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Citations for Takara Bio :
7351 - 7400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... phosphorylated by T4 polynucleotide kinase (TaKaRa) and inserted between SpeI and NotI restriction sites (pFastBac1-hCAP-D3-[3C]-StrepII ...
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bioRxiv - Cell Biology 2022Quote: ... phosphorylated by T4 polynucleotide kinase (TaKaRa), and inserted between BamHI and EcoRI restriction sites by using restriction enzymes and a DNA ligation kit (TaKaRa) ...
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bioRxiv - Cell Biology 2022Quote: ... and inserted between SpeI and NotI restriction sites (pFastBac1-hCAP-D3-[3C]-StrepII) by using restriction enzymes and a DNA ligation kit (TaKaRa). To create the hCAP-H2 construct with an N-terminal StrepII-tag (pFastBac-StrepII-[3C]-hCAP-H2) ...
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bioRxiv - Cell Biology 2022Quote: ... Fimbrin Fim1 was expressed in Escherichia coli and purified via His-tag affinity to Talon Metal Affinity Resin (Clontech, Mountain View, CA) (Skau & Kovar ...
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bioRxiv - Cell Biology 2022Quote: ... The extract was incubated for 1 h at 4 °C with Talon resin (Clontech, Mountain View, CA), loaded onto a column ...
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bioRxiv - Cell Biology 2022Quote: ... and concentrated 40-fold with Lenti-X Concentrator (Takara). The concentrated lentivirus was used immediately or stored at −80°C ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% Tet system approved FBS (Takara Bio USA), 1mM sodium pyruvate ...
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bioRxiv - Cancer Biology 2022Quote: ... using the SYBR green PCR master mix (Takara). Reactions were performed in duplicate or triplicate ...
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bioRxiv - Cell Biology 2022Quote: ... All lentiviral particles were produced in Lenti-X 293T cells using the Lenti-X HTS Packaging System (Takara, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... the respective fragments were excised with EcoRI and ligated into pCMV-Myc (Clontech Laboratories, Mountain View, CA). To generate the constructs coding for the double tagged Sun4 peptide sequences ...
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bioRxiv - Cell Biology 2022Quote: ... the resulting Myc-tagged versions were then cloned into the BamHI site of pEGFP-N3 (Clontech Laboratories). Finally ...
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bioRxiv - Cell Biology 2022Quote: ... RNA-seq libraries were generated by using SMART-Seq® Stranded Kit (TaKaRa bio), and sequenced by Novaseq 6000 ...
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bioRxiv - Cell Biology 2022Quote: ... and then the medium was switched to Ndiff227 (Takara Bio) supplemented with hbFGF (Fujifilm,10 ng/ml ...
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bioRxiv - Cell Biology 2022Quote: ... followed by subsequent cloning of the GFP-TMEM11 cassette into the Xho/BamHI sites of pLVX-Puro (Takara) by isothermal assembly ...
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bioRxiv - Cancer Biology 2022Quote: ... pooled and filtered through a 0.45 μm syringe filter and then concentrated using the Lenti-X Concentrator solution (Clontech/Takara, Saint-Germain-en-Laye, France). Intestinal WT organoids were expanded 3 days prior to infection in normal growth medium supplemented with 1 μg/ml R-Spondin ...
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bioRxiv - Cancer Biology 2022Quote: ... pooled and filtered through a 0.45 μm syringe filter and then concentrated using the Lenti-X Concentrator solution (Clontech/Takara, Saint-Germain-en-Laye, France). Intestinal WT organoids were expanded 3 days prior to infection in normal growth medium supplemented with 1 μg/ml R-Spondin ...
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bioRxiv - Cell Biology 2022Quote: ... followed by cDNA synthesis using SMARTer PCR cDNA synthesis Kit (Takara Bio).
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bioRxiv - Cell Biology 2022Quote: ... pyriformis were amplified using PrimeSTAR MAX DNA Polymerase (Takara Bio) from cDNA and cloned into the TagVag2 vector (Hrdy et al. ...
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bioRxiv - Cancer Biology 2022Quote: Genotyping was done using the Terra PCR Direct Polymerase Mix and kits (Takara Bio; Shiga, Japan). The primers used for genotyping are given in supplementary table 1 ...
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bioRxiv - Cancer Biology 2022Quote: ... Strbp and Upf1 (Table S1) were inserted between the BamHI and EcoRI sites of the knockdown vector pSirenRetroQ-puro (Clontech). The knockdown vectors for mouse Cebpb (Cebpb1 shRNA ...
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bioRxiv - Developmental Biology 2022Quote: ... coli (Takara Biosciences, Cat#636763) at an efficiency of 1.79×109 cfus/µg ...
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bioRxiv - Developmental Biology 2022Quote: ... The PrimeScriptTM RT reagent kit with gDNA eraser (Takara) was used to convert total RNA to cDNA ...
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bioRxiv - Developmental Biology 2022Quote: ... We performed qPCR using the TB Green Premix Ex Taq II (RR820; Takara) and CFX96 Real-Time System (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... We then performed LaTaq PCR (Takara, RR002A) to amplify the β-catenin transcripts ...
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bioRxiv - Cancer Biology 2022Quote: ... Forty ng cDNA was used as templates for RT-PCR using SYBR® Premix Ex Taq kit (TaKaRa, #RR820A) using LightCycler 96 (Roche ...
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bioRxiv - Developmental Biology 2022Quote: ... Single-end polyA+ RNA-Seq (mRNA-seq) libraries were prepared using the SMARTer (Takara) Low input RNA kit for Illumina sequencing ...
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bioRxiv - Developmental Biology 2022Quote: ... Single-cell cDNAs were prepared on Fluidigm C1 system by using SMARTer v4 Ultra Input Low RNA kit for the Fluidigm C1 System (Clontech). Only single-cell cDNAs confirmed by imaging data were performed quality check (QC ...
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bioRxiv - Developmental Biology 2022Quote: ... the maximuM amount (10.5 µl) was used for library preparation using the SMART-Seq HT kit (Takara, Cat. No. 634437) followed by Nextera XT DNA Library Preparation Kit (Illumina ...
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bioRxiv - Developmental Biology 2022Quote: ... rabbit anti-DsRed (632496, Clontech). EdU+ cells were detected using the Click-iT EdU Imaging kit (Molecular Probes ...
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bioRxiv - Cancer Biology 2022Quote: ... The ssDNA template was synthesized using GuideIt Long ssDNA kit (Takara #632666) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: sgRNA sequences were amplified from gDNA by PCR using Ex Taq DNA polymerase (TaKaRa). PCR mixes were prepared with 10 µL 10X reaction buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... the viral supernatant was concentrated 10 times with Lenti-X concentrator (Takara). Media were changed at 24 hours after infection and antibiotics (2 μg/ml puromycin ...
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bioRxiv - Cell Biology 2022Quote: ... 5um sections from paraffin blocks were used for immunofluorescence staining with the following primary antibodies: tdTomato (dsRed Mouse: Takara Biosystems 632392, Rabbit: Takara Biosystems 632496), GFP (Abcam 6673) ...
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bioRxiv - Cell Biology 2022Quote: The cDNA synthesis was done using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech) and the library was prepared using Nextera XT DNA Library Prep kit (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... 5um sections from paraffin blocks were used for immunofluorescence staining with the following primary antibodies: tdTomato (dsRed Mouse: Takara Biosystems 632392 ...
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bioRxiv - Cell Biology 2022Quote: ... The targeting vector was generated by In-Fusion Cloning (Takara Bio Inc, Shiga, Japan), and arms of homology were amplified from genomic DNA of the V6.5 ES cells to be targeted ...
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bioRxiv - Cell Biology 2022Quote: ... and pEGFP-C1 (Clontech) using EcoRI/KpnI restriction enzymes (NEB) ...
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bioRxiv - Cell Biology 2022Quote: ... Lentiviral titers were determined using the LentiX-p24 Rapid Titer ELISA kit (Takara). All cell culture reagents were obtained from ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... All Lck constructs were cloned in the expression vector pLVX-Tight-Puro (Clontech Laboratories, Inc.), between 5’ NotI and 3’ EcoRI restriction sites ...
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bioRxiv - Cell Biology 2022Quote: ... Human embryonic kidney epithelial Lenti-X293T (Clontech) cells were cultured in complete DMEM (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... inducible cell lines were generated using the Lenti-X Tet-On-Advanced Inducible Expression System (Clontech Laboratories, Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... JCaM.1-derived cell lines with tetracycline-inducible gene expression system were maintained in RPMI 1640 supplemented with 10 % tetracycline-negative FBS (Clontech). Cells were routinely tested and found negative for mycoplasma and were not STR profiled.
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bioRxiv - Cell Biology 2022Quote: ... the relevant AD and BD constructs were co-transformed into the auxotrophic yeast strain AH109 using the polyethylene glycol/lithium acetate method according to the manufacture’s manual (Clontech). Yeast cells haboring the relevant combinations of constructs were dotted on plates with double (-Leu-Trp) ...
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bioRxiv - Cell Biology 2022Quote: ... For SYBRGreen (Takara) based qPCR ...
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bioRxiv - Cell Biology 2022Quote: ... cells were unroofed after 15 min incubation with 500 nM AP21967 (500 μM stock in ethanol) (Takara, 635055) or 0.1% w/v ethanol (control ...
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bioRxiv - Cell Biology 2022Quote: ... the lentivirus were concentrated using Lenti-X concentrator (Takara Bio, Catalog #: 631231) and quantified with the qPCR lentivirus titration kit (Abmgood ...
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bioRxiv - Cell Biology 2022Quote: ... full length double-stranded cDNA was prepared from 1 ng of total RNA according to the manufacturer’s instructions for the SMART-Seq v4 Ultra Low Input RNA Kit® (Clontech). Quantity and quality of the amplified full length double-stranded cDNA were assessed using both Qubit (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... The cDNA was amplified via PCR and cloned into the appropriate vector backbones (empty pCS2+ or pCS2+ with C-terminal 3xeGFP) using InFusion Assembly (Takara). The GAP-dead mutant (RGA-3/4R80E ...
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bioRxiv - Cell Biology 2022Quote: ... 2U/μl RNase inhibitor (ClonTech, cat: 2313B), 2 mM dNTP’s (ThermoFisher Scientific ...
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KIF24 controls the clustering of supernumerary centrosomes in pancreatic ductal adenocarcinoma cellsbioRxiv - Cell Biology 2022Quote: ... human KIF24 fragments encoding residue 1-4383 was excised from pEGFP-C1-KIF24 (Kobayashi et al., 2011) and sub-cloned into pLVX-IRES-Puro (Clontech). KIF24/TS621-622AA (TS ...