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Citations for Takara Bio :
6851 - 6900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with primary antibody (anti-Th [mouse] 1:1500, Immunostar; anti-dsRed [rabbit] 1:1500, Clontech) overnight at RT shaking ...
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bioRxiv - Molecular Biology 2022Quote: ... the larvae were fixed in 4% PFA for imaging or RNAiso Plus (Takara, 9109) for RNA isolation.
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bioRxiv - Plant Biology 2022Quote: ... polymorpha cDNA using PrimeSTAR HS DNA polymerase (Takara Bio) with primers CDS_MpGUN1_At_gib_F and CDS_MpGUN1_At_gib_R ...
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bioRxiv - Neuroscience 2022Quote: ... and VSV-G (viral envelope) (Stewart et al., 2003) into Lenti-X HEK293T cells (Clontech) at a mass ratio of 5:4:3 using polyethyleneimine (PEI) ...
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bioRxiv - Plant Biology 2022Quote: ... Amplified fragments were inserted into the respective vectors by using the In-Fusion enzyme (Takara Bio, USA). For construction of CRISPR/cas9-mediated CLE42 knockout plants ...
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bioRxiv - Plant Biology 2022Quote: ... qPCR was carried out using SYBR Premix Ex Taq II (Takara Bio) qPCR reagent ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were incubated with a diluted primary antibody: rabbit polyclonal anti-DsRed at 1:1000 dilution (Takara Biomedical Technology ...
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bioRxiv - Neuroscience 2022Quote: ... and mouse anti-mCherry antibodies (Clontech; 1:2000) in PBST-azide with 3% NDS ...
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bioRxiv - Neuroscience 2022Quote: ... a mouse anti-mCherry primary antibody (Takara Bio, 1:5000) was used instead of the substance P primary antibody to visualize hM3Dq-mCherry or mCherry-expressing cells.
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bioRxiv - Neuroscience 2022Quote: ... they were placed under the binocular and 250 nl of pEGFP-C1 plasmid (Clontech) were injected through the median ocellus following the course of the ocellar tract ...
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bioRxiv - Neuroscience 2022Quote: ... were transformed with the plasmid peGFP-C1 (Clontech). Bacteria were grown overnight in lysogeny broth (LB ...
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bioRxiv - Neuroscience 2022Quote: ... pEGFP-N1 (Clontech), pCS-membrane-ceruleanFP (kind gift from Dr ...
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bioRxiv - Immunology 2022Quote: ... Shield-1 was obtained from Clontech and was used at a final concentration of 2.5 μM.
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bioRxiv - Immunology 2022Quote: Lytic cell death was determined by measuring LDH release from cell-free supernatants using a colorimetric assay (Promega or Takara) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... The g block and linearized plasmid backbone were ligated together using the In-fusion HD cloning kit (Takara Bio, catalog #638920).
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bioRxiv - Immunology 2022Quote: ... RetroNectin (TaKara, T202) was bound to a non-tissue culture treated plate overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... 50 ng of cDNA was used for quantification using SYBR Green mastermix (Takara) on Lightcycler 480 instrument (Roche) ...
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bioRxiv - Immunology 2022Quote: Cellular RNA samples were prepared using MN Nucleospin RNA kit (Takara). Equal quantities of RNA were reverse transcribed using Primescript Reverse transcriptase (Takara ...
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bioRxiv - Immunology 2022Quote: ... Equal quantities of RNA were reverse transcribed using Primescript Reverse transcriptase (Takara) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... and quantitative real-time PCR (q-PCR) was carried out using SYBR Premix Ex Taq (TaKaRa, Japan). Q-PCR was carried out in a Bio-Rad CFX96 system ...
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bioRxiv - Immunology 2022Quote: ... These RNA samples were reverse transcribed into cDNA using a PrimeScript RT reagent Kit (TaKaRa, Japan), and quantitative real-time PCR (q-PCR ...
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bioRxiv - Immunology 2022Quote: The total RNA of the cell samples was extracted with 1000-800 µl RNAiso Plus reagent (Takara, Kyoto, Japan) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... – PU6-pAzpa was co-transfected with plasmids expressing full-lengths mutant 2B4 TCR and CD3 subunits using Xfect transfection kit (TaKaRa). For incorporating pAzpa into TCR sites ...
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bioRxiv - Immunology 2022Quote: ... cDNA was synthesized using the SMARTer RACE cDNA Amplification kit (Clontech, Palo Alto, California, United States). Amplification of the TCRβ VDJ region was performed using previously described primers and amplification protocols(45) ...
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bioRxiv - Immunology 2022Quote: ... BAC DNA was purified using the NucleoBond BAC 100 kit (Takara). Viral DNA was isolated from viral stocks by digestion with proteinase K (Roche ...
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bioRxiv - Physiology 2022Quote: ... cDNA was synthesized using 2 μg RNA using the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa). Gene specific primers for quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Physiology 2022Quote: ... Viral titers were determined using the Adeno-X Rapid Titer Kit (Takara Bio, 632250).
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bioRxiv - Microbiology 2022Quote: ... The purified fiber was treated by adding proteinase K (PK) (Takara ST 0341) to 20 µL of sample (200 ng as estimated by dsDNA Qubit fluorometric quantification ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed using SYBR Premix Ex Taq (Takara). Roche LightCycler 480 thermal cycler was used to assess the melting curve and quantification of the PCR products ...
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bioRxiv - Microbiology 2022Quote: ... The PCR product was purified and ligated into the pET15b vector with a C-terminal His tag to create plasmid pET-AdpA by using the ClonExpress™ II One Step Cloning Kit (TaKaRa). The plasmid was transformed into E ...
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bioRxiv - Immunology 2022Quote: ... mouse anti-GFP clone JL-8 (Takara Bio Cat# 632380, RRID:AB_10013427), rabbit anti-FLAG clone D6W5B (Cell Signaling Technology Cat# 14793 ...
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bioRxiv - Microbiology 2022Quote: ... The PCR product and pET28b plasmid were digested with restriction endonuclease EcoRI and HindIII (Japanese Takara), then ligated with T4 DNA Ligase (Japanese Takara) ...
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bioRxiv - Immunology 2022Quote: ... Lactate dehydrogenase activity was quantified using the LDH Cytotoxicity Detection Kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... or MAP2 kinases under a bidirectional dox-inducible promoter were generated using lentiviral vector pInducer20bi-NA, a derivative of pInducer20-NA (Schmidt et al., 2016a) using the promoter from pTRE3G-BI (Takara Bio).
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bioRxiv - Immunology 2022Quote: ... Total RNA was then subjected to genomic DNA (gDNA) elimination and reverse-transcription according to PrimeScript™ RT reagent kit protocols (Takara). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... then ligated with T4 DNA Ligase (Japanese Takara), and transformed into Escherichia coli BL21 ...
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bioRxiv - Microbiology 2022Quote: ... and the PBSU101 plasmid was digested by BamHI and XbaI (Japanese Takara). Then PBSU101-pykF ...
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bioRxiv - Plant Biology 2022Quote: The lethal gene ccdB flanked by BsmBI sites was inserted into the positions 245 and 246 of Eggplant latent viroid (ELVd) accesion AJ536613.1 and cloned into a pBluescript SK plasmid using high-fidelity PrimeSTAR HS DNA Polymerase (Takara, Kusatsu, Japan). This plasmid linearized with BsmBI was used to ligate self-hybridized oligonucleotides into the positions 245 and 246 of ELVd ...
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bioRxiv - Plant Biology 2022Quote: ... The resulting cDNA was subjected to relative quantitative PCR using a SYBR Premix Ex TaqTM kit (TaKaRa) on a Roche LightCycler 480 real-time PCR machine ...
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bioRxiv - Plant Biology 2022Quote: ... . cDNA was synthesized by the use of PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) according to the reverse transcription protocol (Takara). The resulting cDNA was subjected to relative quantitative PCR using a SYBR Premix Ex TaqTM kit (TaKaRa ...
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bioRxiv - Plant Biology 2022Quote: ... the membranes were stripped for 15 minutes with Western BLoT Stripping Buffer (Takara) following the manufacturer’s instructions and reprobed with Ubiquitin11 (Agrisera AS08307 ...
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bioRxiv - Plant Biology 2022Quote: ... Growth was determined as described in the Yeast Two-Hybrid System User Manual (Clontech, Japan).
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bioRxiv - Plant Biology 2022Quote: ... Vectors were transformed into yeast strain Y2HGold (Clontech, Japan). Growth was determined as described in the Yeast Two-Hybrid System User Manual (Clontech ...
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bioRxiv - Plant Biology 2022Quote: ... and converted to cDNA using RNA to cDNA EcoDry™ Premix (Double Primed) reagents (Takara Bio USA). The cDNA was diluted 1:1 with water ...
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bioRxiv - Plant Biology 2022Quote: ... in-fusion cloning methods (Clontech/Takara) were employed to PCR-amplify and clone from S.viridis A10 genomic DNA a 4 kb fragment containing the SvRA1 transcribed region and regulatory sequences into pSB11-BAR as a HindIII-BamHI insertion ...
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bioRxiv - Plant Biology 2022Quote: ... and pMKMM21 (3.5 kb upstream SYP12A:5’UTR:miniTurbo- Myc-SYP12A:3’UTR) were generated by in-fusion cloning (HD enzyme mix; Takara Bio). Gateway binary vectors pMKMM22 and pMKMM23 for the expression of miniTurbo-Myc- MpSYP13B and miniTurbo-Myc-MpSYP12A were generated by LR-recombination (LR clonase ...
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bioRxiv - Plant Biology 2022Quote: ... and inserted into pSun using In-Fusion® cloning (Takara Bio Cat. #121416) according to manufacturers’ instructions.
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bioRxiv - Plant Biology 2022Quote: ... EGFP was inserted using HindIII and In-Fusion® cloning (Takara Bio Cat. #121416). For pAGO9::EGFP-AGO9 ...
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bioRxiv - Plant Biology 2022Quote: ... and EGFP was inserted using In-Fusion® cloning (Takara Bio Cat. #121416). For p35S::DAAO-GFP ...
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bioRxiv - Plant Biology 2022Quote: ... combined in tandem and integrated into the AscI site in pZH_OsU6sgRNA_SpCas-wt harboring SOG1-7D GT template by an In-Fusion reaction (Takara).