Labshake search
Citations for Takara Bio :
7301 - 7350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: BM-MSC was generated by retroviral infection using pQCXIP (Clontech Laboratories, Inc., USA) with GFP gene sequence ...
-
bioRxiv - Cancer Biology 2022Quote: ... First-strand cDNA synthesis was performed with the Smart-seq v4 Ultra Low Input kit (Takara) using the 3’ Smart-seq CDS Primer IIA and Smart-seq V4 oligonucleotide ...
-
bioRxiv - Cancer Biology 2022Quote: ... 3’ multiplexed RNA-sequencing was performed with the Takara SMART-Seq v4 3’ DE Kit (Takara 635040) followed by Nextera XT (Illumina FC-131-1024 ...
-
bioRxiv - Cancer Biology 2022Quote: RNA-sequencing: FASTQ files were demultiplexed using a java script from Takara. FASTQ files were mapped and transcript counts were enumerated using STAR (genome version mm10 and transcript version genecode M13) ...
-
bioRxiv - Biophysics 2022Quote: ... and inserted into pLPCX (Takara-Clontech). Retroviruses were then generated as previously described [51] and transduced into MDCK WT cells ...
-
bioRxiv - Biophysics 2022Quote: ... and ten-fold concentrated using the Lenti-X-concentrator reagent (Takara, #631232). NIH-3T3 cells are seeded 24 hours prior to transduction at a density of 10 000 per cm2 ...
-
bioRxiv - Biophysics 2022Quote: ... and inserted into pLPCX (Takara-Clontech). Retroviruses were then generated as previously described [51] and transduced into MDCK WT cells ...
-
bioRxiv - Biophysics 2022Quote: ... This plasmid expresses the PR isoform-B under a tetracycline controllable promoter (TetOff system, Clontech). To perform the SPT experiments ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... The standard curves were drawn using serially diluted pT7Blue (Takara Bio, Japan) plasmid carrying a kanamycin resistance gene fragment ...
-
bioRxiv - Genomics 2022Quote: ... sgRNA constructs were amplified upon transformation of DH5α competent cells (Takara) grown at 37ºC ...
-
bioRxiv - Biophysics 2022Quote: ... The lentivirus is generated by transfection of Lenti-X 293T cells (Takara, #632180) with pBOB-EF1-FastFUCCI-Puro (Addgene ...
-
bioRxiv - Biophysics 2022Quote: ... A Puromycin resistance plasmid (pPUR, Clontech, Cat No. 631601) was used as a selection marker ...
-
bioRxiv - Biophysics 2022Quote: MCF7 Tet-off cells (Clontech, Cat No. 631154) were grown on Dulbecco Modified Eagle Medium (DMEM ...
-
bioRxiv - Biochemistry 2022Quote: ... Proteins were bound to TALON IMAC resin (Clontech) overnight at 4 °C ...
-
bioRxiv - Biochemistry 2022Quote: ... HeLa cells were transfected with the pLVX-TetOn-Advanced plasmid (Clontech) and selected with 500 μg/mL G418 for 14 days ...
-
bioRxiv - Biochemistry 2022Quote: Expression inserts for cell lines were generated by PCR amplification and ligated into the pLVX-tight-Puro vector (Clontech) using the NEB HiFi DNA Assembly Kit (New England Biolabs ...
-
bioRxiv - Cell Biology 2022Quote: ... Dimerizer ligand (AP201870) (Takara, 635058), Halt protease inhibitor (ThermoFisher ...
-
bioRxiv - Microbiology 2022Quote: The BLV pol gene was measured in the genomic DNA samples of blood and tissue samples of cattle using real-time PCR with a BLV Detection Kit (Takara Bio, Otsu, Japan) with a LightCycler 480 System II (Roche Diagnostics ...
-
bioRxiv - Microbiology 2022Quote: ... The coding sequence of TgAP3β was cloned into pGADT7 (Clontech Laboratories, Mountain View, CA) and co-transformed into competent AH109 yeast cells with a plasmid encoding E ...
-
bioRxiv - Microbiology 2022Quote: ... total RNA was isolated from cells using Trizol RNA isolation kit (Takara, Shiga, Japan) and MDR1 and GAPDH transcripts were detected using a TransScript Green One-Step qRT-PCR SuperMix (TransGen Biotech ...
-
bioRxiv - Microbiology 2022Quote: Doxycycline-inducible stable cells overexpressing Trim69 were generated using the pRetroX-Tight system (Clontech), a murine leukemia virus (MLV ...
-
bioRxiv - Genomics 2022Quote: ... 100 ng of RNA was reverse transcribed into cDNA using PrimerScriptTM RT Master Mix (TaKaRa, #RR036A). Next ...
-
bioRxiv - Genomics 2022Quote: ... CH17-203N23 and CH17-449P15 BACs were extracted by using a NucleoBond Xtra BAC kit (Takara, 740436.25). ∼1 μg of BAC DNA was digested with 30 nM of sgRNAs (IDT) ...
-
bioRxiv - Genomics 2022Quote: ... 0.4 μl Prime STAR polymerase (Takara, R050B), filled up to 20 μl with water in a 2-step PCR reaction with 28 cycles (denaturation ...
-
bioRxiv - Genomics 2022Quote: ... Purification Kit Maxi (Takara, 6676) and quantified by the AAVpro Titration Kit (Takara ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Positive controls were treated with 50 μL DNase solution (TaKaRa) for 20 min ...
-
bioRxiv - Immunology 2022Quote: ... was amplified using a high-proof reading polymerase (CloneAmp HiFi, Takara) with primers containing EcoRI and NotI restriction sites and subcloned into pCR blunt II TOPO vector for subsequent restriction and ligation into the pcDNA3 expression vector ...
-
bioRxiv - Immunology 2022Quote: ... lentiviruses in the culture supernatants were concentrated by using lenti-X concentrator (Takara bio, 631231). iBMDMs were seeded into 6-well plates overnight at a density of 1×105 per well ...
-
bioRxiv - Cell Biology 2022Quote: ... The yeast was transformed with the indicated plasmids using the Matchmaker™ Yeast Transformation System 2 (Clontech, Cat#: 630439). Two plasmids containing simian virus (SV ...
-
bioRxiv - Cell Biology 2022Quote: ... coli DH5⊡ competent cells and isolated using a midi-prep kit (Takara Bio, 740420.10), and validated by gel electrophoresis.
-
bioRxiv - Cell Biology 2022Quote: Plasmid DNA (pEGFP-N1, 5kb (Clontech, 6085-1) encoding a cytomegalovirus (CMV ...
-
bioRxiv - Cell Biology 2022Quote: ... OCN (1:200, Takara Bio Inc, Shiga, Japan) overnight at 4°C followed by corresponding fluorescence-linked secondary antibodies (Jackson ImmunoResearch Laboratories ...
-
bioRxiv - Cell Biology 2022Quote: Full length wild type human RORβ (NM_006914.3) was subcloned into the pLPCX retroviral vector (Clontech) using XhoI and NotI restriction enzymes (NEB ...
-
bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used: 1:2000 mouse anti-GFP (RRID:AB_2313808, 632381, Clontech); 1:2000 mouse anti-3V5 (RRID:AB_2556564 ...
-
bioRxiv - Cell Biology 2022Quote: ... and 5 units of SMART Moloney murine leukemia virus reverse transcriptase (Takara Bio, Mountain View, CA, USA). The RT reaction was carried out at 42°C for 2 hrs ...
-
bioRxiv - Cell Biology 2022Quote: ... 1.0~5 μg of total RNA was reverse-transcribed in a 20-μl reaction containing 1x RT buffer (Clontech, Mountain View, CA, USA), 0.5 mM dNTPs ...
-
bioRxiv - Cancer Biology 2022Quote: ... cDNAs were diluted 1/10 and PCR reactions were conducted using SYBR qPCR Premix Ex Taq II Tli RNase H+ (TAKRR820W, Takara). Primers can be found in Supplementary Table 4 ...
-
bioRxiv - Cancer Biology 2022Quote: ... was inserted using the In-fusion HD cloning kit (Cat. No. 639648, Takara-bio) into the SpeI restriction site in the pPB-LR5 ...
-
bioRxiv - Cell Biology 2022Quote: ... into a pRetroX-TetOne-Puro (Takara) backbone using the EcoRI and BamHI restriction sites ...
-
bioRxiv - Cell Biology 2022Quote: ... followed by SDS–PAGE and immunoblotting with goat anti-GST (1:3000, Cytiva, 27-4577-01) and mouse anti-6xHis (1:3000, Takara Bio, 631212) antibodies ...
-
bioRxiv - Cancer Biology 2022Quote: ... and pEco (Takara, #PT3749-5). Cells transduced with the retrovirus were sorted for EGFP positive by flow cytometry ...
-
bioRxiv - Cancer Biology 2022Quote: ... and gel extracted using the NucleoSpin Gel and PCR Clean-up kit (Takara Bio USA Inc ...
-
bioRxiv - Cell Biology 2022Quote: Lenti-X HEK293T cells (Takara, Cat# 632180) were cultured according to the manufacturer’s protocols in DMEM (Gibco ...
-
bioRxiv - Cancer Biology 2022Quote: ... the products were amplified with PrimSTAR® Max DNA Polymerase (TaKaRa bio Inc.). The vector arms and PCR products were combined by incubating them for 1 hour at 50°C in the Gibson Assembly reaction buffer (5% PEG-8000 ...
-
bioRxiv - Cell Biology 2022Quote: ... The transformants were screened by microscopically observing the GFP signals and further confirmed by a western blotting analysis with a polyclonal anti-GFP antibody from Clontech. In addition ...
-
bioRxiv - Developmental Biology 2022Quote: ... were co-transfected into 293T cells (Clontech, 632180) with PEI reagent (Polysciences ...
-
bioRxiv - Developmental Biology 2022Quote: ... Quantitative PCR was performed using a TB Green Premix DimerEraser Kit (RR091A; Takara). The primer sequences are listed in Supplementary Table S1.
-
bioRxiv - Cell Biology 2022Quote: ... re-primed cells were cultured with Ndiff227 (Takara Bio) supplemented with hbFGF (Fujifilm,10 ng/ml) ...
-
bioRxiv - Cell Biology 2022Quote: ... with or without 1.59 nM (50 μg/μL) bacteriophage λ DNA (48502 bp; TaKaRa). The ratio between condensin II and λ DNA was 1 condensin II ...
-
bioRxiv - Cell Biology 2022Quote: ... and inserted between BamHI and EcoRI restriction sites by using restriction enzymes and a DNA ligation kit (TaKaRa). Constructs of hCAP-H2 harboring charge-reversal mutations in the BC1 and BC2 regions (H2-BC1D ...