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Citations for Takara Bio :
7601 - 7650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: RNA samples extracted from female or male adults were reverse-transcribed via AMV Reverse Transcriptase XL (TaKaRa) using Oligo dT Adapter primers (Table S4) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 1.5 μL of ligated reactant (15 ng DNA) was amplified using TaKaRa Ex-Taq (TaKaRa) with the following conditions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 100 ng RNA extracted from 12 male or female pharate larvae was reverse-transcribed using PrimeScript II reverse transcriptase (TaKaRa) at 50°C for 30 min ...
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bioRxiv - Evolutionary Biology 2022Quote: ... total RNAs (100–300 ng) extracted from sex determined adults or pharate larvae (108 hpo) were reverse-transcribed using PrimeScript™ II Reverse Transcriptase (TaKaRa Bio Inc.) at 30°C for 10 min ...
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bioRxiv - Immunology 2022Quote: ... Ligated plasmids were then transformed into Stellar competent cells (Takara Clontech, 636763) and single clones were picked on the next day ...
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bioRxiv - Immunology 2022Quote: ... (both kind gifts from Pilar Gonzalo and Joaquin Teixidó (Bartolomé et al. 2009)) were cloned into the lentiviral backbone pLVX-CMV-IRES-zsGreen1 (Takara/Clontech #632187) by Gibson assembly (Gibson et al ...
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bioRxiv - Immunology 2022Quote: ... (both kind gifts from Pilar Gonzalo and Joaquin Teixidó (Bartolomé et al. 2009)) were cloned into the lentiviral backbone pLVX-CMV-IRES-zsGreen1 (Takara/Clontech #632187) by Gibson assembly (Gibson et al ...
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bioRxiv - Genomics 2022Quote: ... 1.51 million bacterial colonies were collected and the plasmids were extracted with NucleoBond Xtra Maxi EF kit (Takara Bio USA #740424). To insert a TmU DNA fragment containing the tracRNA (E/F ...
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bioRxiv - Immunology 2022Quote: ... The rest of the supernatant was heat-inactivated and concentrated using Retro-X concentrator (Takara Bio), following manufacturer’s recommendations and published protocols63,64.
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bioRxiv - Immunology 2022Quote: ... The rest of the supernatant was UV-inactivated and concentrated using Retro-X concentrator (Takara Bio), following manufacturer’s recommendations and published protocols63,64.
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bioRxiv - Immunology 2022Quote: ... cDNA was synthesized using the PrimeScript RT Reagent Kit (Takara) following manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... StellarTM cells (Takara) were transformed with the resulting plasmids ...
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bioRxiv - Genetics 2022Quote: SMART-Seq v4 Ultra Low Input Kit for Sequencing was used for full-length cDNA synthesis and amplification (Clontech, Mountain View, CA), and Illumina Nextera XT library was used for sequencing library preparation ...
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bioRxiv - Immunology 2022Quote: ... the PCR product was assembled into an EcoRI + XhoI digested pMSCV-neo (Takara Clontech) vector by using Gibson Assembly Master Mix (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... cDNAs were synthesized with PrimeScript RT Master mix (Takara) followed by adding template RNA ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The recombinant Irg1-like protein was enriched using cobalt TALON Metal Affinity Resin (Takara Bio #635501) with gentle shaking for 1 hour at 4 ℃ and washed twice by centrifugation for 3 minutes at 3500 rpm at 4 ℃ and resuspension in the washing buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Genomics 2022Quote: ... and post-capture PCR amplification was performed for 16-18 cycles using PrimeSTAR GXL Polymerase (Takara) at an annealing temperature of 60°C.
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bioRxiv - Genomics 2022Quote: ... PCR amplification was performed for 8-9 cycles using PrimeSTAR GXL Polymerase (Takara) at an annealing temperature of 60°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Yeast 2-hybrid library screening was conducted using the Matchmaker Gold Yeast Two-Hybrid System (Takara Bio USA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: A yeast two-hybrid prey cDNA library of Trichoplax whole animal RNA was prepared in the pGADT7- AD plasmid vector using the Make Your Own “Mate & Plate™” Library System (Takara Bio, California USA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The included restriction sites permitted cloning into both the pIRES2-EGFP and pEGFP-C1 mammalian expression vectors (Clontech), the former a bicistronic vector expressing enhanced green fluorescence protein (EGFP ...
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bioRxiv - Genomics 2022Quote: ... coli by using a NucleoBond Xtra BAC kit (Takara, 740436.25) for delivery into mESCs ...
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bioRxiv - Genomics 2022Quote: ... and converted into cDNA (1st strand cDNA synthesis kit, Takara). Real time PCR was carried out using the LC480 Thermocycler (Roche ...
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bioRxiv - Developmental Biology 2022Quote: ... 500 ng of total RNA was reverse-transcribed into cDNAs using the PrimeScript RT Reagent Kit (TaKaRa, RR037A) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... and quantified by the AAVpro Titration Kit (Takara, 6233). gRNA AAV viruses (1×103 MOI ...
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bioRxiv - Genomics 2022Quote: ... colonies were picked into 96-well plates and a small proportion of cells from each colony were used for DNA extraction using Quick Extract solution (Epicenter) and direct PCR with Prime STAR® GXL DNA Polymerase (Clontech). PCR amplicons were sequenced by Sanger to verify the deletion (SFigure 17).
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bioRxiv - Developmental Biology 2022Quote: ... Gene expressions was obtained by RT-qPCR (PrimeScript transcriptase, Takara, SYBRgreen) and normalized with Pfaffle equation using two housekeeping genes (rpl7 ...
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bioRxiv - Genomics 2022Quote: ... reverse transcription of the RNA using a primer designed to the constant region of the barcoded adaptor with addition of an adapter to the 3’ end of the cDNA by template switching using SMARTScribe (Clontech) as described (Zhu et al. ...
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bioRxiv - Genomics 2022Quote: ... Total RNA from SARS-CoV-2 infected lung or trachea was subjected to one-step real-time reverse transcription PCR using One-step PrimeScript RT-PCR kit (Takara, RR064B). Multiplex PCR was performed to detect SARS-CoV-2 nucleocapsid gene and mouse Actb gene ...
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bioRxiv - Genetics 2022Quote: ... and the constructs were co-transformed into yeast strain Y2HGold (Takara). Co-transformants were selected on SD plates containing minimal supplements without leucine and tryptophan (SD/-Leu/-Trp ...
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bioRxiv - Genetics 2022Quote: ... Produced lentiviruses were concentrated 100x with Lenti-X Concentrator (#631232, Takara) to increase virus concentration.
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bioRxiv - Genetics 2022Quote: ... and then reverse transcribed RNA to get cDNA using PrimeScript RT reagent kit (Takara, Osaka, Japan). Supplementary Table 1 lists the primers used to construct minigene vectors and to detect alternative splice sites.
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bioRxiv - Genomics 2022Quote: ... These guides were individually cloned into pAAV-U6-sasgRNA-CMV-mCherry-WPREpA (92) at the BstXI and XhoI restriction enzyme sites using the In-Fusion (Takara Bio, 638910) cloning methods as described in (92) ...
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bioRxiv - Genomics 2022Quote: ... rAAV-9 serotype virons were produced by transfecting AAVpro 293T cell (Takara, 6322723) with pCMV-sadCas9-KRAB (Addgene ...
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bioRxiv - Immunology 2022Quote: ... 25 μg of the retroviral expression vector (pLZRS-human codon-optimized Bcl6-P2A-human codon-optimized BclxL-IRES-GFP) and 5 μg of the envelope vector (p10A1; Clontech) were transfected into GP2-293 cells using Lipofectamine 3000 (ThermoFisher ...
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bioRxiv - Immunology 2022Quote: ... GP2-293 retroviral packaging cells and the p10A1 envelope vector were purchased from Clontech. Lipofectamine 3000 was purchased from ThermoFisher and Polybrene was purchased from Sigma ...
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bioRxiv - Immunology 2022Quote: ... for continuous fluorescence detection in a total volume of 10 μL of cDNA/control and gene-specific primers using SYBR Premix Ex Taq (TaKaRa Bio). The mRNA levels of the target genes were normalized relative to those of Gapdh using the following formula ...
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The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residencybioRxiv - Immunology 2022Quote: ... RetroNectin (12 µg/mL, Takara Bio, Saint-Germain-en-Laye, France) was coated and the previously mentioned cytokines were added in combination with polybrene (8 µg/mL ...
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bioRxiv - Immunology 2022Quote: ... IGK and IGL 5’RACE AIRR-seq libraries were generated using the SMARTer Mouse BCR Profiling Kit (Takara Bio ...
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bioRxiv - Immunology 2022Quote: ... 200 ng was removed for NGS library preparation (SMART-Seq v4 Ultra Low Input RNA, Takara), Illumina adapter addition ...
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bioRxiv - Microbiology 2022Quote: ... We cloned the amplicons using the In-Fusion® cloning method (Takara Bio) into pET28b vectors for expression in Escherichia coli with two 6x Histidine tags (6xHis-tags ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid pTAP-AFAP was constructed by cloning the gene fragment encoding AFAP-streptavidin-binding peptide-3xFLAG tag fusion protein (AFAP-SBP-3xFLAG, shorted as AFAP-SF) into mammalian expression vector pIRESpuro3 (Takara). SBP is a peptide with the length of 38 amino acids (Wilson et al. ...
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bioRxiv - Microbiology 2022Quote: ... which expresses GFP was from Clontech Laboratories ...
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bioRxiv - Immunology 2022Quote: ... the variant HXP-S were inserted into the pNDV_LS/L289A rescue plasmid (between P and M genes) by in-Fusion cloning (Clontech, CA, USA). The recombinant product was transformed into MAX Efficiency™ Stbl2™ Competent Cells (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Samples showing the correct size band for heavy and light chain amplification in the respective wells were subsequently cloned into pAb20-hCHIgG1 (Synbio; for heavy chain) and pAb20-hCK (Synbio; for light chains) through in-fusion cloning (Takara). The ligated product was transformed to DH5-α competent cells ...
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bioRxiv - Immunology 2022Quote: ... cells were mixed on retronectin (Takara Bio)-coated plates with CER lentivirus at a MOI of ∼ 20 followed by spinoculation for 2 hours at 1,200xg ...
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bioRxiv - Immunology 2022Quote: ... cDNA was synthesized to a total of 500ng RNA in a 20 μL system using PrimeScript™ RT Reagent kit (TAKARA, #RR036A). q-PCR were performed on anlytik jena (qTOWER 2.0 ...
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bioRxiv - Immunology 2022Quote: ... Lenti-XTM 293T cells (Takara Bio) in DMEM media containing 10% FBS and 100 U/ml Pen/Strep were transfected using the standard calcium phosphate method with 15 μg of the CER transfer vector ...
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bioRxiv - Immunology 2022Quote: ... and amplified and reverse transcribed into cDNA using the SMART-seq protocol (Takara), followed by a quality control and library preparation ...
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bioRxiv - Microbiology 2022Quote: ... Seven Ns were added into the primers as the barcode for barcoded-subamplicon sequencing during the first round PCR (PrimeSTAR Max DNA Polymerase, Takara Bio ...