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Citations for Takara Bio :
7651 - 7700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... HEK-293T cells were transfected with calcium phosphate (Clontech) in T75 flasks using a total of 30 μg plasmid DNA ...
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bioRxiv - Cell Biology 2022Quote: ... which was used to screen a Mate & Plate™ Library-Universal Mouse (Normalized) (Clontech, Mountainview, CA; Cat#: 630482) using the stringent protocol according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... RT-qPCR was performed using Real Time System TP800 (TAKARA bio systems), according to manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: ... mRNA was extracted and prepared with SMARTer Universal Low Input RNA Kit for Sequencing (Takara) for 100 bp paired-end sequencing on the NovaSeq 6000 by the Integrated Genomics Organization at MSKCC ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from cells by TRIzol and an RNA extraction kit (MiniBEST Universal RNA Extraction Kit, RR820A, Takara, China). Total RNA (1 μg ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA (1 μg) was reverse transcribed by a reverse transcription kit (RR047A, Takara, China). Real-time quantitative PCR was performed on ABI 7500 system (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... SMART-Seq v4 Ultra Low Input Kit for Sequencing was used for full-length cDNA synthesis and amplification (Clontech, Mountain View, CA), and Illumina Nextera XT library was used for sequencing library preparation ...
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bioRxiv - Immunology 2022Quote: ... and reverse transcribed into cDNA using PrimeScript RT Reagent Kit (Takara) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Total RNA libraries were prepared using the SMARTer Stranded Total Kit v3 (Takara). Extracted RNA and libraries were assessed for quality on a TapeStation 2200 instrument (Agilent) ...
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bioRxiv - Immunology 2022Quote: ... PCR amplicons were purified from 1% agarose gels following electrophoresis by column purification (Takara) and submitted for sanger sequencing through Genewiz (Primer ...
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bioRxiv - Immunology 2022Quote: ... The viral RNA copy number was standardized with a Positive Control RNA Mix (2019-nCoV) (Takara, Cat#XA0142). Relative viral copy was calculated as viral RNA copy number obtained by virus-infected targets without T cells normalized to 1.
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bioRxiv - Immunology 2022Quote: ... Primer/Probe N2 (2019-nCoV) (Takara, Cat# XD0008) were used as follows ...
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bioRxiv - Immunology 2022Quote: ... Real-time RT-PCR analyses for viral RNA copy number was carried out with One Step PrimeScript™ III RT-qPCR Mix (Takara, Cat# RR600B) and reactions were performed by using LightCycler® 96 System (Roche Diagnostics GmbH ...
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bioRxiv - Immunology 2022Quote: ... and reverse transcribed to cDNA with RNA to cDNA EcoDry Premix (Random Hexamers) (Takara/Clonetech). qPCR mastermix was made with Eukaryotic 18S rRNA Endogenous Control (ThermoFisher) ...
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bioRxiv - Immunology 2022Quote: ... and first strand cDNA was synthesized with Reverse Transcriptase M-MLV (TaKaRa). Real-time PCR was performed using Hieff® qPCR SYBR Green Master Mix (No Rox ...
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bioRxiv - Immunology 2022Quote: ... placed on 20 μg/mL RetroNectin (Clontech, Mountain View, CA, USA)-coated 12-well plates ...
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bioRxiv - Evolutionary Biology 2022Quote: ... magnanima was assessed by PCR amplifying a cifB-like gene (Extended Data Table 3) in the WOwHm-t76 region with the Emerald Amp Max Master mix (TaKaRa) at 94°C for 3 min ...
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bioRxiv - Cell Biology 2022Quote: ... Reactions were performed in a reaction mixture consisting of SYBR Premix Ex Taq (Takara, Tokyo, Japan) and primers with cDNA in a volume of 25 mL (Table3) ...
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bioRxiv - Cell Biology 2022Quote: ... The reverse transcription reaction was performed using ExScript RT Reagent Kit (Takara, Tokyo, Japan). The primers used in this study were as follows:
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bioRxiv - Developmental Biology 2022Quote: ... each clone was treated with Dox (Takara) at 0 ...
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bioRxiv - Cell Biology 2022Quote: Library preparation was performed using a SMARTer Stranded Total RNA-Seq Kit v2 (TaKaRa, 634417) following the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2022Quote: ... FcγRIIA cDNA was cloned into a pEGFP-N vector (Takara Bio USA, CA). FTractin cDNA was fused with Halo tag in pTriEx-4 vector (Millipore Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... FcγRIIA-EGFP or Lifeact-mCherry was cloned into pAdenoX-Tet3G (Takara Bio), and adenovirus was prepared according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... RNA (1 ng) was amplified using SMARTer library Ultra Low cDNA v4 kit (Takara Bio, Mountainview, CA). Sequencing cDNA libraries were preparing using a NexteraXT DNA library prep kit (Illumina ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 RNA quantification was performed by RT-qPCR targeting the S gene of SARS-CoV-2 using One Step PrimeScript RT-PCR Kit (Takara) with the following SARS-CoV-2 specific primers and probes ...
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bioRxiv - Immunology 2022Quote: ... Cells were lysed using Takara Bio Single Cell Lysis Buffer containing a recombinant RNase inhibitor (Takara Bio Inc.) then snap frozen ...
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bioRxiv - Immunology 2022Quote: PCR reactions were carried out with the Primestar taq polymerase (Takara) for 30 cycles of denaturation (95 °C) ...
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bioRxiv - Cell Biology 2022Quote: ... and that for the GFP vector is pEGFP-C1 (Takara Bio Inc).
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bioRxiv - Cell Biology 2022Quote: Every protein expressed in mouse rods was also cloned into pEGFP-N1 (Clontech) for expression in AD293 cells using the AgeI and NotI cloning sites within the vector to replace EGFP with the tagged proteins ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from cells treated with either buffer or Mindin for 16-hours using TRIzol Reagent (TaKaRa, Thermo Fisher Scientific) and cDNA was synthesised using Superscript III (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: The ChIP library was prepared using ThruPLEX DNA-seq kit (Takara Bio), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted using RNAiso Plus (Takara Bio, Shiga, Japan). RNA (1 μg ...
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bioRxiv - Cell Biology 2022Quote: ... HEC1 and BUBR1 were amplified from Human testis cDNA (Marathon cDNA; Takara Bio Inc.) using Pfu polymerase (Promega) ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA was purified using the NucleoSpin Gel & PCR Clean-up Kit (Takara, 740986.20) and 100 bp paired end reads sequenced using Illumina NovaSeq at the Integrated Genomics Operation at MSKCC ...
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bioRxiv - Cancer Biology 2022Quote: ... amplified from the linear puro marker (Cat. No. 631626, Takara-Bio), was inserted using the In-fusion HD cloning kit (Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... The Lenti-X™ 293T cell line was purchased from Takara-Bio (Cat ...
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bioRxiv - Cancer Biology 2022Quote: PrimeSTAR max polymerase (Cat. No. R045A, Takara-Bio) or KOD one polymerase (Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-H3K27me1 (1:1000, Takara, #MABI0321-100I), anti-H3K27ac (1:4000 ...
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bioRxiv - Cancer Biology 2022Quote: ... The 1st PCR was carried out with PrimeSTAR® Max DNA polymerase (TaKaRa Bio Inc.), targeting the region where the mutations were induced by the error-prone PCR ...
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bioRxiv - Cancer Biology 2022Quote: ... Base substitutions were induced in the pMXs vector with the PrimeSTAR® Mutagenesis Basal Kit (TaKaRa Bio Inc.) before transfection into Plat-E cells to create ALK-TKIs resistant mutants.
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bioRxiv - Cancer Biology 2022Quote: ... 2nd PCR was performed with TaKaRa Ex Taq® Hot Start Version (TaKaRa Bio Inc.). The PCR products were purified with AMPure XP (BECKMAN COULTER) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and the amplicon of each NeNaC probe was amplified using the Advantage 2.0 Polymerase (Takara Bio, Japan) from cDNA of different life stages of N ...
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bioRxiv - Cell Biology 2022Quote: ... Human embryonic kidney cells Lenti-X 293T (Clontech) were cultured in DMEM media supplemented with 10% FBS (Gibco) ...
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bioRxiv - Cancer Biology 2022Quote: ... After 48 hours supernatants were collected and employed to LAL-B cells in Retronectin (Takara Bionic Otsu, Shiga 520-2193, Japan) pre-coated no tissue culture 24-well plates (Falcon ...
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bioRxiv - Cancer Biology 2022Quote: U-2 OS Tet-On cells were purchased from Clontech (#630919). MDA-MB-453 cells were purchased from the American Type Culture Collection (ATCC ...
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bioRxiv - Cell Biology 2022Quote: ... to the pEGFP-C1 (Clontech) vector backbone in between the AgeI/HindIII restriction sites ...
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bioRxiv - Cell Biology 2022Quote: ... achieving 10x concentrated preparations of which the lentiviral titer was determined using Lenti-X GoStix Plus (Takara, 631280). Lentiviral preparations were aliquoted and stored at -80°C until use.
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bioRxiv - Developmental Biology 2022Quote: ... was used to purify RNA and 500 ng RNA was used for reverse transcription with a Prime Script RT Reagent Kit (RR037A; Takara). Quantitative PCR was performed using a TB Green Premix DimerEraser Kit (RR091A ...
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bioRxiv - Genetics 2022Quote: ... 1 µg of total RNA was used for reverse transcription using PrimeScript RT Reagent Kit (Cat#RR037A, TaKaRa, Japan). Quantitative polymerase chain reaction (qPCR ...
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bioRxiv - Genetics 2022Quote: ... Physical titer was determined using the Lenti-X p24 Rapid Titer Kit (Takara, 632200) and granule cell precursors were transduced at an estimated MOI of ∼4.