Labshake search
Citations for Takara Bio :
7951 - 8000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... SYBR Premix Ex TaqII (Takara, Shiga, Japan) was used in the StepOnePlus Real-Time PCR thermocycler (Applied Biosystems ...
-
Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... Protein concentrations were assessed with the BCA protein assay kit (Takara BCA Protein Assay Kit ...
-
Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... according to the manufacturer’s instructions and reverse transcribed using the PrimeScript RT Reagent Kit with the gDNA Eraser (Takara, Shiga, Japan). To amplify cDNA ...
-
bioRxiv - Immunology 2022Quote: ... Cells were lysed by sonication and then purified via a TALON cobalt affinity resin (Clontech). The resin was washed with 40 mM Tris-HCl ...
-
bioRxiv - Immunology 2022Quote: ... Real-time PCR analysis was performed using the Probe qPCR Mix (Takara Bio, Otsu, Shiga, Japan) and Applied Biosystems 7500 Real-Time PCR System (Applied Biosystems ...
-
bioRxiv - Immunology 2022Quote: ... All constructs were purified from supernatants by passage over Co-NTA TALON resin (Takara) followed by size exclusion chromatography on a Superdex 200 Increase (GE Healthcare ...
-
bioRxiv - Immunology 2022Quote: ... cDNA was synthesized from DNase I-treated RNA using SMARTScribe™ Reverse Transcriptase (Clontech) at 42°C for 90 min followed by 70°C for 15 min with 0.2 μM each of gene-specific reverse primers (hIgG-RV1 ...
-
bioRxiv - Immunology 2022Quote: ... RNA concentrations were determined using the Qubit high-sensitivity RNA assay and small RNA libraries were created using the Clontech SMARTer smRNA-Seq preparation kit according to manufacturer instructions (Takara). Small RNA inserts were further enriched using AMPure bead selection (Beckman Coulter ...
-
bioRxiv - Immunology 2022Quote: ... Supernatants (50 μL) were then combined with an equivalent volume of LDH reaction buffer/substrate mix (Takara Bio Inc.) in a clear-bottom 96-well plate ...
-
bioRxiv - Immunology 2022Quote: ... and cDNAs were synthesized using a SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... using SYBR Premix Ex Taq II or TB Green Premix Ex Taq II (Takara Bio). The primer sequences used were shown in Table S5 (Tara et al. ...
-
bioRxiv - Immunology 2022Quote: ... or TB Green Premix Ex Taq II (TaKaRa Bio). All data are presented as relative expression levels normalized to Hprt expression ...
-
bioRxiv - Immunology 2022Quote: ChIP-seq libraries were prepared using a ThruPLEX DNA-seq Kit (Clontech) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... After the optimization of PCR cycle number using SYBER Green I Nucleic Acid gel Stain (Takara Bio), transposed fragments were amplified using NEBNext High Fidelity 2× PCR Master mix and index primers ...
-
bioRxiv - Cancer Biology 2022Quote: ... the Lenti-X™ 293T cell line (Takara, #632180) was used ...
-
bioRxiv - Cancer Biology 2022Quote: ... 2 µg of plasmids were diluted in 200 µL Xfect transfection reagent (631317, Takara). The mixture was incubated at room temperature for 15 min ...
-
bioRxiv - Cancer Biology 2022Quote: The parental MCF10A cells were stably transduced with tetracycline-OFF-transactivator system (tet-OFF; Clontech) following a previously described protocol (pubmed.ncbi.nlm.nih.gov/27815877/) ...
-
bioRxiv - Cancer Biology 2022Quote: ... The biosensor expression cassette was transduced into the stable tet-OFF MCF10A cell line under the regulation of a tet-inducible minimal CMV promoter (pRetro-X-Puro; Clontech) and selected using puromycin for a stably integrated population ...
-
bioRxiv - Cancer Biology 2022Quote: ... qPCR was performed using with the SYBR Premix Ex Taq mix (TaKaRa) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... cDNA synthesis and library preparation were performed in the UNMCCC ATG Shared Resource using the SMARTer Universal Low Input RNA Kit for Sequencing (Clontech, San Jose, CA) and the Ion Plus Fragment Library Kit (Life Technologies ...
-
bioRxiv - Cancer Biology 2022Quote: ... then cDNA was synthesized without RNA purification using SMART-seq HT (Takara). For the PANC-1 cells ...
-
bioRxiv - Cancer Biology 2022Quote: ... and 1 ng of RNA was used for the reverse transcription reaction using SMART-seq HT (Takara, 634455). For the PDO xenograft ...
-
bioRxiv - Cancer Biology 2022Quote: ... was subcloned by PCR into Xho1/BamH1 restriction sites of lentiviral expression vector pLVX-puro (Clontech), using 5’ region primer ...
-
bioRxiv - Cancer Biology 2022Quote: ... was subcloned by PCR into Xho1/BamH1 restriction sites of lentiviral expression vector pLVX-puro (Clontech), using 5’ region primer ...
-
bioRxiv - Cell Biology 2022Quote: ... was obtained from Clontech and knocked-in at the X-linked hypoxanthine guanine phosphoribosyl transferase (Hprt ...
-
bioRxiv - Cell Biology 2022Quote: ... mouse α-GFP JL-8 (cat. no. 632381, Takara) used at a concentration of 1:2,000 ...
-
bioRxiv - Cell Biology 2022Quote: ... The reverse transcription step was performed using Takara’s PrimeScriptTM RT Master Mix (RR036A; Takara, Japan). A brilliant SYBR green PCR master mix (4913914 ...
-
bioRxiv - Cell Biology 2022Quote: ... The yeast strain AH109 was transformed with the constructs in destination vectors using lithium acetate transformation (Yeast Protocols Handbook PT3024-1, Clontech). The transformants were grown on synthetic defined medium (0.67% yeast nitrogen base ...
-
bioRxiv - Cell Biology 2022Quote: ... All above sequences along with MBNL1 and MBNL2 isoforms imaged in primary neurons were cloned downstream of GFP in a pEGFP-C1 plasmid backbone under control of a CMV promoter (Takara). Full-length kinesin sequences were amplified from mouse cDNA and inserted upstream of an mScarlet-containing plasmid in a pcDNA3.1 backbone under the control of CMV promoter.
-
bioRxiv - Cell Biology 2022Quote: All plasmid fusions assembled in-house were generated through standard ligation-based methods using the In-Fusion cloning kit according to manufacturer protocols (Takara, 638920). MBNL1 RNA-interaction and chelation mutant sequences were designed based on plasmids generated by Andrew Berglund (Purcell et al. ...
-
bioRxiv - Cell Biology 2022Quote: ... Donor vectors containing the GFP or mKate2 coding sequence flanked by 1kb homology arms adopted from both 3′ and 5′ sides of the insertion site were generated by cloning of PCR-amplified tags and arms into linearized pGEM-3z vector by In-Fusion HD Cloning Kit (Takara Bio). Obtained gRNA expression plasmids and donor plasmids were injected into the y w embryos with a final concentration of 120 ng/ul for each ...
-
bioRxiv - Cell Biology 2022Quote: ... the lentivirus was collected and concentrated using Lenti-XTM concentrator per manufacturer instructions (Clontech). Following concentration ...
-
bioRxiv - Cell Biology 2022Quote: ... We used IF cloning for the pBI dual expression vector containing a minimal CMV promoter (Takara Bio USA) (Fig ...
-
bioRxiv - Cell Biology 2022Quote: ... Constructs coding for FRB (DmrA) and FKBP (DmrC) sequences were obtained from ARIAD Pharmaceuticals and are now available from Takara Bio Inc ...
-
bioRxiv - Developmental Biology 2022Quote: ... 293T cells (Takara Bioscience, 632180) were then transfected using lenti-X shots following the manufacturer’s protocol (Clonetech ...
-
bioRxiv - Developmental Biology 2022Quote: ... rabibit anti-Tomato (Clontech 632496 1:500), mouse anti-Ebf3 (Abnova H00253738-M05 1:1000) ...
-
bioRxiv - Developmental Biology 2022Quote: Rapid amplification of cDNA end (RACE) was performed using the SMARTer® RACE 5’/3’ Kit (Takara, #634858). 1ug of freshly isolated RNA from E8.5 embryo hearts was used to generate first strand cDNA according to the manufactural protocol ...
-
bioRxiv - Developmental Biology 2022Quote: ... Co-IPs were carried out by incubating the samples with 30 μL of protein A agarose bead slurry for 4h at 4°C in a rotating wheel and with anti-mCherry (Takara 632496) of 1:1000 dilution ...
-
bioRxiv - Developmental Biology 2022Quote: 293T cells (Takara Bioscience, 632180) were cultured in DMEM (Thermo Fisher ...
-
bioRxiv - Cell Biology 2022Quote: HeLa Tet-on cells were obtained from Takara Bio ...
-
bioRxiv - Cell Biology 2022Quote: ... then imaged after overnight expression and ∼3 hour incubation with 500 nM A/C heterodimerizer linker drug (Takara, 635055) or 100% ethanol ...
-
bioRxiv - Cell Biology 2022Quote: ... pAc-GFP-C1 vector was purchased from Clontech. LAMP1-KillerRed was subcloned from LAMP1-RFP ...
-
bioRxiv - Cell Biology 2022Quote: We used the In-Fusion™ (IF) HD cloning system online primer design website (Takara Bio USA, Mountain View, CA) to create primer sets (Table S1 ...
-
bioRxiv - Microbiology 2022Quote: ... A 5 µl aliquot was removed from each sample for immunoblots using mouse anti-GFP (1:5,000, Clontech #632381) to detect A3-EGFP ...
-
bioRxiv - Microbiology 2022Quote: ... Input and elution samples were analyzed by immunoblot using mouse anti-GFP (1:5,000, Clontech #632381) to detect A3-EGFP ...
-
bioRxiv - Molecular Biology 2022Quote: ... qPCR was performed in triplicate with TB green Premix Ex Taq from Takara and the Biorad CFX thermocycler ...
-
bioRxiv - Neuroscience 2022Quote: ... The pHluorin-SYT9 cassette replaced GFP in the cloning site of the FSGW vector via In-Fusion Cloning (Takara Bio, San Jose, CA, USA). The SP-pH construct was generated by PCR SOE and comprised ...
-
bioRxiv - Neuroscience 2022Quote: All cloning was accomplished using the In-Fusion HD cloning kit (Takara). To create the BioID2 fusion constructs ...
-
bioRxiv - Neuroscience 2022Quote: ... were purchased from Addgene and Genscript or amplified from human adult and fetal brain RNA (Takara) (see Table S10)(Alford et al. ...
-
bioRxiv - Neuroscience 2022Quote: All viruses were made using the 2nd generation lentiviral packaging systems in Lenti-X HEK293 FTT cells (Takara). Lenti-X cells were passaged maximum 3 times before being used for virus production in HEK media (High glucose DMEM with 4 mM GlutaMAX ...