Labshake search
Citations for Takara Bio :
6451 - 6500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... and 2 μg/ml Doxycycline (Clontech; Cat. No. 631311). iTF-iPSCs were counted and seeded onto double coated plates (Poly-D-Lysine-precoated Bio plates (Corning ...
-
bioRxiv - Biochemistry 2022Quote: ... Quantitative PCR was alternatively performed for the cDNA using the SYBR® Premix Ex Taq™ (Tli RNase H Plus) (TAKARA-Clontech) using a QuantStudio5 system (Applied Biosystems) ...
-
bioRxiv - Biochemistry 2022Quote: ... Quantitative PCR was alternatively performed for the cDNA using the SYBR® Premix Ex Taq™ (Tli RNase H Plus) (TAKARA-Clontech) using a QuantStudio5 system (Applied Biosystems) ...
-
bioRxiv - Neuroscience 2022Quote: ... PCR reaction consisted of PrimeSTAR Max Premix 1x (Takara Bio), 0.4 μM of each primer and 30-100 ng DNA template ...
-
bioRxiv - Developmental Biology 2022Quote: ... Fluorescent versions of this reporter were prepared by replacing the AP tag with either RFP or with a destabilized version of GFP (pGL3-RARE-SV40-RFP and pGL3-RARE-SV40-d2EGFP, respectively (Clontech). Wnt activity was measured with 12XTOPFLASH-d2EGFP (Rios et al. ...
-
bioRxiv - Neuroscience 2022Quote: ... rabbit anti-DsRed (1:100, Clontech).
-
bioRxiv - Neuroscience 2022Quote: ... (Clontech Lab ...
-
bioRxiv - Plant Biology 2022Quote: ... bHLH100 and bHLH101 gene were PCR amplified and cloned into pGADT7-Rec vector (Takara) following manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2022Quote: ... 1500 bp upstream sequence from +1 site of both AtNRAMP4 and AtPIC1 genes were amplified by PCR and subsequently cloned into the KpnI and SacI RE sites of the pAbAi vector (Takara). On the other hand ...
-
bioRxiv - Plant Biology 2022Quote: ... The recombinant constructs was transformed into Y1H gold strain and Y1H assay was performed using Matchmaker® Gold Yeast One-hybrid screening system (Takara) following the manufacturer’s protocol ...
-
bioRxiv - Genomics 2022Quote: ... This was followed by a Reverse transcription reaction (SmartScribe kit Clontech) for 1 hour at 42°C and 70°C for 15 minutes ...
-
bioRxiv - Immunology 2022Quote: ... Both constructs were synthesized by IDT (Coralville, IA, USA) and cloned into the pIRESpuro3 vector (cat# 631619, Takara Bio, USA) using EcoRI and BamHI restriction enzymes (R0101S and R0136S respectively ...
-
bioRxiv - Synthetic Biology 2022Quote: ... B/B dimerizer (AP20187 ligand; TaKaRa 635058) was added to a final concentration of 500 nM in the well ...
-
bioRxiv - Developmental Biology 2022Quote: ... and once in 5ml NDiff227 medium (Takara Y40002) pre-conditioned in normoxia or hypoxia ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 500 nM of the B/B dimerizer (TaKaRa 635058) was added 2 hours before the start of the assay (or an Ethanol vehicle control was added) ...
-
bioRxiv - Cancer Biology 2022Quote: ... pre-cleared by centrifugation at 1000xg for 5 min and concentrated by 40X using Lenti-X (Takara) following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... and incubated with a mix of up to three primary antibodies simultaneously diluted in PBST with 1 % NDS overnight at room temperature with the following primary antibodies: rabbit anti-dsRed (1:1000; Clontech, AB_10013483), chicken anti-GFP (1:1000 ...
-
bioRxiv - Cancer Biology 2022Quote: ... MDA-MB-231 (ATCC) and lenti-X 293T cells (Takara Bio) were grown in DMEM (Sigma ...
-
bioRxiv - Cancer Biology 2022Quote: 1.5 μL Ex Taq DNA polymerase (Takara Bio)
-
bioRxiv - Microbiology 2022Quote: ... Membranes were hybridized in ExpressHyb Hybridization Buffer (ClonTech) to random-primed 32P-labeled DNA probes (RadPrime DNA labelling system ...
-
bioRxiv - Immunology 2022Quote: ... and fused to the C-terminus of mNeonGreen-F-Tractin using In-Fusion cloning (Takara). As a control ...
-
bioRxiv - Plant Biology 2022Quote: ... and subjected to reverse-transcription using a PrimeScript™ RT Reagent Kit (Takara Biotechnology ...
-
bioRxiv - Plant Biology 2022Quote: ... https://www.thermofisher.com/) with SYBR® Premix Ex Taq™ (Takara), following the manufacturer’s instructions ...
-
bioRxiv - Physiology 2022Quote: ... Messenger RNAs were reverse transcribed and amplified using the SMART-Seq V4 ultra low input RNA kit (Clontech, France) according to the manufacturer recommendations ...
-
bioRxiv - Cancer Biology 2022Quote: ... Prior to RNA extraction (Takara Nucleospin), tumors were crushed using ice-cold mortar and pestle under sterile conditions ...
-
bioRxiv - Microbiology 2022Quote: ... Addgene_12259) into 293XT cells (Takara Holdings). Lentivirus containing supernatant was harvested at 48 ...
-
bioRxiv - Cell Biology 2022Quote: ... The gene encoding TERT and mVenus was inserted into pSBbi vector using the In-Fusion cloning kit (Takara Bio, Shiga, Japan). The R3E/R6E mutation was introduced using the KOD mutagenesis kit (Toyobo ...
-
bioRxiv - Systems Biology 2022Quote: ... The blocked membrane was incubated overnight at 4 ℃ in primary antibodies: mouse polyclonal anti-Cas9 (Takara Bio, cat. no. 632607), mouse monoclonal anti-β-actin (8H10D10 ...
-
bioRxiv - Neuroscience 2022Quote: ... for QF2 lines and NucleoBond Xtra Midi Endotoxin-Free plasmid kit (Clontech 740420.50) for Split-QF2 lines and eluted in ultrapure water prior to injection ...
-
bioRxiv - Molecular Biology 2022Quote: ... The viral titer was determined using Lenti-X™GoStix™ (Takara Bio Cat. #: 631280). HEK293T cells were transduced with lentivirus encoding the ABE reporter at varying dilutions (1:10 ...
-
Map7D2 and Map7D1 facilitate microtubule stabilization through distinct mechanisms in neuronal cellsbioRxiv - Cell Biology 2022Quote: An RNA blot membrane (Clontech) was hybridized with the 32P-labeled full coding sequence of rMap7D2 ...
-
bioRxiv - Cell Biology 2022Quote: Single guide RNAs (sgRNAs) in the pX330 vector (1 μg) were mixed with EGFP (0.1 μg; Clontech) and co-transfected into MAP4K3 k.o ...
-
bioRxiv - Cell Biology 2022Quote: ... and kidney 293 GP cells (Clontech, Mountain View, CA, USA) were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
-
bioRxiv - Cell Biology 2022Quote: ... EcoRI-BglII digestion product of pAc-GFPC1-Sec61β was ligated into pmCherry-C1 vector (made by substituting mCherry for EGFP in pEGFP-C1 (Clontech) by AgeI-XhoI digestion) ...
-
bioRxiv - Cell Biology 2022Quote: ... Real-time PCR was performed using the SYBR pPCR premix Ex Taq (Takara) or LUNA Universal qPCR Master Mix (NEB ...
-
bioRxiv - Genomics 2022Quote: ... Paris) were prepared following the kit manufacturer’s recommendations (SMART-Seq v4 Ultra Low Input RNA Kit from Takara). Samples were sequenced on a NextSeq 500 Illumina device with MidOutPut cartridge to generate a total of 2 × 87 M reads of 75 bp ...
-
bioRxiv - Microbiology 2022Quote: ... Cloning was carried out with Infusion (Clontech) and NEBuilder HiFi (NEB) ...
-
bioRxiv - Cancer Biology 2022Quote: ... pEGFP (Clontech), are described elsewhere ...
-
bioRxiv - Microbiology 2022Quote: ... A maximum of 500 ng of RNA were reverse transcribed with both oligo dT and random primers using a PrimeScript RT Reagent Kit (Perfect Real Time, Takara Bio Inc.) in a 10 µL reaction ...
-
bioRxiv - Microbiology 2022Quote: ... HeLa Tet-Off cells (Clontech) and HeLa Flp-In TREx GFP-Dcp1a cells (a generous gift from Dr ...
-
bioRxiv - Biochemistry 2022Quote: ... The supernatant stirred with 10 mL of TALON metal affinity resin (Takara Bio US) equilibrated in Buffer A (480 mM NaCl ...
-
bioRxiv - Biochemistry 2022Quote: ... Miniprepped DNA was transformed into STELLAR Competent Cells (Clontech) and plated on carbenicillin LB agar plates (as per pPNL6’s resistance marker) ...
-
bioRxiv - Biochemistry 2022Quote: ... as used before.11 TSC were cotransfected with GFP-expressing plasmid peGFP-CI (Clontech) and sorted for GFP-positive cells in a 96 well plate at the central research facility cell sorting at MHH ...
-
bioRxiv - Biochemistry 2022Quote: ... were purchased from Biomatik and cloned into BstBI-digested PBRM1-TetO-FUW using the ligation-free In-Fusion HD cloning kit (Takara). The construct was packaged into lentivirus using HEK293T cells and delivered into target cells together with pLenti CMV rtTA3 Hygro (w785-1 ...
-
bioRxiv - Biochemistry 2022Quote: ... and/or pRFP (Clontech) by techniques as described earlier (6,10,26).
-
bioRxiv - Bioengineering 2022Quote: ... and TP–mAG were ligated into the open circular pET-29b(+) using the In-Fusion HD Cloning Kit (TaKaRa) to generate the recombinant protein expression vectors ...
-
bioRxiv - Bioengineering 2022Quote: ... PCR was performed in the TaKaRa PCR Thermal Cycler Dice Touch (TaKaRa, Shiga, Japan) using the Tks Gflex DNA Polymerase (TaKaRa) ...
-
bioRxiv - Bioengineering 2022Quote: ... using the Tks Gflex DNA Polymerase (TaKaRa). The synthetic genes coding TP–AG ...
-
bioRxiv - Bioengineering 2022Quote: ... PCR products were ligated to pMD18-T cloning vector (Takara Bio, Beijing, China) and then transformed in E.coli ...
-
Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsbioRxiv - Cell Biology 2022Quote: ... coli using the NucleoSpin Plasmid miniprep kit (Takara 740588). Transformants were sequenced to confirm successful cloning ...