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Citations for Takara Bio :
8151 - 8200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... For reverse transcription 2 µg RNA were translated into cDNA using the “RNA to cDNA EcoDry” Kit (Takara Bio USA, Kusatsu, Japan) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... at SacI and MulI using InFusion cloning (TaKaRa), and screened using PCR and Sanger sequencing ...
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bioRxiv - Cancer Biology 2022Quote: ... to the 3’ end of the GFP at the PmeI site using InFusion Cloning (TaKaRa) and screened with PCR and restriction digests (Supplementary Table S8) ...
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bioRxiv - Cancer Biology 2022Quote: ... and the annealed barcode was inserted by InFusion cloning (TaKaRa) 3’ of the GFP and 5’ of the polyA sequence ...
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bioRxiv - Biochemistry 2022Quote: ... the cleavage mixture was applied to a gravity flow column packed with 8 mL TALON cobalt resin (Takara) equilibrated in AEX buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified lysates were incubated with TALON® metal affinity resin (Takara Bio USA) for 1 h with end-over-end rotation at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... or Talon® (Clontech) resins accordingly ...
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bioRxiv - Biochemistry 2022Quote: ... Stellar competent cells (Takara 636763) were transformed with the Gibson assembly products and spread on LB plates containing ampicillin ...
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bioRxiv - Biochemistry 2022Quote: ... Five hundred nanogram of total RNA was reverse transcribed by PrimeScript RT reagent kit with gDNA eraser (Takara), and qRT-PCR was performed using KAPA SYBR FAST Master Mix (2X ...
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bioRxiv - Biochemistry 2022Quote: ... by In-fusion cloning kit (Takara). pIZ-Siwi-D670A and pIZ-BmAgo3-D697A mutants were generated by site-directed mutagenesis.
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bioRxiv - Biochemistry 2022Quote: ... and the Thermal Cycler Dice Real Time System (Takara). The primer sequences for real-time PCR are listed in Table EV1.
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bioRxiv - Biochemistry 2022Quote: ... The oligonucleotides at HPLC purity were obtained from TaKaRa company (Dalian ...
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bioRxiv - Bioengineering 2022Quote: ... concentrated with Lenti-X concentrator (Clontech, Mountain View, CA), and stored at −80C until use.
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bioRxiv - Biochemistry 2022Quote: ... testis and supraneural bodies by using RNAiso reagent (TaKaRa, China). Leucocytes were collected from circulated blood ...
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bioRxiv - Bioengineering 2022Quote: ... the 96-well plate can be coated with RetroNectin® (Clontech/Takara ...
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bioRxiv - Biochemistry 2022Quote: ... and subcloned into the appropriated vector either using the InFusion cloning system following the manufacturer’s protocol (Takara). Mutations into the TMD were introduced by site-directed mutagenesis using the Quick Change II kit following the manufacturer’s instructions (Agilent Technologies) ...
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bioRxiv - Bioengineering 2022Quote: ... Total RNA was isolated using NucleoSpin® RNA kit (Takara/Clontech 740955), followed by cDNA synthesis using High Capacity cDNA Reverse Transcription kit (ThermoFisher 4368814 ...
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bioRxiv - Biochemistry 2022Quote: ... or NHE7 (Milosavljevic et al., 2014) (pIRES hygro vector, Clontech) were used to express these NHEs at the plasma membrane by repeated acute acidifications in the presence of 120 mM extracellular NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... PS120 cells stably transfected with NHE6 (pIRES neo vector, Clontech) or NHE7 (Milosavljevic et al. ...
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bioRxiv - Bioengineering 2022Quote: ... RNA (2 µg) was reverse transcribed into cDNA with the RT Reagent Kit with gDNA Eraser (Takara, Japan, RR047A). qPCR was performed using a SYBR Premix Ex Taq kit (Takara ...
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bioRxiv - Bioengineering 2022Quote: ... qPCR was performed using a SYBR Premix Ex Taq kit (Takara, Japan, RR420A) on a QuantStudio 6 Flex System (Thermo ...
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bioRxiv - Bioengineering 2022Quote: ... gene segments of HA7 and NA9 ectodomains were cloned downstream of the cytomegalovirus promoter in pTriEx3 expression vector (Clontech). The vectors were transfected into A549 cells in 96-well microtiter plate using TransLT transfection reagent (Mirus ...
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bioRxiv - Biochemistry 2022Quote: ... mCherry-H2B HeLa cell lines were cultured in DMEM (PAM Biotech) supplemented with 10% FBS (Clontech), 2 mM L-glutamine (PAN Biotech) ...
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bioRxiv - Bioengineering 2022Quote: ... All the fragments were ligated sequentially and inserted into XhoI site on pABpaR2pX by In-Fusion HD Cloning Kit (Clontech Laboratories, Inc.) To generate the transfer vector for NA9-Bac ...
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bioRxiv - Bioengineering 2022Quote: The transfer vector pABpaR2pX was constructed by inserting a DsRed2 reporter gene driven by pag promoter(46) (p-pag) into the EcoRV restriction enzyme site of pBacPAK8 (Clontech Laboratories, Inc.). cDNAs encoding HA7 and NA9 were synthesized by GenScript ...
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bioRxiv - Bioengineering 2022Quote: ... prepared with a SMARTer Stranded Total RNA-Seq Kit v3 – Pico Input Mammalian kit (Takara Bio Inc., Japan) with 0.75 ng and bulk-sequenced using 100 bp paired-end reads at a depth of 50 million reads ...
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bioRxiv - Bioengineering 2022Quote: ... inserts were directly ligated into the pLVX-TRE3G vector (Clontech, CA). To remove the MTS and produce constructs of untargeted STHs ...
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bioRxiv - Biochemistry 2022Quote: A DNA fragment coding C-terminally His-tagged Gtsf1 or Gtsf1L was amplified by PCR and cloned into pCold vector (Takara) by In-fusion cloning kit (Takara) ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... pRSFDUET-1-EIF6 plasmid was co-transformed with pG-KJE8 expressing five bacterial chaperones (5-Ch: DnaK-DnaJ-GrpE, GroES, GroEL) or pG-TF2 expressing 3 bacterial chaperones (3-Ch: GroES, GroEL, TF) (Takara Biosciences) in BL21 (DE3 ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... pRSF-DUET-1-EIF6 construct was used to express eIF6 and the pTf16 (Takara Biosciences) construct was used to express the TF chaperone ...
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bioRxiv - Biochemistry 2022Quote: ... or anti-GFP antibody (Clontech) for 1 hour at room temperature followed by a second incubation with HRP-conjugated secondary antibody (Santa Cruz Biotechnology) ...
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bioRxiv - Cell Biology 2022Quote: The AAV-sgApc virus was produced from the pAAV-Guide-it-Down construct (Clontech Laboratories Inc., 041315) using assembly primers:
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bioRxiv - Cell Biology 2022Quote: ... and preserved in phosphate-buffered saline (PBS; TaKaRa) at 4 °C until use ...
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bioRxiv - Biochemistry 2022Quote: shTnsB mutants (Fig. 7) were generated in the pHelper vector using the In-Fusion cloning kit from Takara and primers containing the desired point mutations ...
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bioRxiv - Biochemistry 2022Quote: ... using the InFusion HD cloning kit (Clontech). The expression plasmid for C-terminal 6×His-tagged Sup35NM-mCherry was constructed by inserting the sequence encoding mCherry into the expression plasmid for Sup35NM based on pET29b (residues 1–253 ...
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bioRxiv - Biochemistry 2022Quote: ... MBL) and HRP-labeled IgG detector (1/5,000 dilution from the product, Western BLoT Rapid Detect v2.0, Takara Bio) were used as primary and secondary antibodies ...
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bioRxiv - Biochemistry 2022Quote: ... 127926 and 127924). shTnsB mutants (Fig. 7) were generated in the pHelper vector using the In-Fusion cloning kit from Takara and primers containing the desired point mutations ...
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bioRxiv - Biochemistry 2022Quote: The expression plasmid for N-terminal 6×His-tagged mCherry-Nter was constructed by inserting the sequence encoding DmAgo2 Nter (residues 1–398) amplified from the native DmAgo2 sequence into pCold I (TAKARA Bio) using the InFusion HD cloning kit (Clontech) ...
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bioRxiv - Biochemistry 2022Quote: The recombinant protein in the soluble fraction was then purified using TALON Metal Affinity Resin (Clontech Laboratories, CA, USA) per manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: A DNA fragment coding HA-tagged Dcp2 was amplified by PCR and cloned into pIExZ vector (Izumi et al, 2020) by In-fusion cloning kit (Takara).
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bioRxiv - Biochemistry 2022Quote: cDNA fragment of Gtsf1 was amplified by RT-PCR from BmN4 total RNAs and cloned into pIExZ vector (Izumi et al, 2020) by In-fusion cloning kit (Takara).
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysate was added to TALON CellThru Resin (Takara) and incubated at 4°C for 1h ...
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bioRxiv - Biochemistry 2022Quote: ... by In-fusion cloning kit (Takara). For Gtsf1L ...
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bioRxiv - Biochemistry 2022Quote: A cDNA fragment of Gtsf1L was amplified by RT-PCR from BmN4 total RNAs and cloned into pIZ vector by In-fusion cloning kit (Takara).
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bioRxiv - Biochemistry 2022Quote: ... the same genes were cloned into pEGFP-C3 (Clontech). In all the cases ...
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bioRxiv - Bioengineering 2022Quote: ... PDGF-aa (20 ng/ml; R&D) is also supplemented to RHB-A (Takara). This medium composition will be referred to as RHB-A complete ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with 10% FBS (Clontech), 100 units/mL penicillin ...
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bioRxiv - Cancer Biology 2022Quote: ... the recombinant pAAV-CRE plasmid was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara, Catalog #632608). Cells were harvested three days after transfection and AAV2 was isolated using AAVpro Extraction Solution (Takara ...
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bioRxiv - Biophysics 2022Quote: ... and human CaMKIIK42RD135N with a C-terminal AviTag were cloned into pEGFP-N1 (Clontech) vector ...
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bioRxiv - Biophysics 2022Quote: Site-directed mutagenesis to construct the variants was performed using the PrimeSTAR mutagenesis basal kit (Takara Bio Inc., Kusatsu, Shiga, Japan) with the pPICZαA vector containing the mature Forham1 gene as the template ...