Labshake search
Citations for Takara Bio :
7901 - 7950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... 10 ng of linearized pDEST32 empty vector was co-transformed with 3 μl of PCR product to achieve recombinational cloning by gap-repair in Y2H Gold yeast strain (Clontech) expressing AD-fused CP204L (pPC86 vector) ...
-
bioRxiv - Microbiology 2022Quote: ... pGFP-N1 plasmid (Clontech, GenBank accession # U55762) was used for GFP expression ...
-
bioRxiv - Microbiology 2022Quote: ... coli Stellar cells (Takara) for non-R6K origin of replication-based vectors or PIR2 cells (Invitrogen ...
-
bioRxiv - Microbiology 2022Quote: ... coli Stellar (Takara) and E ...
-
bioRxiv - Microbiology 2022Quote: ... using the In-Fusion HD cloning system (Takara). In-Fusion reactions were transformed into E ...
-
bioRxiv - Microbiology 2022Quote: ... and amplification was performed using the SYBR Premix Ex Taq II (Takara, Japan) solution ...
-
bioRxiv - Microbiology 2022Quote: ... we amplified the gene (ORF2) encoding the VP1 sequence of the HuNoV Sydney strain by PrimeStar HS DNA polymerase (Takara Bio, cat. #r010a) and replaced it with the sequence encoding GFP in the pT7-RRV-NSP3-GFP constructed previously (12 ...
-
bioRxiv - Microbiology 2022Quote: ... The remaining RNA was converted into cDNAs using the cDNA EcoDry Premix (Clontech, USA). Quantitative real-time PCR was performed using 10-fold diluted cDNAs as the template ...
-
bioRxiv - Microbiology 2022Quote: ... The insertion was conducted by recombination of overlapping sequences using Infusion cloning (Clontech, USA), as previously reported (Park et al. ...
-
bioRxiv - Microbiology 2022Quote: ... and the supernatant was loaded into 0.5 ml of a Talon metal affinity resin (Clontech) equilibrated with binding buffer ...
-
bioRxiv - Microbiology 2022Quote: ... cDNA was synthesized using the PrimeScript RT Master Mix (Takara Bio, Shiga, Japan), and real-time PCR was performed using the Luna Universal qPCR Master Mix (New England Biolabs ...
-
bioRxiv - Microbiology 2022Quote: ... we determined the correlation of measurement with a commercial qPCR kit (#RC202A, TaKaRa, Shiga, Japan). The commercial qPCR kit targeted he BLV pol gene and RPPH1 for housekeeping ...
-
bioRxiv - Molecular Biology 2022Quote: ... and cDNAs were synthesized using the PrimeScript RT reagent kit with gDNA eraser (TAKARA). A random hexamer was used for cDNA synthesis ...
-
bioRxiv - Microbiology 2022Quote: ... and purified by Lenti-X Concentrator (TaKaRa). To measure the infectivity ...
-
bioRxiv - Microbiology 2022Quote: ... S-6P-NanoLuc proteins were released after HRV 3C protease (TaKaRa) treatment overnight at 4°C.
-
bioRxiv - Neuroscience 2022Quote: ... where the lysing solution is composed of single-cell lysis buffer (Takara #635013): 1x RNase Inhibitor Murine (NEB) ...
-
Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneuronsbioRxiv - Neuroscience 2022Quote: ... Total RNA was subjected to reverse transcription according to the protocol of Prime Script 1st Strand cDNA Synthesis kit (Takara Bio USA). Real time PCR analysis was performed using a Step One Plus real-time PCR system (Applied Biosystems ...
-
bioRxiv - Neuroscience 2022Quote: ... Viral particles were concentrated using the Lenti-X Concentrator (Clontech, 631232) and the viral pellets were resuspended in PBS for astrocyte infection.
-
Activity-regulated growth of motoneurons at the neuromuscular junction is mediated by NADPH oxidasesbioRxiv - Neuroscience 2022Quote: ... Rabbit-anti-dsRed (1:1000) (ClonTech Cat. No. 632496), Mouse nc82 (Bruchpilot ...
-
bioRxiv - Neuroscience 2022Quote: ... was digested using HinDIII and XhoI to remove the PSMD2 CDS and the amplified Zic3 gene was cloned using Infusion kit (Clontech, USA) as per manufacturer’s instruction. ...
-
bioRxiv - Neuroscience 2022Quote: ... Reverse transcription with 1 μg of total RNA was conducted using PrimeScript™ RT Master Mix (Takara) following the manual ...
-
bioRxiv - Neuroscience 2022Quote: AAV vectors (serotype DJ) were generated using the pHelper (AAVpro Helper-free system, Takara), pAAV-DJ (Cell Biolabs) ...
-
bioRxiv - Neuroscience 2022Quote: ... triple transfection of HEK-293T cells was performed using the pHelper plasmid (TaKaRa, USA), trans-plasmid with PHP.B capsid (Ben Deverman ...
-
bioRxiv - Neuroscience 2022Quote: ... and anti-DsRed (1:500, rabbit, Takara Bio ...
-
bioRxiv - Neuroscience 2022Quote: ... Viral titers were measured using AAVpro Titration Kit (#6233, Takara) or THUNDERBIRD SYBR qPCR Mix (QPS-201 ...
-
bioRxiv - Neuroscience 2022Quote: ... single GFP+ cells were captured into 96-well plate with 11.5 μl lysis buffer and RNase Inhibitor without the CDS IIA Oligo (Takara Bio, Mountain View, CA), and stored at −80°C until used for construction of cDNA libraries ...
-
bioRxiv - Neuroscience 2022Quote: ... rabbit anti-dsRed (TaKaRa Bio, cat. no. 632496, 1:250), chicken anti-GFP (Aves ...
-
bioRxiv - Neuroscience 2022Quote: Single-cell RNA-seq libraries were constructed according to the SMART-Seq Single PLUS protocol (Takara Bio, Mountain View, CA). Frozen plates were thawed on ice ...
-
bioRxiv - Neuroscience 2022Quote: ... We constructed the dlg1[4K] donor plasmid (Fig. 1) by joining five fragments into a minimal (AMP and ORI) plasmid backbone by In Fusion assembly (Takara, no. 639649). A list of primers used ...
-
bioRxiv - Neuroscience 2022Quote: ... rabbit anti-dsRed (1:200, Takara, #632496), Alexa488 goat anti-mouse (1:500 ...
-
bioRxiv - Neuroscience 2022Quote: ... This sequence was cloned into the EcoRI-NotI sites of the CRISPRa plasmid using In-Fusion cloning (Takara). All guide sequences and their genomic locations are listed in Supplementary Table S1 and PCR primers used to verify editing are listed in Supplementary Table S2.
-
bioRxiv - Neuroscience 2022Quote: ... AAV vectors were purified using the AAVpro Purification Kit All Serotypes (#6666, Takara). Viral titers were measured using AAVpro Titration Kit (#6233 ...
-
bioRxiv - Neuroscience 2022Quote: ... and the AAVpro 293 T cell line (#632273, Takara) following the manufacturers’ instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... 2016) with the design enabling two guide cassettes to be inserted into one SaCas9 plasmid by In-Fusion cloning (Takara). Guide sequences were designed using the online CRISPOR design tool (Haeussler et al ...
-
bioRxiv - Neuroscience 2022Quote: ... This sequence was cloned into the EcoRI-NotI sites of the modified plasmid 61591 using In-Fusion cloning (Takara). The ‘empty vector’ control contained CMV-promoter driven dSaCas9-KRAB but no guide sequences ...
-
bioRxiv - Developmental Biology 2022Quote: ... on a Thermal Cycler Dice Real Time System III (TaKaRa) with Thunderbird SYBR qPCR Mix (TOYOBO ...
-
bioRxiv - Developmental Biology 2022Quote: Preparation of total RNA-seq libraries was performed using SMART-Seq Stranded Kit (Clontech), according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2022Quote: ... The GFP-Fluc cassette was then inserted into the TgDHFRpyr entry vector using In-Fusion HD cloning (Takara Bio.) that resulted in the triple gene entry plasmid (GFP_Fluc_TgDHFRpyr) ...
-
bioRxiv - Developmental Biology 2022Quote: ... Individual ASO-targeted sequences were mutated by inverse PCR with PrimeSTAR GXL DNA polymerase (TaKaRa) and specific primer sets (Supplementary Dataset 5) ...
-
bioRxiv - Developmental Biology 2022Quote: ... 30 ZP-free embryos were lysed in 1x Lysis Buffer containing RNase inhibitor (0.2 IU/µl, from SMART-Seq Stranded Kit, Clontech), directly ...
-
bioRxiv - Developmental Biology 2022Quote: ... using the In-Fusion cloning kit (#639650, Takara Bio USA, Inc.). Primers and gRNA oligos are listed in Table S5 ...
-
bioRxiv - Developmental Biology 2022Quote: ... The final BAC was purified with Nucleobond BAC 100 kit (Clontech) and co-injected with I-Sce I into one-cell-stage zebrafish embryos ...
-
bioRxiv - Developmental Biology 2022Quote: ... The PCR procedure followed the instructions for Tks Gflex DNA polymerase (TaKaRa Bio Inc., Shiga, Japan). The amplified PCR fragments were subcloned into the pGEM T Vector system (Promega Corporation ...
-
bioRxiv - Developmental Biology 2022Quote: ... rabbit anti-DsRed (Takara, 632496), and mouse anti-Tnnt (ThermoFisher ...
-
bioRxiv - Developmental Biology 2022Quote: ... anti-DsRed (rabbit, 1/300, Takara, 632496), anti-Parvalbumin (mouse IgG1 ...
-
bioRxiv - Developmental Biology 2022Quote: ... first-strand cDNA synthesis and subsequent cDNA amplification by 22 cycles of polymerase chain reaction (PCR) were performed using the SMARTer stranded RNA-seq kit (Takara). The synthesized first-strand cDNA and amplified cDNA were purified using Ampure XP Beads (Beckman Coulter) ...
-
bioRxiv - Developmental Biology 2022Quote: ... and Thermal cycler Dice Realtime TP800 (Takara) and sequenced in single-end runs (150 cycles ...
-
bioRxiv - Developmental Biology 2022Quote: ... The libraries were quantified using the Library quantification kit (Takara) and Thermal cycler Dice Realtime TP800 (Takara ...
-
bioRxiv - Developmental Biology 2022Quote: qPCR was done with SYBR Premix Ex Taq from Takara (Catalog: RR420) and StepOnePlus Real-Time PCR System for Act5C and α-tub84B ...
-
bioRxiv - Genetics 2022Quote: ... 2 μL of DNA loading buffer (9157, TAKARA, Kusatsu, Japan) was added to the reaction and incubated at 65 ℃ for 5 min ...