Labshake search
Citations for Takara Bio :
7051 - 7100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... linearized ToxoXpress vector (pTXP) using In-Fusion HD cloning kit (TaKaRa) as per manufacturer’s protocol (Figure S3 ...
-
bioRxiv - Microbiology 2022Quote: ... 1µl of cDNA was amplified using Advantage HF 2 DNA polymerase (Takara) for 30 cycles according to the manufacturer’s instructions (forward primer ...
-
bioRxiv - Genomics 2022Quote: ... 0.1 μl Ex Taq (Takara, cat# RR001A), 4.3 μl H2O ...
-
bioRxiv - Genomics 2022Quote: ... 0.075 μl (0.375 units) of Ex Taq (Takara), 5 μl of the fragmented DNA ...
-
bioRxiv - Genomics 2022Quote: ... For the Rubicon Genomics ThruPLEX® DNA-seq Kit: High Performance Library Preparation for Illumina® NGS Platforms (currently available from Takara Bio USA), the manufacturer’s protocol was followed.
-
bioRxiv - Microbiology 2022Quote: ... The thrombin-digested recombinant NA was incubated with TALON metal affinity resin (Takara) for 2 h ...
-
bioRxiv - Microbiology 2022Quote: ... All PCRs were performed using PrimeSTAR Max polymerase (Takara Bio) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... column was equilibrated with ten column volumes of Equilibration Buffer (HisTALON Buffer Set, Clontech Laboratories, Cat# 635651). The filtered supernatant was loaded onto the HisTALON cartridge (Clontech Laboratories ...
-
bioRxiv - Microbiology 2022Quote: ... The filtered supernatant was loaded onto the HisTALON cartridge (Clontech Laboratories, Cat# 635683) column at a speed of 1 mL/min ...
-
bioRxiv - Microbiology 2022Quote: ... All PCRs were performed using PrimeSTAR Max polymerase (Takara Bio) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... cDNA was synthesized using a PrimeScript RT Reagent Kit with gDNA Eraser (Takara). The RT-qPCR assay was performed using the TB Green Fast qPCR Mix (Takara) ...
-
bioRxiv - Microbiology 2022Quote: ... HA1 and HA2 primers were designed to contain ∼15 bp overhangs for insertion into pRSng using ligation-independent cloning (LIC) methods (In-Fusion, Clontech). pRSng plasmid was digested with NotI for insertion of HA1 ...
-
bioRxiv - Genetics 2022Quote: ... and 2 ng of total RNA was amplified with SMART-Seq v4 Ultra Low Input RNA kit (Clontech; version “091817”). Sequencing libraries were prepared with NEBnext Ultra DNA Library Prep Kit (New England Biolabs ...
-
bioRxiv - Microbiology 2022Quote: ... covering the full-length SARS-CoV- 2 genome were amplified by PCR using a PrimeSTAR GXL DNA polymerase (TaKaRa Bio), the synthesized cDNA and specific primer sets from CoV-2-G1-Fw to CoV-2-G10-Rv designed previously (21) ...
-
bioRxiv - Microbiology 2022Quote: ... and subjected to cDNA synthesis using a PrimeScript RT reagent kit (Perfect Real Time) (TaKaRa Bio) and random hexamer primers ...
-
bioRxiv - Microbiology 2022Quote: ... The 5 mL HisTALON cartridge (Clontech Laboratories, Cat# 635683) column was equilibrated with ten column volumes of Equilibration Buffer (HisTALON Buffer Set ...
-
bioRxiv - Microbiology 2022Quote: ... cDNA was produced by using Moloney murine leukemia virus (Mo-MLV) reverse transcriptase (TaKaRa, Japan) with a reverse primer ...
-
bioRxiv - Microbiology 2022Quote: ... Quantitative real-time reverse transcription-PCR (qRT-PCR) was performed using the TB Green Premix Ex Taq II (TaKaRa, Japan) with the LightCycler® 96 system (Roche ...
-
bioRxiv - Microbiology 2022Quote: ... The PrimeScript™ RT Reagent Kit (TaKaRa, Japan) was used to reverse-transcribe cDNA from total RNA ...
-
bioRxiv - Genetics 2022Quote: ... cDNA synthesis and adapter ligation were performed with the SMARTer cDNA synthesis kit (Takara Bio). 51bp paired-end reads were generated on an Illumina HiSeq2000 sequencer ...
-
bioRxiv - Genetics 2022Quote: ... The RNA was isolated and was reverse transcribed into cDNA using cDNA synthesis Kit (Takara). The quantitative PCR was done using power SYBR Green PCR master mix (Invitrogen) ...
-
bioRxiv - Genetics 2022Quote: ... The cassette was inserted into MluI restriction site of the pR26 CAG AsiSI/MluI plasmid (addgene 74286, a kind gift from Ralf Kuehn 48) by infusion cloning (Takara). The coding sequence of TIR1-9myc was separated from the CAG promoter by a loxp-STOP-loxp (LSL ...
-
bioRxiv - Genetics 2022Quote: ... using the Infusion assembly protocol (TaKaRa Bio). The sequence of the resulting guide gRNA plasmid was confirmed by sequencing with either T7 or SP6 primers.
-
bioRxiv - Genetics 2022Quote: ... The protein-coding genomic sequence including 3 kbp promoter sequence was PCR-amplified and cloned into the pGreen0029 binary vector (Hellens et al., 2000) using In-Fusion Cloning system (Takara Bio Europe ...
-
bioRxiv - Biochemistry 2022Quote: ... GFP-P2A-poly-K and GFP-P2A-102×PR were cloned by restriction digestion and ligation and inserted into pLVX-TetOne-Puro vector (Takara Bio # 631849) using Gibson assembly ...
-
bioRxiv - Biochemistry 2022Quote: ... Library was prepared using SMARTer smRNA-seq Kit for Illumina (Takara, 635029) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2022Quote: ... Mighty Mix T4 DNA ligase from Takara; Dual luciferase reporter assay from Promega ...
-
bioRxiv - Genetics 2022Quote: ... then combined with 6 µL cDNA and 15 µL Mighty Mix T4 DNA ligase reaction mix (Takara) and incubated overnight at 16 °C ...
-
bioRxiv - Microbiology 2022Quote: ... The RT-qPCR assay was performed using the TB Green Fast qPCR Mix (Takara). The ribosomal protein L21 gene (RPL21 ...
-
bioRxiv - Developmental Biology 2022Quote: ... Lentivirus-containing medium was concentrated using Lenti-X-concentrator (Takara Bio). Six-well plates were seeded with 100,000 primary keratinocytes per well and transduced with 150 uL each of lenti-eGFP ...
-
bioRxiv - Developmental Biology 2022Quote: ... and IFT20 floxed mice using TRIzol reagent (TaKaRa, Japan) was reverse-transcribed into cDNA by PCR using PrimeScript™ RT Kit (TaKaRa ...
-
bioRxiv - Developmental Biology 2022Quote: ... was reverse-transcribed into cDNA by PCR using PrimeScript™ RT Kit (TaKaRa, Japan). Then ...
-
bioRxiv - Developmental Biology 2022Quote: ... Genomic DNA was purified from a small section of embryos using SimplePrep™ reagent for DNA (TaKaRa Bio, Kusatsu, Japan).
-
bioRxiv - Cell Biology 2022Quote: ... pNFLS2,51,55,66A-myc and pNFLS2,55,57,66D-myc constructs and then cloned these sequences into the pEGFP-N3 vector (Clontech/Takara Bio) between the EcoR1 and BamH1 restriction sites of the multiple cloning site ...
-
bioRxiv - Cell Biology 2022Quote: ... E.coli competent cells transformed with pTf16 chaperone protein tag (TaKaRa Bio) per the manufacturer’s protocol.
-
bioRxiv - Evolutionary Biology 2022Quote: ... with the chaperone plasmid pG-KJE8 (Takara Bio Inc.), was selected for AnMurE_pPROEX ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... single-stranded cDNA was generated from RNA using the SMARTer cDNA synthesis kit (Clontech, Palo Alto, CA) with tagged oligo-dT primers that include one of eight 15-bp barcodes to distinguish individual samples (2 species x 2 tissues x 2 biological replicates) ...
-
bioRxiv - Microbiology 2022Quote: Total RNA from mouse corneas was extracted by the RNAiso plus reagent (TaKaRa, Dalian, China). RNA samples were reverse transcribed using HiScript III RT SuperMix (Vazyme ...
-
bioRxiv - Microbiology 2022Quote: ... expression was induced by addition of 4 ng/ml anhydrotetracycline (Clontech) at 4 hpi.
-
bioRxiv - Microbiology 2022Quote: ... the supernatant was collected and concentrated by Lenti-X™ Concentrator following the manufactures protocol (Clontech, CA). The pellet was resuspended in PBS ...
-
bioRxiv - Microbiology 2022Quote: ... which are derivatives of pEGFPC1 and pEGFPC2 (Clontech), was described in previous publications (Rzomp et al. ...
-
bioRxiv - Genetics 2022Quote: ... Each 30 µL PCR reaction volume contained 15 µL Premix Ex Taq enzyme (Takara Biomedical Technology, Beijing, China), 0.2 µL bovine serum albumin (BSA) ...
-
bioRxiv - Genetics 2022Quote: ... Each 30 µL PCR reaction volume contained 15 µL Premix Ex Taq enzyme (Takara Biomedical Technology), 0.2 µL BSA ...
-
bioRxiv - Genetics 2022Quote: ... The 20 µL PCR reaction volume included 10 µL Premix Ex Taq enzyme (Takara Biomedical Technology), 0.2 µL BSA ...
-
bioRxiv - Biochemistry 2022Quote: ... DNA-lipid complexes were prepared by incubating 100 ng of eGFP-N1 plasmid (Takara Biosciences) with Lipofectamine 2000 for 20 min ...
-
bioRxiv - Neuroscience 2022Quote: ... and anti-GFP 1:1000 (Clontech, 632380), anti-α-syn 1:1000 (C-20 ...
-
bioRxiv - Microbiology 2022Quote: ... HeLa Tet-Off cells (Clontech), Atg5 +/+ and -/- MEFs (119) ...
-
Map7D2 and Map7D1 facilitate microtubule stabilization through distinct mechanisms in neuronal cellsbioRxiv - Cell Biology 2022Quote: ... and pEGFP-N3 (Clontech). GST-fused proteins were expressed in Escherichia coli and purified using glutathione-Sepharose beads (Cytiva) ...
-
bioRxiv - Cell Biology 2022Quote: ... RT reaction was performed with SmartScribe enzyme (SMARTScribe Reverse Transcriptase, Clontech) at 42°C for one hour followed by enzyme inactivation at 70°C for 15 minutes ...
-
bioRxiv - Plant Biology 2022Quote: ... Quantitative PCR reactions were performed using the Takara TP-850 thermal cycler (Takara Bio, Japan, www.takara-bio.com) and THUNDERBIRDTM SYBR® qPCR Mix from Toyobo (https://www.toyobo.co.jp) ...