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Citations for Takara Bio :
7551 - 7600 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and then ten nanograms of total RNA was used as input for cDNA synthesis using the Clontech SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were harvested and further concentrated using a Lenti-X Concentrator (Takara Bio Inc., Otsu, Japan). Naive CD4+ T cells were transduced with lentiviruses and polybrene (8 ug/mL ...
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bioRxiv - Immunology 2023Quote: ... Total RNA was normalized prior to oligo-dT capture and cDNA synthesis with SMART-seq v4 kit (Takara). The resulting cDNA was quantitated using a Qubit 3.0 fluorometer ...
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bioRxiv - Immunology 2023Quote: ... A total of 100 ng isolated RNA was used for cDNA synthesis using PrimeScript High Fidelity RT-PCR Kit (Takara, R022B). RT-qPCR was performed using TB Green® Premix Ex Taq™ (Tli RNase H Plus ...
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bioRxiv - Plant Biology 2023Quote: ... and RNA-seq analysis (150 bp PE reads, Novaseq 6000 platform, by Takara Bio; Table S2) as described above.
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bioRxiv - Plant Biology 2023Quote: First-strand cDNA was synthesized from purified total RNA using PrimeScript RT Master Mix (Perfect Real Time; Takara Bio, Shiga, Japan) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: The WST-1 assay (Takara) was performed on HepG2 cells and Huh7 cells to assess the cellular cytotoxicity of Tecomella undulata ...
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bioRxiv - Biochemistry 2023Quote: Khc FL for in vitro studies was cloned with a HisSUMO-SNAP-3C-tag into MCS1 between BamHI and HindIII sites of the pFastBacDual vector by InFusion cloning (Takara). The Khc mutant constructs used for in vitro studies were generated by site-directed mutagenesis of the Khc FL plasmid using primers that either inserted a stop codon after aa 910 (Khc910 ...
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bioRxiv - Cell Biology 2023Quote: ... Separate populations were stably-transfected to express MLCK1-EGFP constitutively and mCherry-Ig3 inducibly via a Tet-on3G system (Clontech). Cells were maintained in high-glucose Dulbecco’s modified Eagle’s medium (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... BsrGI digested and ligated into a likewise digested C1 (Clontech) backbone ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant constructs were transformed into Y1H gold strain (Takara). The transformed yeast strains were grown on SD/-Leu and SD/-Leu/AureobasidinA media (Takara ...
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bioRxiv - Plant Biology 2023Quote: The PCR amplified promoter sequence of AtPHT1;5 gene was cloned into pAbAi vector (Takara) between the SacI and KpnI RE sites following manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... and AtMYB62 genes were amplified by PCR using gene specific primers and cloned into pGADT7-Rec vector (Takara). The recombinant constructs were transformed into Y1H gold strain (Takara) ...
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bioRxiv - Plant Biology 2023Quote: ... and Y1H analysis was performed using Matchmaker® Gold Yeast One-hybrid screening system (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... The transformed yeast strains were grown on SD/-Leu and SD/-Leu/AureobasidinA media (Takara) and Y1H analysis was performed using Matchmaker® Gold Yeast One-hybrid screening system (Takara ...
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bioRxiv - Genomics 2023Quote: ... Primers were designed with around 15 bp of overlapping sequence to ensure proper circularization with the In-Fusion HD cloning plus kit (Takara #638909). Mach1 competent cells (Thermo Fisher Scientific #C862003 ...
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bioRxiv - Microbiology 2023Quote: ... The product was circularized with In-Fusion HD Cloning (Takara Bio) as per the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... which was reverse transcribed to cDNA by Takara PrimeScript RT reagent kit (RR0036A ...
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bioRxiv - Molecular Biology 2023Quote: ... doxycycline (Clontech 631311), hygromycin B Gold (InvioGen) ...
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bioRxiv - Neuroscience 2023Quote: ... 1 million Lenti-X HEK293T cells (Takara Bio, cat. no. 632180) were seeded in 2 mL DMEM (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... and 2 μg/ml doxycycline (Clontech, cat. no. 631311). Neuronal induction media was changed once a day for 2 more days ...
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bioRxiv - Molecular Biology 2023Quote: ... retrotranscribed using PrimeScript Reagent Kit (Takara) and amplified by RT-qPCR using PowerUp SYBR-Green MasterMix (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: Human neurons were transfected on DIV28 with pEGFP-C1 (0.3 μg/well, Clontech) plus vectors expressing mCherry ...
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bioRxiv - Biochemistry 2023Quote: ... The digested XLone vector was then fused with the insert of the respective constructs by in-fusion cloning method according to the manufacturer’s instructions (Takara Bio Inc.). The resulting products from the respective in-fusion cloning reactions were transformed into stbl3 One-Shot competent cells according to the manufacturer’s instructions (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... For amplification of DNA sequences for cloning and NGS the PrimeSTAR Max DNA polymerase (Takara Bio Inc.) and for colony PCRs the OneTaq Quick-Load Master Mix Polymerase was used (NEB) ...
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bioRxiv - Biochemistry 2023Quote: ... and inserted into a modified pEGFP-N1 vector (Clontech) in which the downstream EGFP gene was deleted and an HA tag was added at the C-terminus ...
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bioRxiv - Biochemistry 2023Quote: ... the PrimeSTAR Max DNA Polymerase (Takara Bio Inc.) was used according to the manufacturer’s recommendation with a 25 µl reaction volume ...
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bioRxiv - Biochemistry 2023Quote: ... and reverse transcribed according to the manufacturer‘s protocol (Takara). qRT-PCR was carried out using SYBR Premi qRT-PCR ExTaq™ II (Takara ...
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bioRxiv - Biochemistry 2023Quote: ... Lentivirus was concentrated by adding 333 μL Lenti-X concentrator (Takara, cat# 631231) per mL of viral supernatant ...
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bioRxiv - Biochemistry 2023Quote: The retroviral titre calculation was done using the Retroviral titration kit from Takara Biosciences (Retro-X™ qRT-PCR Titration Kit 631453 ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was collected and concentrated using Retro-X-Concentrator from Takara Biosciences (Takara #631455 ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with 125 μL of TALON cobalt resin (Takara Bio ...
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bioRxiv - Neuroscience 2023Quote: ... inserting an in-frame 15bp flexible linker sequence (encoding the amino acids GGGGA) followed by EGFP at the C-terminus using Infusion cloning (Clontech). For the Tg(NBT:ap2s1-gfp ...
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bioRxiv - Cancer Biology 2023Quote: ... 1-st PCR (PCR 1) was performed using Titanium Taq DNA Polymerase (# 639209, Takara Bio, CA, USA). Separation of the PCR products from primers and gel purification was done by QIAquick PCR & Gel Cleanup Kit (Qiagen ...
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bioRxiv - Systems Biology 2023Quote: ... Genomic DNA was extracted from cell pellets using the Machery Nagel NucleoSpin Blood Kit (Midi, Clontech Cat ...
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bioRxiv - Systems Biology 2023Quote: ... Genomic DNA was extracted from cell pellets using the Machery Nagel NucleoSpin Blood Kit (Midi, Clontech Cat. #740954.20; XL Maxi, Clontech Cat. #740950). The detailed protocol is available at http://www.broadinstitute.org/rnai/public/resources/protocols.
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bioRxiv - Biochemistry 2023Quote: ... HEK293T cells were transiently transfected with the expression control vector pCMV-TRE3G (Clontech, Mountain View, CA, USA) and the coexpression construct pTRE3G-BI/GPR83-LgBiT:SmBiT-ARRB2 that encodes a C-terminally LgBiT-fused human GPR83 (LgBiT-GPR83 ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was collected and concentrated using Retro-X-Concentrator from Takara Biosciences (Takara #631455) overnight at 40C ...
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bioRxiv - Biochemistry 2023Quote: ... GFP (JL-8, Clontech) or Vinculin (7F9 ...
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bioRxiv - Biochemistry 2023Quote: ... GFP expression was verified using immunoblotting using GFP (JL-8, Clontech) and α-tubulin (CP06 ...
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bioRxiv - Cell Biology 2023Quote: In-fusion cloning was performed using the In-Fusion HD EcoDry Cloning Plus kit (Takara), and standard cut-and-paste cloning using T7 DNA ligase (New England Biolabs) ...
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bioRxiv - Genomics 2023Quote: ... cDNA was amplified using SYBR Premix ExTaq (Takara, Madison, WI, USA) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: pET28a-Halotag-CNA35 was created using InFusion HD Assembly (Takara) by replacement of the eGFP coding sequence within pET28a-eGFP-CNA3546 (Addgene ...
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bioRxiv - Plant Biology 2023Quote: Binary vectors for agrobacterium-mediated transformation were constructed using the backbone of pRI-201 (TaKaRa Bio) or pGWB (Nakagawa et al. ...
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bioRxiv - Biochemistry 2023Quote: ... Affinity purification of Ypq1-3C-GFP-8xHis was done by incubating the supernatant with 3 mL TALON cobalt resin (635502; TaKaRa Bio) pre-equilibrated with Column buffer (20 mM HEPES-KOH (pH 7.2) ...
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bioRxiv - Biochemistry 2023Quote: ... Soluble material was incubated with His60 Superflow resin (Clontech) for 60 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Viral titer was quantified using the Lenti-X™ qRT-PCR Titration Kit (Takara Bio, #631235), according to manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... cells were exchanged with fresh media containing B/B homodimerizer (500nM) (Takara Bio, #635059) and/or Baf-A1 (100nM ...
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bioRxiv - Biochemistry 2023Quote: ... MBP-Hua2(K221WY -AWY)NT-FLAG and MBP-Hua2(G183KV-AAV)NT-FLAG were constructed by introducing point mutations using In-Fusion HD Cloning (Takara Bio USA, Inc) with the primers listed in Table 1 ...
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bioRxiv - Genetics 2023Quote: ... and cloned these into expression vectors using Infusion cloning (In-Fusion HD Cloning Plus, Cat. 638911, Takara Bio USA.)