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Citations for Takara Bio :
7801 - 7850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... After removal of the blocking solution 100 μL of anti-GFP (Living Colors GFP monoclonal antibody, Clontech) diluted 1:10,000 in antibody solution (1x PBS ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli cells: Stellar competent cells (Takara Bio, Cat#636763), chemically competent DH5α cells or NEB 10-beta competent cells (NEB ...
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bioRxiv - Microbiology 2022Quote: The NiCE chip was run using the SmartChip MyDesign chip (Takara Bio) and the SmartChip Real-Time PCR system (Takara Bio) ...
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bioRxiv - Microbiology 2022Quote: ... The PCR product was ligated into the NotI site of the pNEN142 vector (Minetoki et al., 2003) with an In-Fusion HD Cloning Kit (Takara Bio, Kusatsu, Japan); this vector contains the improved promoter of the A ...
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bioRxiv - Microbiology 2022Quote: ... performing PCR reactions with TaKaRa Ex Taq and reagents (Takara Bio, Japan). Successful results were confirmed by bidirectional Sanger sequencing performed by GATC Biotech (Germany).
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bioRxiv - Molecular Biology 2022Quote: ... GenTLE (from Yeast) High Recovery Kit (9082, Takara Bio). The genomic DNA concentration was quantified using the Quantifluor® ONE dsDNA Dye System (E4971 ...
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bioRxiv - Molecular Biology 2022Quote: ... genes were cloned into a His-SUMO fusion protein expression vector (Kim et al, 2018) using an In-Fusion Cloning Kit (639648, Takara Bio). The glycine-arginine-proline (GRP ...
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bioRxiv - Molecular Biology 2022Quote: ... qRT-PCR was perfor med using a qPCRsoft (Analytik Jena AG, Germany) with a SYBR®Green PC R Master Mix Kit (Takara Bio), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: 7.5 ng cDNA were mixed with 2X TB green Premix Ex Taq II (Takara, RR820B), 10μM forward and reverse primers ...
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bioRxiv - Microbiology 2022Quote: ... A total of 500 ng RNA was reverse transcribed into complementary DNA (cDNA) using a PrimeScript II 1st Strand cDNA Synthesis kit (TaKaRa) or a QuantiNova Reverse Transcript Kit (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... the first round of PCR amplification was performed using Takara Ex Taq® Hot-Start Version (RR006A; TaKaRa Bio, Shiga, Japan). The amplification comprised 40 cycles of denaturation at 98 °C for 10 s ...
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bioRxiv - Molecular Biology 2022Quote: ... the GST gene from the pEGKG plasmid (29) was amplified by PCR and inserted immediately upstream of GFPS65T using the In-Fusion HD cloning kit (Takara).
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bioRxiv - Microbiology 2022Quote: ... Release of LDH into the culture supernatant was quantified after infection using an LDH Cytotoxicity Detection Kit (Clontech). LDH release was normalized to mock-infected cells and cells treated with 1% Triton to establish maximum LDH release ...
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bioRxiv - Bioengineering 2022Quote: ... the PCR products were ligated into the pLVX-TRE3G vector (Clontech, CA) and verified by DNA sequencing (Eton Bioscience ...
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bioRxiv - Cancer Biology 2022Quote: KPC cells were transduced with either an empty vector (EV) pLVX-IRES-mCherry (Clontech, Mountain View, CA) or the same backbone vector expressing the human K17 open reading frame cDNA ...
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bioRxiv - Bioengineering 2022Quote: The nanobody-SPOP constructs were cloned into the pEGFP-N3 vector (Clontech) using restriction enzymes BamHI ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from cells using the TRIzol RNA isolation reagents (Takara bio, Japan). The first strand cDNA was reverse transcribed from the RNA templates using the GoScript Reverse Transcription system kit (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... qPCR was performed in triplicates with the SYBR Premix Taq II master mix (Takara) and specific primers on the LightCycler 96 (Roche) ...
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bioRxiv - Cell Biology 2022Quote: ... Deletion and point mutation constructs were produced through inverse PCR using Takara In-Fusion HD cloning kit (#638920) (Takara Bio Inc., Kusatsu, Shiga, Japan). Primers used are listed in supplementary materials.
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bioRxiv - Cell Biology 2022Quote: ... using In-Fusion HD Cloning Plus (Takara Bio). Short Gly/Ser linkers were added between GFP and Eps8 ...
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bioRxiv - Plant Biology 2022Quote: ... and cDNA was synthesized with a PrimeScript cDNA Synthesis Kit (TaKaRa). Gene expression levels were quantified using gene-specific primers (Supplemental Table S2 ...
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bioRxiv - Physiology 2022Quote: ... After RNA Reverse transcription (Prime Script RT reagent, Takara), cDNA levels were measured by qPCR Fast SYBR™ Green Master Mix (Ref:4385618 ...
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bioRxiv - Physiology 2022Quote: ... and cDNA libraries were generated using SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara Bio, Inc., Kusatsu, Japan). Libraries were high-output single-end sequenced on a NextSeq500 instrument (Illumina ...
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bioRxiv - Plant Biology 2022Quote: ... Double-stranded DNAs corresponding to the guide RNA sequences were generated and inserted into AarI-digested pMpGE013 by ligation reaction using Mighty Mix (Takara Bio).
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bioRxiv - Molecular Biology 2022Quote: ... and Random Primer (nonadeoxyribonucleotide mix: pd(N)9) (TaKaRa) from the total RNA of Ophiocordyceps sp ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA fragments containing eIF4A iso4 were PCR-amplified and inserted into pColdI (TaKaRa) downstream of the His tag with In-Fusion HD (TaKaRa) ...
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bioRxiv - Neuroscience 2022Quote: ... mRNA 500 ng was used for cDNA synthesis in 10 μl volume according to the manufacturer’s instructions of PrimeScriptTM RT-PCR kit (Takara Bio Inc., Shiga, Japan). Real-time RT-PCR was then performed using the KAPA SYBR FAST qPCR Master Mix (2x ...
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Activity-dependent stabilization of nascent dendritic spines requires non-enzymatic CaMKIIα functionbioRxiv - Neuroscience 2022Quote: ... except 6-8 µg of DsRed-Express (Clontech) and 6 μg of mEGFP-tagged constructs or 5-10 μg of mEGFP were coated onto 6-7 mg of 1.6 μm gold beads ...
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bioRxiv - Cell Biology 2022Quote: ... and Foxa3 were obtained by reverse transcription PCR using a template RNA extracted from a C57BL/6J liver using a PrimeScript™ II 1st strand cDNA Synthesis Kit (TAKARA Bio, Japan). The coding sequences of the primers used are listed in Table S3 ...
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bioRxiv - Cell Biology 2022Quote: ... 200 μM A/C heterodimerizer (Takara) or ethanol control ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA-seq libraries were prepared using SMARTer Stranded RNA-Seq kit (Clontech). Initially ...
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bioRxiv - Developmental Biology 2022Quote: ... using Xfect (Takara) according to the manufacturer protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and then was transcribed to cDNA using the Clontech SMARTer PCR cDNA Synthesis Kit (Clontech, Mountain View, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Rapalog (A/C Heterodimerizer) was purchased from Takara (Cat #635056) and used at 1µM final concentration ...
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bioRxiv - Cell Biology 2022Quote: ... pEGFP-actin (Clontech), Actin-Chromobody-TagGFP2 (AC-GFP ...
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bioRxiv - Cell Biology 2022Quote: ... The following plasmid constructs were used: pmCherry-N1 (Clontech), pEGFP-N1 (Clontech) ...
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bioRxiv - Cell Biology 2022Quote: ... LifeAct-TagGFP2 (Clontech). Each GFP-conjugated protein was co-expressed with mCherry to highlight neuronal morphology ...
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bioRxiv - Cell Biology 2022Quote: ... pEGFP-N1 (Clontech), pEGFP-actin (Clontech) ...
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bioRxiv - Cell Biology 2022Quote: Y2H was conducted using Matchmaker Gold Yeast Two-Hybrid System (Clontech). The coding sequences (CDS ...
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bioRxiv - Developmental Biology 2022Quote: ... mouse anti-mCherry (1:200, Takara Bio/Clontech Laboratories, 632543), rat anti-BrdU (1:250 ...
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bioRxiv - Cancer Biology 2022Quote: ... Enriched lentiviruses were infected into ATL cell lines using RetroNectin (TaKaRa) with spin infection (3,000rpm ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was PCR-amplified using the PrimeSTAR® GXL DNA Polymerase (Takara Bio, Dalian, China) and the primers annealing at the ends of the gene and carrying the attB sequences (Supplemental table 1 ...
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bioRxiv - Cell Biology 2022Quote: ... washing with cold PBS (Takara), and resuspension in lysis buffer [150 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... GFP (Clontech), and β actin (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... GST tags were cleaved by HRV-3C Protease (Takara), which released the recombinant proteins from the beads ...
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bioRxiv - Neuroscience 2022Quote: ... with the mCherry sequence by the In-Fusion HD Cloning Plus kit (Takara, 638910). For the transposon-based p53 reporter assay performed on KOLF2.1J ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein concentration was found to be 0.9 µg/µl using BCA Protein Assay Kit (TaKaRa).
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bioRxiv - Molecular Biology 2022Quote: ... protein was estimated using BCA (TaKaRa), and 100 µg protein was incubated with 20 µl packed protein G-sepharose beads (Sigma P3296 ...
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bioRxiv - Molecular Biology 2022Quote: ... the cell pellets were resuspended in the prepared cell lysis buffer (0.1% Triton-X, 20 mM Tris, 100 mM NaCl) and ProteoGuard EDTA-Free protease inhibitor cocktail (Takara) for 30 minutes followed by sonication in ice cold water for 40 minutes (75 amplitude ...
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bioRxiv - Developmental Biology 2022Quote: ... using TB Green Premix Ex TaqII (Tli RNaseH Plus) (Takara, RR820). Gene expression was normalized to the housekeeping gene ...