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Citations for Takara Bio :
6251 - 6300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The cDNA sequences of CHIKV 6K and TF (Supplementary Figure S1) were cloned in the pEGFPC1 vector (Clontech) to generate fusion proteins tagged with the Enhanced Green Fluorescent Protein (EGFP ...
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bioRxiv - Cell Biology 2023Quote: The pik1-11 temperature sensitive allele was constructed as described (Tang et al., 2019) with the exception that EX taq polymerase (Takara, cat# 4025) and accompanying dNTPs (Takara ...
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bioRxiv - Cell Biology 2023Quote: ... and accompanying dNTPs (Takara, cat# RR01BM) were used.
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bioRxiv - Cell Biology 2023Quote: ... The reporter cassette excised from pPExRF3 and the left and right homology arms were assembled and cloned into a linearized pUC19 in a single enzymatic reaction using the In-Fusion Cloning Kit (Clontech Laboratories Inc., California, U.S.A). A gRNA vector was constructed by cloning a 20-bp target sequence surrounding the stop codon of coronin into pU6 (FlyCRISPR) ...
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bioRxiv - Neuroscience 2023Quote: ... We obtained 20 ng/ul RNAs (total 10 ul eluted RNA from one microdissected cell body) that we used for RNAseq (Clontech SMART-Seq Ultra Low Input RNA kit) in Scripps Florida Genomics Core (Currently known as The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology) ...
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bioRxiv - Neuroscience 2023Quote: ... Cold Lenti-X Concentrator (Takara; Cat. No. 631232) corresponding to one-third volume of the supernatant was added to the filtered solution ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was obtained by reverse transcription using the PrimeScript RT Reagent Kit (Takara, Kusatsu, Japan). RT-qPCR was performed with the respective human-specific sense and antisense primers and RT-SYBR™ Green PCR Master Mix (Applied Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: ... transfected iPSCs were selected for 48 hours with 0.5 μg/mL puromycin (Takara). Differentiation of iPSCs into iNeurons was performed using an established protocol35 ...
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bioRxiv - Cell Biology 2023Quote: ... and of human FAM104B isoform 3 (NM_001166700.2) were PCR- amplified from a yeast two-hybrid human testis cDNA library (Clontech, cat. no. 638810) and cloned via appropriate restriction sites into pGAD-C1 (James et al ...
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bioRxiv - Cell Biology 2023Quote: ... by replacing EGFP-coding sequences with mCherry-coding sequences using In-Fusion® HD Cloning Kit (Clontech).
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bioRxiv - Cell Biology 2023Quote: ... harvested in Trizol (Takara), and stored at -80 °C ...
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Tight junction membrane proteins regulate the mechanical resistance of the apical junctional complexbioRxiv - Cell Biology 2023Quote: ... using an In-Fusion HD Cloning Kit (Takara; #Z9649N). pCANw-HA-cZO1-Flag was created by inserting an HA tag into the N-terminus of cZO-1 by PCR using the following primers ...
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bioRxiv - Neuroscience 2023Quote: ... or TB Green Premix Ex Taq II (Tli RnaseH Plus) Kit (Takara), 1μM primers and 1μL of cDNA ...
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bioRxiv - Neuroscience 2023Quote: ... or the PrimeScript RT Master Mix (Takara). In each individual experiment ...
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bioRxiv - Cell Biology 2023Quote: ... and 5 µg RNA was reverse transcribed into cDNA using a cDNA synthesis kit (Takara Bio) as per the manufacturer’s instructions ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... The pLVSIN-EF1α-AcGFP-C1 vector (5.5 μg) was added to 7 μL Lentiviral Mix High Titer Packaging Mix (Takara Bio Inc., Otsu, Japan), 1500 μL serum-free DMEM ...
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bioRxiv - Neuroscience 2023Quote: ... 1.25 x105 cells/cm2 were immediately added to each well with HEK293 maintenance media modified with 10% tetracycline-free FBS (Takara Bio, Cat. No. 631367). After 24 h ...
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bioRxiv - Molecular Biology 2023Quote: ... flanking the site of alterations (HDE-like motifs) were amplified from the genomic DNA using CloneAmp HiFi PCR Premix (Clontech), A-tailed with DreamTaq DNA polymerase and ligated into pGEM-T Easy vector ...
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Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: ... mtDNA was then amplified by PCR using PrimeSTAR® GXL polymerase (Takara). PCR was performed using two sets of slightly overlapping primers to reduce primer bias ...
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bioRxiv - Microbiology 2023Quote: ... anti-GAPDH antibody (Clontech) at a 1/2500 dilution ...
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bioRxiv - Immunology 2023Quote: ... cDNA libraries were constructed using the SMARTer Stranded Total RNA-Seq Kit (v2) – Pico Input Mammalian (Clontech) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Clarified supernatant was incubated with His60 NTA resin (TAKARA Bio) for 1 hour then loaded onto gravity flow columns and eluted with Buffer A containing 200 mM Imidazole ...
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bioRxiv - Molecular Biology 2023Quote: ... A mitochondrial DNA monitoring primer set kit from Takara Bio [San Jose ...
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bioRxiv - Molecular Biology 2023Quote: ... For quantitative RT-PCR 1ug of total RNA was treated with 5 Units of DNase I (Takara) and cDNA synthesized with SuperScriptIII (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... STM ΔspeED by RNA isolation was performed using TRIzol (Takara) reagent according to manufactures’ protocol RNA was quantified using Thermo-fischer scientific Nano Drop followed by running on 2% agarose gel for checking the quality ...
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bioRxiv - Microbiology 2023Quote: ... QuickCutTM Hind III (TAKARA, 1615), TwistAmp® Basic Kit (TwistDx ...
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bioRxiv - Microbiology 2023Quote: ... The fragments were joined using In-Fusion (TaKaRa), producing plasmid pTV002 ...
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bioRxiv - Microbiology 2023Quote: ... and the RNA was extracted immediately using a bacterial RNA extraction kit (TaKaRa). The purified RNA extracts were reverse-transcribed to cDNA ...
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bioRxiv - Neuroscience 2023Quote: ... the SV40pA was replaced by the 3’UTR of msSOD2 (Kaltimbacher et al., 2006) amplified from mouse brain cDNA (Clontech 637301) using primers AA35 and AA36 and inserted into the XbaI and NotI sites ...
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bioRxiv - Molecular Biology 2023Quote: ... the total RNA of three samples in each group was extracted with the TaKaRa MiniBEST Universal RNA Extraction Kit (TaKaRa, Japan), and linear RNA was then removed with Rnase R (GENESEED ...
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bioRxiv - Molecular Biology 2023Quote: ... The TaKaRa MiniBEST Universal RNA Extraction Kit (TaKaRa, Japan) was used to extract the total RNA from the total RNA of larval gut samples in the AcCK1 ...
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bioRxiv - Neuroscience 2023Quote: Fhod3 cDNA was amplified with PrimeSTAR® GXL DNA Polymerase (R050B; Takara Bio, San Jose, CA, USA) using a pair of primers (5’-CACCATGGCCACGCTGGCTTGTCGCGTGCAG3’ for the forward and 5’-GAGCATGCTCACAGTTGCAGTTCAGATGTG −3’ for the reverse primer) ...
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bioRxiv - Genetics 2023Quote: ... Reverse transcription was performed using a SMARTer Ultra Low RNA Kit (Clontech, USA) directly in the cell lysate ...
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bioRxiv - Physiology 2023Quote: ... in the in pLVX-IRES (for ALDOA; #631849, Takara) for doxycycline-inducible expression in mammalian cells ...
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bioRxiv - Physiology 2023Quote: ... was validated in vitro (sgRNA screening system, Clontech), and obtained from PNAbio ...
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bioRxiv - Physiology 2023Quote: ... 1 μg of total RNA was reverse transcribed using RNA to cDNA EcoDry™ Premix (Double Primed) (Takara Bio USA Inc., San Jose, CA, USA) according to the manufacturer instruction ...
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bioRxiv - Neuroscience 2023Quote: Cultured neurons were transfected using the CalPhos Mammalian Transfection Kit (Takara Cat# 631312). Briefly ...
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bioRxiv - Plant Biology 2023Quote: ... Fragments amplified with primers for CAPS markers were digested with appropriate restriction enzymes supplied by Takara Bio (Kusatsu ...
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bioRxiv - Plant Biology 2023Quote: ... the genomic sequence from the MpRKD promoter region (3827 bp upstream from start codon) to the 3’UTR was amplified by PCR with PrimeSTAR HS DNA polymerase (TaKaRa Bio, Kusatsu, Japan) and the primers IF-Hind-pRKD-F and IF-Sal-gRKD-R ...
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bioRxiv - Neuroscience 2023Quote: ... Co-purified cellular RNAs were degraded by incubating with 100U micrococcal nuclease (Clontech, 2910A) on-resin in 25 mL of Nickel wash buffer supplemented with 5 mM CaCl2 at room temperature for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... fixed for 1 h in 4% paraformaldehyde/PBS (TAKARA BIO INC. Cat#T900), and subjected to antibody labeling ...
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bioRxiv - Plant Biology 2023Quote: ... selections and LacZ reporter assays were made according to the Matchmaker Gold Yeast Two-Hybrid System user manual (Clontech; Mountain View, Ca, USA).
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bioRxiv - Plant Biology 2023Quote: ... was performed to determine the expression level of transcripts utilizing the SYBR Green PCR master mix kit (Takara). The unique region of transcripts was selected for designing qRT-PCR primers and mentioned in Table S3 ...
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bioRxiv - Plant Biology 2023Quote: ... and cloned into the pSL vector containing the Nicotiana tabacum PR1 signal peptide using the In-fusion cloning kit (Takara Bio USA Inc., San Jose, USA) to allow efficient secretion in N ...
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bioRxiv - Physiology 2023Quote: ... Full-length cDNA was generated using the TaKaRa SMART-Seq v4 Ultra Low kit (TaKaRa Cat # 634889). mRNA-Seq libraries were then prepared using the Illumina Nextera XT DNA Library Preparation kit (Illumina Cat # FC-131-1024) ...
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bioRxiv - Physiology 2023Quote: ... ReverTra Ace qPCR RT Master Mix with gDNA Remover (cat. FSQ-301) was purchased from Toyobo. PrimeSTAR HS polymerase (cat. R40A) was purchased from Takara. Dry yeast (cat ...
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bioRxiv - Microbiology 2023Quote: ... The resulting plasmid was transformed into Stellar competent cells (Clontech), isolated ...
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bioRxiv - Microbiology 2023Quote: ... Site-directed mutagenesis was performed using PrimeSTAR Max DNA polymerase (Takara Bio) with the following settings ...
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bioRxiv - Microbiology 2023Quote: ... Total RNAS were extracted from these sediments using TRIzol® Reagent according the manufacturer’s instructions and genomic DNA was removed using DNase I (TaKara, Japan). Then RNA quality was determined using 2100 Bioanalyser (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: ... These bulk kidney tissues were process from an OCT block using the SMARTer Stranded Total RNA-Seq Kit v2 (Takara). Sequencing was performed in a 2×75 bp paired-end configuration using a NovaSeq platform (Illumina).