Labshake search
Citations for Takara Bio :
6601 - 6650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2022Quote: Experiments were performed using PtK2 GFP-α-tubulin cells (stable line expressing human α-tubulin in pEGFP-C1, Clontech Laboratories ...
-
bioRxiv - Biophysics 2022Quote: ... This was then loaded onto a column with 5 ml His60 Ni-Superflow Resin (Clontech) previously equilibrated in the lysis buffer ...
-
bioRxiv - Biochemistry 2022Quote: ... aIF1A was purified as described (24) except that a standard affinity chromatography step on Talon resin (Clontech) was added to the original protocol ...
-
bioRxiv - Biochemistry 2022Quote: ... The precipitated material was removed by centrifugation and the soluble fraction was loaded onto a column (5 mL) containing Talon affinity resin (Clontech) equilibrated in buffer A ...
-
bioRxiv - Biophysics 2022Quote: Mouse anti-GFP mAb (catalog number 632381) was obtained from Clontech (San Jose, CA, USA). Mouse anti-mCherry mAb (catalog number NBP1-96752 ...
-
bioRxiv - Molecular Biology 2022Quote: ... was based on a pIRES2-EGFP vector (Clontech, Mountain View, CA; #6029-1). cDNA inserts from coding domain sequences (CDS ...
-
bioRxiv - Microbiology 2022Quote: ... and T4 (C) were determined by digerstion with QuickCut™ EcoR V (Takara), MspJI (NEB) ...
-
bioRxiv - Immunology 2022Quote: Total RNA was prepared by using RNAiso Plus (Takara). 1 μg of total RNA was used to synthesize cDNA with PrimeScript RT reagent kit (RR047A ...
-
bioRxiv - Immunology 2022Quote: ... 1 μg of total RNA was used to synthesize cDNA with PrimeScript RT reagent kit (RR047A, Takara) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... total RNA was prepared with RNAiso Plus (Takara). 1μg EU-labeled RNA was biotinylated with 0.5 mM biotin azide in Click-iT reaction buffer for 30min ...
-
bioRxiv - Neuroscience 2022Quote: ... TagBFP-P2A-Pre-Sphere-SF-iGluSnFR and mCherry-P2A-Post-Sphere-SF-iGluSnFR sequences were inserted into the pAAV-hSyn-EGFP Vector by replacing the EGFP using Infusion (Takara Bio). AAV serotype 2 was amplified in HEK293T cells by transfecting pAAV.hSynapsin.TagBFP-P2A-Pre-Sphere-SF-iGluSnFR or pAAV.hSynapsin.mCherry-P2A-Post-Sphere-SF-iGluSnFR via the pRC2-mi342 and pHelper vectors ...
-
bioRxiv - Neuroscience 2022Quote: ... rabbit anti-Dsred (1:200)(Takara), mouse anti-Lacz (1:200)(Promega) ...
-
bioRxiv - Neuroscience 2022Quote: ... Shortly, hiPSC lines (C1, C2, C3) and the commercial human iPSC-line Chipsc4 (Takara, Sweden) were cultured and differentiated into cortical NSCs as described elsewhere (23) ...
-
bioRxiv - Neuroscience 2022Quote: AAVs were packaged via calcium phosphate transfection of HEK293 cells as detailed in (McClure et al 2011) and purified using an AAVpro purification kit (Takara Bio).
-
bioRxiv - Neuroscience 2022Quote: ... coli cells induced with 100 mM IPTG and purified from bacterial extracts using TALON beads (Takara Bio, Catalog # 635502) according to the vendor’s instructions.
-
bioRxiv - Developmental Biology 2022Quote: ... and reverse transcription was performed to generate cDNA using the PrimeScript RT Master Mix (RR036; Takara, Kusatsu, Japan). The RNase-Free DNase Set (79254 ...
-
bioRxiv - Developmental Biology 2022Quote: ... An aliquot of 1 μg of the total RNA was used to synthesize cDNA using PrimeScript™ RT reagent Kit with gDNA eraser (Takara). qRT-PCR analysis was performed on a StepOnePlus Real-Time PCR system (Applied Biosystems ...
-
KIF24 controls the clustering of supernumerary centrosomes in pancreatic ductal adenocarcinoma cellsbioRxiv - Cell Biology 2022Quote: ... The virus supernatant was harvested 72 hrs post-transfection and concentrated using Lenti-X Concentrator (Clontech). Panc1 ...
-
bioRxiv - Cancer Biology 2022Quote: ... 5 million cells were electroporated with 5 µg of plasmids encoding tested lncRNAs or with of the pEGFP-N3 reporter vector (Clontech) as previously described 30 ...
-
bioRxiv - Cancer Biology 2022Quote: ... 10 ml of virus containing media were concentrated with Lenti-X concentrator (Takara Bio, 631231) to a titer sufficient to decrease pStat1 levels by 25% (Mean pStat1+ CD11b+ cells ...
-
bioRxiv - Cell Biology 2022Quote: Naïve human PSCs were cultured in the PXGL medium: Ndiff227 (Takara Bio) medium supplemented with PD0325901 (Sigma,1 μM) ...
-
bioRxiv - Cancer Biology 2022Quote: ... with the SYBR Premix Ex Taq (Takara). The primers used for each reaction are indicated in Supplementary Table S1 ...
-
bioRxiv - Cancer Biology 2022Quote: ... using the InFusion ligation kit (Takara Bio). The cloned vector was sequenced to verify appropriate insertion of the shRNA ...
-
bioRxiv - Cancer Biology 2022Quote: ... Lenti-X 293T cells were obtained from Clontech, and maintained in DMEM media (Hyclone or Gibco ...
-
bioRxiv - Biochemistry 2022Quote: ... Human DOCK10 DNA was synthesized by Clontech (NM_014689). The DHR2 domain of human DOCK11 DNA (NM_144658.3 ...
-
bioRxiv - Biochemistry 2022Quote: ... AAV-GFP and AAV-Mafa were purified with the AAV pro Extraction Solution Kit (6235, Takara, Shiga, Japan), and the titers were determined with a Quick Titer AAV Quantification Kit (VPK-145 ...
-
bioRxiv - Biochemistry 2022Quote: ... 53) and Hif1β shRNA (target sequence: 5’-GGACAGAGATCCAAGGTTT-3’) were cloned into the pSIREN-RetroQ expression vector (631526; Clontech Laboratories Inc., Mountain View, CA). The FLAG-tagged mouse Pdx1 coding sequence was amplified by PCR and subcloned into a pMXs-Neo Retroviral vector (RTV-011 ...
-
bioRxiv - Molecular Biology 2022Quote: ... with SYBR Green qPCR Master Mix (TaKaRa Bio). Relative gene expression levels were determined via 2-ΔΔCt analysis ...
-
bioRxiv - Molecular Biology 2022Quote: ... EBIV M/L segment-specific sequences were amplified by PCR including Universal Primer A Mix (Takara), Gene-Specific Primers (GSPs ...
-
bioRxiv - Molecular Biology 2022Quote: ... 2.5 µl cDNA and 1.25 U SeqAmp™ DNA polymerase (Takara). The PCR products were separated in a 1% agarose gel and recovered with a Gel extraction kit (Omega) ...
-
bioRxiv - Molecular Biology 2022Quote: ... A 25 nt poly(A) sequence was inserted after the 3’ UTR using the In-Fusion (Takara Bio) method.
-
bioRxiv - Molecular Biology 2022Quote: ... They were ligated using In-fusion (Takara Bio Inc, Shiga, Japan) and the resultant plasmid was named psfRps29-GST ...
-
bioRxiv - Molecular Biology 2022Quote: The Delta PAS sfRps29 vector was generated by PCR using PrimeSTAR MAX Polymerase (Takara Bio) with primers containing the point mutation (forward ...
-
bioRxiv - Molecular Biology 2022Quote: ... and Tet System approved FBS (631106, Takara) containing medium was used to culture Dox (A2951 ...
-
bioRxiv - Molecular Biology 2022Quote: ... RNA was isolated using the TRIzol method and the expression of miRNAs analyzed by qRT-PCR with the Mir-X miRNA qRT-PCR TB Green kit (Takara Bio Inc ...
-
bioRxiv - Molecular Biology 2022Quote: ... These sequences were obtained from pPIGA3GFP (inverted repeats of piggyBac transposon [17]) and pAcGFP-Hyg C1 vector (CMVp-AcGFP and SV40p-Hyg) (632492; Takara Bio USA). Recombinant baculovirus was then produced using a Bac-to-Bac system ...
-
bioRxiv - Cancer Biology 2022Quote: ... RNA was reverse-transcribed using the PrimeScript RT reagent Kit (TaKaRa). Transcript expressions were determined by qRT-PCR using SYBR Premix Ex Taq II (TaKaRa ...
-
bioRxiv - Cancer Biology 2022Quote: ... Transcript expressions were determined by qRT-PCR using SYBR Premix Ex Taq II (TaKaRa) and the QuantStudioTM real-time PCR instrument (Applied Biosystems) ...
-
bioRxiv - Cancer Biology 2022Quote: ... total RNA was isolated from liver of mice using RNAiso plus (TaKaRa). RNA was reverse-transcribed using the PrimeScript RT reagent Kit (TaKaRa) ...
-
bioRxiv - Neuroscience 2022Quote: ... Reverse transcription was performed using PrimeScript II 1st strand cDNA Synthesis Kit (Takara, Japan) following the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2022Quote: The following plasmids were used: GFP and mCherry (Clontech/Takara Bio); GFP-fascin WT/S39A/S39D (Zanet et al. ...
-
bioRxiv - Cancer Biology 2022Quote: ... The SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian (Clontech Laboratories, Inc., A Takara Bio Company,California, USA) was used in the succeeding steps ...
-
bioRxiv - Cancer Biology 2022Quote: ... The SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian (Clontech Laboratories ...
-
Antigen-dependent inducible T cell reporter system for PET imaging of breast cancer and glioblastomabioRxiv - Synthetic Biology 2022Quote: ... HSV-tkSR39-T2A-sIL2 construct was cloned from HSV-tkSR39-GFP using In-Fusion cloning (Takara Bio, CA) after adding six C-terminal amino acids (EMGEAN ...
-
bioRxiv - Cell Biology 2022Quote: ... and ligating the products into a digested pEGFP-N2 vector (Clontech) (pAT4206) ...
-
bioRxiv - Bioengineering 2022Quote: ... A Lenti-X concentrator (Takara Bio USA) was used according to the manufacturer’s protocol to concentrate the virus 10-20× and the resulting lentiviral stocks were aliquoted and stored at −80°C ...
-
bioRxiv - Cancer Biology 2022Quote: ... transduced to express CD19 CAR construct (FMC63 or CAT) with 1 ml of LV supernatant in RetroNectin®(Takara Bio, Kusatsu, Shiga, Japan)-coated 24-well plates and spinoculated at 1,000 g for 40 min at room temperature ...
-
bioRxiv - Developmental Biology 2022Quote: ... Reverse transcription was performed using PrimeScript™ II Reverse Transcriptase (Takara). The quantitative RT-PCR analysis was performed by StepOneTM (ThermoFisher ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... and glyceraldehyde 3-phosphate dehydrogenase (5′-GCACCGTCAAGGCTGAGAAC-3′ and 5′-TGGTGAAGACGCCAGTGGA-3′; Takara Bio Inc., Shiga, Japan) were used for RT-PCR.
-
bioRxiv - Neuroscience 2022Quote: ... Indexed libraries were generated using the SMARTer smRNA-Seq kit for Illumina (Takara).