Labshake search
Citations for Takara Bio :
9851 - 9900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2021Quote: ... cDNA was obtained from 1 µg of total RNA from each sample (Super M-MLV reverse transcriptase, TAKARA) and QPCR was performed using TaqMan™ Gene Expression Master Mix (4369016 ...
-
bioRxiv - Cancer Biology 2021Quote: Total RNA was extracted from cells and quantified using RNA Isolation Reagent kit (9109, Takara, Tokyo, Japan). cDNA was obtained from 1 µg of total RNA from each sample (Super M-MLV reverse transcriptase ...
-
bioRxiv - Cancer Biology 2021Quote: ... and the oligonucleotides were inserted into C terminal of ARL4C-EGFP or ARL4C-FLAG-HA using In-Fusion HD Cloning Kit (Takara Bio Inc.).
-
bioRxiv - Cancer Biology 2021Quote: ... PCR amplified fragments and linearized vector by restriction enzyme digestion were assembled using In-Fusion HD Cloning Kit (Takara Bio Inc.). pEGFPN3-ARL4C was constructed as previously described(Matsumoto et al ...
-
bioRxiv - Cancer Biology 2021Quote: ... site-directed mutagenesis method was performed using PrimeSTAR Max DNA Polymerase (Takara Bio Inc., Shiga, Japan). To generate plasmid DNA with insertions ...
-
bioRxiv - Developmental Biology 2021Quote: ... Lentiviral supernatant was harvested at 48 h and 54 h after transfection and concentrated using the lenti-X concentrator (Clontech) at 4°C for 12-36 h ...
-
bioRxiv - Developmental Biology 2021Quote: ... PCR templates were generated by PCR reaction with biotinylated primers using high fidelity ClonAmp HiFi PCR mix (Takara Inc.). A mixture of Cas9-mSA mRNA (75ng/μl) ...
-
bioRxiv - Developmental Biology 2021Quote: ... concentrated using Lenti X concentrator (Clontech), and added to H9 hESCs growing on Matrigel along with 4 µg/ml polybrene ...
-
bioRxiv - Developmental Biology 2021Quote: All buffers were supplemented with RNAse inhibitors (Takara). 25mg of frozen postmortem human brain tissue was homogenized in cold lysis buffer (0.32M Sucrose ...
-
bioRxiv - Developmental Biology 2021Quote: Cells were lysed and RNA was reverse transcribed and amplified according to the SMARTer Ultra Low RNA Kit for Illumina Sequencing (version 1, Clontech). Libraries were prepared from cDNA using the NEBNext Ultra DNA library preparation for Illumina sequencing with indexed adaptors (New England Biolabs) ...
-
bioRxiv - Developmental Biology 2021Quote: Two-hybrid screening and assays were performed as described in the Matchmaker™ Pretransformed Libraries User Manual (Takara PT3183-1) using a Drosophila Mate & Plate™ Library - Universal Drosophila (Normalized A+ RNA isolated from embryo (~20 hr) ...
-
bioRxiv - Developmental Biology 2021Quote: ... Library preparations were done using SMARTer® Stranded RNA-Seq Kit (Clontech Laboratories, Inc. #634836 or #634837) according to manufacturer’s guidelines ...
-
bioRxiv - Immunology 2021Quote: ... and all resulting RNA was used as input for the SMART-seq v4 cDNA synthesis kit (Takara) with 12 cycles of PCR amplification ...
-
bioRxiv - Immunology 2021Quote: ... 1ng of total RNA was pre-amplified with the SMARTer Ultra Low Input kit v4 (Clontech, Mountain View, CA) per manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2021Quote: ... 5 pmol of probe and 10 μl of Premix Ex Taq (2×) (Takara). Positive amplification controls were DNA purified from ASFV virions at different concentrations used as standards ...
-
bioRxiv - Developmental Biology 2021Quote: ... cDNA was generated from purified total RNA using PrimeScript RT Master Mix (Takara Bio). PCR amplification of the sequence corresponding to EcI-2/AAEL007691 ...
-
bioRxiv - Immunology 2021Quote: ... or Western BLoT Ultra Sensitive HRP Substrate (Takara Bio).
-
bioRxiv - Immunology 2021Quote: ... The mutated NLRP3-D303N and NLRP3-L353P were generated using the PrimeSTAR Mutagenesis Basal kit (Takara Bio, Shiga, Japan) (Aizawa et al. ...
-
bioRxiv - Immunology 2021Quote: ... LentiX293T cells were obtained from TAKARA (Takara Bio, Shiga, Japan) and cultured in DMEM supplemented with 10% FCS ...
-
bioRxiv - Immunology 2021Quote: ... immunoreactive bands were visualized using Western BLoT Quant HRP Substrate (Takara Bio) or Western BLoT Ultra Sensitive HRP Substrate (Takara Bio).
-
bioRxiv - Immunology 2021Quote: ... Real-time PCR was performed using SYBR Premix Ex Taq II (Takara Bio). The primers used in the assay were as follows ...
-
bioRxiv - Immunology 2021Quote: ... LentiX293T cells were obtained from TAKARA (Takara Bio, Shiga, Japan) and cultured in DMEM supplemented with 10% FCS ...
-
bioRxiv - Immunology 2021Quote: ... pLVX-IRES-mCherry-mDDX18-S354L were generated by cloning the cDNA of mDDX18 or its mutants into the lentiviral vector pLVX-IRES-mCherry (TaKaRa, Japan). To package the recombinant lentiviruses ...
-
bioRxiv - Immunology 2021Quote: ... The first-strand cDNA synthesis was performed with the Prime Script RT Master Mix kit (Takara Bio Europe, France). Quantitative real-time PCR was performed at 60°C using Takyon ROX SYBR MasterMix (Eurogentec ...
-
bioRxiv - Immunology 2021Quote: ... cDNA was synthesized and amplified directly from intact cells using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio USA) according to the manufacturer protocol ...
-
bioRxiv - Microbiology 2021Quote: ... Clarified lysates were loaded onto a 1 mL TALON (Takara) column ...
-
bioRxiv - Immunology 2021Quote: ... DNase-treated total RNA was used to prepare cDNA libraries with the SMARTer smRNA-Seq Kit (Takara Bio, Mountain View, CA). Libraries were sequenced as 50 bp single-end reads on an Illumina HiSeq sequencer ...
-
bioRxiv - Immunology 2021Quote: ... Protein eluted from the Strep-Tactin column was then applied to pre-equilibrated TALON Metal Affinity Resin (Takara) and allowed to enter the column by gravity flow ...
-
bioRxiv - Immunology 2021Quote: ... pGAE-UFO.750 or pConSgp160 using the CalPhos™ Mammalian Transfection Kit (Clontech Laboratories) or transduced with 5 MOI of LV-UFO.664 or LV-UFO.750. ...
-
bioRxiv - Immunology 2021Quote: ... and stimulated the next day for 24h with the appropriate chemicals (doxycycline, Sigma D3072; dimerizer, Clontech 635059; (5Z)-7-Oxozeaenol / TAK1i ...
-
bioRxiv - Immunology 2021Quote: ... 24-well non-tissue culture plate wells were pre-coated with RetroNectin® (Takara Bio USA, Inc.) according to manufacturer’s instructions and then day 10 cultured Tregs were added at 0.3 x 106 cells/well in 0.3mL of cell culture media ...
-
bioRxiv - Immunology 2021Quote: ... 0.25 μL Recombinant RNase Inhibitor (Clontech), 2 μL Betaine (5 M Sigma) ...
-
bioRxiv - Immunology 2021Quote: ... 0.5 μL SMARTScribe™ Reverse Transcriptase (Clontech), 2 μL 5x First Strand buffer ...
-
bioRxiv - Immunology 2021Quote: ... 2.5 mM dNTP and 2 U/μL of recombinant RNase inhibitor (Clontech) then spun down and frozen at –80 °C.
-
bioRxiv - Immunology 2021Quote: ... The mix SYBR Green Premix ExTaq (Takara) was used for quantitative PCR in an iCyclerMyiQ thermocycler (BioRad) ...
-
bioRxiv - Immunology 2021Quote: ... and PrimeScript™ RT Reagent Kit (RR037B, Takara Bio, Saint-Germaine-en-Laye, France), respectively ...
-
bioRxiv - Immunology 2021Quote: ... using the Reverse Transcriptase Kit (Takara, Japan). Quantitative PCR reactions were carried out in duplicate ...
-
bioRxiv - Immunology 2021Quote: ... SYBR green master mix (Takara, Japan) was used to for two-step qPCR assay after cDNA synthesis using PrimeScript® reverse transcriptase kit (Takara ...
-
bioRxiv - Immunology 2021Quote: ... was used to for two-step qPCR assay after cDNA synthesis using PrimeScript® reverse transcriptase kit (Takara, Japan). Gene expression was normalized to the expression level of β-actin ...
-
bioRxiv - Immunology 2021Quote: ... Such isolated naïve CD8 T cells were cultured for 3d prior transducton with 3rd generation lentiviral vectors on RetroNectin (Takara) coated cell culture plates (according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2021Quote: ... and F1 and F2 arms homologous to upstream and downstream sequences of the A41L ORF using in-fusion cloning (Takara Bio). The resultant spike transfer cassette was linearized by NotI and transfected into the MCL for homologous recombination with SCV ...
-
bioRxiv - Immunology 2021Quote: ... pECFP-N1 (Clontech) was subcloned into the YFP-SHP-1 expression vector to obtain the chimeric protein YFP-SHP-1-CFP ...
-
bioRxiv - Immunology 2021Quote: ... The construct was sequenced and the NF-κB reporter cassette was transferred into pSIRΔ vector (Clontech) using XhoI and NotI ...
-
bioRxiv - Genetics 2021Quote: Total RNA was extracted from wing discs of individual silkworms at the wandering stage using the MicroElute Total RNA Kit (OMEGA) and reverse transcription was performed using the PrimeScript RT reagent Kit (Takara). qRT-PCR experiments were performed using Hieff SYBR Green Master Mix (YEASEN) ...
-
bioRxiv - Genetics 2021Quote: ... and reverse transcription was performed using the PrimeScript RT Reagent Kit (Takara). Equal masses (concentration times volume ...
-
bioRxiv - Genetics 2021Quote: ... Strand specific RNA-seq libraries were then constructed using the SMARTer Stranded RNA-Seq HT Kit (Clontech #634839), according to the manufacturer’s protocol ...
-
bioRxiv - Genetics 2021Quote: Yeast Matchmaker Two-Hybrid System was obtained from Clontech (now Takara Bio), including pGBKT7 bait vector ...
-
bioRxiv - Genetics 2021Quote: Yeast Matchmaker Two-Hybrid System was obtained from Clontech (now Takara Bio), including pGBKT7 bait vector ...
-
bioRxiv - Genetics 2021Quote: ... minimal SD base media (Takara Bio, 630411), and a composition of every essential amino acid except tryptophan (SD/-Trp ...
-
bioRxiv - Genetics 2021Quote: ... Real-time PCR was performed using TB Green Premix Ex Taq II (Tli RNaseH Plus) (TAKARA) and ViiA7 Real-Time PCR system ...