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Citations for Takara Bio :
6651 - 6700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... were reverse transcribed into cDNA in a volume of 10 µl using the PrimeScript RT Master Mix (Perfect Real Time, TaKaRa) (37°C ...
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bioRxiv - Cell Biology 2022Quote: Cells cultured in either 12- or 24-well plates were washed twice with cold PBS and harvested using a TaKaRa MiniBEST universal RNA extraction kit (Takara, Japan). RNA was purified using the same kit according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... 100 ng of polyA selected RNA from the human lung carcinoma cell line A549 (Takara #636141) was used as input ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.25 ng to 10 ng of total RNA was used for cDNA library preparation using a kit suitable for RNA isolation at pico-molar concentrations (MARTer Stranded Total RNA-Seq Kit v2-Pico Input Mammalian) following manufacturer recommended protocol (Clontech/Takara Bio #635005). Sequencing was performed by the UM DNA Sequencing Core ...
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bioRxiv - Biochemistry 2022Quote: ... coding regions were cloned downstream of the CMV promoter in derivatives of the pEGFP-N1 plasmid (Clontech) with an NLS and linker sequences to EGFP or mCherry (Supplemental Table 2) ...
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bioRxiv - Biochemistry 2022Quote: ... was added at the N-termini of EGFP and mCherry (Clontech, Mountain View, CA). For intramolecular FRET experiments ...
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bioRxiv - Microbiology 2022Quote: ... The 5’ end regions of MIC14 and MIC15 were amplified using the SMART RACE cDNA Amplification Kit (Clontech BD) using total or poly(A)+tachyzoite RNA (strain RH ...
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bioRxiv - Cell Biology 2022Quote: Mouse Itgb3 was cloned into the pLVX-EF1α-IRES-Puro vector (Takara Bio) per a previously described cloning strategy (Dave et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Flt3 ligand (50 ng/ml) and IL-3 (20 ng/ml) onto plates coated with retronectin (Takara Bio).
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bioRxiv - Cell Biology 2022Quote: ... Lentiviral supernatants were collected 48 h post-transfection and concentrated by adding one volume of Lenti-X Concentrator (Takara Bio) to three volumes of lentivirus-containing supernatant and incubating at 4°C overnight ...
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bioRxiv - Cell Biology 2022Quote: Lenti-X Expression System (Takara Bio) was utilized to generate lentivirus as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Nup98 was amplified from a HeLa cDNA library from Clontech/Takara ...
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bioRxiv - Microbiology 2022Quote: ... the 5′-terminus of the viral genome was determined by 5′/3′ RACE kits (TaKaRa). The resulting whole genome sequence of the GX_P2V variant was deposited in GenBank (accession number MW532698) ...
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bioRxiv - Neuroscience 2022Quote: ... EGFP-positive cells from control and Yap1-5SA groups were directly collected into ice-cold lysis buffer (0.2 %Triton X-100, containing RNAse inhibitors, Clontech, cat# 635013) in 96-well plates (Eppendorf ...
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bioRxiv - Immunology 2022Quote: ... we expressed shRNA using SIREN-RetroQ (Clontech) gammaretroviral vector containing shRNA sequence targeting human TRIM5(Stremlau et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... rat anti-E-Cadherin (TAKARA; M110), rat anti-Endomucin (Santa Cruz ...
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bioRxiv - Cell Biology 2022Quote: ... All plasmids during this study were made with In-Fusion HD Cloning Plus (Takara, 638909). All yeast strains are isogenic to BY4741 ...
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bioRxiv - Cell Biology 2022Quote: ... the medium was exchanged with primate ES Cell Medium containing doxycycline (TaKaRa) (1.0 μg/mL) ...
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bioRxiv - Microbiology 2022Quote: ... Lentiviral titer was determined by using the Lenti-X p24 Rapid Titer Kit (Takara). Then ...
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bioRxiv - Microbiology 2022Quote: ... The human kidney epithelial cell line Lenti-X™ 293T (Takara) was cultivated in DMEM supplemented with 10 % (v/v ...
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bioRxiv - Microbiology 2022Quote: ... Two PCR products were inserted into the vector backbone using In-Fusion HD Cloning Kit (Takara Bio) to generate pLJM1-LTR-FT ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 Beta RBD was purified using a 5 mL HisTalon Superflow cartridge (Takara Bio) followed by buffer exchange into PBS using a HiPrep 26/10 desalting column (Cytiva).
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bioRxiv - Immunology 2022Quote: ... using SYBR Premix Ex Taq II mix (TaKaRa Bio) as described [8] ...
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bioRxiv - Immunology 2022Quote: ... Bulk TCR sequencing was performed using the SMARTer® Mouse TCR a/b Profiling Kit (Takara Bio). Libraries were sequenced using the 600-cycle MiSeq Reagent Kit v3 (Illumina ...
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bioRxiv - Evolutionary Biology 2022Quote: The homogenate of the symbiotic organ was subjected to total RNA extraction using RNAiso (Takara Bio, Japan) and RNeasy Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... The viral RNA copy number was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). Fluorescent signals were acquired using QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using the One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...
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bioRxiv - Microbiology 2022Quote: ... the CPER products were transfected into HEK293-C34 cells using TransIT-LT1 (Takara, Cat# MIR2300) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: DNA assemblies were conducted with the In-Fusion HD Cloning Kit (Takara BIO) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Pyridylamination of N-glycans was performed using a pyridylamination manual kit (Takara Bio Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-STEM101 antibody (Takara Bio Inc., 1:100) to stain for transplanted human NPCs ...
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bioRxiv - Neuroscience 2022Quote: ... CquiOR27 and CquiOR28 were subcloned separately from pGEM-T-CquiOR27 and pGEM-T-CquiOR28 into pGEMHE using In-Fusion HD Cloning Kit according to the manufacturer’s instructions (Clontech). PCR In-Fusion primers were designed according to the user manual ...
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bioRxiv - Neuroscience 2022Quote: ... Mover-IRES-palmGFP (promoter: CMV; backbone: Clontech N1); mGFP-Mover (promoter ...
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bioRxiv - Neuroscience 2022Quote: ... Antennal cDNA was synthesized from 1 μg of antennal total RNA using SMARTer™ RACE cDNA amplification kit according to manufacturer’s instructions (Clontech, Mountain View, CA). To obtain full-length coding sequences ...
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bioRxiv - Molecular Biology 2022Quote: ... and purified by MPG streptavidin beads (Takara, cat. 6124A) followed by RNAse (Promega ...
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bioRxiv - Molecular Biology 2022Quote: ... gDNA extracted and PCR genotyped with primers (Supplemental table 1) flanking each homologous arms using PrimeStar GXL DNA polymerase (Takara). Correctly targeted clones were further expanded and banked ...
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bioRxiv - Molecular Biology 2022Quote: ... pseudotuberculosis were performed using an In-Fusion HD cloning kit (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The virus-containing media were harvested and concentrated with Lenti-X concentrator (Clontech) per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Bead-stimulated cells were transferred to Retronectin-coated (Takara) virus-containing plates and incubated overnight ...
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bioRxiv - Bioengineering 2022Quote: ... Virus-containing supernatants were concentrated with Retro-X concentrator (Takara). PBMCs were stimulated for 2 days with CD3/CD28 T cell activation beads (Thermo # 11131D ...
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bioRxiv - Immunology 2022Quote: ... The SMART-Seq v4 Ultra Low Input RNA Kit (TaKaRa) was used to generated amplified cDNA ...
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bioRxiv - Molecular Biology 2022Quote: ... pLVX-Tet-One-Puro vector (Clontech, #631847), encoding for an engineered Tet activator and the corresponding inducible promoter ...
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bioRxiv - Synthetic Biology 2022Quote: ... the clarified lysate was bound to TALON® Metal Affinity Resin (Takara Bio) for 1 h at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... The DNA purification was conducted using NucleoBond® HMW DNA kit (from TaKaRa) and the purification steps were followed exactly as described in the protocol accompanying the kit ...
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bioRxiv - Genetics 2022Quote: ... 0.375 µL of ExTaq polymerase (Takara), 5 µL of ExTaq buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... RNAs bound by miR-2861 were purified with Trizol reagent (Takara, Shiga, Japan), and assessed through qRT-PCR.
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bioRxiv - Cancer Biology 2022Quote: SV40-MES-13 cells were lysed with xTractor Buffer Kit (CLONTECH Laboratories, Inc., CA, USA). Protein specimens were quantified through BCA method and electrophoresed through 10% SDS-PAGE gels ...
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bioRxiv - Cancer Biology 2022Quote: ... using T4 DNA ligase (Takara Bio). The ligation mixes were transformed into Stbl3 competent cells and the resultant clones were screened by checking the insert sizes and direct sequencing ...
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bioRxiv - Biophysics 2022Quote: ... and slowly shaken with all-trans-retinal (added to a final concentration of 25 μM) in the dark at room temperature for 3–4 h in the presence of 0.5 % of Westase (Takara Bio, Inc.) to digest the cell wall ...
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bioRxiv - Plant Biology 2022Quote: ... and NotI (Takara Bio) and then ligated to generate pGEX6P-1_MpIAA and pGEX6P-1_MpIAAmutDII ...