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Citations for Takara Bio :
6401 - 6450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: All constructs were obtained with the In-Fusion HD cloning kit (Takara) and sequence verified ...
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bioRxiv - Genetics 2022Quote: ... PCR amplifications of 36 replicates of 90 pg gDNA and 8 DNA-negative PCR controls were carried out in a 25 µl reaction volume with buffer and dNTPs provided with the PrimeSTAR GXL polymerase (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... the coding sequence was cloned by InFusion (Clontech) in the plasmid pMT85 under the control of the promoter PTufA (42) ...
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bioRxiv - Microbiology 2022Quote: ... or the mEos3.2 coding sequence using InFusion (Clontech). Plasmids carrying the cassettes were checked by Sanger sequencing (Genewiz) ...
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bioRxiv - Immunology 2022Quote: ... Talon cobalt affinity resin (Takara) was added to the treated supernatants and allowed to incubate for 15 min with gentle shaking ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-dsRed (1:1000, Cat. # 632496, Takara Bio Europe SAS ...
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bioRxiv - Neuroscience 2022Quote: ... Viral-containing supernatants were collected and concentrated with Lenti-X Concentrator reagent (Clontech) according to the manufacturer’s protocol (100X concentration ...
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bioRxiv - Neuroscience 2022Quote: ... 0.2μM of each primer (see Table S1) and SYBR® Premix ExTaq™ (Takara) according to the manufacturer instructions ...
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bioRxiv - Plant Biology 2022Quote: ... RT-PCR was conducted using TB Green Premix Ex Taq II (Takara Bio) in a CFX96 Real Time PCR Detection System (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: Total RNAs were extracted from various tissues using the MiniBEST Plant RNA Extraction Kit (Takara Bio, Beijing, China). First-strand cDNAs were synthesized using TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix (TransGen Biotech ...
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bioRxiv - Molecular Biology 2022Quote: ... by RT-PCR (TaKaRa, RR014) and cloned by Gibson assembly into the vector pK27SUMO (expressing a 14His-tagged version of the yeast SUMO protein Smt3 ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA synthesized from 1 μg total RNA using PrimeScript™ RT reagent Kit with gDNA Eraser (Takara). Real-time PCR was performed using TB Green® Premix Ex Taq™ II (Tli RNaseH Plus ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was extracted with RNAiso Plus (Takara) following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2022Quote: The RNA samples extracted from the fruit flesh were reverse transcribed using the PrimeScript RT-PCR Kit (TaKaRa) and the oligo(dt)20 primer ...
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bioRxiv - Molecular Biology 2022Quote: About 1μg of RNAs were reverse transcribed into cDNAs using a PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Japan) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The amplified fragments were purified using a gel extraction kit (Vazyme, China) and cloned into a pMD-18T vector (Takara, Japan).
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bioRxiv - Molecular Biology 2022Quote: ... Equal volume RNAs were subjected to reverse transcription using the PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Japan). Distribution of mat mRNAs or mat-exon1 mRNAs in transfected cells was assessed using SBYR-Green (Takara ...
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bioRxiv - Molecular Biology 2022Quote: ... Distribution of mat mRNAs or mat-exon1 mRNAs in transfected cells was assessed using SBYR-Green (Takara, Japan)-based quantitative PCR analysis on Aligent Mx3005P ...
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bioRxiv - Molecular Biology 2022Quote: 5’ RACE was performed using SMARTer RACE 5’/3’ Kit (TAKARA #634858) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 5 μG (636224-Takara) using 1 μl from each ...
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bioRxiv - Microbiology 2022Quote: The Matchmaker® Gold Yeast Two-Hybrid System (TaKaRa) protocols were followed to verify the autoactivation of reporter genes by TaPHB-1 ...
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bioRxiv - Genetics 2022Quote: ... Quantitative PCR was performed with SYBR Premix Ex Taq II (TaKaRa Bio) or TB Green Premix DimerEraser (Takara Bio ...
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bioRxiv - Genetics 2022Quote: ... or TB Green Premix DimerEraser (Takara Bio) on a Mx3000P qPCR system (Agilent) ...
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bioRxiv - Microbiology 2022Quote: Lenti-X 293T cells (Clontech) and its derivative ...
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bioRxiv - Immunology 2022Quote: ... RT-PCR products were nested PCR-amplified with primers for IgG VH or IgG VL following the HS DNA polymerase kit protocol (Takara, TAK R010). The IgG VH and IgG VL nested PCR products were purified and next cloned into human IgG1 heavy chain and light chain expression vectors ...
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bioRxiv - Plant Biology 2022Quote: ... 1 μg total RNA was used for cDNA synthesis by oligo(dT)18 primer according to the manufacturer’s protocol (Takara). The resulting cDNA was subjected to relative quantitative PCR using the ChamQ™ SYBR qPCR Master Mix (Vazyme Biotech Co.,Ltd ...
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bioRxiv - Plant Biology 2022Quote: ... Growth was determined as described in the Yeast Two-Hybrid System User Manual (Clontech).
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bioRxiv - Genetics 2022Quote: ... GenTLE (from yeast) high recovery kit (Takara Bio). The genomic DNA concentration was measured using the Quantifluor® ONE dsDNA Dye System (Promega ...
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bioRxiv - Plant Biology 2022Quote: ... Amplified fragments were cloned into the TRV vector (pTRV2) (Lu et al., 2003) using the In-Fusion HD cloning kit (Clontech). Agrobacterium strains containing desired pTRV2 constructs were co-infiltrated with strains carrying pTRV1 at OD600=0.5 into N ...
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bioRxiv - Neuroscience 2022Quote: ... Collected lentiviral particles were purified with lenti-X concentrator (Clontech, Mountain View, CA).
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bioRxiv - Cell Biology 2022Quote: ... respectively (Clontech). For CRISPR-Cas9 mediated knockout of AMOT ...
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bioRxiv - Cell Biology 2022Quote: ... followed by cloning into pEGFP-C1 (Clontech). Plasmids encoding truncated KIF13B-HA fusions (Tail 1 and 16 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... ExTaq (Takara) was used following the manufacturer’s instructions with the following PCR conditions ...
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bioRxiv - Cell Biology 2022Quote: ... pLVX-IRES-Puro plasmid (Clontech) was digested with AgeI and NotI (FastDigest ...
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bioRxiv - Immunology 2022Quote: ... The following plasmids were generated by insertion of PCR-amplified fragments into the NotI-to-PmeI digested pEF-TAK-Flag using InFusion cloning (Clontech): pEF-TAK-Flag-UFL1 (GenBank ...
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bioRxiv - Cell Biology 2022Quote: ... rapalog (A/C Heterodimerizer, Takara), dynapyrazole A (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... The first-strand cDNA was synthesized using reverse transcriptase from the PrimeScript RT reagent kit (Takara Biotechnology, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... the total RNA was treated with DNase I (Takara Biotechnology, Japan) to remove any DNA contamination ...
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bioRxiv - Neuroscience 2022Quote: ... the 99-1820 bp fragment of the Rat Glt-1 plasmid (GenBankTM accession number X67857.1) was subcloned between the EcoRI and Xba restriction sites of pEGFP vector (Clontech). GFP-Rab4 ...
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bioRxiv - Biophysics 2022Quote: ... A Puromycin resistance plasmid (pPUR, Clontech, Cat No. 631601) was used as a selection marker ...
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bioRxiv - Biophysics 2022Quote: ... This plasmid expresses the PR isoform-B under a tetracycline controllable promoter (TetOff system, Clontech). To perform the SPT experiments ...
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bioRxiv - Biophysics 2022Quote: MCF7 Tet-off cells (Clontech, Cat No. 631154) were grown on Dulbecco Modified Eagle Medium (DMEM ...
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bioRxiv - Microbiology 2022Quote: ... non-structural genes were PCR amplified in three fragments from the pRepDVRluc plasmid [37] and all fragments were inserted into the PUb-MCS-2A-Puro plasmid [64] using In-Fusion cloning kit (Takara) to generate the PUb-NS(Ø ...
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bioRxiv - Plant Biology 2022Quote: ... (2019) using in-fusion cloning (Clontech, Madison, WI, USA). The resulting vectors were used to transform the 2012-1 isolate (containing AVR-Pias ...
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bioRxiv - Plant Biology 2022Quote: ... (2019) using in-fusion cloning (Clontech, Madison, WI, USA). Subsequently ...
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bioRxiv - Plant Biology 2022Quote: ... and cloned into pGADT7 (prey) or pGBKT7 (bait) vector (Clontech, Madison, WI, USA) that had been linearized by digestion with EcoRI and BamHI by in-fusion cloning ...
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bioRxiv - Plant Biology 2022Quote: ... and His (-HTLA) but containing 5-bromo-4-chloro-3-indolyl α-D-galactopyranoside (Clontech, Madison, WI, USA). As a control ...
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bioRxiv - Developmental Biology 2022Quote: ... Three nanograms of total RNA were used for amplification using the SMART-Seq V4 Ultra Low Input RNA kit (Clontech) according to the manufacturer’s recommendations (10 PCR cycles were performed) ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthesized from 500 ng total RNA using a Prime Script RT Reagent Kit (Takara Bio, Otsu, Japan). qRT-PCR was performed using a StepOne Real-time PCR Instrument (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... 0.25 μl RNAse inhibitor (40 U/μl, Clontech), 2 μl Superscript II First-Strand Buffer (5x ...