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Citations for Takara Bio :
7751 - 7800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was extracted from whole animals using RNAiso Plus reagent (Takara) and sequenced using NovaSeq 6000 System by Macrogen Corp ...
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bioRxiv - Physiology 2022Quote: ... cDNA synthesis was performed using the Takara SMART-seq v4 PLUS kit (Takara, #634889) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: We amplified the coding sequences of MpSETA and MpICE2 from cDNA derived from mRNA of Tak-1 thalli by PCR using PrimeSTAR Max DNA polymerase or PrimeSTAR GXL polymerase (Takara Bio). Also ...
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bioRxiv - Plant Biology 2022Quote: ... at the SalI and EcoRV restriction sites using an In-Fusion HD Cloning Kit (Takara Bio), which we transferred into the destination vector pGWB50157 using Gateway LR Clonase II Enzyme mix (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2022Quote: ... we amplified the genomic fragment of the 4,194 bp upstream region of the translational initiation site from Tak-1 gDNA using PrimeSTAR Max DNA polymerase (Takara Bio), subcloned it into pENTR1A at the EcoRI restriction sites using the In-Fusion HD Cloning Kit and then transferred it into the destination vector pMpGWB10465 using Gateway LR Clonase II Enzyme mix (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2022Quote: ... Bait and prey constructs were co-transformed into the yeast strain Y2HGold (Clontech) using the Frozen-EZ Yeast Transformation II Kit (Zymo Research ...
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bioRxiv - Synthetic Biology 2022Quote: ... Genomic regions of interest were amplified using Primestar Max DNA polymerase (Takara, # R045A) and primers HGTpcrF ...
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bioRxiv - Immunology 2022Quote: ... Purified DNA was PCR amplified using PrimeSTAR Max (Takara-Clontech, Shiga, Japan) for the variable V4 region (using 515F-806R barcoded primers ...
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bioRxiv - Immunology 2022Quote: ... Purified DNA was PCR amplified using PrimeSTAR Max (Takara-Clontech, Shiga, Japan) for the variable V4 region (using 515F-806R barcoded primers ...
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bioRxiv - Cell Biology 2022Quote: ... plasmids were constructed using In-Fusion HD EcoDry Cloning Kits (Takara) and were verified by Sanger sequencing prior to transfection into mammalian cells.
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bioRxiv - Biochemistry 2022Quote: ... mouse DPPA3 cDNA was sub-cloned into a pGEX4T-3 plasmid using In-Fusion (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: One μg of RNA per kidney half was reverse-transcribed using PrimeScript RT Reagent kit (RR037 TAKARA, Shiga, Japan). Two μl of cDNA was used for quantitative real-time PCR to assess gene mRNA expression ...
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bioRxiv - Cancer Biology 2022Quote: Plasmids encoding human Ankrd26-GFP were cloned by PCR using EST clone 6830677 (NM_014915.2) as template and subcloned into pEGFP-N3 (Clontech) using KpnI und BamHI as restriction sites ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA (2 μg) was used for reverse transcription with the PrimeScriptTM RT reagent Kit (TaKaRa). Quantitative expression assays were performed by using the 2x M5 HiPer SYBR Premix EsTag kit (Mei5bio ...
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bioRxiv - Molecular Biology 2022Quote: ... with primers containing 15 bp tails homologous to cloning sites within the pGL4.10[luc2] vector which is necessary for cloning using the In-Fusion® HD cloning kit (Takara). Primer sequences are indicated in Table S1 ...
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bioRxiv - Genetics 2022Quote: cDNA was generated from 1 μg of total RNA by using the PrimeScript RT Reagent Kit (Takara, Japan) in accordance with the manufacturer’s protocol as previously described [21] ...
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bioRxiv - Genomics 2022Quote: ... PrimeStar GXL DNA polymerase (Takara Bio) was used to amplfy the B2M Cas9-RNP target site using primers 5’-GCTCTTCCGATCTaagctgacagcattcgggc and 5’-GCTCTTCCGATCTgaagtcacggagcgagagag ...
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bioRxiv - Genomics 2022Quote: ... IgG and IgM 5’RACE AIRR-seq libraries were generated using the SMARTer Human BCR Profiling Kit (Takara Bio), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... prior to another round of PCR amplification for 16-18 cycles using either LA-Taq or PrimeSTAR GXL (Takara) at an annealing temperature of 60℃.
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bioRxiv - Genomics 2022Quote: ... PCR amplification was performed for 8-9 cycles using high-fidelity polymerase (LA-Taq or PrimeSTAR GXL, Takara) at an annealing temperature of 60℃ ...
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bioRxiv - Genomics 2022Quote: ... PCR Master Mixkit was purchased from TaKaRa Company ...
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bioRxiv - Developmental Biology 2022Quote: ... pTriplEx2 (Takara) or pZL1 (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... and SMARTScribe reverse transcriptase (Takara Bio, Kusatsu, Japan) (Koning et al. ...
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bioRxiv - Immunology 2022Quote: ... the SMARTer RACE 5’/3’ Kit (Takara) was used following manufacturer’s protocol and using the following primer ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA extraction and reverse transcription were performed using NucleoSpin (Takara, #U955C) or Picopure (Thermo ...
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bioRxiv - Cell Biology 2022Quote: ... VSV-G pseudotyped retroviral particles encoding constructs of choice were prepared using standard protocols using GP2-293 packaging cells (Clontech). Viral supernatants were collected and used to infect target cells by centrifugal inoculation (spinoculation) ...
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bioRxiv - Cell Biology 2022Quote: ... FKBP-PKR was activated with 100 nM of the homodimerization ligand AP20187 (Takara), as previously described(Zappa et al ...
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bioRxiv - Cell Biology 2022Quote: qRT-PCR were performed using the SYBR Premix Ex Taq polymerase (Takara Bio Inc) with the ViiA™7 Instrument (Applied Biosystems) ...
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bioRxiv - Immunology 2022Quote: Genetically modified NK cells were generated as follows: Supernatants containing retroviral particles encoding CAR constructs were spun at 2000g in retronectin (T100B, Takara Bio) coated non-tissue culture 24-well plates for 90 minutes ...
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bioRxiv - Immunology 2022Quote: ... Reverse transcription to cDNA was performed with PrimeScrip RT Master Mix (RR036A, Takara Bio, Japan). The cDNA was used as a template for PCR amplification of the following gene coding sequences (primers shown in Supp ...
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bioRxiv - Immunology 2022Quote: ... Seq Kit (Takara), as per the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2022Quote: Immunohistochemistry of spinal cord cryostat sections (30µm) was performed using the following primary antibodies: Rabbit anti-DsRed (1:3000, Takara Bio, 632496), guinea pig anti-VGLUT1 (1:8000 ...
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bioRxiv - Biochemistry 2022Quote: ... N-terminal Flag-tagged P38α containing 3C protease site was amplified by PCR using CloneAmp HiFi PCR Premix (Takara) and ligated in to pcDNA3 using the aforementioned restrictions sites ...
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bioRxiv - Biochemistry 2022Quote: ... and the DNA was extracted and then DNA libraries were prepared with Smarter Thruplex DNAseq Kit (Clontech, R400674) following the manufacturer’s protocols with amplification for 10-13 circles ...
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bioRxiv - Biochemistry 2022Quote: ALP activities were measured using a Great EscAPe™ SEAP kit (Takara Bio Inc., Shiga, Japan) in lysates of PIGT+ and PIGT− cells ...
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bioRxiv - Biochemistry 2022Quote: ... probes labeled with [α-32P] dCTP by random priming (Takara, cat# 6045) were hybridized to the membrane in PerfectHyb Plus Hybridization buffer (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... An oligonucleotide probe against snoRNA58 labeled with [γ-32P] ATP by T4 polynucleotide kinase (Takara, cat# 2021A) was used as a loading control ...
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bioRxiv - Cancer Biology 2022Quote: ... GCN2 coding sequence was subcloned into the lentiviral vector pLVX-IRES-Hygromycin (Clontech, Cat #632185) using an In-Fusion HD Cloning plus kit (TaKaRa ...
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bioRxiv - Cancer Biology 2022Quote: ... using an In-Fusion HD Cloning plus kit (TaKaRa, Cat. #638920) according to manufacturer’s protocol yielding plasmids pLVX-GCN2-hygro ...
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bioRxiv - Cancer Biology 2022Quote: ... Plasmid pMSCV-GADD34-puro contains the human PPP1R15A (GADD34, NM_014330.5) gene subcloned into the NcoI/EcoRI sites of the retroviral vector pMSCV-puro (Clontech Laboratories, Mountain View, CA).
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bioRxiv - Cancer Biology 2022Quote: ... concentrated using Lenti-X™ Concentrator (TaKaRa, Cat. #631232), and stored in aliquots at −80 °C ...
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bioRxiv - Biochemistry 2022Quote: ... previously subcloned into pIRES2-EGFP vector (Clontech) using XhoI and EcoRI restriction enzyme sites (Yang et ...
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bioRxiv - Biochemistry 2022Quote: ... Zebrafish PAC coding sequence (NP_001278691) was subcloned into pIRES2-EGFP vector (Clontech) using NheI and EcoRI restriction enzyme sites ...
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bioRxiv - Biochemistry 2022Quote: ... Resulting vector and gene fragment were combined via Gibson Assembly (Takara In-fusion Kit) generating pLew100-FLPOLIBRc-PTPPuro ...
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bioRxiv - Cell Biology 2022Quote: ... Conventional (semi-quantitative) PCR was performed using Ex Taq DNA polymerase (TaKaRa). qPCR was performed using Thunderbird SYBR qPCR Mix (Toyobo) ...
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bioRxiv - Molecular Biology 2022Quote: Single cells were prepared using the STORM-seq protocol (referencing the SMART-Seq Stranded Kit – Takara Bio) on the SPT Labtech Mosquito (HV) ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR1 (10 cycles of amplification) was performed with the addition of SMARTer RNA Unique Dual Index Set A and Set B (Takara SMARTer RNA Unique Dual Index Kit-96A). Samples were pooled into quadrants and depletion of ribosomal cDNA was performed ...
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bioRxiv - Neuroscience 2022Quote: The Yeast-Two-Hybrid screening for Jacob interaction partners was performed using a pretransformed human brain cDNA Library in pACT2 (Matchmaker-GAL4 Two-Hybrid II; Clontech) as described previously (Helmuth et al. ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli via InFusion cloning (Takara Bio), according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cas9GCN4 and the catalytic core of chromatinmodifying enzymes were cloned into PiggyBac recipient plasmids by homology arm recombination using In-fusion HD cloning (Takara #639650). Specifically ...