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Citations for Takara Bio :
7251 - 7300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... eGFP-PolyQ31 was adapted from eGFP-PolyQ74 through the variability of Q-length PCR products amplified using CloneAmp HiFi PCR Premix (Takara). FM5-NPM1-mCh was generated as described in 27.
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bioRxiv - Biophysics 2022Quote: ... with insertion of N-terminal residues MATLEK a part of the N17 domain and C-terminal residues PQAQPLLPQPQPPPPPPPPPPGPAVAEEPLHRP which comprise the PR (proline-rich) domain containing the C38 domain using primers as a part of In-Fusion cloning protocol (Takara). eGFP-PolyQ31 was adapted from eGFP-PolyQ74 through the variability of Q-length PCR products amplified using CloneAmp HiFi PCR Premix (Takara) ...
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bioRxiv - Cancer Biology 2022Quote: ... These clones were further tested for transfectability using pmCherry-N1 control vectors (Takara Bio #632523). 200’000 cells of selected monocolonies of either cell line were seeded in 6-well plates and transfected on next day with CRISPR plasmids using Lipofectamine 2000 (Invitrogen by Life Technologies #11668019 ...
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bioRxiv - Biophysics 2022Quote: ... HEK-D (see below) and Lenti-X 293T (Takara) cells were cultured in growth medium consisting of Dulbecco’s modified Eagle’s medium (GIBCO) ...
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bioRxiv - Cancer Biology 2022Quote: ... MB CDS were ligated in XhoI and BamHI digested pmCherry-C1 (Takara Bio #632524) or in HindIII and BamHI digested pmCherry-N1 (Takara Bio #632523 ...
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bioRxiv - Bioengineering 2022Quote: 500 ng of total RNA extracted from cell culture were subjected to reverse transcription, using ISOGEN II (NIPPON GENE, Tokyo, Japan) and PrimeScript RT reagent Kit (TaKaRa, Kusatsu, Japan). The resulting cDNA was subjected to real-time PCR analysis using glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Plant Biology 2022Quote: ... protein solution was collected and subjected to purification using His60 Ni Superflow resin (TaKaRa, California, USA) to remove 6×His-SUMO tag from the protein preparations ...
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bioRxiv - Plant Biology 2022Quote: ... The concentrated supernatant was purified using His60 Ni Superflow resin (TaKaRa, California, USA) on a BioLogic LP system (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... The supernatant was collected and purified using His60 Ni Superflow resin (TaKaRa, California, USA) on a BioLogic LP system (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: ... the coding sequences were cloned into pColdI vector (Takara Bio Inc.).
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bioRxiv - Cell Biology 2022Quote: ... PCR reactions were carried out using Hot Start TaKaRa LA Taq kit (Takara Biotechnology, #RR042A) with the following cycling conditions ...
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bioRxiv - Cell Biology 2022Quote: RNA was extracted at each time point using the Nucleospin RNA XS kit from Clontech (cat. num. 740902.250). RNA quality was assessed using a NanoDrop and then Bioanalyzer was used to quantify RNA amount ...
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bioRxiv - Cancer Biology 2022Quote: ... Truncations of ENDOD1 and mutations of TP53 were obtained by fusing different PCR fragments using In-Fusion cloning kit (Clontech, 639650). Primers used in this study were listed in Supplementary Table S3.
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bioRxiv - Cancer Biology 2022Quote: ... using the In-Fusion cloning kit (Clontech, 639650). Point mutants for ENDOD1 and TP53 were converted from parental pLVX-Flag -IRES-ZsGreen1 plasmids using QuikChange Lightning Site-Directed Mutagenesis Kits (Stratagene ...
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bioRxiv - Cell Biology 2022Quote: ... Puromycin was from Takara Bio ...
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bioRxiv - Cell Biology 2022Quote: ... products were cut with KpnI/AgeI and cloned in frame into the same sites of pEGFP-N1 (Clontech). The Gαo-Nt8–31-GFP sequence was PCR-amplified from Gαo-Nt31-GFP ...
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bioRxiv - Cell Biology 2022Quote: ... and a pAb anti-GFP (632592, WB: 1/1000) was from Takara. All secondary Abs for immunofluorescence (IF ...
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bioRxiv - Cell Biology 2022Quote: ... AAV.Syne4AA was produced at Tel Aviv University using AAVpro Purification Kit Maxi (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-His (Clontech 631212), anti-H3K36me2 (Upstate 07-369) ...
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bioRxiv - Plant Biology 2022Quote: ... Bait and Prey constructs were prepared by restriction enzyme cloning with EcoRI and BamHI inserts into the pGBKT7 and pGADT7 plasmids (Clontech). Bait constructs were transformed into the haploid yeast strain Y2HGold (MATa trp1-901 leu2-3,112 ura3-52 hi3-200 gal4Δ gal80Δ LY2::GAL1UAS-Gal1TATA-Hi3 GAL2UAS-GAL2TATA-Ade2 URA3::MEL1UAS-Mel1TATA AUR1-C MEL1) ...
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bioRxiv - Plant Biology 2022Quote: ... Mating experiments were performed following Matchmaker Gold Yeast Two-Hybrid System (Clontech). Diploids were replica plated to media that selects for reporter gene activity of HI3 ...
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bioRxiv - Cell Biology 2022Quote: ... D/D solubilizer (635054) from Takara, VECTASHIELD Mounting Medium (H-1400 ...
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bioRxiv - Neuroscience 2022Quote: ... and amplified genomic DNA by PCR using the PrimeSTAR HS DNA Polymerase (TaKaRa) or KOD-plus Ver ...
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bioRxiv - Plant Biology 2022Quote: ... the constructs were prepared using In-Fusion HD cloning technology (Clontech, Mountain View, CA, USA). For DAIpro::DAI ...
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bioRxiv - Bioengineering 2022Quote: ... Lenti-X 293T cells were purchased from Takara and cultured according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... which was constructed from pCold I (Takara Bio Inc., Kusatsu, Japan) by replacing the sequences of the histidine tag and proteinase digestion site with that of pET-15b ...
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bioRxiv - Bioengineering 2022Quote: ... and Lenti-X™ 293T (632180, Takara Bio, CA, USA). These cells were maintained in 2D culture using the complete 2D growth media ...
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bioRxiv - Synthetic Biology 2022Quote: ... LP cassettes were cloned into PmeI-linearized pIDTsmart backbones between the left and right homology arms using In-Fusion cloning (Takara Bio USA). Using this approach ...
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bioRxiv - Synthetic Biology 2022Quote: ... and then cloned into corresponding entry vectors using In-Fusion cloning (Takara Bio, San Jose, CA, USA) or a one-step Gibson reaction with the in-house master mix ...
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bioRxiv - Cell Biology 2022Quote: ... Libraries were created by UCL Genomics Core Facility using the SMARTer Stranded Total RNAseq Kit (Clontech), cleaned using JetSeq (Bioline ...
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bioRxiv - Biochemistry 2022Quote: ... with (GGGGS)3 linker using In-Fusion HD Cloning Kit (Takara). Plasmids to express CAHS deletion mutants (CAHS3ΔCtail ...
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bioRxiv - Biochemistry 2022Quote: ... varieornatus [16] and inserted into pAcGFP1-N1 or pAcGFP1-C1 (Clontech) with (GGGGS)3 linker using In-Fusion HD Cloning Kit (Takara) ...
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bioRxiv - Biochemistry 2022Quote: ... and CAHS3-GFP was similarly inserted into a pCold-I vector (Takara). For expression in Drosophila cells ...
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bioRxiv - Biochemistry 2022Quote: ... CAHS3-GFP and AcGFP1 were expressed using a cold shock expression system (Takara) essentially as described previously [55] ...
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bioRxiv - Neuroscience 2022Quote: ... reverse-transcribed (TaKaRa), amplified ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmid expressing MeCP2 and GFP tagging was made by cloning the cDNA into pLVX-EF1α-IRES-ZsGreen1 vector (TaKaRa plasmid #631982). R168X mutation was introduced by site-directed mutagenesis ...
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bioRxiv - Biochemistry 2022Quote: ... Polyhistidine tagged IDE was isolated from the soluble lysate fraction by immobilized nickel affinity chromatography (NI-IDA resin, Takara Clontech Inc.) in accordance with manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: HIV-1 Gag (75) was ligated into pEGFP-N1 (Clontech, Takara Bio, Mountain View, CA) to generate the HIV-1 Gag-EGFP vector ...
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bioRxiv - Immunology 2022Quote: Lenti-X 293T cells (Takara Bio) were transfected with pHR SIN including cloned transgene and packaging vectors pMD2.G and pCMVdR8.91 using TransIT-Lenti Transfection Reagent (Mirus #MIR6603) ...
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bioRxiv - Microbiology 2022Quote: ... The two-hybrid system Matchmaker 3 from Clontech was used as per manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: HIV-1 Gag (75) was ligated into pEGFP-N1 (Clontech, Takara Bio, Mountain View, CA) to generate the HIV-1 Gag-EGFP vector ...
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bioRxiv - Developmental Biology 2022Quote: ... After extraction mRNA was reverse-transcribed into cDNA with PrimeScript RT Reagent Kit (Takara Bio), followed by quantitative PCR with Power SYBR Green PCR Master Mix (Applied Biosystems) ...
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bioRxiv - Biochemistry 2022Quote: ... Polyhistidine tagged IDE was isolated from the soluble lysate fraction by immobilized nickel affinity chromatography (NI-IDA resin, Takara Clontech Inc.) in accordance with manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Primary antibodies against GFP (Clontech, JL-8; 1:5000), G-6-PDH (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... and V2HeR1 was subcloned into a peGFP-P2A vector or pCaMKIIa-eGFP-P2A vector using an In-Fusion HD cloning kit (Takara Bio, Inc., Shiga, Japan) according to the manufacturer’s instructions ...
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MEK inhibitor resistance in lung cancer cells associated with addiction to sustained ERK suppressionbioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% tetracycline-free FBS (Clontech, 631101) and Pen/Strep (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... was amplified by PCR and subcloned into pBacPAK-His3-GST construct (provided by Dr. Svend Kjaer, The Francis Crick Institute) via In-Fusion cloning method (Takara Bio). StrepII2x-ATG5 ...
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bioRxiv - Neuroscience 2022Quote: ... in PBS] for 90min followed by an incubation with a rabbit anti-DsRed antibody (1:2000, in blocking solution; Takara Bio-Living Colors, RRID #632496) overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was generated from 1ng of input RNA using the SMART-Seq HT Kit (Takara 634455) at half reaction volume ...
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bioRxiv - Bioengineering 2022Quote: About 130 ml of clarified cell suspension was combined with 3 mL of TALON® resin (Takara Bio 635503) previously equilibrated in lysis buffer and rocked at 4°C for 1 h ...