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Citations for Takara Bio :
7101 - 7150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... cDNA was prepared from 2 µg of each RNA sample using the PrimeScript II 1st strand cDNA synthesis kit (TaKaRa, Shiga, Japan) with 40 units of RNasin Plus RNase inhibitor (Promega ...
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bioRxiv - Immunology 2022Quote: ... added with RT-PCR master mix and IgG VH/VL primers per well and performed with RT-PCR following the one step RT-PCR kit protocol (Takara, RR057A). Then ...
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bioRxiv - Cell Biology 2022Quote: ... A pCMV backbone (Clontech) was used for the expression of full-length fluorescent protein (msfGFP/mApple ...
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bioRxiv - Microbiology 2022Quote: ... Western blots using Anti-His primary antibody (Clontech) (1:4000 dilution ...
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bioRxiv - Microbiology 2022Quote: ... and XbaI (Takara Bio) restriction sites ...
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bioRxiv - Cancer Biology 2022Quote: ... in which pRetroX-TetOne puro empty vector or pRetroX-TetOne puro-ALKBH5 were each cotransfected with the envelope vector VSV-G using Xfect transfection reagent (Clontech). BEAS2B ...
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bioRxiv - Cancer Biology 2022Quote: ... Retroviral supernatants were produced using the GP2-293 packaging cell line (Clontech), in which pRetroX-TetOne puro empty vector or pRetroX-TetOne puro-ALKBH5 were each cotransfected with the envelope vector VSV-G using Xfect transfection reagent (Clontech) ...
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bioRxiv - Cancer Biology 2022Quote: The retroviral plasmid pRetroX-TetOne puro (634307, Clontech, Mountain View, CA, USA) was amplified using NEB Stable competent Escherichia coli (high efficiency ...
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bioRxiv - Genetics 2022Quote: ... Lentiviral shRNA particles were prepared with Lenti-X 293T cells (Takara) and MISSION pLKO.1 plasmids (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... Chimeras were generated using In-Fusion Cloning (Takara Bio) and were confirmed by Sanger sequencing (Genewiz).
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bioRxiv - Microbiology 2022Quote: ... the FLAG-APEX2-GARG-1060 insert was cloned into the retroviral vector plasmid pLNCX2 (Takara Bio). Cloning details are available upon request ...
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bioRxiv - Microbiology 2022Quote: ... using the In-FusionHD Cloning Kit (Takara). Mutations in the coiled-coil domain were introduced by PCR with the primers listed in Table S1 ...
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bioRxiv - Microbiology 2022Quote: ... or IgL constant regions using In-Fusion cloning (Clontech, cat#638911) as previously described62.
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bioRxiv - Immunology 2022Quote: ... and then the expression construction of CMV-tdTomato was amplified from pCMV-tdTomato vector (Clontech, 632534) to inserted downstream of HA-R by GeneArt Gibson Assembly (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... Lenti-X HEK293T cells (Takara Bio, 632180) were cultured in DMEM with GlutaMAX (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... PCR products were purified using NucleoSpin® Gel and PCR Clean-Up Kit (Clontech). IVT was performed to generate dsRNA using the T7 High Yield RNA Synthesis Kit (NEB) ...
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bioRxiv - Immunology 2022Quote: ... 1 mL of retroviral supernatant was added to a non-tissue culture-treated 24-well plate pre-coated with Retronectin (FN CH-296; Takara Shuzo, Otsu, Japan) and centrifuged for 90 min at 2000×g ...
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bioRxiv - Immunology 2022Quote: ... the PCR product was assembled into an EcoRI + XhoI digested pMSCV-neo (Takara Clontech) vector by using Gibson Assembly Master Mix (NEB ...
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bioRxiv - Immunology 2022Quote: ... Ligated plasmids were then transformed into Stellar competent cells (Takara Clontech, 636763) and single clones were picked on the next day ...
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bioRxiv - Genetics 2022Quote: ... StellarTM cells (Takara) were transformed with the resulting plasmids ...
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bioRxiv - Immunology 2022Quote: 5 × 106 GP2-293 packaging cells (Clontech) were plated in a 10 cm dish containing D10 medium (DMEM supplemented with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... Retroviral supernatants were centrifuged onto RetroNectin (Takara)-coated plates for 45 min at 4000 rpm at 32°C ...
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bioRxiv - Immunology 2022Quote: ... pMYS-HDAC3(Y298F)-iresGFP were centrifuged onto RetroNectin (Takara)-coated plates for 2 hours at 2000 g at 32°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Predicted enhancer elements were PCR-amplified from mouse genomic DNA (Clontech) and cloned into the respective LacZ expression vector (Osterwalder et al ...
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bioRxiv - Developmental Biology 2022Quote: ... and anti-dsRed (632496, Clontech; 1:300).
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bioRxiv - Genetics 2022Quote: ... and then used to synthesize cDNA with TaKaRa RNA PCR™ Kit (AMV) Ver.3.0 (TaKaRa). Quantitative real-time PCR (qPCR ...
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bioRxiv - Genetics 2022Quote: ... the ptetO7-ALA1 strain was transformed with a vector (pAG425) to express wild-type or mutant AARS1 and grown on media lacking leucine (DO Supplement -Leu, Takara Bio). One colony was picked ...
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bioRxiv - Genetics 2022Quote: ... NG-ABE8e was digested by NotI and EcoRV and subcloned into the SpG-ABE8e plasmid backbone by In-Fusion cloning (Takara Bio, Mountain View, CA). SgRNA-A6 and sgRNA-A8 targeting the A→G R560C mutation site on exon 13 of Pde6b gene in the mouse genome were designed by an online webtool (https://benchling.com) ...
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bioRxiv - Genetics 2022Quote: ... RNA was reverse transcribed to cDNA using the PrimeScript™ RT reagent Kit with gDNA eraser (Takara, Kusatsu, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... and an extra 15 bp at the 3’ and 5’ ends allowing their insertion into the linearized NheI-PacI pUASt-5C plasmid using In-Fusion cloning strategy (Takara Bio USA, Inc.).
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bioRxiv - Cell Biology 2022Quote: ... and a separate master mix was prepared for each primer set using the TB Green® Premix Ex Taq II (TLi RNaseH Plus) reagent kit (RR820A, Takara Bio). The final master mix contained primers at a concentration of 0.4 µM and a 1X concentration of TB Green® Premix Ex Taq II (TLi RNaseH Plus) ...
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bioRxiv - Cell Biology 2022Quote: ... monoclonal anti-GFP JL-8 (1:1000, Clontech 632380), polyclonal rabbit anti-RP2 (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were treated with the appropriate chemicals at the following concentrations: 1 µg/ml doxycycline (Clontech, 631311), 2.5 µM MG132 (APExBIO ...
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bioRxiv - Cell Biology 2022Quote: ... Lentivirus was concentrated using the Lenti-X concentrator (TaKaRa). Cells were selected for six days in puromycin followed by three days induction with doxycycline ...
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bioRxiv - Cell Biology 2022Quote: ... pVSV-G (631530, Clontech, Mountain View, CA) and psPAX2 ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells stably expressing the tetracycline-controlled transactivator protein (Tet-ON, Clontech) were cultured in DMEM with 10% FBS and grown at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... the CMV promoter from pEGFP-C1 (Takara Bio Inc) was removed and replaced with the SEPTIN2 promoter using AseI and AgeI ...
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bioRxiv - Cell Biology 2022Quote: HeLa Tet-ON (Clontech) cells were transfected using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... and used for cDNA synthesis with the PrimeScript RT reagent Kit (Takara Bio). All procedures were performed in accordance with the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... from transformed Stellar Competent Cells (Takara Bio Inc, Japan). The DNA was sequenced and verified (CLC Sequence Viewer 8 ...
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bioRxiv - Cell Biology 2022Quote: ... Immortalization of the cells was achieved by lipofection of the pCDNA-3xHA-hTERT plasmid with Xfect (Takara BIO INC ...
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bioRxiv - Biochemistry 2022Quote: ... encoding a KkRseP derivative having an internal PA14 tag: Lys54-EGGVAMPGAEDDVV-His55) was constructed by using the In-Fusion HD Cloning Kit (Takara Bio) as follows ...
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bioRxiv - Biochemistry 2022Quote: ... W498C and W498R were cloned into pEGFP (Clontech) and/or pRFP (Clontech ...
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bioRxiv - Biochemistry 2022Quote: ... pombe Wsp1p was amplified from genomic DNA and inserted into the pGEX-6P vector via In-Fusion cloning (Takara Bio USA). This vector added an N-terminal GST tag with a precision protease recognition site ...
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bioRxiv - Biochemistry 2022Quote: ... was ligated between NotI and BamHI restriction sites of pQCXIP (Clontech).
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bioRxiv - Biochemistry 2022Quote: ... The elute was diluted such that no more than 1.5ug of DNA was used per PCR reaction and amplified for 20 cycles of PCR using Primestar GXL (Takara Bio), run on a 1% agarose gel ...
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bioRxiv - Biochemistry 2022Quote: ... and mixed with 50 μL TALON Co2+ resin pre-equilibrated in binding buffer A (Takara Bio, 50% slurry) resulting in a final volume of 100 μL ...
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bioRxiv - Biochemistry 2022Quote: ... at the NotI/BamHI sites or into 8xHis-MBP pFastBac1 modified with a CMV promoter (obtained from David Julius’ lab) at the BamHI/NotI sites using In-Fusion EcoDry cloning (Takara) according to manufacturer protocols ...
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bioRxiv - Cell Biology 2022Quote: ... reverse transcription and cDNA synthesis were performed using the SMART-Seq v4 Ultra Low RNA Kit for the Fluidigm C1 System (Takara) reagents ...
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bioRxiv - Cell Biology 2022Quote: ... For each specimen 500 ng of total RNA was reversed transcribed into cDNA using PrimeScript RT reagent kit (TaKaRa RR037a) and oligo dT primers according to the manufacturer’s protocol ...