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Citations for Takara Bio :
7201 - 7250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... The immunoprecipitated (RGA-GFP and RGAm2-GFP) and co-immunoprecipitated (IDD2-RFP) proteins were detected by western-blot with anti-GFP (JL8; Clontech) and anti- RFP (6G6 ...
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bioRxiv - Plant Biology 2022Quote: ... milk 5%) and incubated with a 2000-fold dilution of anti-GFP (JL8; Clontech), anti-RGA (Agrisera) ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were hybridized in ExpressHyb Hybridization Buffer (ClonTech) to random-primed 32P-labeled DNA probes (RadPrime DNA labelling system ...
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bioRxiv - Immunology 2022Quote: ... non-tissue culture treated 24-well plates were coated with RetroNectin (TaKaRa) overnight at 30 mg/mL ...
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bioRxiv - Genomics 2022Quote: ... and the ends were blunted using Mighty Cloning Kit (Cat# 6026, Takara Bio). DNA fragments of 4–5 kb were fractionated by agarose gel electrophoresis ...
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bioRxiv - Genomics 2022Quote: ... and T4 DNA polymerase (Cat# 2040, Takara Bio), ligated into pBluescript II KS(+) ...
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bioRxiv - Genomics 2022Quote: ... DNA samples that were shortened to the appropriate size were blunted with S1 nuclease (Cat# 2140, Takara Bio) and T4 DNA polymerase (Cat# 2040 ...
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bioRxiv - Immunology 2022Quote: Total RNA of the cells was extracted using Trizol and then treated with DNase I (Takara, Shiga, Japan) to deplete the genomic DNA according the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... with HisTALON™ Buffer Set (Clontech Laboratories, 635651) following manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... We used LA Taq with GC buffers (Cat# RR02AG, Takara Bio) or Multiplex PCR Plus Kit with Q-solution (Cat# 206152 ...
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bioRxiv - Genomics 2022Quote: ... and treated with DNase I (Cat# 2270A, Takara Bio). cDNA was synthesized using SuperScript III First-Strand Synthesis SuperMix (Cat# 18080400 ...
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bioRxiv - Genomics 2022Quote: ... ligated with pUC118/Hinc II BAP (Cat# 3322, Takara Bio), and used to transform E ...
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bioRxiv - Genomics 2022Quote: ... The insert fragment was extracted and treated with Exonuclease III (Cat# 2170, Takara Bio) typically at 23 °C for 3 ...
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bioRxiv - Immunology 2022Quote: ... p24Gag concentrations were quantitated using the Lenti-X p24Gag Rapid Titer Kit (Clontech). For infection ...
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bioRxiv - Immunology 2022Quote: ... Quantitative real-time PCR was performed using SYBR Premix EX Taq (Tli RNaseH Plus) (Takara) in a QuantStudio3 thermal cycler (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... Adding poly (A) tails to RNA (Takara) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... RNA was treated with gDNA Eraser (Takara) to remove genomic DNA first before1ug of total RNA was reverse transcribed for 15 or 20 min either with random hexamers or with strand-specific primers of FCREIA (Table S1) ...
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bioRxiv - Immunology 2022Quote: 5’ and 3’ RACE was performed with SMARTer RACE 5’/3’ Kit (Takara) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The purification was performed using TALON® Metal Affinity Resin from (Clontech Laboratories, Inc, 635501) with HisTALON™ Buffer Set (Clontech Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... was performed using one-step Prime script III RT-qPCR mix (RR600A, Takara, Kyoto, Japan). The viral RNA of nucleocapsid protein was detected by a 2019-nCoV-N1 probe (10006770 ...
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bioRxiv - Microbiology 2022Quote: ... and plasmid isolation and gel extraction kits were obtained from Takara Biotech (USA) ...
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bioRxiv - Microbiology 2022Quote: ... we used 1 μL of Extaq enzyme from Takara on genomic DNA (20 μg ...
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bioRxiv - Molecular Biology 2022Quote: ... Real-time RT-PCR analysis was performed using the Thermal Cycler Dice Real-Time System II (Takara Bio Inc., Shiga, Japan) to detect the mRNA expression of GPX4 ...
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bioRxiv - Molecular Biology 2022Quote: ... And realtime PCR amplification of the cDNAs were performed with SYBR® Premix Ex Taq™ (TAKARA) kit in ABI 7500 realtime-PCR system ...
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bioRxiv - Molecular Biology 2022Quote: ... LentiX293T (Takara Bio, Shiga, Japan) cells were cultured in DMEM supplemented with 10% FCS and 1 mM sodium pyruvate ...
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bioRxiv - Microbiology 2022Quote: ... LASVpp-BlaM virus was concentrated 10 times with Lenti-X concentrator (Clontech Laboratories, Mountain View, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... A549 and DF-1 cells stably expressing wild-type and the Ala384-deleted LAMP1 mutant (LAMP1-WT and LAMP1-d384) were generated by transduction with pQCXIP retroviral vectors (Clontech, Mountain View, CA) encoding LAMP1-WT and LAMP1-d384 ...
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bioRxiv - Neuroscience 2022Quote: ... RNA with RIN > 7 was prepared for sequencing using the RNAseq Ovation V2 kit (Nugen) or Smart-seq 4 low-abundance RNA kit (Takara) and sequenced on the Illumina HiSeq 4000 or Novaseq 2 platform in collaboration with Oxford Genomics Centre.
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bioRxiv - Molecular Biology 2022Quote: ... Ribo-depleted sequencing libraries were prepared with the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (TaKaRa Biotech) following manufacturer instructions and sequenced with Genewiz/Azenta on an Illumina NovaSeq S4 machine using a 2 x 150 bp pair-end read configuration to a minimum depth of 30 million reads per sample ...
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bioRxiv - Biophysics 2022Quote: ... 5’ and 3’-RACE-Ready cDNA templates were synthesized using a SMARTer® RACE 5’/3’ Kit (Takara Bio, USA) and subsequently used to amplify 5’ and 3’ end sequences of P ...
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bioRxiv - Biophysics 2022Quote: ... The PCR reaction was done with CloneAmp Hifi premix from Takara.
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bioRxiv - Biophysics 2022Quote: ... 3’-RACE-Ready cDNA template was synthesized using a SMARTer® RACE 5’/3’ Kit (Takara Bio, USA) and subsequently used to amplify 3’ end sequences of R ...
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bioRxiv - Biophysics 2022Quote: ... TALON cobalt resin (Takara) was washed with water then equilibrated with 10 column volumes of running buffer ...
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bioRxiv - Biophysics 2022Quote: ... E-cadherin (24E10-Cell Signaling Technology; DECMA1-Sigma Aldrich, ECCD2, Takara Bio) (1:100)and paxillin (Abcam ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... The cloned inserts were released by PstI and KpnI digestion and cloned into the eukaryotic expression vector pEGFP-C1 (Clontech) using the above procedure but with kanamycin selection ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Fifteen to thirty heads were divided into three and used for total RNA extraction with RNAiso Plus (TAKARA) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2022Quote: ... qRT-PCR was performed with SYBR Premix Ex TaqII (RR820A, Takara Bio Inc.) in an ABI 7300 thermal cycler (Applied Biosystems ...
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bioRxiv - Biochemistry 2022Quote: ... cDNA was synthesized with a Prime Script RT Reagent Kit (RR047A, Takara Bio Inc., Shiga, Japan). qRT-PCR was performed with SYBR Premix Ex TaqII (RR820A ...
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bioRxiv - Biochemistry 2022Quote: ... DNA was amplified by qRT-PCR with SYBR Premix Ex Taq II (RR820A, Takara) in ABI 7300 thermal cycler (Applied Biosystems ...
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bioRxiv - Biochemistry 2022Quote: ... Various KNL2 mutant DNAs were cloned into a pEGFP-C3 vector (Clontech), which were transfected into KNL2 knockout cells with Gene Pulser II electroporator (Bio-Rad) ...
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bioRxiv - Biochemistry 2022Quote: ... All PCRs were performed using PrimeSTAR Max polymerase (Takara Bio) per manufacturer’s instruction ...
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bioRxiv - Biochemistry 2022Quote: ... Cleared lysate was incubated with 400 μl of Talon Resin (TaKaRa) for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Transfection was carried out using Xfect transfection reagent following manufacturer’s instruction (Takara Bio 631318). After transfection ...
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bioRxiv - Biochemistry 2022Quote: ... HEK-293T cells were transfected with 1 µg of TRPC6 channel plasmids (WT, T221A and 112Q) using Xfect transfection reagent according to manufacturer’s protocol (Takara Bio 631318). Cells expressing wild type and mutant channels were identified by YFP fluorescence ...
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bioRxiv - Bioengineering 2022Quote: ... the gel-purified RNA was reverse transcribed into cDNA using a PrimeScript RT Reagent Kit with random primers (TAKARA, RR037B), followed by PCR with primers that can amplify transcripts across the splice junction ...
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bioRxiv - Bioengineering 2022Quote: ... Purified insert DNA was cloned into the linearized modRNA plasmid (5MCS3)(K et al., 2018) using the In-Fusion Cloning Kit (Takara Bio). The DNA template for modRNA synthesis was PCR amplified from the successfully cloned 5MCS3 plasmid followed by PCR purification using DNA Clean & Concentrator-5 (Zymo Research) ...
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bioRxiv - Bioengineering 2022Quote: ... The resin was then packed in a TALON 2 mL Disposable Gravity Column (Takara Bio), and the column was washed three times with 3 mL of washing buffer (50 mM sodium phosphate ...
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bioRxiv - Bioengineering 2022Quote: ... The supernatant was mixed with equilibrated TALON Metal Affinity Resin (200 μL of slurry, Takara Bio) for 1 h at 4 °C ...
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bioRxiv - Bioengineering 2022Quote: The cells were confirmed to be mycoplasma-negative every few months using a CycleavePCR Mycoplasma Detection Kit (CY232, Takara Bio).
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bioRxiv - Biophysics 2022Quote: ... double and triple mutants of SETD3 were created by site-directed mutagenesis using the In-Fusion cloning protocol (Takara) following manufacturer’s recommendations ...