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Citations for Takara Bio :
8051 - 8100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... were used to prepare RNA-Seq libraries using the SMART-Seq v.4 Kit (Clontech) as previously described42 ...
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bioRxiv - Molecular Biology 2022Quote: ... the 5′ and 3′’ untranslated regions (UTRs) of the virus were determined using a SMARTer®RACE 5′/3′ Kit (Takara Bio, USA). Viral RNA isolated in August ...
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bioRxiv - Molecular Biology 2022Quote: ... transformed into Escherichia coli DH5α (TaKaRa, Dalian, China) and finally sequenced at Shanghai Sangon Biotech Co ...
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bioRxiv - Molecular Biology 2022Quote: ... sub-cloned into the pMD 19-T vector (TaKaRa, Dalian, China), transformed into Escherichia coli DH5α (TaKaRa ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μL of TB Green Premix Ex Taq 2 (TliRNaseH Plus; Takara, Dalian, China), 0.8 μL of each primer (Table 1) ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR was performed using PrimeSTAR Max DNA polymerase (Takara, Dalian) to amplify the predicted CYP9A genes under the following cycling conditions ...
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Bipartite viral RNA genome heterodimerization influences genome packaging and virion thermostabilitybioRxiv - Molecular Biology 2022Quote: The primers used to generate the In-Fusion cloning (TaKaRa) fragments were listed in Table S1 ...
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bioRxiv - Molecular Biology 2022Quote: ... the plasmids were cleaved using restriction enzymes and purified using electrophoresis and NucleoSpin Gel & PCR Clean-up kit (Takara bio, Shiga, Japan). The copy number of each standard DNA was calculated based on the concentration quantified with the Qubit 3 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... coli HST08 competent cells (TaKaRa Bio Inc., Japan) for cloning ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products were purified using NucleoSpin Gel and PCR Clean-up (TaKara Bio Inc.), and then analyzed and quantified by Nanodrop spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... pAgos were pulled down using Co2+-Talon Metal Affinity Resin (Takara) as described previously (26) ...
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bioRxiv - Molecular Biology 2022Quote: ... BCA assays using BCA assay kit (TaKaRa) were performed before boiling the lysed cells or medium mixed with 1/3 volume of 4× sample buffer for 10-15 minutes at 90°C ...
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bioRxiv - Molecular Biology 2022Quote: ... coli JM109 competent cells (TaKaRa Bio Inc.) for amplification ...
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bioRxiv - Molecular Biology 2022Quote: ... pAgos were pulled down using Co2+-Talon Metal Affinity Resin (Takara) as described previously (19) ...
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bioRxiv - Molecular Biology 2022Quote: The yeast two hybrid assay was performed as indicated in MATCHMAKER GAL4 two-hybrid system 3 (Clontech). The protein coding regions of genes used in this study were amplified from Guy11 cDNA with primer pairs listed in Table 1.1 ...
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bioRxiv - Molecular Biology 2022Quote: ... coli JM109 cell pellets by using NucleoBond Xtra Midi (TaKaRa Bio Inc.) and protocol for low copy plasmids ...
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bioRxiv - Molecular Biology 2022Quote: ... Takara LA HS Polymerase (Takara RR042) was used with 16426 Forward (5’-CCGCACAAGAGTGCTACTCTCCTCGCTC-3’ ...
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bioRxiv - Molecular Biology 2022Quote: ... AP21967 (1 μM, TaKaRa).
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bioRxiv - Neuroscience 2022Quote: ... and then cloned into pGEMHE using In-Fusion HD Cloning Kit according to the manufacturer’s instructions (Clontech).
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bioRxiv - Neuroscience 2022Quote: ... 40U/mL of RNAse inhibitor (Takara: 2313B)] ...
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bioRxiv - Bioengineering 2022Quote: SMARTer Stranded Total RNAseq Kit v2 - Pico Input Mammalian Components (Takara, Cat No 634419)
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bioRxiv - Bioengineering 2022Quote: SMARTer RNA Unique Dual Index Kit – 96U Set A (Takara, Cat No 634452)
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was subjected to immobilized metal affinity chromatography (IMAC) with talon resin (Takara Bio USA). The bound protein was washed with TBS buffer containing 0.02% GDN and 20 mM imidazole and eluted with TBS buffer containing 0.02% GDN and 250 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... using SARS-CoV-2 S as template and subcloned into pcDNA3.3 vector with a C-terminal hexahistidine tag using In-Fusion cloning technology (Takara Bio USA, Inc.). RBD of SARS-CoV S was cloned into pcDNA3.3 vector from pcDNA3.1-SARS-Spike (Addgene plasmid#145031) ...
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bioRxiv - Biochemistry 2022Quote: ... and the next 1μg of total eligible RNA was reverse transcribed using the cDNA synthesis kit (Code No. 6110A, Takara, Japan) for quantitative real-time PCR (RT-qPCR) ...
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bioRxiv - Bioengineering 2022Quote: ... Total RNA was isolated using NucleoSpin® RNA kit (Takara/Clontech 740955), followed by cDNA synthesis using High Capacity cDNA Reverse Transcription kit (ThermoFisher 4368814 ...
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bioRxiv - Biochemistry 2022Quote: ... Both pFCDUET-YopH phosphatase and pGKJE8-GroEl/GroEs chaperones plasmids (Takara chaperone plasmid set cat. # 3340) were used for co-expression experiments ...
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bioRxiv - Biochemistry 2022Quote: ... S domains and variants were purified similar to S protein using nickel resin (10 mL/L, His60 Ni2+ superflow resin, Takara cat# 635664) with wash buffer (25 mM MES ...
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bioRxiv - Bioengineering 2022Quote: ... 1 µl purified cDNA was amplified with an Advantage HF 2 PCR kit (Takara) in a 25 µl reaction containing 0.5 µl forward primer (10 µM ...
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bioRxiv - Bioengineering 2022Quote: ... concentrated overnight with Lenti-X (Takara), and frozen at −80°C ...
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bioRxiv - Bioengineering 2022Quote: ... were transiently transfected using calcium phosphate (Takara) and retrovirus-containing supernatants were collected 2 days later ...
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bioRxiv - Bioengineering 2022Quote: ... Retrovirus was centrifuged for 2 hrs at 2560rcf at 320C onto wells pre-coated with RetroNectin (Takara). Wells were rinsed with PBS and CD8 T cells were added at 1×106 cells/mL in complete RPMI supplemented with 50 U/mL IL-2 and mouse T-activator Dynabeads (ThermoFisher ...
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bioRxiv - Biophysics 2022Quote: ... 100 µL of the above mixtures were loaded on 100 µL of TALON resin (Takara Bio, USA), in 200 µL thin-walled PCR tubes and incubated for 5 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... ACLY lentivirus constructs were generated by inserting ACLY variants with an N-terminal MYC tag into pLVX-IRES-Puro (Clontech, 632183). All DNA constructs were verified by Sanger sequencing.
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bioRxiv - Cell Biology 2022Quote: ... HeLa-KRAS(G12V) cells were maintained in 10% tetracycline-free FBS (Takara Bio, 631101) and KRAS(G12V ...
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bioRxiv - Cell Biology 2022Quote: Transient transfections for all the cell lines were performed using Xfect transfection reagent from Takara (Cat# 631318) in 6 well plates (Falcon ...
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bioRxiv - Biophysics 2022Quote: ... 10 μL of the reaction was then transformed into 100 μL of StellarTM competent cells (Takara Bio USA) and plated on kanamycin plates ...
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bioRxiv - Biochemistry 2022Quote: ... passed through Ni-NTA spin column (Takara Clontech), washed and column eluted with 500 mM Imidazole ...
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bioRxiv - Cell Biology 2022Quote: ... Cytosolic GFP (pEGFP-N1) and mCherry (pmCherry-N1) vectors were purchased from Clontech. H2B-mCherry was a gift from Robert Benezra (Addgene plasmid #20972 ...
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bioRxiv - Cell Biology 2022Quote: ... LVs were concentrated using LentiX-Concentrator (Takara Bio) and the pellet was resuspended up to 1 mL in serum-free Opti-MEM ...
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bioRxiv - Cell Biology 2022Quote: ... GFP-Eps8-ΔCAP and IRSp53-mCherry were PCR amplified using PrimeSTAR GXL DNA Polymerase (Takara Bio) and the amplicons were extracted from a 1% agarose gel (Monarch Gel Extraction Kit ...
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bioRxiv - Cell Biology 2022Quote: ... and mRNA reverse transcription was performed with the PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Japan). The qPCR was performed using SYBR Green qPCR Master Mix (Takara ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR purified integrin β5 with engineered flanking restriction sites (Xho-I/BamHI) was subcloned into the multi-cloning sites of pEGFP-N1 (Clontech) to encode an in-frame fusion protein with the carboxy-terminal EGFP-tag (pEGFP-N1-Integrin β5 ...
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bioRxiv - Cell Biology 2022Quote: ... The qPCR was performed using SYBR Green qPCR Master Mix (Takara, Japan). The qPCR results were analyzed by 2-ΔΔCt method ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% tetracycline-free fetal bovine serum (FBS, TaKaRa Cat. #631368), 2 mM L-glutamine (Gibco) ...
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bioRxiv - Cancer Biology 2022Quote: ... and converted into cDNA with the PrimeScriptTM RT reagent kit (Takara, Diatech Lab Line, Ancona, Italy). RT-qPCR was performed with 25 ng of the template cDNA using the qPCRBIO SyGreen 2X (PCR Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... The amplicons were then cloned into the pJFRC7 5x UAS vector (ref) using Infusion cloning kit (Takara Bio, catalog #638948). Sequenced clones were sent to BestGene for injection and stock generation.
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bioRxiv - Microbiology 2022Quote: A DNA matrix (Supplementary Table 5) was prepared with CloneAmp HiFi PCR Premix (Takara) using primer pair 56/60 (Supplementary Table 4 ...
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ADAR2 deaminase activity promotes Th17 effector function and protects against intestine inflammationbioRxiv - Immunology 2022Quote: ... The MSCV wildtype murine ADAR2 overexpression construct was generated using the In-Fusion HD Cloning Plus kit (Takara Bio). Specifically ...
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bioRxiv - Cell Biology 2022Quote: ... and the construct was cloned into pLVX-IRES-puro vector (Clontech). SEPT6(ΔAH)-GFP was generated by removing aa 355-372 of SEPT6 from SEPT6-GFP-pLVX-IRES-puro via PCR ...