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Citations for Takara Bio :
6551 - 6600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... Transcriptional levels of genes were determined using Premix Ex Taq (Takara) and analysed with a Quantstudio 6 Flex system ...
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bioRxiv - Developmental Biology 2022Quote: ... 250–2000 pg of total RNA was loaded as a template for cDNA synthesis using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech) with template switching technology ...
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bioRxiv - Developmental Biology 2022Quote: ... https://www.addgene.org/112849/)10 between the EcoRI and SpeI restriction sites by multi-cassette assembly using the InFusion cloning kit (Takara Inc.). The correct sequence of the donor region was validated by Sanger sequencing.
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bioRxiv - Developmental Biology 2022Quote: ... PCR templates were generated by PCR reaction with biotinylated primers using high fidelity ClonAmp HiFi PCR mix (Takara Inc.). A mixture of Cas9-mSA mRNA (75ng/ml) ...
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bioRxiv - Developmental Biology 2022Quote: ... Insertion of the product into the expressing vector was performed using In-fusion (Clontech, Mountain View, CA) protocol ...
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Evolutionary conserved aspects of animal nutrient uptake and transport in sea anemone vitellogenesisbioRxiv - Evolutionary Biology 2022Quote: ... They were then incubated with primary antibody (rabbit anti-DsRed, 1:100, Takara Bio Clontech, order nr. 632496) overnight at 4°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and each RNA concentration was measured by quantitative PCR after reverse transcription using PrimeScript One Step RT-PCR Kit (TaKaRa) with each specific primer (Table S2).
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bioRxiv - Evolutionary Biology 2022Quote: ... and each RNA concentration was measured by quantitative PCR after reverse transcription using PrimeScript One Step RT-PCR Kit (TaKaRa, Japan) with specific primers (Table S2) ...
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Evolutionary conserved aspects of animal nutrient uptake and transport in sea anemone vitellogenesisbioRxiv - Evolutionary Biology 2022Quote: ... They were then incubated with primary antibody (rabbit anti-DsRed, 1:100, Takara Bio Clontech, order nr. 632496) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... 2 μl of the pre-RT-PCR2 (PrimeScript™ II Reverse Transcriptase, Takara Bio) mix was added to each well ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were purified using Capturem™ His-Tagged Purification kit (Takara Bio), then dialyzed by PBS buffer overnight ...
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bioRxiv - Immunology 2022Quote: ... PrimeSTAR DNA polymerase (Takara Bio) and custom primers were used ...
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bioRxiv - Immunology 2022Quote: ... LentiX-293T cells (Takara Bio) and 293T cells were maintained in culture with Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Immunology 2022Quote: ... by In-Fusion® technology (Takara Bio). pcDNA3.4 expression plasmids encoding SARS-CoV-2 S proteins with human codon optimization and 19 a.a deletion of C-terminus (C-del19 ...
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bioRxiv - Immunology 2022Quote: ... cDNA was reverse-transcribed with MMLV reverse transcriptase (Clontech) using oligo-dT20 primers ...
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Endothelial transmigration hotspots limit vascular leakage through heterogeneous expression of ICAM1bioRxiv - Immunology 2022Quote: ... filtered (0.45 micron) and concentrated with Lenti-X concentrator (Clontech, 631232). Virus was added to HUVEC or BOECs 1:250 to 1:500 ...
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bioRxiv - Microbiology 2022Quote: ... 2013) In which, once double digestion with MboI and MseI enzymes (Fermentas, Lithuania) and ligation by T4 DNA ligase (Takara Bio), PCRs by limited adaptors and primers were conducted (all used adaptors and PCR primers listed in Table S4).
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A nepenthesin insert allosterically controls catalysis in the malaria parasite protease plasmepsin VbioRxiv - Microbiology 2022Quote: ... and inserted by Infusion (Clontech) into pEOE-AttP cut with AatII and AvrII ...
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bioRxiv - Biophysics 2022Quote: ... Unique clones for each COP were sub-cloned in pRH2.2 using the In-Fusion Cloning kit (Takara Bio). Sequence-verified COPs were transformed into E ...
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bioRxiv - Biophysics 2022Quote: ... Resulting fragments were cloned into linearized FM5-mCh or FM5-GFP constructs using In-Fusion Cloning kit (Takara). The resulting plasmids were sequenced to confirm correct insertion.
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bioRxiv - Biophysics 2022Quote: ... Virus was concentrated using Lenti-X Concentrator (Takara) and resuspended in DPBS before being applied to neurons.
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bioRxiv - Molecular Biology 2022Quote: ... Purified product was digested with BamHI and SalI restriction endonucleases (TAKARA, Japan) and sub cloned in BamHI and SalI site of pCDNA3.1/myc-His(A ...
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bioRxiv - Biophysics 2022Quote: ... We obtained cDNA from poly(A) RNA of each sample using a SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio Inc., Shiga, Japan), following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was clarified by centrifugation (292,055 g, 60 min, 4°C) and bound to 2 ml of TALON IMAC resin (Clontech) overnight with 10 rpm rotation in the presence of 20 mM imidazole and NaCl added up to 800 mM ...
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bioRxiv - Cell Biology 2022Quote: ... transfected iPSCs were selected for 48 hours with 0.5 μg/mL puromycin (Takara).
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The spatial landscape of progression and immunoediting in primary melanoma at single cell resolutionbioRxiv - Cancer Biology 2022Quote: ... The sequencing libraries were prepared with ThruPLEX DNA-seq Kit (Takara Bio). The resulting libraries were characterized by using the Qubit dsDNA HS Assay Kit and BioAnalyzer 2100 High Sensitivity DNA Kit ...
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bioRxiv - Immunology 2022Quote: Cell death was measured by quantification of the lactate dehydrogenase (LDH) release into the cell supernatant using LDH Cytotoxicity Detection Kit (Takara). Briefly ...
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bioRxiv - Bioengineering 2022Quote: ... Blood samples were first lysed by Trizol reagent (TAKARA). Then ...
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The spatial landscape of progression and immunoediting in primary melanoma at single cell resolutionbioRxiv - Cancer Biology 2022Quote: Reverse transcription and cDNA amplification were performed using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio, Kusatsu, Shiga, Japan). The resulting amplified cDNA libraries were assessed for DNA concentration using the Qubit dsDNA HS Assay Kit (ThermoFisher ...
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bioRxiv - Plant Biology 2022Quote: ... The UGI was PCR amplified using primers UGI-F1/R1 and cloned into EcoRI site of the CmYLCV-A3A-RAD51-nCas9 vector via the in-fusion cloning method (Takara Bio) to construct the final CBE vector ...
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bioRxiv - Plant Biology 2022Quote: ... and SbfI/BspEI-digested vector was performed using the in-fusion cloning method (Takara Bio) to make an intermediate vector CmYLCV-A3A-RAD51-nCas9 ...
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bioRxiv - Plant Biology 2022Quote: ... and EcoRI/XbaI-digested vector was performed using the in-fusion cloning method (Takara Bio) to make vector PC-ABE8e ...
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bioRxiv - Biochemistry 2022Quote: ... The mutant derivatives of this vector were generated with the PrimeSTAR Mutagenesis Basal Kit (TAKARA-bio).
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bioRxiv - Synthetic Biology 2022Quote: ... A total of 5 ml of Talon Metal Affinity Resin (Takara Bio USA) was added to the supernatant and mixed overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... SYBR Green Premix Ex Taq II (Takara, RR820A) was used to analyze the quantitative RT-PCR (RT-qPCR ...
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bioRxiv - Developmental Biology 2022Quote: ... Prime Script RT Master Mix (Takara, RR036A) was used to reverse transcribe 1 µg of total RNA into cDNA ...
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bioRxiv - Developmental Biology 2022Quote: ... 1µl diluted cDNA was used as the template for each reaction with Emerald Amp GT PCR Master Mix (Takara, RR310). After appropriate amplification cycles ...
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bioRxiv - Cell Biology 2022Quote: ... mouse GFP antibody (Clontech, clone JL8, used at 1:4,000) was obtained from Takara (Saint-Germain-en-Laye ...
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bioRxiv - Cell Biology 2022Quote: ... and GFP-KDEL sequence were expressed from C1/N1 vector series (Clontech) and have been previously described (Jongsma et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Vimentin was subcloned between EcoRI and BamHI sites of the C1-IRES-GFP vector of C1-GFP/RFP vector (Clontech). Mutants were created by standard (mutagenesis ...
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bioRxiv - Biochemistry 2022Quote: ... the cDNA of PFK1-mEGFP was amplified by PCR and cloned into pTRE3G vector (Clontech). The pTRE3G vector containing PFK1-mEGFP was then co-transfected into HeLa Tet-On 3G cells (Clontech ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were washed twice with 20 mL of D-PBS (Nacalai Tesque) and resuspended in CELLBANKER 1 (Takara Bio) at 1 × 107 cells/mL ...
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bioRxiv - Biochemistry 2022Quote: ... The molar concentration of the sequencing library was determined using the library quantitation kit from Takara Bio Inc ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-EGFP (Clontech, 632569) and anti-β-actin (Santa Cruz ...
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bioRxiv - Biochemistry 2022Quote: Total RNA was isolated using TRIzol Reagent (Takara, USA) and total RNA was harvested at different times after induction ...
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bioRxiv - Bioengineering 2022Quote: ... Lenti-X 293T cells were purchased from Takara and cultured according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: HEK293T cells were harvested for RNA extraction using RNeasy Plus Mini Kit (Qiagene) or NucleoSpin RNA Plus Kit (Takara). RNA concentration was measured by nanodrop and 500ng to 1000ng total RNAs were used for reverse transcription in 10 ul reaction volume by High-Capacity RNA-to-cDNA kit (ThermoFisher) ...
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bioRxiv - Bioengineering 2022Quote: ... a master mix consisted of 150 μL DNA Polymerase (Titanium Taq; Takara), 1 mL of 10x buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Xfect from Takara Bio is characterized as a biodegradable transfection polymer suitable for both CHO and HEK ...
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bioRxiv - Genomics 2022Quote: Induction of the expression of Asmt by the addition of doxycycline (0.5 µg/mL; Cat# 631311, Takara Bio) for 24 h and total RNA was extracted using Trizol reagent ...