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Citations for Takara Bio :
6301 - 6350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... pGFP-N2 expression vector was obtained from Clontech.
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bioRxiv - Molecular Biology 2023Quote: ... Lenti-XTM 293T cells (632180, TAKARA, JP) were maintained in DMEM supplemented with fetal bovine serum (10% ...
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bioRxiv - Molecular Biology 2023Quote: ... All the DNA templates were prepared by PCR (Takara) unless otherwise noted ...
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bioRxiv - Microbiology 2023Quote: ... The PCR reaction mixture (100 nL) was prepared using SmartChip TB Green Gene Expression Master Mix (TaKaRa Bio), nuclease-free PCR-grade water ...
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bioRxiv - Microbiology 2023Quote: ... DNA extracts were sent to Resistomap Oy (Helsinki, Finland) for HT-qPCR analysis using a SmartChip Real-Time PCR system (TaKaRa Bio, Shiga, Japan). 10 ng DNA of each of the extracted samples were shipped to Resistomap for analysis ...
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bioRxiv - Molecular Biology 2023Quote: ... It was amplified using PrimerSTAR HS DNA polymerase (Takara), digested with restriction enzymes ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with an additional 1 mM ATP (TAKARA Bio, 4041), at room temperature for 1 hour ...
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bioRxiv - Microbiology 2023Quote: Lenti-X 293T (TaKaRa, Cat# Z2180N), PK-15 (Japanese Collection of Research Bioresources Cell Bank (JCRB) ...
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bioRxiv - Microbiology 2023Quote: ... Synthesized DNA was cloned into the pDON-5 Neo-vector (TaKaRa, Kusatsu, Japan, Cat# 3657), which was prelinearized with NotI-HF (New England Biolabs [NEB] ...
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bioRxiv - Microbiology 2023Quote: ... and pMD2.G plasmid with TransIT-293 Transfection Reagent (TaKaRa, Cat# V2700) in Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... total RNA was collected using a CellAmp Direct RNA Prep Kit for RT-PCR (Real Time) (TaKaRa, Cat# 3732). GAPDH and vRNA levels were measured with a RT-qPCR assay using the One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time) ...
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bioRxiv - Microbiology 2023Quote: ... using an In-Fusion HD Cloning Kit (TaKaRa, Cat# Z9633N). Plasmids were amplified using NEB 5-alpha F′ Iq competent Escherichia coli (NEB ...
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bioRxiv - Microbiology 2023Quote: ... pGP packaging plasmid (TaKaRa, Cat# 6160), and pMD2.G plasmid with TransIT-293 Transfection Reagent (TaKaRa ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was then pre- hybridized in 10 mL ExpressHyb (Clontech, NC9747391) at 44°C in a rotating oven for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... A quantitative (q)PCR was performed on a Thermal Cycler Dice Real Time System (Takara) with the use of Power SYBR Green Master Mix (A25742 ...
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bioRxiv - Microbiology 2023Quote: Y2H assays were performed according to Yeastmaker™ Yeast Transformation System 2 User Manual (Clontech). The coding sequences corresponding to HCPro1 ...
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bioRxiv - Microbiology 2023Quote: ... The viral RNA copy number was standardized using a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). Fluorescent signals from resulting PCR products were acquired using a Thermal Cycler Dice Real Time System III (Takara).
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bioRxiv - Molecular Biology 2023Quote: ... SsoFast EvaGreen Supermixe (Takara) was used along with 5 pmol each of forward and reverse primer and 50ng of DNA for the PCR.
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bioRxiv - Plant Biology 2023Quote: ... and genomic DNA was removed by TaKara DNase I ...
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bioRxiv - Synthetic Biology 2023Quote: Plasmid cloning was performed using In-Fusion cloning (Takara Bio) for large rearrangements and Q5® Site-Directed Mutagenesis Kit (New England BioLabs ...
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bioRxiv - Cell Biology 2023Quote: ... The DNA fragment coding GFP was inserted at the 5’ side of SYP32 by In-Fusion HD Cloning Kit (Takara), and the whole sequence was recombined into pGWB1 by LR Clonase II ...
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bioRxiv - Plant Biology 2023Quote: ... α-galactosidase units were calculated according to the protocol (Clontech, P3024-1).
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The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregationbioRxiv - Cell Biology 2023Quote: ... transfection was performed using Xfect Transfection Reagent (Takara Bio, 631317) with 5 μg of DNA following manufacturer’s instructions and cells were imaged 24h post-transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... A homology-directed repair template donor vector where frt-PGK-Hygro-pA-frt-T2A-mScarlet-Akaluc-T2A-CreER-P2A-DTR is flanked by the homology arms was cloned into the pUC19 plasmid backbone (#638949, Takara). Donor vector and RNP complex were transfected into mESC line harboring Krasfrt-stop-frt(LSL)-G12D/+ ...
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bioRxiv - Plant Biology 2023Quote: ... 25 mM ATP and 1.0 U μL-1 RNase inhibitor (Takara Bio) at 22°C for 1 to 2 h ...
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bioRxiv - Plant Biology 2023Quote: ... and end-labeled by T4 polynucleotide kinase (Takara Bio) and [γ-32P]-ATP (PerkinElmer) ...
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bioRxiv - Plant Biology 2023Quote: ... and then probes for siRNA detection were made using the BcaBEST Labeling Kit (Takara Bio) with [α-32P]dCTP (PerkinElmer ...
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bioRxiv - Plant Biology 2023Quote: ... 32P-labeled dsRNAs were synthesized using the in vitro T7 Transcription kit (Takara Bio) with [α-32P]-UTP (PerkinElmer ...
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bioRxiv - Plant Biology 2023Quote: ... according to the manufacturer’s instructions and treated with DNase I (Takara) to remove genomic DNA ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitative real-time PCR using SYBR Green based method (DSS TAKARA) was employed to estimate relative transcript levels of mRNAs with GAPDH as housekeeping control gene for internal normalization ...
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bioRxiv - Plant Biology 2023Quote: ... pYC96 (pGADT7-AGG2) and pYC97 (pGADT7-AGG3) for expression in Saccharomyces cerevisiae strain Y2HGold or Y187 (Takara Bio).
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bioRxiv - Plant Biology 2023Quote: ... and AGGs into the pGADT7 AD vector (Takara Bio) to produce pYC59 (pGBKT7-AGB1.1) ...
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bioRxiv - Synthetic Biology 2023Quote: ... lentiviral particles were harvested and concentrated using Lenti-X concentrator (Takara Bio) and stored at -80°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... the PrimeSTAR Max DNA Polymerase (Takara Bio) or Platinum Superfi II DNA polymerase (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... On Day 2 virus coated culture plates were prepared on non TC-treated 12-well plates that had been pre-coated with RetroNectin (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... and was reverse transcribed using a PrimeScript RT Reagent Kit (Takara, Beijing, China). qPCR was performed using TB Green Premix Ex Taq (Takara ...
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bioRxiv - Synthetic Biology 2023Quote: ... and the PpERV2 cassette (PPDC1-PpERV2-Tsynth19) were ligated in tandem with pMD18-T (Takara, Beijing, China) to construct LHZ1016 ...
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bioRxiv - Molecular Biology 2023Quote: Lenti-X HEK293T cells (Takara Bio) were cultured in DMEM (1X ...
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bioRxiv - Evolutionary Biology 2023Quote: Complete cDNA sequences of taste receptors were obtained using a SMARTer 5’/3’ kit for RACE PCR to obtain 5’ sequence information missing from reference transcriptome (Takara, #634858). Briefly ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was synthesized from the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa) for RT-qPCR.
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bioRxiv - Plant Biology 2023Quote: ... Primer Star high fidelity DNA polymerase and restriction enzymes were purchased from TaKaRa (Beijing). Ordinary Taq enzyme was purchased from Tiangen (Beijing).
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bioRxiv - Plant Biology 2023Quote: Then 3 uL of MNase (Takara) was added to each sample and the incubation lasted for 15min at 37 °C ...
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bioRxiv - Genomics 2023Quote: ... and concentrated by Lenti-X concentrator (Takara Bio, 631232) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... mouse anti-GFP (Clontech,1:6,000), mouse anti-FLAG (Millipore-Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... by PCR using a PrimeSTAR Mutagenesis Basal Kit (Takara, R046A) and the following primers ...
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bioRxiv - Genetics 2023Quote: ... The media are from Clontech Laboratories ...
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bioRxiv - Genetics 2023Quote: ... and selection was performed on SD-Trp-Leu plates containing 200 ng/ml of Aureobasidin A (Clontech, 630466) according to the manufacturer’s instructions (Clontech) ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by PCR amplification using Ex-Taq polymerase (Takara) and template cDNA ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA libraries were prepared using the PrepX RNA-seq directional protocol (Takara Bio) following the manufacturer’s instructions and utilizing an Apollo 324 robot ...
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bioRxiv - Developmental Biology 2023Quote: ... and used for sex determination according to the manufacturer’s instruction of Ex Taq Hot Start Version (Takara Bio, Shiga, Japan). The amplification was conducted as follows ...