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Citations for Takara Bio :
6351 - 6400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... LRP10 sequence-verified cDNA was subcloned from the pcDNA™3.1-LRP10-V5-His-TOPO® plasmid (Quadri et al., 2018) into the pLVX-EF1α-IRES-mCherry plasmid (Takara Bio, 631987) via Gibson Assembly® (NEB ...
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bioRxiv - Biochemistry 2023Quote: The plasmids for producing XccOpgD mutants were constructed using a PrimeSTAR mutagenesis basal kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... along with 0.5 µg pMSCVpuro vector (Clontech) containing a puromycin resistance gene ...
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bioRxiv - Biochemistry 2023Quote: ... with optional concentrating lentivirus using Takara’s Lenti-X Concentrator (Takara 631231). Infection of PC3-AR-DTX3LKO cells were carried out in the growth medium (RPMI 1640 + 5% FBS + 1% Penicillin-Streptomycin solution ...
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bioRxiv - Biochemistry 2023Quote: ... Site-directed mutagenesis was performed using InFusion cloning (Takara). The sequence of all replicon plasmids was validated using Oxford nanopore sequencing (Plasmidsaurus).
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bioRxiv - Biochemistry 2023Quote: ... The His-tagged TEV protease was removed by binding to Co2+-charged TALON resin (Clontech) and the flow-through concentrated using a 100 kDa molecular weight cut off concentrator (Corning) ...
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bioRxiv - Bioengineering 2023Quote: ... Quantitative RT-PCR was performed using TB Green Premix Ex Taq II (Takara Bio Inc.) in an Applied biosystems Quantstudio 5 ...
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bioRxiv - Bioengineering 2023Quote: ... Monoclonal Antibody (JL-8) (632381; Clontech) in 1:2,000 dilution ...
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bioRxiv - Bioengineering 2023Quote: ... 0.2U/µL RNase inhibitor (Takara), 0.1% NP-40 ...
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bioRxiv - Cell Biology 2023Quote: ... concentrated with Lenti-X concentrator (Clontech; 631232) and used to infect HUVECs ...
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bioRxiv - Immunology 2023Quote: ... The supernatants were collected and mixed with Lenti-X Concentrator (Takara Bio USA, Inc) at a 1:3 ratio ...
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bioRxiv - Microbiology 2023Quote: ... using the In-Fusion HD Cloning Kit (Takara), following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... Total RNA was isolated from cells using Trizol reagent (Takara, Otsu, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... Activated T cells were transduced on day 1 after stimulation using combinations of PEG-precipitated retroviral concentrates encoding different constructs adsorbed onto non-tissue culture treated 6-well plates coated with anti-CD3/CD28 2 μg/mL each and retronectin 20 μg/mL (Takara, T100B) at 1-2×106 cells/well ...
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bioRxiv - Biophysics 2023Quote: ... The cDNA encoding Keratin 14 R125C-YFP was a kind gift of Thomas Magin (University of Leipzig, Germany) and was cloned into a retroviral vector (pTRE, Takara Clontech). Retrovirus was generated as described in [44] and transduced into MDCK cells ...
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bioRxiv - Evolutionary Biology 2023Quote: ... HeLa cells were co-transfected to express each of EGFP-fused MK-D1 proteins with an endoplasmic reticulum (ER) localizing mCherry construct using the Xfect transfection reagent (Takara Bio). At 24 h post-transfection ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Mycoplasma contamination in cell cultures was routinely tested using the PCR mycoplasma detection set (Takara Bio). At approximately 70% confluence ...
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bioRxiv - Genomics 2023Quote: ... cfChIP-seq libraries were prepared with ThruPLEX DNA-Seq Kit (Takara Bio, cat# R400675) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti-dsred (Takara), rabbit anti-GFP (Proteintech) ...
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bioRxiv - Genetics 2023Quote: ... Yeast two-hybrid experiments were conducted using Matchmaker Yeast Two-Hybrid Kit (Clontech) following the product instruction ...
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bioRxiv - Evolutionary Biology 2023Quote: ... which were carried out by Advantage® 2 Polymerase (Takara Bio). Final products were outsourced for Sanger sequencing (HyLabs ...
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bioRxiv - Evolutionary Biology 2023Quote: ... SMARTer® RACE 5’/3’ Kit was used (Takara Bio, Japan). Prior to the reaction ...
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bioRxiv - Cell Biology 2023Quote: ... Virus containing media was collected from the LentiX-293T cells and concentrated using Lenti-X Concentrator (Takara Bio). pLVX-Tet3G virus and polybrene was added to L6-myoblast cells and cells were positively selected using neomycin to create Tet3G expressing cells ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was reverse transcribed to cDNA using PrimeScript Reverse Transcriptase(Takara) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... IRF3 and IRF3/5D vectors were constructed by inserting the corresponding gene fragments into the pCMV-Myc vector (Clontech). All the constructs were validated by Sanger DNA sequencing.
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bioRxiv - Cancer Biology 2023Quote: ... and clonally isolated p53 KO cell lines were electroporated with 3 µg p53 firefly luciferase reporter plasmid pp53-TA-Luc (Clontech/Takara) and 0.3 ug renilla luciferase control plasmid pRL-CMV (Promega ...
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bioRxiv - Cancer Biology 2023Quote: ... and clonally isolated p53 KO cell lines were electroporated with 3 µg p53 firefly luciferase reporter plasmid pp53-TA-Luc (Clontech/Takara) and 0.3 ug renilla luciferase control plasmid pRL-CMV (Promega ...
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bioRxiv - Immunology 2023Quote: ... and Lenti-X cells (TaKaRa Bio) were cultured at 37 °C with a humidified atmosphere of 5% CO2 in complete media containing high glucose ...
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bioRxiv - Physiology 2022Quote: ... LV particles were concentrated from the filtered supernatants using a Lenti-X concentrator kit (Takara, 631232) following manufacturer’s protocol.
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bioRxiv - Cell Biology 2022Quote: ... the PCDH15-CD2 isoform cytoplasmic domain was cloned using the HindIII and BamHI restriction sites of the pmCherry-C2 vector (Clontech), resulting in soluble protein tagged with cherry at its N-terminus.
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bioRxiv - Biochemistry 2022Quote: ... plasmids encoding trcP mutants were generated with the PrimeSTAR Mutagenesis Basal Kit (TAKARA-bio).
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Three Distinct Transcriptional Profiles of Monocytes Associate with Disease Activity in SSc PatientsbioRxiv - Genomics 2022Quote: ... Full-length RNA-seq libraries from CM and skin macrophages were prepared from ∼3 ng of total RNA using SMART-Seq v4 Ultra Low Input RNA Kit (Clontech Laboratories) followed by Nextera XT protocol (Illumina) ...
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bioRxiv - Immunology 2022Quote: ... 0.5 µg of the isolated RNA was reverse transcribed to cDNA using an oligo dT primer and 12.5 units of reverse transcriptase mix (PrimeScript RT Reagent Kit, TaKaRa Bio, Saint-Germain-en-Laye, France). cDNA corresponding to 10 ng total RNA served as the template in a real-time PCR ...
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bioRxiv - Microbiology 2022Quote: ... Linearized vector and inserts were ligated using In-Fusion® HD Cloning Kit (Clontech, Takara, Japan) and transformed into StellarTM competent cells ...
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bioRxiv - Microbiology 2022Quote: ... 10 μL of Clone AMP (Takara), and 300 nM each of primers ks1 and mf83 ...
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bioRxiv - Microbiology 2022Quote: ... cDNA libraries were prepared using the SMARTer® Universal Low Input RNA Kit for Sequencing (Takara Bio), without rRNA depletion ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... mtDNA copy number was determined by quantitative PCR with Human mitochondrial to nuclear DNA ratio kit (Takara Bio USA, 7246).
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bioRxiv - Pathology 2022Quote: Total RNA was extracted from skin or cell lysates using TRIzol Reagent (RNAiso Plus, TaKaRa). From isolated RNA ...
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bioRxiv - Pathology 2022Quote: ... with SYBR Green PCR master mix (Takara, Dalian, China) and gene-specific primers ...
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bioRxiv - Neuroscience 2022Quote: ... a sequence region of 5,000 bp between SnaBI and AgeI restriction sites was amplified using the HiFi amplification kit (Clontech, Takara Bio Europe ...
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bioRxiv - Neuroscience 2022Quote: ... Using the In Fusion Cloning Kit (Clontech) the 5,000 bp amplicon carrying the mutation was inserted into the full-length Reelin-coding plasmid digested with SnaBI and AgeI.
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bioRxiv - Synthetic Biology 2022Quote: ... All constructs were built via in-fusion cloning (Clontech #ST0345) and sequence verified before use ...
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bioRxiv - Synthetic Biology 2022Quote: Genomic DNA was extracted from sorted T cells using the Macherey-Nagel NucleoSpin Tissue XS kit (Takara #740901.250). DNA encoding the CAR costimulatory domain was amplified from the extracted genomic DNA using the forward primer 5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNNACTGGTTATCACCCTTTA CTGC-3’ (Integrated DNA Technologies ...
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bioRxiv - Synthetic Biology 2022Quote: ... A middle-copy (∼10-30 copies) episomal plasmid pGADT7 (Takara Bio Inc.) was used to increase the concentration of proteins to buffer for the effects of intrinsic molecular noise and selected using different auxotrophic selection markers (Leucine ...
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bioRxiv - Plant Biology 2022Quote: The Y2H assays were performed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech, Mountain View, CA, USA). The full-length and truncated coding regions of WL1 were amplified and cloned into the yeast expression vector pGADT7 (Clontech ...
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bioRxiv - Plant Biology 2022Quote: ... these plasmids were co-transformed into the Y2H GOLD strain in an activation domain (AD)-BD coupled manner (Yeast Protocols Handbook, PT3024-1; Clontech). The primers used are listed in Supplemental Table S1.
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bioRxiv - Plant Biology 2022Quote: ... and 3’ UTR region was cloned into the BamHI/XhoI site of pBluescript KS– using an In-Fusion HD cloning kit (Takara), yielding pOsSOG1_7A ...
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bioRxiv - Plant Biology 2022Quote: ... RT-PCR was performed with PrimeSTAR GXL DNA polymerase (Takara) using gene-specific primers (Table S7) ...
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bioRxiv - Plant Biology 2022Quote: ... in-fusion cloning methods (Clontech/Takara) were employed to PCR-amplify and clone from S.viridis A10 genomic DNA a 4 kb fragment containing the SvRA1 transcribed region and regulatory sequences into pSB11-BAR as a HindIII-BamHI insertion ...
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bioRxiv - Plant Biology 2022Quote: ... The HMWG::TaVIT2-D sequence was inserted by In-Fusion cloning (Takara Bio) in the HindIII restriction site upstream of ZmUBI1::OsNAS2 ...