dice Real Time System TP800 (TaKaRa) for RT-qPCR ...
-
bioRxiv - Microbiology 2021Quote: ... pBICP35-CE1–CE8 transient expression vectors under the control of the 35S promoter were constructed using an In-Fusion system (Clontech, TaKaRa). The fragment containing the cDNA of CE1 was amplified with the primers 35S_CE1_Fw and 35S_CE1_Rv ...
-
bioRxiv - Genetics 2021Quote: ... TB Green™ Premix Ex Taq™ (Tli RNaseH Plus) (Takara bio RR820W) was used ...
-
bioRxiv - Genetics 2021Quote: ... Quantitative PCR was performed using TB Green™ Premix Ex Taq™ (Tli RNaseH Plus) (Takara bio RR820W) and a Quantstudio6 Flex Real Time PCR system (ThermoFisher ...
-
bioRxiv - Genetics 2021Quote: Using the SYBR Premix Ex TaqTM II kit (Takara Biotechnology, Tokyo, Japan) to perform the quantitative real-time PCR assay with CFX ConnectTM Real-Time PCR Detection System (Bio-Rad Laboratories ...
-
bioRxiv - Genetics 2021Quote: ... The cDNA samples were prepared from 1 μL total RNA per reaction using a reverse transcription kit (Takara Biotechnology, Tokyo, Japan) and stored at - 20°C for qRT-PCR assays ...
-
bioRxiv - Genetics 2021Quote: ... cDNA synthesis was performed using the RNA to cDNA EcoDry™ Premix (Random Hexamers) synthesis tubes (Clontech), heated at 42°C for 75 minutes ...
-
bioRxiv - Genetics 2021Quote: ... and a 1:5000 dilution of anti-GFP (Takara, 632381). Blots were washed (3 × 10 mins ...
-
bioRxiv - Genetics 2021Quote: Adult ovary total RNA was used to amplify the 5’ and 3’ UTR of Pxmeiw68 and PxnanosP using the SMARTer RACE 5’/3’ Kit (Takara, Japan). 5’ and 3’ regulatory sequences were then cloned from 4th instar larval gDNA ...
-
bioRxiv - Genetics 2021Quote: ... DsRed was detected by staining with rabbit anti-dsRed (Clontech 632496, 1:1000) followed by CyTM3-conjugated goat anti-rabbit (Jackson ImmunoResearch 111-165-144 ...
-
bioRxiv - Genetics 2021Quote: ... using SYBR® Premix Ex Taq™ (Perfect Real Time) (Takara). The primers (Telom F-Telom R ...
-
bioRxiv - Genetics 2021Quote: The PCR reaction mixture contained 0.05 μl Ex Taq polymerase (5 U/μl, TAKARA), 1μl 10X Ex Taq Buffer (20 mM ...
-
bioRxiv - Genetics 2021Quote: ... using SYBR® Premix Ex Taq™ (Perfect Real Time) (Takara). Reaction mixtures were incubated for 30 seconds (sec ...
-
bioRxiv - Genetics 2021Quote: ... Colony PCR was performed using Takara Taq polymerase (Clontech, TAKR001C). Plasmid DNA was isolated from cultured bacteria using QIAprep Spin Miniprep Kit (Qiagen ...
-
bioRxiv - Genetics 2021Quote: ... followed by a final extension at 72 °C for 10 min by using Takara Taq polymerase (Clontech, #TAKR001). To remove the DsRed cassette ...
-
bioRxiv - Genetics 2021Quote: ... and the DNA sequence of the modified locus was amplified by Prime STAR (Takara). The sequence was examined by Sanger sequencing using a commercial service (FASMAC).
-
bioRxiv - Genetics 2021Quote: ... Plasmids were constructed using DNA ligase (T4 DNA Ligase, Takara) or by ExoIII cloning [27] and introduced into the E ...
-
bioRxiv - Genetics 2021Quote: ... Lenti-X™ 293T Cells (Takara) were seeded on 10 cm dishes (6 × 106 cells per dish) ...
-
bioRxiv - Developmental Biology 2021Quote: ... Steps were performed on an automated system (Apollo 342, Wafergen Biosystems/Takara). After a final PCR amplification step ...
-
bioRxiv - Developmental Biology 2021Quote: ... Then, they were cloned into pmirGLO vector (Wuhan GeneCreate Biological Engineering Co., Ltd., Wuhan, China) between NheI and XhoI (TaKaRa, Nojihigashi, Japan), and the empty plasmid was transfected as the control group ...
-
bioRxiv - Developmental Biology 2021Quote: ... Real-time quantitative PCR was performed in triplicate using the SYBR Premix Ex Taq (Tli RNase H Plus) kit (Takara). The expression of TBP was used for the normalization of mRNA expression ...
-
bioRxiv - Developmental Biology 2021Quote: ... and reverse-transcription (RT) was performed with 1.5 µg of total RNA and PrimeScript reverse transcriptase (Takara Bio Inc, Kusatsu, Japan). RT-quantitative (q)-PCR was performed on a CFX96 C1000 real-time PCR cycler (Bio-Rad Laboratories ...
-
bioRxiv - Developmental Biology 2021Quote: ... and 3⍰×⍰105 cells were reverse transfected with 2μg of UniSAM DNA using the Xfect Transfection reagent (Clontech) and plated into a coated 6 well plate ...
-
bioRxiv - Developmental Biology 2021Quote: ... Plasmid construction was performed using Infusion cloning enzymes (Takara Bio, Mountain View, CA).