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Citations for Takara Bio :
6201 - 6250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Expression plasmids for other His-mCherry or His-GFP fusion proteins were constructed using in-Fusion HD Cloning Kit (Takara Bio, Inc.). All mCherry/GFP fusion proteins were expressed in E ...
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bioRxiv - Biochemistry 2023Quote: The plasmids of TiCGSCy mutants (E1442Q, E1442A and E1356A) were constructed using a PrimeSTAR mutagenesis basal kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... A gene region encoding TiCGSCy was amplified by PCR using PrimeSTAR Max DNA Polymerase (Takara Bio, Shiga, Japan) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... The amplified product digested with NdeI and XhoI (Takara Bio) was ligated into the pET30a vector (Merck ...
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bioRxiv - Molecular Biology 2023Quote: ... The SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian (Takara) together with a NovaSeq platform (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: For indexing PCR, 78μL PCR mix (24μL H20, 50μL 2X SeqAmp CB PCR buffer (Takara Cat. #638526), 2μL SeqAmp DNA polymerase ((Takara Cat ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by gel purification (Takara Bio Cat. #740609.50). Samples were then pooled and sequenced on a lane of HiSeq 4000 SE50 at the UCSF Center for Advanced Technology (CAT).
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bioRxiv - Cancer Biology 2023Quote: ... 2μL SeqAmp DNA polymerase ((Takara Cat. #638509), 2μL 10uM universal reverse primer (seq ...
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bioRxiv - Cell Biology 2023Quote: A cDNA fragment encoding human FAM53C was isolated by amplifying with nested PCR from human cDNA library plasmid (Takara). The oligonucleotide primer sequences used for the 1st PCR are 5′-CAAAGTGTGCAAGTCAAATCCTGG-3′ (5′ upstream ...
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bioRxiv - Immunology 2023Quote: ... cDNA library was generated from 2 nanograms of total RNA using Smart-Seq V4 Ultra Low Input RNA Kit (Takara catalog#: 634894). 150 picograms of cDNA was used to make sequencing libraries by Nextera XT DNA Sample Preparation Kit (Illumina catalog# ...
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bioRxiv - Microbiology 2023Quote: ... ligated using T4 ligase (Takara) and transformed into chemically competent E ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from cells and reverse-transcribed to cDNA using the PrimeScript 1st Strand cDNA Synthesis Kit (TaKaRa Bio). cDNA was used as a template for real-time qPCR analysis on CFX96 Touch (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... The virus particles were further concentrated at 10X in volume using Lenti-X-concentrator (Clontech, PT4421-2) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: Broccoli RNA template sequences were ordered as dsDNA gBlock as above and inserted into a linearized pRZ plasmid using infusion cloning kit (Takara Bio) and transformed into Stellar cells following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The mutant 2X FLAG TANGO2 Gly154Arg was generated with the In-Fusion HD Cloning Plus Kit (Takara Bio Europe) following the manufacturer’s instructions using the pMK-T plasmid as template ...
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bioRxiv - Neuroscience 2023Quote: ... Adeno-associated virus (AAV) cell-lysates were produced using the AAVpro Purification Kit (All Serotypes) (Takara) with slight modifications ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were prepared from these samples using the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara) kit according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... to 7.5μL eluted RNA 2.5μL smRNA mix 1 (Takara Cat. #635031) and 1μL 10uM UMI RT primer (seq ...
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bioRxiv - Bioengineering 2023Quote: ... cDNA was generated from each sample employing the Prime Script RT Master Mix (Perfect Real Time) (Takara Bio Inc., Japan). Quantitative RT-PCR was performed using TB Green Premix Ex Taq II (Takara Bio Inc. ...
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bioRxiv - Biophysics 2023Quote: ... The first-strand cDNA was reverse transcribed with PrimeScript™ RT Reagent Kit with gDNA Eraser (TaKaRa, Japan). Quantitative real-time PCR was run on a Roche LightCycler® 480 II real-time PCR detection system ...
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bioRxiv - Biophysics 2023Quote: ... VLP-containing supernatant was sterile filtered through a 0.45 µm PES filter and fiftyfold enriched using LentiX concentrator following the manufacturer’s protocol (Takara). The VLPs were diluted 1:1 with PBS and stained at a final concentration of 300 nM Flipper or 100 nM NR12S or NR12A before imaging in μ-Slides (18 well glass bottom ...
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bioRxiv - Biophysics 2023Quote: ... The virus of each construct was produced in Lenti-X™ 293 T Cell Line (Takara, Cat. #632180) and previously described74 ...
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bioRxiv - Biophysics 2023Quote: ... HaloTag was replaced with sfGFP using InFusion (Takara) assembly by cutting out the HaloTag using FD-PacI (Thermo ...
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bioRxiv - Biophysics 2023Quote: Human cell lines used in this study include U2OS and Lenti-X 293T (Takara Bio USA). Lenti-X 293T cells were only used for virus production ...
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bioRxiv - Biophysics 2023Quote: Plasmids were transformed into Stellar cells (Clontech), from which single colonies were picked ...
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bioRxiv - Biophysics 2023Quote: ... We concentrated the supernatant with Lenti-X concentrator (Takara) following the manufacturer’s protocol and resuspend it in RPMI ...
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bioRxiv - Cancer Biology 2023Quote: ... Virus-containing supernatant was harvested at 48 and 72 hours after transfection and viral particles were concentrated with Lenti-X Concentrator according to the manufacturer’s instructions (Takara Bio cat#631232). Lenti-X GoStix Plus were then used to determine virus titer (Takara Bio cat#631280) ...
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bioRxiv - Bioengineering 2023Quote: Single colonies were grown in 10 ml YPDA media (Takara) in 50 ml tubes for 24h ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-dsRed (Clontech, 632496, 1:250), mouse anti-HuC/D (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was prepared using the PrimeScript II 1st Strand cDNA Synthesis Kit (TaKaRa Bio). The first targeted PCR was performed using a KAPA HiFi HotStart ReadyMix PCR Kit (Roche ...
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bioRxiv - Microbiology 2023Quote: ... Amplification was performed using the Tks Gflex DNA Polymerase Low DNA (Takara Bio). Thermal cycles were as follows ...
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bioRxiv - Microbiology 2023Quote: ... qPCR was performed using the TB Green Fast qPCR Mix (Takara Bio) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Real-time RT-PCR was performed using SYBR green (Takara) in QuantStudio3 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Biochemistry 2023Quote: ... Uncleaved protein and cleaved His6-Ztag domains were separated from cleaved protein by incubation with TALON resin (Clontech). The supernatant was buffer exchanged to MES A buffer (20 mM MES ...
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bioRxiv - Microbiology 2023Quote: ... Amplified fragments were purified using 0.55x beads/sample ratio NucleoMag® NGS magnetic beads (Takara Bio). Amplicons were prepared for Oxford Nanopore sequencing using the ligation sequencing kit SQK-LSK110 (Oxford Nanopore Technologies ...
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bioRxiv - Immunology 2023Quote: ... This RNA was used as input to the Mir-X™ miRNA qRT-PCR TB Green® Kit (Takara cat# 638316) to generate miRNA cDNA ...
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bioRxiv - Neuroscience 2023Quote: ... and rabbit anti-dsRed2 (1:1000, Clontech). The following secondary antibodies were used ...
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bioRxiv - Cell Biology 2023Quote: ... The coding sequence of EPHX2 was amplified by PCR from liver cDNA (Human Multiple Tissue cDNA panels; Takara Bio, Shiga, Japan) using primers (5’-GTCGACATGACGCTGCGCGCGGCCGTCTTCG-3’ ...
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bioRxiv - Genomics 2023Quote: ... The first-strand cDNA was amplified with PrimeSTAR GL DNA polymerase (Clontech) to generate double-stranded cDNA ...
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bioRxiv - Cell Biology 2023Quote: ... The copy number was calculated using the standard curves for cloned Tert and Dloop in a pMD19-T Easy vector (TaKaRa, 3271), respectively ...
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bioRxiv - Cell Biology 2023Quote: ... The upstream and downstream regions adjacent to the binding site were amplified using Advantage 2 polymerase (Takara Bio, Shiga, Japan) from 3T3-L1 genomic DNA and cloned into the HR110-PA-1 vector (System Biosciences) ...
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bioRxiv - Cell Biology 2023Quote: ... Yeast 2-hybrid (Y2H) analysis was performed using the protocols described in the Yeast protocols handbook (Clontech, Protocol No ...
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bioRxiv - Cell Biology 2023Quote: ... Extracted gDNA was PCR amplified (Takara, RR001A) using a 30-cycle protocol ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... cDNA of NRASQ61R was cloned into the pLVSIN-EF1α-AcGFP-C1 vector (Takara Bio Inc., Otsu, Japan).
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: Using the Lenti-X 293 T cell line (Takara Bio Inc., Otsu, Japan) and the pLVSIN-EF1α-AcGFP-C1 vector ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... and 45 μL Trans IT-293 Transfection Reagent (Takara Bio Inc., Otsu, Japan). After 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... Shield1 (632189) (TAKARA); colcemid (15212-012 ...
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bioRxiv - Cell Biology 2023Quote: ... Extracted gDNA was PCR amplified (Takara, RR001A) using a 30-cycle protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... were packaged in Lenti-X 293T Cells (Takara Bio, Cat. 632180) by transient transfection ...
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bioRxiv - Cell Biology 2023Quote: ... into pEGFP-N1 vector (Clontech). All PCRs were performed using Phusion High-Fidelity DNA Polymerase (New England Biolabs).