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Citations for Takara Bio :
7151 - 7200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Reverse transcription was performed using PrimeScriptTM RT reagent kit (Takara), qPCR was performed using the SYBR Green Premix Ex TaqTM (Takara ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA was isolated from cells using the RNA extraction reagent (Takara). Reverse transcription was performed using PrimeScriptTM RT reagent kit (Takara) ...
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bioRxiv - Cancer Biology 2022Quote: ... qPCR was performed using the SYBR Green Premix Ex TaqTM (Takara) and specific primers ...
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bioRxiv - Cancer Biology 2022Quote: ... from cells and reversed transcribed into cDNA using PrimeScript RT Master Mix (TaKaRa, Japan). qPCR was performed to detect mRNA expression of genes by TB Green PCR Kit (TaKaRa ...
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bioRxiv - Cancer Biology 2022Quote: ... qPCR was performed to detect mRNA expression of genes by TB Green PCR Kit (TaKaRa) and primers are shown in table S2.
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bioRxiv - Biochemistry 2022Quote: ... supernatants were applied to pre-equilibrated cobalt-based affinity resins (Talon, Clontech) and allowed to bind at 4 °C for 1 h under gentle rotation ...
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bioRxiv - Cancer Biology 2022Quote: Using the SMARTer Thruplex DNA-seq kit (Takara), 50ng of sheared DNA were prepared for sequencing following the recommended instructions with samples undergoing 5 PCR cycles for unique sample indexing and library amplification ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA was extracted using the NucleoSpin® RNA XS Kit (Takara Bio, 740902.50). RNA quality and abundance were analyzed using a Bioanalyzer 2100 (Agilent RNA Pico Assay) ...
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bioRxiv - Cancer Biology 2022Quote: ... SMARTer Apollo NGS library prep system (Takara, Mountain View, CA) was used for automated poly(A ...
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bioRxiv - Cancer Biology 2022Quote: ... Reverse transcription was performed with 1 μg RNA using PrimeScript RT reagent Kit (Takara). The cDNA was amplified with gene-specific primers (Supplemental Table 1 ...
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bioRxiv - Cancer Biology 2022Quote: ... The cDNA was amplified with gene-specific primers (Supplemental Table 1) and SYBR Premix Ex Taq II kit (TaKaRa). Data were analyzed using a 2−ΔΔCt method.
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bioRxiv - Cancer Biology 2022Quote: Retroviral plasmids were constructed in pMSCV (Clontech, Palo Alto, CA) vectors ...
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bioRxiv - Cancer Biology 2022Quote: ... Lentivirus suspension was concentrated using Lenti-X concentrator per manufacturer standardized protocol (Takara Bio). Subsequently ...
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bioRxiv - Cancer Biology 2022Quote: Before undergoing ThruPLEX Tag-seq library preparation (Takara), samples were concentrated to 10μl using a vacuum concentrator (SpeedVac) ...
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bioRxiv - Microbiology 2022Quote: ... spleen and intestine (infected C57BL/6) by using TRIzol (Takara) reagent according to manufacturers’ protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Incubation with primary antibodies was performed in blocking buffer containing rabbit anti-DsRed (1:1000, 632496; Clonetech-Takara Bio, Japan) antibody at 4°C for 1–2 days ...
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bioRxiv - Neuroscience 2022Quote: ... vector encoding AAV9-CaMKII::G-CaMP7 (Titer: 9.4 × 1012 vg/mL) after 40- fold dilution with phosphate buffered saline (PBS) (T900; Takara Bio, Inc., Japan) was used41 ...
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bioRxiv - Microbiology 2022Quote: ... cells were transduced in 24-well plates precoated with recombinant fibronectin (FN CH-296, Retronectin Takara, Clontech, Mountain View, CA) with retroviral supernatants and expanded in complete medium (45% RPMI-1640 and 45% Click’s medium ...
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bioRxiv - Microbiology 2022Quote: ... PCR was performed using PrimeSTAR Max DNA Polymerase (Takara) with the cDNA as template and virus-specific primers 5’-CAC TCT TTC CCT ACA CGA CGC TCT TCC GAT CT NNN GGT CAC TAG TTG CCT CAT CCG G-3’ and 5’-GAC TGG AGT TCA GAC GTG TGC TCT TCC GAT CTG GTG CAT CTG ATC TCA TTA TTG-3’ ...
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bioRxiv - Microbiology 2022Quote: ... the sample was size fractionated using CHROMA SPIN 1000 columns to select for DNA sizes greater than 1 kb (Clontech, Mountain View, CA). After fractionation ...
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bioRxiv - Microbiology 2022Quote: ... Virus production was quantified by determining the amount of the Gag p24 protein using an enzyme-linked immunosorbent (ELISA) assay (Innogenetics or TaKaRa). For production of HIV-1 Env-pseudotyped viruses in Jurkat cells ...
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bioRxiv - Microbiology 2022Quote: ... 16S rRNA gene sequences were amplified by polymerase chain reaction (PCR) using LA Taq polymerase (TaKaRa-Bio, Inc., Kusatsu, Japan) for Illumina MiSeq paired-end sequencing ...
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bioRxiv - Microbiology 2022Quote: ... 2X ProteoGuard EDTA-free protease inhibitor cocktail (catalog no. 635673, Takara Bio USA, Inc., San Jose, CA, USA), and DNase and RNase-free RPI molecular grade water (catalog no ...
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bioRxiv - Plant Biology 2022Quote: ... and were tandemly combined and were inserted into AscI site in pZH_OsU6sgRNA _SpCas9 (Endo et al., 2019) by In-Fusion reaction (Takara), respectively ...
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bioRxiv - Plant Biology 2022Quote: ... followed by DNase I (Takara) treatment ...
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bioRxiv - Plant Biology 2022Quote: ... or anti-mCherry (632543, Takara) antibodies at dilutions of 1:3000 and 1:2000 ...
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bioRxiv - Molecular Biology 2022Quote: ... Random Primer Mix (Takara) and 200 U SMARTScribe Reverse Transcriptase (Takara) ...
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bioRxiv - Molecular Biology 2022Quote: GroES and GroEL were obtained from Takara Biotechnology (Dalian ...
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bioRxiv - Molecular Biology 2022Quote: ... pGADT7-AD (Takara Bio), pFN21A HaloTag CMV Flexi (Promega) ...
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bioRxiv - Molecular Biology 2022Quote: ... pGBKT DNA-BD and pGADT7-AD vectors (Takara Bio) containing cDNAs encoding regions of MYPN ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was extracted using TRIzol (Takara, China), and RT–PCR was performed ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.8 μg of pEGFP-N1 vector (Takara Bio USA, Mountain View, CA), which carries EGFP under the promoter of human Cytomegalovirus (CMV) ...
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bioRxiv - Neuroscience 2022Quote: ... For first-strand cDNA synthesis the Takara PrimeScript RT-PCR Kit (Takara Bio, Shiga, Japan) was used with 50 μM Oligo (dT ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-qPCR for mRNA detection was performed with the SYBR Premix Ex Taq II (Takara Biomedical Technology, China) using Bio-Rad iQ5 detection instrument (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... by using PrimeScript™ RT reagent (Perfect Real Time) Kit (TaKaRa, Japan). All PCR amplifications were performed in triplicate ...
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bioRxiv - Plant Biology 2022Quote: ... These plasmids were transformed into Saccharomyces cerevisiae strain AH109 (Clontech). At least 3 independent yeast transformants were checked for each pairwise interaction according to (Cuellar et al. ...
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bioRxiv - Plant Biology 2022Quote: ... PtoATPE and PtoPSBB) were co-transformed into the Y1HGold yeast strain using the Matchmaker One-Hybrid Library Construction and Screening Kit (Clontech), respectively ...
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bioRxiv - Microbiology 2022Quote: ... One μg of total RNA was used as input for SMARTer Stranded Total RNA Sample Prep Kit-HI Mammalian (Clontech). Sequencing was performed on the NextSeq500 instrument (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... CoV-229E sequences were obtained as gBlocks from IDT and subcloned into these plasmids using InFusion cloning (Takara).
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bioRxiv - Neuroscience 2022Quote: ... and MEFs were reverse-transcribed using a PrimeScript II first-strand cDNA Synthesis Kit (Takara Bio) with a random hexamer primer ...
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bioRxiv - Neuroscience 2022Quote: ... 3′RACE was performed using SMARTer RACE cDNA Amplification Kit (Takara Bio) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... We then assembled these fragments by CPER using PrimeSTAR GXL DNA polymerase (Takara Bio, Cat# R050A). The assembled cDNA was transfected into BHK-hACE2 cells using Lipofectamine LTX Reagent with PLUS™ Reagent (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... using the TAKARA Ligation kit ver.2.1 (TAKARA Bio, Cat# 6022).
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bioRxiv - Developmental Biology 2022Quote: ... Quantitative (q) RT-PCR was performed using SYBR Premix ExTaq 11 (Takara Bio) and detected using the Bio-Rad CFX96 Connect system ...
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bioRxiv - Developmental Biology 2022Quote: ... RNase inhibitor (Takara, cat. no. 2313A) and nuclease-free water (Ambion ...
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bioRxiv - Biochemistry 2022Quote: ... DNA ligation of the linearised pOPINTTGneo vector and the human UGGT1insert was achieved by In-fusion™ligation-independent cloning (Takara Ltd.)
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bioRxiv - Genetics 2022Quote: ... the pEGFP-N1 vector was used (Clontech, PT3027-5; Mountain View, CA). Mutagenesis was done using the QuikChange II XL kit (Stratagene ...
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bioRxiv - Microbiology 2022Quote: ... cerevisiae strain AH109 (Matchmaker 3 system, Clontech) was used ...
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bioRxiv - Plant Biology 2022Quote: ... GID1b and GID1c cDNAs inserted into pDONR221 were fused to the activation domain GAL4 (AD) after recombination into pGADT7 (Clontech).
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bioRxiv - Plant Biology 2022Quote: ... the N-terminal part is subject to self-activation in yeast64) inserted into pDONR221 were recombined into pGBKT7 (Clontech) to obtain BD- RGA ...