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Citations for Takara Bio :
6501 - 6550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: Mitochondrial network morphology was assessed transfecting hPASMC grown on a 60 mm plate to 50% confluence with 1 μg pDsRed2-mito expression vector (Clontech Laboratories, 632421) and 2 μL Lipofectamine™ 2000 (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... and Lenti-X concentrator (Takara) was added ...
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bioRxiv - Cell Biology 2022Quote: For HEK293T transduction: HEK293T cells (LentiX 293T cell line, Takara) were transfected in 10 cm dishes with packaging plasmid psPAX2 ...
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bioRxiv - Cell Biology 2022Quote: For primary neuron transduction: HEK293T cells (LentiX 293T cell line, Takara) for lentiviral packaging were expanded to 70-85% confluency in DMEM Glutamax (+4.5 g/L D-glucose ...
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bioRxiv - Cell Biology 2022Quote: ... 6-well plates of 70% confluent Lenti-X 293T(Clontech) cells were transfected with 1.5 μg of transfer vector ...
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bioRxiv - Cell Biology 2022Quote: ... Lentiviral media was then kept at 4°C before 100x concentration using LentiX concentrator (Takara #631232) and resuspension of virus in organoid seeding media ...
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bioRxiv - Cell Biology 2022Quote: ... and Lenti-X 293T cells were from Clontech. No commonly misidentified cell line was used in this study ...
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bioRxiv - Cell Biology 2022Quote: ... RT-qPCR was performed with TB Green Premix Ex Taq II (Tli RNaseH Plus) (TaKaRa) on the StepOnePlus Real-time PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... and complementary DNA of miR was synthesized using the Mir-X miRNA First-Strand Synthesis Kit (TaKaRa). RT-qPCR was performed with TB Green Premix Ex Taq II (Tli RNaseH Plus ...
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bioRxiv - Cell Biology 2022Quote: ... and RT was performed using the PrimeScript RT Master Mix (TaKaRa). For the RT-qPCR analysis of miR ...
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bioRxiv - Cell Biology 2022Quote: ... Using the PrimeScript RT Master Mix (Takara Bio), we reverse transcribed total RNA ...
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bioRxiv - Cell Biology 2022Quote: ... then into pShuttle-CMV with EcoRV using the In-Fusion HD Cloning Plus System kit (Takara Bio USA). These were linearized with Pme1 and cotransformed with pAdEasy-1 into BJ5183 cells ...
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bioRxiv - Cell Biology 2022Quote: ... mCherry-Lc3b and GFP-Lc3b were made by cloning Lc3b into the EcoRI and BamHI sites of pEGFP-C1 (Clontech Laboratories, CA, USA). GST-Lc3b was constructed by cloning Lc3b into the BamHI and SalI sites of pGEX-4T-1 (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... and subsequently also in pBApo-EF1α Neo (Takara Bio Inc. ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was reversed transcribed to cDNA by the PrimeScript DNA Reverse Transcription kit (Takara, Japan). PCR was conducted by a 7500 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR fragment was prepared with a normal PCR protocol using KOD-Plus-Neo DNA polymerase (KOD-401, Toyobo, Japan) or ExTaq DNA Polymerase (RR001, Takara, Japan), both of which had 3’-5’ exonuclease activities.
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bioRxiv - Cell Biology 2022Quote: ... and oligo(T)18 primers (Takara). Relative transcript amounts were measured by SYBR-green quantitative PCR (qPCR ...
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bioRxiv - Cell Biology 2022Quote: ... unique protospacers targeting the 50-bp window surrounding the 1st codon of each gene were annealed and cloned into the pCAB_minimal via BbsI digestion and ligation (New England BioLabs # R3539S, Takara #6023) using standard protocols 48 ...
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bioRxiv - Cell Biology 2022Quote: cDNA was generated from single cells in the 96-well plate using the SmartSeq v4 kits (Takara Bio) using 1/4th volume reactions dispensed using a Mantis dispenser (Formulatrix) ...
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Targeted degradation of PCNA outperforms stoichiometric inhibition to result in programed cell deathbioRxiv - Cell Biology 2022Quote: Human embryonic kidney T-REx 293 cells which express the tetracycline repressor protein were purchased from Thermo Fisher Scientific and cultured in Eagle’s Minimum Essential Medium (MEM) GlutaMAX™ supplemented with 10% Tet system approved FBS (Clontech) and 5 μg/mL blasticidin ...
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bioRxiv - Cell Biology 2022Quote: ... PCR-amplified from pEGFP-C1 (Clontech), at the NheI-XhoI sites of the pCI-Neo vector and then by adding the fragment encoding GIGYF2 at the XhoI-NotI sites ...
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bioRxiv - Cell Biology 2022Quote: ... Lentiviral particles were further concentrated using Lenti-X concentrator (Clontech) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: All plasmids used were generated using the In-Fusion cloning system (Takara) with primers containing 15 bp 5’ flanking regions complementary to the free ends of the cloning vector ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% FBS (Clontech), 100 units/mL penicillin ...
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bioRxiv - Cell Biology 2022Quote: ... Viruses were titered using the Lenti-X p24 rapid titer kit (Takara). SH-SY5Y were transduced in 96-well plates at multiplicity of infection (MOI ...
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bioRxiv - Cell Biology 2022Quote: ... 10X concentrated using Lenti-X concentrator (Takara Bio Inc, Japan), and used to infect Stat3−/− MEFs in the presence of Transdux Max (System Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... using In-Fusion® Snap Assembly (Takara Bio # 638947) and the following primers ...
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bioRxiv - Cell Biology 2022Quote: ... Digestion and ligation to BamHI and SalI-linearized pColdI plasmid (Takara, Shiga, Japan) were performed ...
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bioRxiv - Cell Biology 2022Quote: ... Retrovirus or lentivirus was concentrated using Lenti-X Concentrator (Takara Bio 631231) using the manufacturer’s protocol.
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bioRxiv - Cell Biology 2022Quote: cDNA synthesis and amplification was performed using a modified protocol for the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... LC NLS deletion (Δ417-422) was generated by KOD One PCR amplification and the In-Fusion HD Cloning Kit (Clontech). The NLS of SUN2 (KDSPLRTLKRKSSNMKRL ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was generated with a PrimeScript RT Kit (Takara, Otsu, Shiga, Japan). Q-PCR was conducted to detect RNA amounts using FastStart Universal SYBR Green Master (ROX ...
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bioRxiv - Developmental Biology 2022Quote: ... Three transformation reactions were performed with 2 µl of the reaction mixture in 50 µl Stellar− Competent Cells (636763, Takara) each according to the manufacturer’s manual ...
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bioRxiv - Developmental Biology 2022Quote: ... were concentrated using Lenti-X− Concentrator (631232, Takara) and pellets were dissolved in 400 µl PBS.
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bioRxiv - Developmental Biology 2022Quote: ... Lenti-X− Concentrator (631231, Takara) was added to the supernatant at 1 ...
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bioRxiv - Genomics 2022Quote: ... the cDNAs were amplified with an appropriate number of PCR cycles using LA Taq polymerase (Takara), and then the reactions were treated with 200 U/mL of Exonuclease I (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... The NLS of SUN2 (KDSPLRTLKRKSSNMKRL) (Turgay et al., 2010) cDNA was amplified by the PrimeSTAR HS DNA Polymerase (Takara) from pEGFPN3-SUN2 (Nishioka et al. ...
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bioRxiv - Cell Biology 2022Quote: ... pVSV-G (PT3343-5, Clontech) and psPAX2 (Addgene plasmid #12260 ...
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bioRxiv - Developmental Biology 2022Quote: ... the PCR master mix was prepared with Z-Taq (Takara R006B)(see Table S1 for genotyping primers ...
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bioRxiv - Developmental Biology 2022Quote: ... Primary antibodies were anti-PLP (N-terminus; 1:5000, Rogers, 2008) anti-GFP (JL8; 1:2000 – 5000; Clontech); anti-alpha-Tubulin (DM1A ...
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Nulliparity affects the expression of a limited number of genes and pathways in Day 8 equine embryosbioRxiv - Developmental Biology 2022Quote: ... Messenger RNAs were reverse transcribed and amplified using the SMART-Seq V4 ultra low input RNA kit (Clontech, France) according to the manufacturer recommendations ...
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bioRxiv - Developmental Biology 2022Quote: ... linearized with XbaI restriction enzyme (Takara, #1093B) and transcribed with a DIG-labelling Kit (Roche ...
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bioRxiv - Developmental Biology 2022Quote: ... with SYBR Premix Ex Taq (Takara). The mouse Rplp0 gene was used as an internal control ...
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bioRxiv - Developmental Biology 2022Quote: ... and then used for cDNA synthesis with PrimeScript RT Master Mix (Takara). Quantitative PCR analyses were performed using the LightCycler 96 System (Roche Applied Science ...
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bioRxiv - Developmental Biology 2022Quote: ... the IRES sequence from pLVX-IRES-Puro (Clontech; 632186), and sfGFP (superfolder GFP ...
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bioRxiv - Developmental Biology 2022Quote: ... into the lentiviral backbone pTetOne (Takara; 631018) linearized by EcoRI/BamHI digestion (NEB ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNAs were prepared on chip using the SMARTer Ultra Low RNA kit for Illumina (Clontech). ERCC (External RNA Controls Consortium ...
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bioRxiv - Developmental Biology 2022Quote: ... and titer estimation (Lenti-X Expression System, Clontech). An AAV virus that constitutively expresses eGFP from CMV promoter (Addgene ...
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bioRxiv - Developmental Biology 2022Quote: ... a lentiviral approach was developed using the Lenti-X Tet-On 3G Inducible Expression System (Clontech). The ORF encoding Fgfr2iiib (Origene ...
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bioRxiv - Developmental Biology 2022Quote: ... Two microlitres of 10-fold diluted cDNA was used as the template and amplified with TB Green® Premix Ex Taq (Takara) in a 20 μl volume quantitative PCR ...