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Citations for Takara Bio :
7451 - 7500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... 0.25 μL Recombinant RNase Inhibitor (Clontech), 2 μL Betaine (5 M Sigma) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... was cloned into a bicistronic lentiviral expression vector pLVX-EF1α-IRES-mCherry (Clontech) creating pLVX-EF1α-IRES-mCherry-NLuc ...
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bioRxiv - Neuroscience 2022Quote: The pEGFP vector (ClonTech, Mountain View, CA) harboring synhDAT ...
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bioRxiv - Neuroscience 2022Quote: ... nuclei were unfixed and isolated as described above except that tissue was first dounced in 1mL lysis buffer with 400U RNase inhibitor (Takara, 2313A). Following ultracentrifugation ...
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bioRxiv - Molecular Biology 2022Quote: HEK-293 Tet-Off (HEK-293TO) cells (Clontech) and HEK-293 Flp-In T-REx cells (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: Rps29 5’UTR and coding region cDNAs were synthesised using SMART™ RACE cDNA Amplification Kit (Clontech Laboratories Inc. USA). PCR was performed using gene-specific primers (40S 5’ RACE R ...
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bioRxiv - Molecular Biology 2022Quote: ... These three fragments were ligated using In-fusion (Takara Bio Inc, Shiga, Japan), and the resultant plasmid was named psfRps29_WT.
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bioRxiv - Molecular Biology 2022Quote: ... and deoxyribonuclease I (DNase I, 5 units/mL, Takara, Shiga, Japan) for 15 min ...
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bioRxiv - Molecular Biology 2022Quote: cDNA was prepared from EV RNA using the Single Cell RNA kit SMART-Seq_V4 Ultra Low Input RNA Kit (634890, Takara, Mountain View, CA, USA) according to manufacturer instructions using 1ng input ...
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bioRxiv - Molecular Biology 2022Quote: ... poly-A libraries were prepared using the SMARTer ThruPLEX DNA-seq Kit (R400675, Takara, Mountain View, CA, USA). Each library was normalized to 1.5 nM ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal amounts of RNA samples (200 ng) were used to generate complementary DNA using a PrimeScript RT reagent kit (TaKaRa Bio) with oligo-dT and random primers ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was amplified by PCR in separate 25 μL reactions each containing 2 μL of cDNA for 11-13 cycles using 0.5 μL (2.5 U) LA Taq (Takara, Cat# RR002M) with P5-3’ and miRCat-PCR-2 oligos at an annealing temperature of 58°C ...
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bioRxiv - Neuroscience 2022Quote: ... RNAseq libraries were prepared using the SMARTer (Takara) Low input RNA kit for Illumina sequencing ...
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bioRxiv - Neuroscience 2022Quote: ... 40U/mL of RNAse inhibitor (Takara: 2313B)] ...
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bioRxiv - Neuroscience 2022Quote: ... and the Thermal Cycler Dice Real-Time System (Takara Bio).
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bioRxiv - Immunology 2022Quote: ... and then cryopreserved in CellBanker 1plus (TAKARA Bio, Kusatsu, Japan) at –80°C until use.
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bioRxiv - Biochemistry 2022Quote: ... PrimeScriptTM 1st Strand cDNA Synthesis (Takara), sequence retrieval by RACE ...
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bioRxiv - Bioengineering 2022Quote: ... Reverse transcription to obtain cDNA was performed according to the instructions of the PrimeScript 1st Strand cDNA Synthesis Kit (Takara Biomedical Technology (Beijing) Co. ...
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bioRxiv - Microbiology 2022Quote: ... and InFusion (Takara). Silent mutations at the gRNA targeting site were also introduced using InFusion ...
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bioRxiv - Neuroscience 2022Quote: ... 0.5 U/μL RNAse inhibitor (Takara, 2313A) and 0.5 % biocytin (Sigma B4261) ...
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bioRxiv - Cancer Biology 2022Quote: Lysis plates were prepared by dispensing 0.4 µL lysis buffer (0.5 U Recombinant RNase Inhibitor (Takara Bio, 2313B), 0.0625% Triton™ X-100 (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... cell cycle indicator (FUCCI) was cloned from the pRetroX-SG2M-Red Vector (Takara Bio, 631465) and added to the pB-IUE cocktail ...
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bioRxiv - Molecular Biology 2022Quote: ... and Human GABRB3 (IMAGE ID 3871111, Source BioScience) were used to obtain N-terminal GST fusions in pGEX-KG (Clontech) or N-terminal FLAG fusions in pJEN1 (pcDNA3 derived ...
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bioRxiv - Cancer Biology 2022Quote: ... to yield pMXs-Neo-hMPI by using In-Fusion HD Cloning Kit (639648, Clontech), in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... pneumoniae K2 capsule cluster from ATCC 43816 was cloned in two separate sections using In-Fusion Snap Assembly (Takara Bio USA). The genes from wcuF through wzx were cloned into PCR-linearized pBBR1MCS3 (48) ...
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bioRxiv - Microbiology 2022Quote: ... Site directed mutagenesis was performed using In-Fusion Cloning Kit (Takara). Primers used for mutagenesis can be found in Table S2 and purchased from IDT DNA Technologies (Coralville ...
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bioRxiv - Microbiology 2022Quote: ... and pLVX-EF1α-IRES-Puro was purchased from Clontech Laboratories (plasmid #631988).
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bioRxiv - Genetics 2022Quote: ... and concentrated with Lenti-X Concentrator (Takara, 631231) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... and the cDNA was synthesized using the PrimeScript™ RT Reagent Kit with gDNA Eraser (Takara) as recommended by the manufacturer with an oligo dT used instead of random primers ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR-amplified DNA fragments were assembled and cloned into pET-in situ Ble plasmid by recombination (InFusion, Clontech) and the resulting cell line was named ABbin situ ...
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bioRxiv - Developmental Biology 2022Quote: ... The qRT-PCR was performed with the cDNA using the SYBR-green stain (Takara). Primers used for qPCR are listed below.
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bioRxiv - Cell Biology 2022Quote: ... Human embryonic kidney cells Lenti-X 293T (Clontech) were cultured in DMEM media supplemented with 10% FBS (Gibco) ...
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bioRxiv - Developmental Biology 2022Quote: ... The picked endogenous IHCs and iOHCs were immediately subjected to reverse-transcription and cDNA amplification using a Smart-Seq HT kit (Cat# 634437, Takara). The post-amplified cDNA (1 ng ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA synthesis was performed with SMART- Seq v4 Ultra Low Input RNA Kit (Clontech cat. 634888) according to the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2022Quote: ... and rabbit anti-dsRed (Takara, 632496) (1:200 ...
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bioRxiv - Genetics 2022Quote: ... The sequence of amplified MYSM1 cDNA was verified by Sanger sequencing before subcloning into the pLVX overexpression vector using the In-Fusion cloning kit (Takara). Q5 site directed mutagenesis kit was used to make H656R mutant.
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bioRxiv - Genetics 2022Quote: ... then washed in water and re-suspended in 125-250mL -leu - his galactose liquid culture (Takara Bio Minimal SD Bases ...
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bioRxiv - Genetics 2022Quote: ... then washed in water and re-suspended in 125-250mL -leu - his galactose liquid culture (Takara Bio Minimal SD Bases, Takara Bio DO Supplement -His/-Leu). Cultures were grown to saturation ...
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bioRxiv - Developmental Biology 2022Quote: ... Retrovirus supernatant or medium containing virus particles was harvested at day 2 post transfection and concentrated by Retro-Concentrator (Clontech) solution ...
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bioRxiv - Developmental Biology 2022Quote: ... Trim33 PCR was performed using LA Taq polymerase (Takara) and the following primer pair:
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bioRxiv - Developmental Biology 2022Quote: ... 5’ and 3’ RACE ready cDNA was generated from a pooled female pupal RNA sample and also from a pooled adult female ovary sample (RNA extracted using RNeasy MinElute kit, RACE conducted using SMARTer 5⍰/3⍰ RACE kit - Takara Bio, Kyoto, Japan). Visible bands were cloned using the NEB PCR cloning kit (New England Biolabs ...
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bioRxiv - Developmental Biology 2022Quote: ... and mRNA was captured and amplified with the Smart-seq2 v4 kit (Clontech). After AMPure XP beads purification ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was prepared using SMARTer procedure with SMARTer Ultra Low RNA kit (Clontech) and sequenced on Novaseq 6000 platform (Illumina).
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bioRxiv - Developmental Biology 2022Quote: ... Undifferentiated hESC HUES4 WT and PDXeG clone 170-324 were adapted and maintained in DEF-CS (Takara). When reaching approximately 80% confluence ...
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bioRxiv - Developmental Biology 2022Quote: ... Immunoprecipitated DNA and input control (pooled DNA that did not go through the IP step) were finally processed into sequencing libraries using PrepX DNA Library Kit (400075, Takara) and sequenced using the Illumina platform (NextSeq 500 ...
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bioRxiv - Developmental Biology 2022Quote: ... Extracted total RNA was reverse transcribed into cDNA using PrimeScript RT reagent Kit (TaKaRa RR037a) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... The titer of retrovirus was measure by Retro-X qRT-PCR Titration Kit (Clontech). For iPSC generation ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.6 μl of reaction mix (16.7 U/μl SMARTScribe TM Reverse Transcriptase (Takara Bio, 639538), 1.67 U/μl Recombinant RNase Inhibitor (Takara Bio ...
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bioRxiv - Developmental Biology 2022Quote: ... Lysis plates were created by dispensing 0.4 μl lysis buffer (0.5 U Recombinant RNase Inhibitor (Takara Bio, 2313B), 0.0625% Triton X-100 (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... SMARTerR Stranded Total RNA-seq kit v2-Pico InputMammalian (Takara Clontech) was used ...