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Citations for Takara Bio :
7701 - 7750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... Reverse transcription was performed using the oligo(dT) primer and avian myeloblastosis virus reverse transcriptase contained in the TaKaRa RNA PCR kit (TaKaRa). RT-qPCR was performed using the Kapa SYBR FAST qPCR kit (Kapa Biosystems ...
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bioRxiv - Genetics 2022Quote: ... galactose/raffinose (Takara Bio Minimal SD Bases), or galactose/raffinose with 10μg/ml doxycycline (Fisher Scientific BP26531) ...
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bioRxiv - Genetics 2022Quote: ... Colonies were grown on media lacking histidine and leucine (DO Supplement -His/-Leu, Takara Bio) to select for the presence of both vectors ...
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Orchestration of alternative splicing regulates bone marrow mesenchymal stem cells fate during agingbioRxiv - Cell Biology 2022Quote: ... bone sections were blocked in 5% bovine serum albumin (BSA) for 1 hour at room temperature and incubated with primary antibody to osteocalcin (Takara, M173) at 4°C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... and In-Fusion HD Enzyme (Takara, Japan).
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was generated from 1ng of input RNA using the SMART-Seq HT Kit (Takara 634455) at half reaction volume followed by Nextera XT (Illumina FC-131-1024 ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was generated from 1 ng of input RNA using the SMART-Seq HT Kit (Takara 634455) at half reaction volume followed by Nextera XT (Illumina FC-131-1024 ...
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bioRxiv - Cell Biology 2022Quote: ... The three fragments were fused using the in-fusion enzyme (Takara) into a pRetroX-TetOne-Puro (Takara ...
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bioRxiv - Cell Biology 2022Quote: ... reverse transcription and cDNA preamplification was performed using the SMARTer Ultra Low RNA Kit for the Fluidigm C1 System (Clontech) according to Fluidigm’s manual for mRNA sequencing on the C1 system ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were purchased from Takara for lentivirus production ...
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bioRxiv - Cancer Biology 2022Quote: ... filtered with 0.22 μm membrane and concentrated by 50-fold with Lenti-X Concentrator (Takara, #631232). All cells were transduced by spinoculation at 2000 rpm for 1 hour at room temperature supplemented with 8 μg/mL polybrene ...
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bioRxiv - Cancer Biology 2022Quote: ... Sequencing libraries were prepared using Thruplex DNA-seq kit (Takara, # R400675) and 10 μL of input material ...
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bioRxiv - Cell Biology 2022Quote: ... or into the BamHI/NotI site of pRetroX-Tight-puro vector (Clontech). The original vector of C-terminally HA-tagged Su9-TEV protease was gifted from Dr ...
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bioRxiv - Cell Biology 2022Quote: ... Retro-X-Tet-on Inducible Expression System (Clontech) was used according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: All plasmids construction was performed by PCR amplification (CloneAmp HiFi PCR Premix) using appropriate primers followed by Gibson assembly (In-Fusion HD Cloning system, Clontech) into the EcoRI site of pLVX-puro vector (Clontech ...
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bioRxiv - Cancer Biology 2022Quote: ... using SYBR Green PCR Master Mix (Takara), and transcript levels were normalized to actin as an internal control ...
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bioRxiv - Cancer Biology 2022Quote: ... Retroviruses corresponding to different CAR constructs were transduced into activated T cells in retronectin-coated plates (Takara). Cultures were allowed to expand for 10 days in their media supplemented with 300 IU ml-1 IL-2 ...
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bioRxiv - Cell Biology 2022Quote: ... anti-DsRed (TaKaRa, 1:200), anti-F-actin (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... anti-DsRed (TaKaRa, 1:10000), anti-actin (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... Viral supernatant was harvested 48 h after transfection and added to 6 well plates that had been precoated with 15 μg/ml RetroNectin (Takara Bio Inc.) and blocked with 2% BSA ...
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bioRxiv - Cell Biology 2022Quote: ... The yeast two-hybrid assays were performed according to the Matchmarker Gold Yeast two-hybrid system manual from Clontech. Different variations of the constructs were co-transformed by the polyethylene glycol/ lithium acetate method into the AH109 yeast strain and selected on SD/-Leu-Trp plates ...
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bioRxiv - Cell Biology 2022Quote: ... The RT kit was obtained from Takara Bio USA (Moutain view ...
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bioRxiv - Cell Biology 2022Quote: ... prior to adding 500 nM AP21967 (Takara) to the medium 30 min before imaging to induce self-assembly of nanocages ...
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bioRxiv - Cell Biology 2022Quote: ... into the EcoRI site of pLVX-puro vector (Clontech) or into the BamHI/NotI site of pRetroX-Tight-puro vector (Clontech) ...
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bioRxiv - Cancer Biology 2022Quote: ... then cloned into the doxycycline inducible pLVX-pTetOne-puro vector (Takara Bio) using In-Fusion HD (Takara Bio ...
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bioRxiv - Cancer Biology 2022Quote: ... using In-Fusion HD (Takara Bio) following the manufacturer’s manual ...
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bioRxiv - Cancer Biology 2022Quote: ... The HAND2 mutant (HAND2mut) open reading frame was cloned into the doxycycline inducible pLVX-pTetOne-puro vector (Takara Bio) using In-Fusion HD (Takara Bio ...
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bioRxiv - Cancer Biology 2022Quote: ... using In-Fusion HD (Takara Bio) following the manufacturer’s manual ...
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bioRxiv - Cell Biology 2022Quote: ... After being treated with DNase I (Takara Bio), the synthesized guide RNA was purified using the RNA Clean & Concentrator Kit (Zymo Research) ...
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bioRxiv - Cell Biology 2022Quote: ... Viral titers were measured using the Adeno-X rapid titer kit (Clontech) following manufacturer protocol.
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bioRxiv - Cell Biology 2022Quote: ... concentrated down 10 times using the Lenti-X Concentrator (631232; Takara Bio). Supernatants were kept at −80°C prior to being directly applied to target cells which were subsequently spun at 700 xg for 1 h at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... JK6L myeloma cells were a kind gift from Jonathan Keats (Translational Genomics Research Institute, USA) and Lenti-X™ 293T cells were obtained from Takara Bio (632180) ...
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bioRxiv - Microbiology 2022Quote: The following plasmids were generated by subcloning polymerase chain-reaction (PCR) generated amplicons from the indicated oligonucleotides from Table 1 into pEFtak or pLEX vector using In-fusion recombinase (Takara) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... InFusion cloning (Takara Bio) was used to amplify the linc-mipep-FLAG-HA coding sequence and ligate with the vector ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were harvested three days after transfection and AAV2 was isolated using AAVpro Extraction Solution (Takara, Catalog #6235). Two million 797 cells with homozygous insertions (clones 2 or 4 ...
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bioRxiv - Cancer Biology 2022Quote: ... Recombinant AAV2 was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara, Catalog #632608). Three days after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... was purchased from TaKaRa (cat#632496).
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bioRxiv - Cell Biology 2022Quote: ... the MSCV-puro vector (Takara Bio USA, #634401) were used ...
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bioRxiv - Bioengineering 2022Quote: ... or by CloneAmp HiFi PCR Premix (Takara Bio). Random mutagenesis was performed using Error-Prone PCR (EP-PCR ...
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bioRxiv - Plant Biology 2022Quote: ... we fused proAtSPCH and MpSETA CDS fragments by PCR using PrimeSTAR GXL polymerase (Takara Bio). The resultant PCR fragment was subcloned into pENTR1A entry clones (Invitrogen ...
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bioRxiv - Zoology 2022Quote: ... and 2X KAPA HiFi Hot Start Ready Mix 15 μl (TaKaRa Bio Inc., Japan), via a procedure with a thermal instrument (Applied Biosystems 9700 ...
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bioRxiv - Biochemistry 2022Quote: ... A reaction volume of 25 μl containing 1XPrimeStar Max DNA Polymerase (Takara), 300 nM of forward and reverse primers and 100 ng of DNA ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was prepared from cortical samples from control and cKO animals (3 animals/group) by using RNAiso-plus kit (Takara) and according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Titer was assessed using Lenti-X p24 Rapid Titer Kit (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... pQCXIP encoding Etr1-HA (Clontech) and pCIG-B72 ...
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bioRxiv - Cell Biology 2022Quote: ... into the pLVX-neo vector (Clontech). The CIBN-CAAX plasmid was obtained from Addgene (Plasmid #79574 ...
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bioRxiv - Cell Biology 2022Quote: ... coli (Clontech 636763) via heat shock ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were harvested and AAV2 was isolated using AAVpro Extraction Solution (Takara, Catalog #6235). Two million NC797 cells were infected with 30ul of each adeno-associated virus (AAV2) ...
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bioRxiv - Neuroscience 2022Quote: ... double-stranded cDNA whole transcriptome library was synthesized from 1µg of total RNA with Takara Bio’s SMARTer Stranded Total RNA Sample Prep Kit – HI Mammalian (Takara Bio, Cat. No. 634876) and SMARTer RNA Unique Dual Index Kit (Takara Bio ...
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bioRxiv - Neuroscience 2022Quote: ... and SMARTer RNA Unique Dual Index Kit (Takara Bio, Cat. No. 634418). Library preparation included ribosomal RNA depletion ...