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Citations for Takara Bio :
8251 - 8300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: iPSCs SFCi55 and hESCs RUNX1-GFP were plated at 3 × 105 cells per a well of a 6 well plate and reverse transfected with 2 μg of DNA using the Xfect Transfection reagent (Clontech) and analyzed 2 days later.
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bioRxiv - Developmental Biology 2022Quote: ... For RACE analysis we used the SMARTer® RACE 5’/3’ Kit (Takara) according to manufacturer’s recommendations (see Supplementary Table 8 for the list of primers used).
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bioRxiv - Developmental Biology 2022Quote: The total RNA was isolated using the Trizol Reagent Kit (9109, Takara, Japan), digested with RQ1 deoxyribonuclease I (Promega ...
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bioRxiv - Developmental Biology 2022Quote: ... the cDNA was amplified using a SYBR Premix Ex Taq Kit (TaKaRa) on the CFX96 Touch™ Real-Time System (Bio-Rad) ...
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bioRxiv - Developmental Biology 2022Quote: ... and reverse-transcribed into cDNA with the Prime Script Reverse Transcriptase Reagent Kit (Takara, Japan). For real-time PCR ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was generated by using SMARTer PCR cDNA Synthesis Kit (Clontech). Gene-specific primers were designed for each gene of interest (Supp ...
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bioRxiv - Developmental Biology 2022Quote: ... in 40 μl of N2B27 medium (Takara Bio). The plate was centrifuged at 750 rpm for 2 minutes to promote deposition and aggregation of the cells and was then incubated at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Genome fragments of each AQP gene were PCR-amplified using MightyAmp DNA polymerase ver.3 (Takara Bio) with the following primers ...
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bioRxiv - Developmental Biology 2022Quote: ... or PrimesSTAR GXL DNA polymerase (Takara Bio) with the following primers ...
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bioRxiv - Developmental Biology 2022Quote: ... polyclonal (Code No. M142, Takara bio); Monoclonal Anti-N-Cadherin/A-CAM (Clone GC-4 ...
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bioRxiv - Cell Biology 2022Quote: ... The pEGFP-P2A-RFP expression vector (pEGFP-N1 Clontech backbone) was a kind gift from Prof ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA was extracted from crushed tissue or cell lysate using the TRIzol reagent protocol (TAKARA/Thermo). cDNA was synthesized using Superscript III (Thermo) ...
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bioRxiv - Developmental Biology 2022Quote: ... RT-PCR kit (Takara, Tokyo, Japan) was used to construct reverse transcription to collect cDNA ...
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bioRxiv - Developmental Biology 2022Quote: ... This amplified fragment was named N-Cad-M and was subcloned into the 5’ side from P2A peptide (ATNFSLLKQAGDVEENPGP) of the pCAG-P2A-H2B-mCherry vector by In-Fusion Cloning (Takara, Japan). To visualize the membrane of cells that express N-Cad-M ...
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bioRxiv - Developmental Biology 2022Quote: Cloning and mutagenesis were performed using In-Fusion restriction-free cloning (Takara). Reporter gene constructs were cloned by inserting PCR amplified promoter elements into the pPD95.75 vector (Fire Vector Kit) ...
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bioRxiv - Developmental Biology 2022Quote: ... Reverse transcription was performed with the PrimeScript High Fidelity RT-PCR Kit (Takara, Cat. R022A). RT-PCR was run on a 7900HT Fast RT-PCR instrument (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... packaging (pCAG-HIVgp) and envelope (pCMV-VSV-G-RSV-Rev) plasmids were co-transfected into Lenti-X 293T cells (Takara Bio) using Fugene 6 reagent (Promega) ...
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bioRxiv - Cell Biology 2022Quote: Total RNA extraction and reverse transcription of RNA into cDNA were performed based on the method described as manufacturer’s protocol of Prime Script TMΠ 1stStrand cDNA Synthesis Kit andTB GreenTM Premix Ex TaqTM II (TaKaRa, Beijing, China). qRT-PCR was performed with ChamQ Universal SYBR qPCR Master Mix (Vazyme ...
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bioRxiv - Cell Biology 2022Quote: The collected embryonic ICM and polar TE (as described above) were used for RNA-seq library preparation using the SMART-Seq HT Kit (Takara Bio USA, Inc.) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 5 ng of total RNA was amplified and converted to cDNA using the SMART-Seq v4 Ultra Low Input RNA kit (Clontech Ref. 634889). Afterwards ...
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bioRxiv - Cell Biology 2022Quote: ... the cultured neurons were transfected using the High-Efficiency Ca2+ Phosphate Transfection Kit (Takara Bio). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... A cassette containing the pre-shRNA sequence was inserted into pBAsi-mU6 (Takara Bio). The target sequences of each shRNA are as follows ...
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bioRxiv - Cell Biology 2022Quote: ... The genomic DNA were prepared with GenTLE (Takara Bio, Japan). The fragments were subcloned into the integrated plasmid pJK148 carrying the leu1+ gene ...
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bioRxiv - Cell Biology 2022Quote: ... The purified fragments were integrated into digested vectors by using In-fusion Snap Assembly Master mix (Takara Bio, Japan, 638947). Our lab stock plasmids ...
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bioRxiv - Cell Biology 2022Quote: ... and the supernatant was incubated with 2 mL TALON Metal Affinity Resin (Takara Bio) for 1 h at 4 °C with rotation ...
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bioRxiv - Developmental Biology 2022Quote: ... mouse anti-mCherry (1:200, Takara Bio/Clontech Laboratories, 632543), rat anti-BrdU (1:250 ...
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bioRxiv - Developmental Biology 2022Quote: ... Full-length cDNA was amplified using Ex Taq (Takara Bio) or PrimesSTAR GXL DNA polymerase (Takara Bio ...
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bioRxiv - Developmental Biology 2022Quote: After amplification of the cDNA library using the Smart-Seq v4 Ultra Low Input Kit (Clontech), the library was prepared using the NEBNext Ultra RNA library prep kit for Illumina (New England Biolabs) ...
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bioRxiv - Developmental Biology 2022Quote: ... and reverse-transcribed using PrimeScript II reverse transcriptase (Takara Bio). Full-length cDNA was amplified using Ex Taq (Takara Bio ...
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bioRxiv - Developmental Biology 2022Quote: ... 2017) into the 3’ end of AQP1 in pBS-AQP1 (described above) using the In-Fusion Cloning system (Takara Bio) with the following primers ...
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bioRxiv - Developmental Biology 2022Quote: ... A synthetic CRE sequence flanked by AttB1-AttB2 sites was cloned (by Eurofins) upstream of a IRES2-DsRed-express2 cassette (plasmid pIRES2-DsRed-express2 from Clontech/SakaRa, #632540), producing a plasmid called p4 containing AttB1-CRE-AttB2_IRES2-DsRed-express2 ...
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bioRxiv - Cell Biology 2022Quote: hCEC were transduced with pHAGE-DD-KNL1Mut-dCas9 and a sgRNA vectors and DD-KNL1Mut-dCas9 was stabilized with 100 nM Shield-1 (Takara, #632189) for 9 days ...
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bioRxiv - Cell Biology 2022Quote: Genomic DNA was extracted from HEK 293T cells using a NucleoSpin Blood kit (Takara Bio; 740951) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... the fragments were amplified with PrimeSTAR MAX (Takara Bio, Japan, R045) with the appropriate primer pairs having overlapping sequence ...
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bioRxiv - Cell Biology 2022Quote: ... partial Teneurin2 from KAZUSA cDNA (NCBI AB032953) and RIKEN cDNA (NCBI AK031198) were amplified by PCR and inserted into pEGFP-C1 (Takara Bio). The missing part was complemented by a custom gene synthesis (Eurofins Genomics K.K.) ...
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bioRxiv - Cell Biology 2022Quote: ... into the pLVX vector (Clontech) digested with Not1 and BamH1 restriction enzymes (New England Biolabs) ...
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bioRxiv - Cell Biology 2022Quote: ... and the samples were homogenized using an RNase-free tube and plunger (Takara, 9791A) and then vortexed for 5 seconds ...
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bioRxiv - Cell Biology 2022Quote: ... was added per 50 mg feces and then homogenized using an RNase-free tube and plunger (Takara, 9791A). 100 µL chloroform (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: CDNA synthesis was done using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech) and the library was prepared using Nextera XT DNA Library Prep kit (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... and either used on the same day or concentrated using Lenti-X concentrator solution (Takara Bio, Cat #631231) and snap frozen at −80C.
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bioRxiv - Cell Biology 2022Quote: ... Assembled plasmids were transformed into Stellar chemically competent E.Coli (Takara-Clontech) and plated on appropriate Luria Bertani agar plates ...
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bioRxiv - Cell Biology 2022Quote: ... purchased from Takara Bio (Cat #632189 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cDNA samples were cloned into a pUC19 vector (PCR-amplified with primers 11 and 12) using In-Fusion HD Cloning Kit (Takara). After transformation into Escherichia coli ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The fluorescence intensities of bands corresponding to parasitic RNAs were quantified using ImageJ (NIH) after staining with SYBR Green II (Takara). The concentrations of parasitic RNAs were determined from the intensities based on a dilution series of a standard parasitic RNA (s222 [35]).
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bioRxiv - Evolutionary Biology 2022Quote: ... and in vitro transcription with T7 RNA polymerase (Takara). All transcribed RNAs were purified using the RNeasy Mini Kit (QIAGEN).
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bioRxiv - Evolutionary Biology 2022Quote: ... The measurement was performed after diluting the droplets 100-fold with 1 mM EDTA (pH 8.0) and using One Step TB Green PrimeScript PLUS RT-PCR Kit (Takara).
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bioRxiv - Evolutionary Biology 2022Quote: ... The cDNA of the RepNDK-RNA clone and the plasmids for all other RNA clones were subjected to digestion with Sma I (Takara) and in vitro transcription with T7 RNA polymerase (Takara) ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were clarified by centrifugation at 3,000 x g and incubated with Talon® immobilized metal affinity resin (Takara #635502) for 2 hours at 4ºC ...
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bioRxiv - Cell Biology 2022Quote: ... GFP (JL-8, Clontech, #632381). Secondary antibodies used included ...
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bioRxiv - Cell Biology 2022Quote: ... The sequencing library was prepared using the SMART-Seq v4 Ultra Low Input RNA kit (Takara, Kusatsu, Japan), and mRNA-seq on an Illumina NovaSeq 6000 (paired-end ...