Labshake search
Citations for Takara Bio :
6951 - 7000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 1% PS and doxycycline (1 μg/ml; Takara, Cat# 1311N). At six days posttransfection ...
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bioRxiv - Microbiology 2022Quote: ... GISAID ID: EPI_ISL_408667)29 were prepared by PCR using PrimeSTAR GXL DNA polymerase (Takara, Cat# R050A). A linker fragment encoding hepatitis delta virus ribozyme ...
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bioRxiv - Microbiology 2022Quote: ... a set of primer/probe E484A (SARS-CoV-2) (Takara, Cat# RC322A) was used ...
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bioRxiv - Microbiology 2022Quote: ... the CPER products were transfected into HEK293-C34 cells using TransIT-LT1 (Takara, Cat# MIR2300) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA from each sample was amplified using the Takara SMART-Seq HT Kit (Takara) according to manufacturer’s instructions and run for 20 cycles ...
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bioRxiv - Molecular Biology 2022Quote: ... The plasmid DNA bearing the chaperone combination GroEL/ES (pGro7) or DnaK/J/GrpE (pKJE) from Takara Biotech chaperone kit [Nishihara et al. ...
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bioRxiv - Molecular Biology 2022Quote: Plasmids were generated using the Infusion method (Takara Bio USA, Inc.). PCR fragments were amplified using PrimerStar (normal PCR or site-directed mutagenesis ...
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bioRxiv - Microbiology 2022Quote: ... following PCR-amplification using primer pairs TgCERS1.CFE.P1 / TgCERS1.CFE.P2_FLAG and TgCERS2.CFE.P1 / TgCERS2.CFE.P2_FLAG respectively (Table S3) and InFusion (TaKaRa) cloned into pEU-E01-MCS ...
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bioRxiv - Microbiology 2022Quote: ... Fragments were cloned into a PvuII linearized pUC19 using In-Fusion HD cloning kit (TaKaRa) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... All the reactions were performed in duplicates in Premix Ex Taq Probe qPCR Master Mix (2X) (TaKaRa, Japan Cat#RR390) in a final volume of 25µl per tube in a CFX96 Real time PCR system® (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... we used the Matchmaker® Gold Yeast One-Hybrid Library Screening System (Clontech, Mountain View, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... was purchased from Takara Bio Inc ...
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bioRxiv - Molecular Biology 2022Quote: The quantified RNA was converted into copy DNA (cDNA) using Primescript™ 1st strand cDNA synthesis kit (TaKaRa, Japan). 1st Reaction Mixture was prepared by adding 8.0 µl of RNA (1.0μg/concentration adjusted to 125.0ng/µl) ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 10% FBS or Tet-system approved FBS (Takara Bio), 1% P/S ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA (500 ng) from the worms was reverse transcribed into cDNA by using a PrimeScript RT Reagent Kit (TaKaRa, Dalian, China) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Prime STAR® HS (Premix) (Takara Bio Inc., Shiga, Japan) was used for PCR amplification ...
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bioRxiv - Microbiology 2022Quote: ... Quantitative real-time PCR was done using SYBR/ TB green (TAKARA) RT-PCR kit in BioRad qRT-PCR system ...
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bioRxiv - Microbiology 2022Quote: ... the cDNA was synthesized by a cDNA synthesis kit (Takara). Quantitative real-time PCR was done using SYBR/ TB green (TAKARA ...
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bioRxiv - Microbiology 2022Quote: ... cells were transduced in 24-well plates precoated with recombinant fibronectin (FN CH-296, Retronectin Takara, Clontech, Mountain View, CA) with retroviral supernatants and expanded in complete medium (45% RPMI-1640 and 45% Click’s medium ...
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bioRxiv - Microbiology 2022Quote: ... and A549-MxB(1-715)-MxB(26-715) cells expressing untagged MxB(1-715) and MxB(26-715) using the pLKOD-Ires-Puro vector (Clontech Takara Bio, Mountain View, United States). MeWo cells (kind gift from Graham Ogg ...
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bioRxiv - Neuroscience 2022Quote: ... with either 7.5 μg of pcDNA3.1 and pcDNA3.1-p27-Flag (p27 full-length construct) or 7.5 μg of empty pMSCVpuro (Clontech, 634401) and pSUPER.retro mSox2.3 (shRNASox2 target sequence ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting cDNA was subjected to relative quantitative PCR using a SYBR Premix Ex Taq™ kit (TaKaRa) on a Roche LightCycler 480 real-time PCR machine ...
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bioRxiv - Molecular Biology 2022Quote: ... Use the One Step TB Green® PrimeScript™ PLUS RT-PCR Kit (Takara) to quantify the viral RNA copies through the CFX96 Touch Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was synthesized by the use of PrimeScript™RT reagent Kit with gDNA Eraser (Perfect Real Time) according to the reverse transcription protocol (Takara). The resulting cDNA was subjected to relative quantitative PCR using a SYBR Premix Ex Taq™ kit (TaKaRa ...
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bioRxiv - Microbiology 2022Quote: ... PreS2-LHBS sequence (deletion nucleotides 2-55) was cloned into the protein stability construct after the c-terminal of EGFP by In-Fusion cloning (Takara). The protein stability construct was a kind gift from Dr ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-mCherry (Takara Bio ...
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bioRxiv - Microbiology 2022Quote: ... Mink ACE2 full length gene was amplified using long range PCR (LRPCR) amplification assay in two overlapping fragments using high-fidelity PrimeSTAR GXL DNA Polymerase (Takara Bio USA) as previously described (34,35) ...
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bioRxiv - Microbiology 2022Quote: ... The resulting supernatant was supplemented with 0.75 mL pre-equilibrated TALON resin (Takara Clontech) and 1 mM imidazole and stirred for 30 min at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... The resulting supernatant was supplemented with 0.75 mL pre-equilibrated TALON resin (Takara Clontech) and 1 mM imidazole and stirred for 30 min at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... and 1.25 units of PrimeSTAR GXL DNA Polymerase (Takara Bio USA, Inc., San Jose, CA). The LRPCR mixture was incubated at 98°C for 2 minutes for the initial denaturation ...
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bioRxiv - Microbiology 2022Quote: ... The In-fusion HD Cloning Plus kit was purchased from Takara Bio USA ...
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bioRxiv - Microbiology 2022Quote: ... Tick DNA was extracted using the MightyPrep reagent for DNA Kit (Takara, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Samples were analyzed using a One-Step SYBR PrimerScript reverse transcription (RT)-PCR kit (TaKaRa, Japan) on Applied Biosystems QuantStudio ...
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bioRxiv - Microbiology 2022Quote: ... and the mRNA was then reverse transcribed into cDNA (Takara, Beijing). qRT-PCR was then performed using a Bio-Rad CFX-96 PCR detection system (USA ...
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bioRxiv - Microbiology 2022Quote: ... and A549-MxB(1-715)-MxB(26-715) cells expressing untagged MxB(1-715) and MxB(26-715) using the pLKOD-Ires-Puro vector (Clontech Takara Bio ...
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bioRxiv - Microbiology 2022Quote: ... qRT-PCR was then performed using a Bio-Rad CFX-96 PCR detection system (USA) and a SYBR Green I mixture (Takara, Beijing). The MRSA housekeeping gene gyrB was used as an internal reference for normalization of the data.
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bioRxiv - Molecular Biology 2022Quote: ... or TALON metal affinity resin (Takara), for GST-tagged and His-tagged constructs ...
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bioRxiv - Microbiology 2022Quote: The recombinant pGBKT7-TaPHB-1 was transformed into Y2HGold yeast strain following the Yeastmaker Yeast Transformation System 2 (TaKaRa). The transformants were screened on SD/-Trp agar plate ...
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bioRxiv - Neuroscience 2022Quote: ... Constructs for human Tara were prepared by cloning full-length TRIOBP1 (Trio and F-actin binding protein1) isoform into pEGFP-C3 (Clontech, Mountain View, CA, USA), pFLAG-CMV2 (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... AAV11 Cap sequence retrieved on Genbank (accession number: AY631966.1) was synthesized as a template for amplifying AAV11 Cap fragment using PrimeSTAR HS DNA Polymerase (Takara, R040A), After being digested by using SwaI and AgeI (New England Biolabs ...
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bioRxiv - Neuroscience 2022Quote: ... total RNA was reverse transcribed to cDNA using the PrimeScript™ RT reagent Kit (TaKaRa, RR037A, Japan). Convergent and divergent primers were used to detect the expression of linear and circular RNA transcripts ...
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bioRxiv - Neuroscience 2022Quote: ... These RNA samples were then reverse transcribed into cDNA by PrimeScript™ RT Reagent (TaKaRa, RR036A, Japan). Real-time polymerase chain reaction (RT-PCR ...
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bioRxiv - Neuroscience 2022Quote: ... from purified RNA (NucleoSpin RNA kit; Takara). Raw data from the QuantStudio 5 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted from tissues and cells with TRIzol reagent (TaKaRa, Japan), and genomic DNA was isolated using a TIANamp Genomic DNA Kit (TianGen Biotech ...
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bioRxiv - Developmental Biology 2022Quote: ... a full-length Dunk was used to screen the Mate & Plate™ Library - Universal Drosophila (Normalized) (Takara, 630485). This genome-wide Drosophila cDNA library is prepared from equal quantities of poly-A+ RNA isolated from embryo ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR polymerase capable of handling GC-rich amplicons was used (PrimeSTAR GXL Premix, Clontech). The resulting DNA amplicon ...
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bioRxiv - Developmental Biology 2022Quote: ... The screen was performed followed by the Matchmaker Gold Yeast Two-Hybrid System User Manual (Takara, 071519). One large Y2HGold[pGBKT7-Dunk] yeast colony was picked from a fresh SD/-Trp plate and inoculated into 50 ml of SD/-Trp liquid media ...
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bioRxiv - Developmental Biology 2022Quote: ... The expression of Dunk protein was confirmed by Western blot using c-Myc Monoclonal Antibody (Takara, Cat# 631206). Then ...
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bioRxiv - Neuroscience 2022Quote: ... and fresh 0.2% RNase inhibitor (Takara Bio, Cat# 2313A)) for 15 minutes ...
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bioRxiv - Neuroscience 2022Quote: Between 1-3 ng of the nuclear RNA was used in the SMART-seq v4 Ultra Low Input RNA Kit for Sequencing (Takara, cat # 634888) according to the manufacturer’s protocols ...