Labshake search
Citations for Takara Bio :
6801 - 6850 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... these four fragments were fused together using the In-Fusion system (Takara Bio, Inc., Japan).
-
bioRxiv - Immunology 2023Quote: Lenti-XTM 293T cells (Takara) were cultured at 0.5-1x106 cells/ml in Dulbecco’s modified Eagle’s Medium (DMEM ...
-
bioRxiv - Molecular Biology 2023Quote: ... pLKO.1-shFEN1 or pLKO.1 control vector was simultaneously transfected into LentiX293T (Cat# 632180 Clontech, Japan) with packaging plasmid ...
-
bioRxiv - Molecular Biology 2023Quote: ... or 100 ng of genomic DNA was amplified with Advantage GC 2 polymerase (Takara Bio 639114) using 1 M GC Melt and primers spanning the region from upstream of the splice donors to the 3’ end of the repeat ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Libraries construction (SMART-Seq v4 Ultralow input, Takara) with barcoded adaptors as well as RNA sequencing on NextSeq500-Illumina (single-end 1×5 bp ...
-
bioRxiv - Developmental Biology 2023Quote: ... First strand cDNA was prepared using the TAKARA PrimeScript RT Master Mix (TAKARA RR036A). Real-time PCR primers (Table S8 ...
-
bioRxiv - Cell Biology 2023Quote: ... The PISD-FLAG plasmid was generated by amplifying human PISD from cDNA and cloning it into the BglII and SalI sites of the pmCherry-N1 vector (Clontech). The C-terminal mCherry tag was subsequently replaced with 3xFLAG tag using the AgeI and NotI restriction sites ...
-
bioRxiv - Cancer Biology 2023Quote: ... Two-color FISH was conducted as previously described with minor adaptations.20,21 Undifferentiated ChiPSC22 were seeded on DEF-CS COAT-1-coated (Cellartis, Takara BioSciences) coverslips and differentiated ChiPSC22 on poly-L-lysine-coated coverslips ...
-
bioRxiv - Cancer Biology 2023Quote: Human DHRS7 was expressed as a GFP fusion using the pEGFP-N2 vector (Clontech) generated previously [5] ...
-
bioRxiv - Cancer Biology 2023Quote: ... was cultivated in the feeder-free DEF-CS system (Cellartis/Takara Bio Europe) under standard conditions ...
-
bioRxiv - Cancer Biology 2023Quote: ... 500 ng/mL doxycycline (Takara Bio) was added to the media and cells were incubated for 48 h at 37°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 0-500 ng/mL doxycycline (Takara Bio) and imaged in an Incucyte Live-Cell Analysis system (Sartorius ...
-
bioRxiv - Cancer Biology 2023Quote: ... concentrated 10x using LentiX concentrator reagent (Takara Bio 631231) and preserved in 0.4 ml aliquots at −80C.
-
bioRxiv - Cancer Biology 2023Quote: ... using CalPhos™ Mammalian Transfection Kit (Clontech, Inc., Mountain View, CA, USA), according to the manufacturer’s protocol as described (Wenta et al ...
-
bioRxiv - Cancer Biology 2023Quote: ... the final construct was assembled into the Adeno-X adenoviral system (Takara Bio) via In-Fusion cloning as described by manufacturer protocol.
-
bioRxiv - Cancer Biology 2023Quote: Sequencing libraries were generated by SMARTer Stranded Total RNAseq Kit – Pico Input Mammalian V3 (Takara Bio, #634485) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: Lentivirus was generated in a 10cm dish format by transfecting HEK293T Lenti-X cells (Takara) at roughly 80% confluency with 4.5μg of an equimolar mixture of three plasmids encoding the envelope and viral packaging components from the laboratory of Didier Trono (pMD2.G ...
-
bioRxiv - Molecular Biology 2023Quote: ... and cDNA was synthesized using PrimeScript RT reagent Kit (Perfect Real Time, TAKARA BIO Inc. Shiga, Japan). The mouse aorta was homogenized by TissueLyser II (Qiagen ...
-
bioRxiv - Biophysics 2023Quote: ... Pyroptosis was induced by addition of 500nM B/B-Homodimerizer (Takara Bio, AP20187) at 37°C and 5% CO2 ...
-
bioRxiv - Neuroscience 2023Quote: ... Fifty cells were collected in a tube containing ice-cold lysis buffer and processed for cDNA synthesis according to the Smart-Seq v4 manual (Takara Bio, 634890) with a PCR cycle number of 11 ...
-
bioRxiv - Physiology 2023Quote: ... Harvested conditioned media were incubated with Talon Metal Affinity resin (Clontech, Silicon Valley, CA, USA) at 4 °C overnight ...
-
bioRxiv - Plant Biology 2023Quote: ... thaliana (Takara Clontech Bio Europe, Cat.# 630487). For direct interaction studies co-transformation of bait and pray was employed ...
-
bioRxiv - Plant Biology 2023Quote: ... diluted to 1 µg/mL with Milli-Q ultra-pure water for 5 min at room temperature or with a pollen germination medium containing 2000-fold SYBR Green I (Cat#: 5760A, Takara) and 5 µg/mL DAPI (Cat# 10236276001 ...
-
bioRxiv - Neuroscience 2023Quote: ... Ds-red polyclonal anti-rabbit (1:200; Takara, 632496). C1q monoclonal anti-rabbit (1:1000 ...
-
bioRxiv - Neuroscience 2023Quote: ... using Infusion kit (Takara catalog # 638949). For some enhancers we instead cloned into a next generation reporter vector backbone that includes a SYFP2-P2A-3xFLAG-H2B reporter for detection of cytosolic SYFP2 and nuclear FLAG for simultaneous expression analysis and snRNA-seq ...
-
bioRxiv - Neuroscience 2023Quote: pEGFP-USP48 was generated by sub-cloning the mouse USP48 into the mammalian expression vector pEGFP-C2 (Clontech). pCMV-Myc-USP48 ...
-
bioRxiv - Neuroscience 2023Quote: ... A total of 14 PCR cycles of amplification was performed for each sample using PrimeSTAR GXL DNA Polymerase (Clontech, UK). Library preparation of the amplified cDNA products was then performed using SMRTbell Template Prep Kit v1.0 (PacBio ...
-
bioRxiv - Plant Biology 2023Quote: ... The FW2.2 full-length coding sequence was amplified from cDNAs prepared from tomato (cv. AC) fruits at 5 DPA using PrimeSTAR MAX DNA polymerase (TAKARA BIO Inc., Kusatsu, Japan) and primers including the attB sites (Supplemental Table 1) ...
-
bioRxiv - Plant Biology 2023Quote: ... All of these fragments were cloned into the KpnI site in the pZH2Bik vector using In-Fusion cloning (Takara) so as to be driven by the rice ubiquitin promoter ...
-
bioRxiv - Genetics 2023Quote: ... and cloned into pMSCV-Thy-IRES 1.1 vector (Clontech) using EcoRI and ClaI restriction sites.
-
bioRxiv - Genetics 2023Quote: ... One-third of the total media volume of Lenti-X Concentrator (631232, Takara Bio, Shiga, Japan) was added to the viral media and refrigerated for 30 minutes at 4°C before being centrifuged for 45 minutes at 1500g and 4°C ...
-
bioRxiv - Synthetic Biology 2023Quote: ... qPCR was performed using TB Green Premix Ex Taq (Takara, Beijing, China). Primers used in qPCR are listed in Supplementary Table S1 ...
-
bioRxiv - Molecular Biology 2023Quote: ... the open reading frames (ORFs) of genes and their mutant alleles were amplified and cloned into the pGADT7 prey or pGBKT7 bait vector (TaKaRa Bio, Tokyo, Japan). The resulting bait and prey constructs were transformed in pairs into yeast strain AH109 and assayed for growth on SD-His-Leu-Trp plates ...
-
bioRxiv - Neuroscience 2023Quote: ... RNA was then frozen and sent to Novogene for ultra-low-input bulk RNA sequencing or converted into cDNA with the PrimeScript RT Master Mix (Takara Bio, RR036A) for quantification of mRNA level using qPCR4 ...
-
bioRxiv - Microbiology 2023Quote: ... 0.8 U/μl recombinant RNase inhibitor (Takara, Cat# 2313B)] and incubated at room temperature for 10 m ...
-
bioRxiv - Molecular Biology 2023Quote: ... and the NucleoSpin RNA Virus was from Takara (cat. 740956)
-
bioRxiv - Molecular Biology 2023Quote: ... 12.5 U/μl Smartscribe reverse transcriptase (639538, Takara), 2.5 mM dNTPs ...
-
bioRxiv - Molecular Biology 2023Quote: ... we enriched the lentiviral particles with Lent-X Concentrator (Cat# 631231, Clontech, Japan) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: Lentivirus was produced using a standard protocol64 from a 15 cm plate of 25M lenti-x 293T cells (Clontech #632180) via polyethylenimine (PEI ...
-
bioRxiv - Molecular Biology 2023Quote: ... The RNA was then reverse-transcribed into complementary DNA (cDNA) using the RR036A PrimeScript™ RT Reagent Kit (Perfect Real Time) supplied by TaKaRa. The amplification products were quantified using SYBR Green (Vazyme) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Relative titer was estimated by Lenti-X GoStix Plus (Takara #631280) and was similar for all viral constructs.
-
bioRxiv - Immunology 2023Quote: ... The virus titer was determined using Lenti-XTM GoStix Plus (Clontech).
-
bioRxiv - Cell Biology 2023Quote: ... gDNA was amplified using CloneAmp™ HiFi PCR Premix (Takara 639298) and dmd Sanger sequencing primers ...
-
Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... using in-fusion cloning kit (Takara, Kusatsu, Japan). To generate pFA6a-link-GBP-CaURA3 ...
-
bioRxiv - Cell Biology 2023Quote: ... Quantitative RT-PCR was performed using a Thermal Cycler Dice Real Time System II (Takara Bio Inc., Otsu, Japan) and SYBR Premix Ex Taq II (Takara ...
-
bioRxiv - Plant Biology 2023Quote: ... cDNA from a microgram of RNA was obtained using a PrimeScript RT reagent kit (Perfect Real Time, Takara Bio Inc., Otsu, Shiga, Japan) following its instructions ...
-
bioRxiv - Cell Biology 2023Quote: The following plasmids were commercially available or described previously: pEGFPC1 (Clontech), β-actin-GFP and β-actin-tdTomato [54] ...
-
bioRxiv - Immunology 2023Quote: ... cDNA was generated for each sample using the SMART-Seq v4 kit (Takara). Samples were end-repaired ...
-
bioRxiv - Molecular Biology 2023Quote: ... APEX2 and p110 were cloned into pLVX-IRES-puro (Takara, 632183) via a series of restriction enzyme digests and ligations ...
-
bioRxiv - Neuroscience 2023Quote: ... neurons were transfected with a pEGFP-N2 plasmid from Clontech. Prior to incubation ...