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Citations for Takara Bio :
8001 - 8050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... gDNA from all cells were isolated using the Machery Nagel L Midi NucleoSpin Blood Kit (Clontech, 740954.20). Modifications to the manufacturer’s instructions were added as follows ...
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bioRxiv - Cancer Biology 2022Quote: The expression vectors used in the study were initially derived from pEGFP-N1 and pEGFP-C1 (Takara, La Jolla, CA). A double stranded oligomer encoding StrepTag II affinity tag was cloned ...
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bioRxiv - Immunology 2022Quote: ... and RNA quality (Bioanalyzer) were measured again before proceeding with cDNA libraries construction using the SMARTseq v4 Ultra-Low Input kit (Clontech).
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bioRxiv - Physiology 2022Quote: ... to 500μl using Lenti-X Concentrator (Clontech, Mountain View, CA), and 100μl of concentrated virus was used to infect cells ...
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bioRxiv - Cancer Biology 2022Quote: ... Lentivirus was produced by transfecting Lenti-X 293T cells (Clontech) with the SMARTvector transfer plasmid and packaging/pseudotyping plasmids ...
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bioRxiv - Genomics 2022Quote: ... 0.5 μg of total RNA from each sample was used to synthesize cDNAs from polyA+ RNA using Clontech SMART-Seq® v4 Ultra® Low Input RNA Kit for the Fluidigm® C1™ System (Clontech, Cat. No. 635026) following the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2022Quote: ... The extracted RNA was reverse transcribed using PrimeScript RT Master Mix (Takara) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... human CCDC22 was first cloned into pEGFP-C1 (Clontech) between Sal1 and BamH1 ...
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bioRxiv - Developmental Biology 2022Quote: 1 µg of tissue specific (ovary) RNA was used for First strand cDNA synthesis using PrimeScriptTM 1st strand cDNA Synthesis Kit (Takara, Cat# 6110A) using the following program ...
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bioRxiv - Cell Biology 2022Quote: ... The Lenti-XTM HTX Packaging System (Clontech, Saint-Germain-en-Laye, France) was used with the appropriate plasmid (generated by DC Bioscience ...
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bioRxiv - Immunology 2022Quote: ... The signal was detected using the Western Blot Ultra-Sensitive HRP Substrate (Takara) and imaged using the Fusion FX Imaging system (PeqLab Biotechnologie).
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bioRxiv - Evolutionary Biology 2022Quote: ... with 0.1 µL of Taq Polymerase 5 U.µL-1 (TaKaRa Ex Taq® Kit MgCl2 Free Buffer) (TaKaRa Ex Taq ...
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bioRxiv - Evolutionary Biology 2022Quote: ... with 0.1 µL of Taq Polymerase 5 U.µL-1 (TaKaRa Ex Taq® Kit MgCl2 Free Buffer) (TaKaRa Ex Taq, TaKaRa Bio, Shiga, Japan), 2.5 µL PCR Buffer 10X ...
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bioRxiv - Evolutionary Biology 2022Quote: ... with 0.1 µL of Taq Polymerase 5 U.µL-1 (TaKaRa Ex Taq® Kit MgCl2 Free Buffer) (TaKaRa Ex Taq, TaKaRa Bio, Shiga, Japan), 2.5 µL PCR Buffer 10X ...
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bioRxiv - Neuroscience 2022Quote: ... and rabbit anti-dsRed (1:1000; Takara). The following corresponding Alexa Fluor™ conjugated secondary antibodies (1:750 ...
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bioRxiv - Neuroscience 2022Quote: ... that were filled with 2μl of picking buffer [1μL RNAse inhibitor (Takara #2313A) in 49 μL PBS] ...
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bioRxiv - Immunology 2022Quote: ... The rescued rAds were purified using Adeno-X Maxi Purification Kit (Cat. No: 631533, Takara, Japan).
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bioRxiv - Immunology 2022Quote: ... by PCR and subcloned in pEGBacMam using In-Fusion seamless DNA cloning (Takara Bio). PCR was used to generate the pEGBacMam-Strep-FLAG-Strep-EROS construct ...
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bioRxiv - Immunology 2022Quote: ... a kind gift from Eric Gouaux (Oregon Health & Science University; (Goehring et al., 2014)) using In- Fusion seamless DNA cloning (Takara Bio). The cDNAs encoding for human NOX5 (isoform b ...
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bioRxiv - Genomics 2022Quote: ... cDNAs from low yield polyA+ RNA was synthesized using SMART-Seq® v4 Ultra® Low Input RNA Kit for the Fluidigm® C1™ System (Clontech, Cat. No. 635026), SMARTer-seq® chemistry (Switching Mechanism at 5’ End of RNA Template and Locked Nucleic acid technology) ...
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bioRxiv - Plant Biology 2022Quote: ... and TB Green® Premix EX Taq·II SYBR Green dye was used (TaKaRa, www.takarabio.com). For internal control ...
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bioRxiv - Microbiology 2022Quote: ... Purified RNA product was immediately used with the One-step PrimeScript RT-PCR Kit (Takara). Primers and probes were obtained from IDT ...
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bioRxiv - Cell Biology 2022Quote: ... H2B-mCherry organoids were infected with LV.EF1.AcGFP1-Mem-9 lentivirus particle (Clontech, Takara Bio USA). For the H2B-miRFP670 line ...
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bioRxiv - Genomics 2022Quote: ... PCR was performed using Taq polymerase (TAKR001C, ClonTech) when running DNA fragments on a gel ...
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bioRxiv - Cell Biology 2022Quote: ... H2B-mCherry organoids were infected with LV.EF1.AcGFP1-Mem-9 lentivirus particle (Clontech, Takara Bio USA). For the H2B-miRFP670 line ...
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bioRxiv - Genetics 2022Quote: ... The extracted and DNase I (TaKaRa, Japan) treated RNA was sent to Macrogen Japan Corporation for library preparation by TruSeq stranded mRNA (paired-end ...
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bioRxiv - Microbiology 2022Quote: ... ORF3a was amplified with Takara PrimerSTAR PCR enzyme (R050B, Takara) using the following primers:
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bioRxiv - Cell Biology 2022Quote: ... Viral particles were generated using Lenti-X 293T cells (Takara; 632180) cultured in DMEM with 10% heat inactivated fetal bovine serum (MilliporeSigma ...
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bioRxiv - Cell Biology 2022Quote: ... Collected lentivirus was tittered using Lenti-X™ GoStix™ Plus (Takara;631280). Lentiviral transduction was initiated at the first day of Stage 6 with an MOI of 5 for 24 hr.
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bioRxiv - Cell Biology 2022Quote: ... Viral containing supernatant was collected at 96 hr post transfection and concentrated using Lenti-X concentrator (Takara; 631232). Collected lentivirus was tittered using Lenti-X™ GoStix™ Plus (Takara;631280) ...
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bioRxiv - Genetics 2022Quote: ... Cas9 was amplified from the expression plasmid using CloneAmp HiFi PCR Premix (Takara Bio) for 35 cycles (98□ for 10 s ...
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bioRxiv - Biophysics 2022Quote: ... and purified using TALON metal affinity resin (Clontech).
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bioRxiv - Genetics 2022Quote: ... DNA samples were then amplified with PrimeSTAR GXL DNA Polymerase (Takara Bio) with PCR forward/reverse primers containing Illumina adapter sequences (Table S3 ...
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bioRxiv - Genetics 2022Quote: ... In-Fusion Cloning (Takara Bio) was used to clone PRDM9-Cas9 ...
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bioRxiv - Bioengineering 2022Quote: ... The sorted cells were returned to DMEM with TET-free FBS (Takara). MDA-MB-231-mARGAna was sorted twice ...
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bioRxiv - Bioengineering 2022Quote: ... with a TRE3G promoter (Takara Bio) for doxycycline-inducible expression.
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bioRxiv - Neuroscience 2022Quote: ... followed by the Low Input Library Prep Kit v2 (Takara #634899). We pooled up to 12 samples (with different barcodes ...
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bioRxiv - Neuroscience 2022Quote: ... We used the SMART-seq v4 ultra-low input RNA kit for sequencing (Takara #634897) using the manufacturer’s instructions for single-cell samples ...
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bioRxiv - Neuroscience 2022Quote: ... The pEGFP-C1 plasmid is from Clontech (Addgene plasmid # 13031 ...
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bioRxiv - Molecular Biology 2022Quote: ... Aliquots of 2.0 ug of the total RNA were collected for cDNA synthesis by using Clontech Oligo dT cDNA synthesis kit (Takara Bio USA ...
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bioRxiv - Molecular Biology 2022Quote: ... the transfection medium was replaced with 2 ml of reduced serum culture medium (5% FCS) supplemented with 300 μM A/C heterodimerization agent (formerly AP21967, Takara BioInc), 20 mM HEPES and 10 μM cholesterol (balanced with methyl-β-cyclodextrin ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 ml of Lenti-X concentrator (Takara Bio) was added to 12 ml of cell supernatant and the mixture incubated at 4°C with gentle agitation for 18 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... serving as homology arms for directional cloning of the library into RD-seq plasmid (zfh1-DSCP-Gal4-DBD, Suppl. Table 2) vector using In-Fusion HD (Clontech 639650).
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bioRxiv - Molecular Biology 2022Quote: ... Cleared lysate was bound to 3 mL (=1.5 mL bed volume) Talon SuperFlow Metal Affinity Resin (TaKaRa) per protein preparation ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysate was cleared by centrifugation at 43 550×g and afterwards bound to 1 mL 50% slurry of Talon Metal Affinity Resin (TaKaRa) equilibrated in lysis buffer without PMSF ...
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bioRxiv - Molecular Biology 2022Quote: ... Tagged proteins were bound to 6 (=3 mL bed volume) or 3 mL (=1.5 mL bed volume) pre-equilibrated Talon SuperFlow Metal Affinity Resin from TaKaRa (LarA wt ...
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bioRxiv - Molecular Biology 2022Quote: ... Amplification of target fragments was performed by PrimeSTAR Max DNA Polymerase (Takara).
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bioRxiv - Molecular Biology 2022Quote: ... mEGFP and 3’UTR fragments were amplified by PrimeSTAR Max DNA polymerase (Takara). The replication origin and selection marker were derived from a mammalian expression vector ...
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bioRxiv - Molecular Biology 2022Quote: ... pCAGGS was digested by SpeI and BamHI and assembled with the PCR products by an In-Fusion HD cloning system (Clontech). After transformation into DH5α ...
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bioRxiv - Molecular Biology 2022Quote: ... and first strand cDNA prepared using the PrimeScript RT (Clontech) according to manufacturer’s instructions ...