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Citations for Takara Bio :
7001 - 7050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... then concentrating the supernatant with AAVpro® concentrator (TaKaRa).
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bioRxiv - Neuroscience 2022Quote: ... pEGFP-C3 (Clontech, Mountain View, CA, USA), and pFLAG-CMV2 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... The genes were cloned into the pCold III–Mor1 expression vector between the SacI (Takara Bio) and XbaI (Takara Bio ...
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bioRxiv - Microbiology 2022Quote: ... and 4 μM random hexamer primers (Takara Bio ...
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bioRxiv - Neuroscience 2022Quote: ... spent medium was collected and filtered through a 0.45 μm cellulose acetate filter before concentrating the supernatant using Lenti-X™ concentrator (TaKaRa).
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bioRxiv - Developmental Biology 2022Quote: ... cDNA libraries were prepared using SMART-seq v4 Ultra Low Input RNA Kit for Sequencing (TaKaRa Bio), which is based on the SMART-seq2 method (Picelli et al. ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was reverse transcribed using oligo(dT) primers with the SMARTer Ultra Low Input system (Takara). The cDNA was then sheared with a Covaris S2 ultrasonicator ...
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bioRxiv - Microbiology 2022Quote: ... using the SMARTer ThrupPLEX DNA-Seq kit (R400676, Takara-Clontech). Briefly ...
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bioRxiv - Microbiology 2022Quote: All plasmid inserts were amplified by PCR using standard PCR conditions and the CloneAmp HiFi PCR premix (Takara). Following a PCR purification step (QIAquick PCR purification kit) ...
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bioRxiv - Bioengineering 2022Quote: ... A lentiviral transfer plasmid co-encoding zsGreen with Oatp1b1 was cloned using the In-Fusion HD Cloning kit (Takara Bio USA Inc ...
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bioRxiv - Biochemistry 2022Quote: Co2+-bound NTA-sepharose beads (Takara) were stripped off the metal ion with 100 mM EDTA and used as NTA beads or charged with 100 mM NiCl2 and used as NTA(Ni2+ ...
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bioRxiv - Cell Biology 2022Quote: ... pNFLS2,51,55,66A-myc and pNFLS2,55,57,66D-myc constructs and then cloned these sequences into the pEGFP-N3 vector (Clontech/Takara Bio) between the EcoR1 and BamH1 restriction sites of the multiple cloning site ...
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bioRxiv - Developmental Biology 2022Quote: ... ESCs were co-transfected with 2μg of this vector and 0,12μg of an hygromycin marker (#631625, TAKARA) using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... The RNA-seq library was prepared with SMARTer® Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian (Takara), and sequencing was performed in the Next Generation Sequencing platform using NextSeq500 (Illumina) ...
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bioRxiv - Genomics 2022Quote: ... and 0.19 U RNase inhibitor (Clontech, 2313A)) was added to each well ...
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bioRxiv - Genomics 2022Quote: RNA sequencing libraries were prepared from exRNAs isolated using the exoRNeasy and ExoTIC EV isolation methods from adherent monolayer H358 cells using SMART-Seq (Takara Bio) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: The sequence of anti-IFNγ was cloned from XMG1.2 hybridoma (ATCC) using SMARTer RACE 5’/3’ kit (Takara Bio). The 6xHis tagged anti-Dsg ...
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Effects of Sanhuang plaster on expression of MyD88, TRAF6, MIP-1β and IL-23 in rats infected by MRSAbioRxiv - Molecular Biology 2022Quote: ... Real-time fluorescence quantitative PCR kit and reverse transcription kit(TaKaRa Co., Ltd.); MyD88 Anti-body TRAF6 Anti-body MIP-1β Anti-body and IL-23 Anti-body (GeneTexCo. ...
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bioRxiv - Molecular Biology 2022Quote: ... The conventional PCR was performed separately for the genes in a 25µl volume with 12.5µl 2x Emerald GT amp master mix (TaKaRa, Cat# RR310A) along with 10 picomole of each of the forward and reverse primers for the respective genes ...
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bioRxiv - Molecular Biology 2022Quote: ... Probe qPCR master mix (TaKaRa, Japan) was used for the quantitative analysis of COWP and 18ssu rRNA genes ...
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bioRxiv - Microbiology 2022Quote: ... GFP (Clontech), PKR ...
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bioRxiv - Microbiology 2022Quote: ... using the SMARTer ThrupPLEX DNA-Seq kit (R400676, Takara-Clontech). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... the fragments were sequentially ligated into the target vector using the In-Fusion HD Cloning Kit (Clontech). The resulting plasmid sequences were verified by Sanger sequencing (GATC Biotech ...
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bioRxiv - Microbiology 2022Quote: ... was performed with one-step Prime script III RT-qPCR mix (Takara, Japan). The viral RNA of NP was detected by 2019-nCoV-N1 probe (Cat#10006770 ...
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Core Microbiota Drive the Prevalence of Extracellular Antibiotic Resistome in the Water CompartmentsbioRxiv - Microbiology 2022Quote: ... High throughput qPCR (HT-qPCR) was implemented on SmartChip PCR system including a Multisample Nanodispenser and a Cycler (Takara SmartChip™ ...
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bioRxiv - Microbiology 2022Quote: ... The pCMV-GFP vector was obtained from Clontech. Transfections were performed using jetPRIME Transfection-Reagent (Polyplus-transfection) ...
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bioRxiv - Microbiology 2022Quote: The pCold III –Mor1 expression vector was constructed from pCold III vector (Takara Bio, Kyoto, Japan) at the NgoMIV (NEB ...
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bioRxiv - Immunology 2022Quote: ... First-strand cDNA was generated from 1 μg of RNA for each sample using the SMARTer RACE 5’/3’ Kit (Takara Bio ...
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bioRxiv - Immunology 2022Quote: ... Rearranged VDJ IGM and IGG amplicons were generated using a universal forward primer (Takara Bio;10 μM), and either an IgM-CH3 (5’-CAGATCCCTGTGAGTCACAGTACAC-3’ ...
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bioRxiv - Immunology 2022Quote: ... or splenocytes from a naïve C57BL/6 mouse using NucleoSpin RNA Plus (Takara). RNA was reverse transcribed with SuperScript III RT and random primers (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: All restriction enzymes were purchased from TaKaRa Bio or Minotech (Lab Supplies Scientific SA ...
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bioRxiv - Microbiology 2022Quote: ... An internal ribosomal entry site (IRES) was inserted behind the ACE2 sequence via restriction digestion of a pEF1a-IRES vector (Cat. n° 631970, Takara Bio Inc.) with NheI-HF and SalI-HF (NEB) ...
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bioRxiv - Microbiology 2022Quote: ... X-alpha-Gal (X-α-gal) and aureobasidin A (AbA) were obtained from TaKaRa. Plasmids ...
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bioRxiv - Microbiology 2022Quote: ... Each of these prey plasmids and previously constructed bait plasmids were co-transformed into Y2HGold yeast strain using Yeastmaker Yeast Transformation System 2 (TaKaRa). The transformation mix was spread on DDO/X and QDO/X/A agar plates and incubated at 30°C for 3-5 days ...
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bioRxiv - Microbiology 2022Quote: ... treatment was used for cDNA library preparation using Make Your Own “Mate & Plate™” Library System (TaKaRa). All the procedures were followed according to the manual provided ...
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bioRxiv - Microbiology 2022Quote: The yeast-two-hybrid library screening was performed according to the protocols of the Matchmaker® Gold Yeast Two-Hybrid System (TaKaRa). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... the cDNA was amplified by long distance PCR amplification using Advantage polymerase mix (TaKaRa). The amplified ds cDNA was purified using CHROMA SPIN TE-400 column which retains and traps the DNA molecules of less than 200 bp size ...
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bioRxiv - Microbiology 2022Quote: ... Quantitative real-time RCR (qRT-PCR) was carried-out using SYBR Premix Ex Taq™ (Tli RNaseH Plus) (TaKaRa, Japan) and CFX96TM real-time PCR system (Biorad ...
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bioRxiv - Microbiology 2022Quote: ... The cDNA was prepared from 1 μg of total RNA using PrimeScript 1st strand cDNA Synthesis Kit (TaKaRa, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Make Your Own “Mate & Plate™” Library System and Yeast Transformation System 2 were purchased from TaKaRa. Synthetic-defined (SD ...
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bioRxiv - Microbiology 2022Quote: ... The purified ds cDNA and SmaI linearized pGADT7-Rec were co-transformed into Y187 yeast strain using Yeastmaker Yeast Transformation System 2 (TaKaRa). The transformation mix was spread on to the SD/-Leu agar plates and incubated at 30°C for 3–4 days ...
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bioRxiv - Microbiology 2022Quote: ... The plasmids were extracted from all the colonies which grew on QDO/X/A agar plate using Easy Yeast Plasmid Isolation Kit (TaKaRa). PCR was carried out using the extracted plasmids as template ...
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bioRxiv - Microbiology 2022Quote: ... genomic DNA was digested with S1 nuclease (TaKaRa, Kusatsu, Japan) at 37°C for 20 minutes ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA loads in samples were measured using Premix Ex Taq (probe qPCR; Takara) with primers and probes as follows ...
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bioRxiv - Microbiology 2022Quote: ... Differential regulation of cellular genes was assessed using TB Green™ Premix Ex Taq™ II (Tli RNase H Plus; Takara) according to manufacturer’s instructions with QuantiTect primer assays (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... RNA was reverse transcribed with the prime Script RT reagent kit (Takara). Differential regulation of cellular genes was assessed using TB Green™ Premix Ex Taq™ II (Tli RNase H Plus ...
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bioRxiv - Microbiology 2022Quote: ... and Ex Taq DNA Polymerase (TaKaRa Bio, Cat.:RR001), to add sequencing indexes to each amplicon ...
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bioRxiv - Microbiology 2022Quote: ... Samples showing successful RNA extraction after checking by Bioanalyzer electrophoresis were reverse transcribed and cDNA amplified using the SmartSeq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara Bio. Inc., Shiga Prefecture, Japan). Successfully amplified samples were prepared for paired end short-read sequencing using the Nextera XT Library Prep kit (Illumina ...
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bioRxiv - Biochemistry 2022Quote: The resulting cDNAs were amplified by PCR using LA Taq polymerase (Takara), and the PCR reactions were then treated with 200 U/mL of Exonuclease I (NEB ...
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bioRxiv - Microbiology 2022Quote: ... the Lenti-X CRISPR/Cas9-System (Takara) was used ...