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Citations for Takara Bio :
7401 - 7450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... GFP-tagged PDE5A isoforms plasmids [19] were co-transfected with pDsRed-mito (Clontech) into HEK293 cells using Lipofectamine 3000 (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... TB Green™ Premix Ex Taq™ II kit (Takara, RR820A) and CFX96TM-Real Time Systerm (Biorad ...
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bioRxiv - Cell Biology 2022Quote: ... All other constructs were generated from the above mentioned constructs using In-Fusion cloning (Takara) or site-directed mutagenesis (QuikChange II XL ...
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bioRxiv - Cell Biology 2022Quote: Yeast two-hybrid analysis was performed with the Matchmaker Gold Yeast Two-Hybrid System (Takara). Y2H Gold and Y187 yeast strains were transformed with bait (pGBKT7 plasmids and derivatives ...
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bioRxiv - Cell Biology 2022Quote: pGADT7-TMEM11 and pGBKT7-TMEM11 were generated by cloning the TMEM11 cassette from pGFP-TMEM11 and ligating it into NdeI/BamHI sites of pGADT7 and pGBKT7 plasmids (Takara), respectively ...
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bioRxiv - Cell Biology 2022Quote: pLVX-Puro GFP-TMEM11 was generated by PCR amplifying TMEM11 from human cDNA and cloning into the XhoI/BamHI sites of pAcGFP1-C1 (Takara), followed by subsequent cloning of the GFP-TMEM11 cassette into the Xho/BamHI sites of pLVX-Puro (Takara ...
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bioRxiv - Cell Biology 2022Quote: ... and the presence of the kinA-GFP fusion was confirmed by western blotting analysis with a polyclonal anti-GFP antibody from Clontech. In addition ...
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bioRxiv - Cell Biology 2022Quote: ... followed by subcloning into pLVX-puro (Clontech). All constructs were sequenced before use ...
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bioRxiv - Cell Biology 2022Quote: Yeast-two-hybrid analysis was performed with the MATCHMAKER GAL4 Two-Hybrid System 3 (Clontech) essentially as described (39) ...
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bioRxiv - Cancer Biology 2022Quote: ... Induction of DSBs in U2OS-DSB reporter cells line was induced by addition of Shield-1 (1 uM, TAKARA 632189) and 4-OHT (200 nM ...
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bioRxiv - Cancer Biology 2022Quote: ... NPP-Pparpgc1a-OE and NPP-Ppara-KO constructs were generated using InFusion Kit (Clontech, 638917) and T4 DNA Ligase (NEB ...
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bioRxiv - Cancer Biology 2022Quote: ... RNAseq libraries were made using the SMARTer ultra-low RNA kit V3 (Takara Bio USA, Mountain View, CA, USA). and analysed individually (i.e. ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA (1 μg) was reversed to cDNA with PrimeScript™ RT reagent Kit (Takara, RR047Q), which was performed according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and each reaction contained 1 μl of cDNA and the SYBR Green PCR mix (Takara, RR420A), to which gene-specific forward and reverse PCR primers were added ...
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bioRxiv - Cell Biology 2022Quote: ... and 72 hours after transfection and concentrated using the Lenti-X concentrator (Contech Takara, Cat# 631231) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: All expression plasmids are based on the lentiviral vector pLVX-EF1α-mCherry-N1 (Takara, Cat# 631986). Codon-optimized variants of GPR133 were previously published and described (Frenster et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Cell pellets was collected and isolated for total RNA with MiniBEST Universal RNA Extraction Kit (Takara, #9767). Total RNA (1 μg ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA for experiments was obtained using endotoxin-free plasmid purification kits (NucleoBond Xtra Midi EF, Takara, cat ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA (1μg) was reverse transcribed with PrimeScript RT Reagent Kit (Takara).
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bioRxiv - Cancer Biology 2022Quote: ... A cDNA library was produced using a SMARTer library preparation protocol (Clontech). Sequencing (100-bp paired-end reads ...
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bioRxiv - Cancer Biology 2022Quote: Four hundred nanogram total RNA was employed for cDNA synthesis using ProtoScriptII First Strand cDNA Synthesis kit (TaKaRa,#RR036A) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... The supernatant was filtered through a 0.45 µm filter and concentrated with Lenti-x concentrator (Takara Bio Inc., 631231) and 250 µl aliquots stored at −80°C.
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bioRxiv - Cancer Biology 2022Quote: ... Lentivirus was generated by transiently transfecting Lenti-X 293T cells (Takara Bio) with lentiviral packaging mix (Cellecta) ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein was purified by TALON affinity (Clontech), HiTrap-Q anion-exchange (Cytiva ...
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bioRxiv - Synthetic Biology 2022Quote: ... VH and VL segments were ordered as gene blocks from Integrated DNA Technologies and were cloned into linearized CMV/R backbones with 5X In-Fusion HD enzyme premix (Takara Bio).
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bioRxiv - Synthetic Biology 2022Quote: Plasmids were transformed into Stellar competent cells (Takara Bio), and transformed cells were grown at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... A second construct for total gene deletion was generated by cloning the 5’ (843 bp) and 3’ (292 bp) homology regions amplified by PCR using a Taq DNA polymerase with proofreading activity (Takara) and primers P9 + P12 and P13 + P14 into the pL0001 vector (BEI Resources ...
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bioRxiv - Molecular Biology 2022Quote: ... was amplified from genomic DNA and cloned into a pENTR/D plasmid by InFusion (Takara) to generate pENTR-gTPF2 ...
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bioRxiv - Molecular Biology 2022Quote: ... Anti-6xHis (631212, 1:10,000) from Clontech. Anti-Ubiquitin (646302 ...
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Activation of endoplasmic reticulum stress via clustering of the inner nuclear membrane protein SUN2bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from cells using the NucleoSpin RNA Kit (Takara Bio) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Primers were designed using the primer design tool from Takara Bio (https://www.takarabio.com/learning-centers/cloning/primer-design-and-other-tools) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cleared supernatant was incubated with nickel resin (His60 NiNTA, Clontech) for one hour at 10° C ...
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bioRxiv - Molecular Biology 2022Quote: ... and purified with NucleoSpin Gel and PCR Clean-up kit (Takara, Cat. No. 74061) for Sanger sequencing ...
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bioRxiv - Molecular Biology 2022Quote: ... and the RNA was concentrated and reverse transcribed with a 5’-RACE protocol that appends a new primer site to the cDNA of both cleaved and uncleaved RNA (SMARTScribe, Takara). The cDNA was PCR amplified with primers that add the adaptors for Illumina sequencing ...
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bioRxiv - Neuroscience 2022Quote: ... pRC2-mi342 and pHelper plasmids (TAKARA). Seventy-two hours after transfection using Polyethylenimine HCl Max ...
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bioRxiv - Molecular Biology 2022Quote: The 5’ end of RiMCO1 was verified by rapid amplification of cDNA ends (RACE) using the SMART RACE cDNA amplification kit (Clontech, Palo Alto, CA, USA), the RiMCO1-specific primer GiFETa.rR (Table S2 ...
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bioRxiv - Molecular Biology 2022Quote: ... pGADT7 and pGBKT7 plasmids (Clontech) were transformed ...
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bioRxiv - Molecular Biology 2022Quote: ... These fragments were combined to amplify the full genomic sequence that was cloned into a pENTR/D plasmid by InFusion (Takara) to generate pENTR-gMINU2 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... was used to transfect cells at 70% confluency with 20 ng of CRE-SEAP reporter plasmid (Clontech) and 20 ng of receptor or empty vector pcDNA-Zeo-tetO DNA per well ...
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bioRxiv - Neuroscience 2022Quote: ... washed with 200 µL water, and resuspended in PCR mix (22 µL water, 25 µL Terra PCR direct buffer, 1 µL Terra Polymerase (Takara), 1 µL 100 M Truseq PCR handle primer ...
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bioRxiv - Neuroscience 2022Quote: ... 0.5 μL SMARTScribe™ Reverse Transcriptase (Clontech), 2 μL 5x First Strand buffer ...
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bioRxiv - Neuroscience 2022Quote: ... and reverse transcription performed with RNA to cDNA EcoDry Premix (Takara) according to manufacturer’s instruction ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented with 2 µg/ml doxycycline (Clontech, 631311) for 3 days prior to electroporation and to induce Cas9 nickase expression ...
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bioRxiv - Neuroscience 2022Quote: ... media was collected and concentrated 1:10 in 1xPBS using Lenti-X concentrator (Takara Bio, #631231), aliquoted ...
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bioRxiv - Neuroscience 2022Quote: ... Ribo-depleted sequencing libraries were prepared with the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (TaKaRa Biotech) following manufacturer instructions and sequenced with Genewiz/Azenta on an Illumina NovaSeq S4 machine using a 2 ξ 150 bp pair-end read configuration to a minimum depth of 40 million reads per sample ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Complementary DNA were synthesized by reverse transcription using PrimeScriptTMRT Master Mix (TaKaRa, Dalian, China). qPCR was achieved on a Roche LC96 Real-Time PCR Detection System with 2 × T5 Fast qPCR Mix (Tsingke Biotechnology ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... SmartScribe RTase kit was acquired from Clontech (Mountain View, CA, USA). Phusion® High-Fidelity DNA Polymerase was from NEB (Beverly ...
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bioRxiv - Neuroscience 2022Quote: ... The titer was measured using a Lenti-X qRT-PCR titration kit (Clontech 631235).
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bioRxiv - Physiology 2022Quote: ... Kidney fibrosis was induced by one daily intraperitoneal injection of 0.2 μg/g body weight for 5 days of the chemical AP20187 (Takara Bio Inc. Kusatsu, Japan), in 8-10 weeks-old male transgenic mice ...
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bioRxiv - Neuroscience 2022Quote: ... 2.5 mM dNTP and 2 U/mL of recombinant RNase inhibitor (Clontech) then spun down and frozen at −80°C ...