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Citations for Takara Bio :
6751 - 6800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The pTRE3G vector containing PFK1-mEGFP was then co-transfected into HeLa Tet-On 3G cells (Clontech) along with a linear selection marker of hygromycin using Xfect (Clontech) ...
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bioRxiv - Biochemistry 2022Quote: ... wild type and mutant constructs of full-length PKD1 were cloned into the EGFP-C1 and mCherry-C1 vectors (Clontech), resulting in N-terminally labelled fusion proteins ...
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bioRxiv - Biochemistry 2022Quote: ... Soluble fraction was purified using Talon affinity resin (Takara). The resin was equilibrated with buffer A (20mM Tris-HCl pH 8 ...
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bioRxiv - Biochemistry 2022Quote: ... NUT3 and NUT4 and RFP tagged p300 were cloned into pEGFP-C1 vector (Clontech), HA-tagged p300 constructs (WT ...
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bioRxiv - Biochemistry 2022Quote: ... cDNA synthesis was carried out using the Primescript™ RT reagent kit with gDNA eraser (perfect real time) (TaKaRa, JP) following the manufacturer’s instructions.
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bioRxiv - Biochemistry 2022Quote: ... 9, 11, 13, 20, or 45 (SERF2, C9orf16, C19orf53, C11orf58, BEX3, or SERBP1 respectively) was inserted into pCold I (Takara), together with the client protein ...
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bioRxiv - Biochemistry 2022Quote: ... respectively) were carried out using a growth medium containing 10% Tet-Approved FBS (Clontech) in place of standard growth serum ...
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bioRxiv - Cell Biology 2022Quote: ... was obtained from Takara (Saint-Germain-en-Laye, France). IR dye conjugated secondary antibodies were used at a dilution of 1:20,000 and purchased from LI-COR Biosciences (Cambridge ...
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bioRxiv - Developmental Biology 2022Quote: ... the assembled targeting vector was mutated using site-directed mutagenesis using In-Fusion HD Cloning Plus (Takara, 638909). NCRM1 iPSCs were transfected with both Guide RNA vector (PSpCas9 (BB)-2A-GFP ...
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bioRxiv - Biochemistry 2022Quote: ... Normalization was made by measuring the β-Gal activity after 1-hour incubation of 7.5 µl of cellular extract with 50 µl of β-Gal substrate (β-Gal detection kit II, Clontech).
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bioRxiv - Biochemistry 2022Quote: ... Diluted lysate was incubated with 1.25 ml His60 Ni Superflow affinity resin (Takara) per liter of expression culture ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant His-tagged protein was purified from cell-free extracts using immobilised metal affinity chromatography (IMAC using Talon resin; Clontech) as described previously (34) ...
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bioRxiv - Biochemistry 2022Quote: ... cells were transfected with plasmids expressing either SARS-COV-2 S protein WT or mutants (E1182K, L1193G, E1182K/L1193G, L1182K/L1186G/L1193G) by using Calcium phosphate transfection kit (Takara-Bio). 24 h post transfection ...
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bioRxiv - Biochemistry 2022Quote: ... pro-meprin α was activated by trypsin cleavage applying immobilized trypsin on magnetic beads (PT3957-1, Takara, buffer 30 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... The dialyzed fraction was mixed for 3 h with Talon Metal Affinity Resin (Clontech) that had been equilibrated with Buffer T ...
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bioRxiv - Biochemistry 2022Quote: ... the supernatant was nutated with 1 mL TALON resin (Takara) for 30 min at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... one microgram of total RNA was employed to obtain the corresponding cDNA target sequences using an oligo(dT)18 primer and the PrimeScript RT reagent kit (Perfect Real Time, Takara Bio Inc., Otsu, Shiga, Japan), following the manufacturer’s directions ...
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bioRxiv - Biochemistry 2022Quote: ... 1-206) and SYCE3 (1-88) were cloned into pGBKT7 vectors (Clontech) and human sequences for SYCP1 (1-811) ...
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bioRxiv - Biochemistry 2022Quote: ... TEX12 (1-123) and SIX6OS1 (1-587) were cloned into pGADT7 vectors (Clontech). The Matchmaker™ Gold system (Clontech ...
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bioRxiv - Biochemistry 2022Quote: ... The Matchmaker™ Gold system (Clontech) was used for Y2H analysis ...
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bioRxiv - Bioengineering 2022Quote: ... 800 μL of dNTPs (Takara), 50 μL of P5 stagger primer mix (stock at 100 μM concentration) ...
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bioRxiv - Bioengineering 2022Quote: HEK293T cells were transfected with given plasmids and then harvested for RNA extraction by NucleoSpin RNA Plus Kit (Takara) 48 hour after transfection ...
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bioRxiv - Bioengineering 2022Quote: ... Virus-containing supernatants were concentrated with Lenti-X and Retro-X concentrator (Takara), respectively ...
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bioRxiv - Bioengineering 2022Quote: ... Bead-stimulated cells were transferred to Retronectin-coated (Takara) virus-containing plates and incubated overnight ...
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bioRxiv - Biochemistry 2022Quote: ... IC3 was purified by affinity chromatography (TALON resin, Clontech) using the two N-terminal histidine tags of aIF5B and aIF1A ...
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bioRxiv - Biochemistry 2022Quote: ... Each 50 µL reaction consisted of 25 µL of PrimeSTAR® Max DNA Polymerase (Takara, Japan), 21 µL of RNase-free water ...
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bioRxiv - Immunology 2022Quote: ... TB green Advantage qPCR Premix (Takara) was used ...
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bioRxiv - Molecular Biology 2022Quote: ... digested pHCMV backbones using the InFusion cloning kit (Takara Bio, 638920). To generate siRNA-resistant SUPYN rescue constructs ...
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bioRxiv - Genetics 2022Quote: ... 0.375 µL of ExTaq polymerase (Takara Bio, Mountain View, CA, USA), 5 µL of ExTaq buffer ...
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bioRxiv - Cell Biology 2022Quote: ... in the case of homodimers and Streptactin-XT resin and TALON resin (Takara Bio Inc., Kusatsu, Japan) in the case of heterodimers ...
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bioRxiv - Microbiology 2022Quote: ... Membrane was immunoblotted for ≥ 3 h with primary monoclonal anti-GFP (1:5,000) antibodies (JL8, Clontech-Takara), then followed by immunoblotting for ≤ 1 h with secondary antibodies ...
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bioRxiv - Microbiology 2022Quote: ... Membrane was immunoblotted for ≥ 3 h with primary monoclonal anti-GFP (1:5,000) antibodies (JL8, Clontech-Takara), then followed by immunoblotting for ≤ 1 h with secondary antibodies ...
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bioRxiv - Plant Biology 2022Quote: The coding region of each P450 gene was cloned into pCAMBIA1390 vector with the In-Fusion DH Cloning Kit (TaKaRa, Kusatsu, Japan) or SLiCE reaction (Motohashi ...
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bioRxiv - Plant Biology 2022Quote: ... phyllopogon (line 511) using primers listed in Table S1 with KOD FX Neo (TOYOBO) or PrimeSTAR GXL DNA Polymerase (Takara). The amplicons were subcloned using pGEM-T Easy Vector Systems Kit (Promega ...
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bioRxiv - Immunology 2022Quote: ... Viruses were packaged in human embryonic kidney (HEK) 293 cells and viral supernatants were processed using Retro-X concentrators (Takara Bio. USA Inc). Naïve CD8 T cells were purified by negative-selection and stimulated in vitro with plate-bound anti- CD3/CD28 ...
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bioRxiv - Immunology 2022Quote: ... Vero E6-TMPRSS258 and Lenti-X 293T cells (Takara) were cultured in Dulbecco’s Modified Eagle’s medium (DMEM) ...
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bioRxiv - Molecular Biology 2022Quote: ... The TOP1MT open reading frame (ORF) was cloned using InFusion cloning (Takara, Clonetech) into the pRV100G retroviral vector containing a C-terminal T2A mCherry tag ...
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bioRxiv - Molecular Biology 2022Quote: ... Long range PCR reactions were performed using the Takara LA Taq polymerase (Takara Bio, RR002M), with 250 ng genomic DNA ...
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bioRxiv - Immunology 2022Quote: ... and mRFP (Takara Bio), were either transfected into B16-F10 cells using the calcium chloride precipitation method (all FPs except ZsGreen) ...
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bioRxiv - Developmental Biology 2022Quote: Trophectoderm biopsies containing 5-10 cells from blastocyst-stage embryos (n=24) were processed for RNA-seq using a commercial kit (Takara Bio ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was then reverse transcribed using a Primescript RT reagent kit (Takara Bio, #RR037A) with random hexamers ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was reverse transcribed using the Primescript RT reagent kit (Takara Bio, #RR037A) with random hexamers ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were harvested by centrifugation and cell pellets were lysed through xTractor Lysis Buffer (Takara, Japan) supplemented with 20 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... and the linearized plasmid gel-extracted and assembled with PCR-amplified codon-optimized mNeongreen or mScarlet using In-fusion cloning (Takara). To generate pPD95.75-itr- 1p-mNG or pPD95.75-itr-1p-mSc plasmids pPD95.75-mNG plasmid was digested with HindIII and PstI ...
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bioRxiv - Developmental Biology 2022Quote: ... 2 mM DTT (Takara Bio, #639537), 1 mM dNTPs (Takara Bio ...
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bioRxiv - Biochemistry 2022Quote: ... Positive yeast transformants were selected on minimal synthetic defined (SD) base (Takara) media supplemented with complete supplement mixture without uracil (−Ura ...
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bioRxiv - Developmental Biology 2022Quote: ... Complementary DNA (cDNA) was prepared using the 5X Prime Script RT Master Mix (Takara RR-036A-1) with 1 μg of total RNA ...
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bioRxiv - Biochemistry 2022Quote: ... roughly 5 million AAV pro293T cells (Takara, San Jose, CA) in 30 mL of media were seeded overnight in a T175 flask (Greiner Bio-One ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by ligating these fragments using an In-Fusion system (TAKARA). Hevin-N11 fragment was inserted into pCR-blunt using In-Fusion system ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 μl of Ligation Mix (5 mM ATP, 7U Terminal Deoxynucleotidyl Transferase (TdT) (2230B, Takara), 15 U T4 RNA Ligase High Concentration (M0437 ...