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Citations for Takara Bio :
6901 - 6950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... in a 25 µl reaction volume with buffer and dNTPs provided with the PrimeSTAR GXL polymerase (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... and 3’ UTR region was cloned into the PacI site in pZH_OsU6sgRNA_SpCas-wt (Endo et al., 2019) using an In-Fusion HD cloning kit (Takara). Two pairs of 20-nt annealed oligonucleotides targeting exon 4 of the OsSOG1 gene were cloned into the BbsI site of the pU6gRNA-oligo (pU6::sgOsSOG1-2 and pU6::sgOsSOG1-3 ...
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bioRxiv - Plant Biology 2022Quote: ... PCR fragments representing either the OsSOG1 promoter+GUS gene or OsSGL promoter+GUS gene were inserted into the AscI/AvrII site using an In-Fusion HD cloning kit (Takara) between attL1 and T35S in pE(L1-L2)hpt ...
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bioRxiv - Plant Biology 2022Quote: ... qRT-PCR was carried out with SYBR Premix Ex Taq II Premix (Takara) on the real-time system (Roche ...
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bioRxiv - Plant Biology 2022Quote: ... One microgram DNAse-treated RNA was converted to cDNA using Prime RT-PCR kit (Takara Bio Inc.). The qRT-PCR was performed on 11 selected DEGs containing DRE elements ...
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bioRxiv - Plant Biology 2022Quote: ... CITRINE-fusion proteins were detected with anti-GFP antibody (JL-8, Takara Bio Clontech, dilution ...
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bioRxiv - Plant Biology 2022Quote: ... The 1 μg total RNAs were used to synthesize cDNA with the PrimeScript™RT reagent Kit with gDNA Eraser (Takara). qRT-PCR was carried out with SYBR Premix Ex Taq II Premix (Takara ...
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bioRxiv - Plant Biology 2022Quote: ... CITRINE-fusion proteins were detected with anti-GFP antibody (JL-8, Takara Bio Clontech ...
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bioRxiv - Plant Biology 2022Quote: ... All yeast experiments were carried out using the Yeast Protocols Handbook (Clontech).
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bioRxiv - Plant Biology 2022Quote: ... 10 μL of SYBR Premix Ex TaqTM II (Takara), and 0.4μL of reference dye ...
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bioRxiv - Plant Biology 2022Quote: ... and OsTPR3 were introduced into the vector pGBKT7 (Clontech) to produce pGBKT7-TAD1 ...
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bioRxiv - Plant Biology 2022Quote: ... The full-length and truncated coding regions of WL1 were amplified and cloned into the yeast expression vector pGADT7 (Clontech) to produce pGADT7-WL1 ...
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bioRxiv - Plant Biology 2022Quote: ... The gRNAs used in this study were synthesized and inserted at BsaI site of pDC expressing vectors using T4 ligase (2011A, Takara). For dual sgRNA system ...
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bioRxiv - Plant Biology 2022Quote: ... and SYBR permix Ex Taq II Kit (TaKaRa, Kyoto, Japan); using actin as an internal control ...
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bioRxiv - Plant Biology 2022Quote: ... The Y2H Gold yeast strain (Clontech) was transformed with appropriate pGADT7 and pGBKT7 constructs ...
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bioRxiv - Plant Biology 2022Quote: ... PCR was conducted using ExTaq (Takara Bio, Kusatsu, Japan) and amplicons were visualized on a 1.4% agarose gel.
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bioRxiv - Plant Biology 2022Quote: ... using an In-Fusion HD cloning kit (Takara), yielding pE(L1-L2)OsSOG1_7Apb ...
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bioRxiv - Synthetic Biology 2022Quote: Pantropic vesicular stomatitis virus G pseudotyped lentivirus was produced via transfection of LentiX 293T cells (Clontech #11131D) with a pHR’SIN:CSW transgene expression vector and the viral packaging plasmids pCMVdR8.91 and pMD2.G using FuGENE HD (Promega ...
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bioRxiv - Systems Biology 2022Quote: ... 0.1μl RNAse inhibitor (40U/μl RRI, TaKaRa)) and immediately stored on dry ice or transferred to a −80°C freezer for long-term storage ...
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bioRxiv - Systems Biology 2022Quote: ... 0.25μl RNAse inhibitor (40U/μl RRI, TaKaRa), 0.25μl SuperScript II reverse transcriptase (200U/μl ...
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bioRxiv - Neuroscience 2022Quote: ... a sequence region of 5,000 bp between SnaBI and AgeI restriction sites was amplified using the HiFi amplification kit (Clontech, Takara Bio Europe ...
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bioRxiv - Systems Biology 2022Quote: ... 0.05μl RNAse Inhibitor (40U/μl RRI, TaKaRa), 0.08μl TSO oligo (100uM ...
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bioRxiv - Systems Biology 2022Quote: ... In brief we sorted labeled T cells into 384 well plates (Armadillo) containing 3μl of Smart-seq3 lysis buffer (0.04μl RNAse inhibitor (40U/μl RRI, TaKaRa), 0.1% Triton X-100 ...
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bioRxiv - Systems Biology 2022Quote: ... and deposited in 10 uL ice-cold lysis buffer containing 0.1% Triton-X 100 and RNase inhibitor (Takara) and stored at −80°C until further processing ...
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bioRxiv - Pathology 2022Quote: ... Single-stranded cDNA was reverse transcribed using Prime Script RT Master Mix (Perfect Real Time) (Takara Bio Inc.), and used for subsequent real-time PCR reactions ...
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bioRxiv - Pathology 2022Quote: ... PCR reactions were conducted with SYBR qPCR Premix Ex Taq II Tli RNaseH+ (TAKRR820W,Takara). Primer pairs are listed in Table S2 ...
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bioRxiv - Pathology 2022Quote: ... cDNA was synthesized using PrimeScript kit (Takara) this was followed by quantitative qPCR using Power SYBR Mix (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The gene strand was inserted into the linearized backbone using InFusion (Takara) following the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... The bait and prey vectors were co-transformed into yeast strain Y2H Gold according to the Yeast Protocols Handbook (Clontech), diluted on SD/-Leu-Trp/AbA for selection of positive colonies ...
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bioRxiv - Pathology 2022Quote: ... 200 ng of total RNA was reverse transcribed using cDNA Synthesis Mix (Takara, Dalian, China). Real-time PCR was carried out in an Applied Biosystems QuantStudio 5 Real-Time PCR System (ABI ...
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bioRxiv - Physiology 2022Quote: ... the cDNAs of full length human GCNA or IDR hGCNA were cloned into pEGFP-C1 (Clontech) between BglII and SalI sites ...
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bioRxiv - Neuroscience 2022Quote: ... We inserted the PCR fragments at NheI/BamHI sites by infusion HD-cloning (Takara Biosciences), to excise HA-tag from the plasmid ...
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bioRxiv - Molecular Biology 2022Quote: ... ceranae-inoculated workers’ midguts at 7 dpi and 10 dpi were respectively isolated using RNA Extraction Kit (TaKaRa company, Dalian, China). cDNA was synthesized through reverse transcription with oligo dT primer and used as templates for qPCR assay ...
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bioRxiv - Neuroscience 2022Quote: ... we carry out all steps at 4°C and in the presence of RNase inhibitor (Takara). We use the isolated nuclei for the droplet-based 10x scRNA-seq assay ...
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bioRxiv - Molecular Biology 2022Quote: ... Splice sites (SS) mutations were introduced in the corresponding parental plasmid using In-Fusion HD Cloning kit (Takara Bio) to generate pcDNA3.1-RLuc_FOXP3 Intron 7 5’-SS G- U ...
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bioRxiv - Neuroscience 2022Quote: ... The 86 bp insert of Mito-DsRed2 (Clontech) was subcloned at the NheI and BsrGI sites of the pCL6EGwo vector using standard recombinant DNA technology (pCL6-Mito-DsRed2) ...
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bioRxiv - Neuroscience 2022Quote: ... cells were plated in 24-well plates and transfected with 1.5 μg/μl of psDsRed-2Mito plasmid (Clontech).
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bioRxiv - Microbiology 2022Quote: ... the ECMV IRES was cloned out of pIRES (Clontech, Mountain View, CA, USA) into pEYFP-N1 30bp upstream of the eYFP ...
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bioRxiv - Microbiology 2022Quote: ... a one-step reverse transcription-PCR (RT-PCR) assay was conducted with a PrimeScript kit (Takara, Tokyo, Japan), and Viral genomic terminal sequences were determined using commercial 5’ and 3’ RACE (rapid amplification of cDNA ends ...
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bioRxiv - Microbiology 2022Quote: ... The viral supernatants were filtered with 0.45 µm pore size and then concentrated using the Lenti-X concentrator (Takara) following manufacturer’s instruction.
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bioRxiv - Microbiology 2022Quote: Sequence of the human variable heavy and kappa chains were obtained by using SMARTer 5’ RACE technology (Takara Bio USA) adapted for antibodies to amplify the variable genes from heavy and kappa chains for each hybridoma ...
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bioRxiv - Microbiology 2022Quote: ... Linearized vector and inserts were ligated using In-Fusion® HD Cloning Kit (Clontech, Takara, Japan) and transformed into StellarTM competent cells ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNAs were amplified using the corresponding primers (Supplementary Table S3) and fused with the DNA-binding or activation domain of GAL4 in the corresponding pGBT9 and pGAD424 vectors (Clontech). Details of assembling the constructs for expressing proteins in transgenic flies are available upon request.
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bioRxiv - Microbiology 2022Quote: ... Amplifying PCR of the first stand cDNA product was then performed using SeqAmp DNA Polymerase (Takara) with a nested 3’ primer to the constant genes and a 5’ universal primer based on universal primer sites added to the 5’ end during cDNA generation ...
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bioRxiv - Microbiology 2022Quote: ... KSHV replication in iSLK/Bac16 cells was reactivated by a combination of 1 μg/ml doxycycline (DOX, Clontech) and 1 mM sodium butyrate (Bu ...
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bioRxiv - Microbiology 2022Quote: ... Viral genomes were extracted from the supernatants at different time points post-infection using Takara MiniBEST Viral RNA/DNA (Takara Bio, Kyoto, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Then RNA was reverse-transcribed and amplified using One Step PrimeScriptTM III RT-qPCR Mix kit (Takara Bio, Kyoto, Japan). Primers and probes ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 cells were cotransfected with the S expression plasmids (400 ng) and pDSP8-11 (400 ng) using TransIT-LT1 (Takara, Cat# MIR2300). To prepare target cells ...
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bioRxiv - Microbiology 2022Quote: ... 0.8 U/μl recombinant RNase inhibitor (Takara, Cat# 2313B)] and incubated at room temperature for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... 1% PS and doxycycline (1 μg/ml; Takara, Cat# 1311N). At 7 days posttransfection ...