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Citations for Takara Bio :
6151 - 6200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... triple transfection of HEK-293T cells was performed using the pHelper plasmid (TaKaRa, USA), trans-plasmid with either the AAV2/9 ...
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bioRxiv - Biochemistry 2023Quote: ... Genomic DNA from WT and Strt1−/− cells was extracted with a NucleoSpin Tissue kit (Takara Bio, Shiga, Japan) and subjected to PCR using specific primers (Oligonucleotide sets 3 ...
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bioRxiv - Bioengineering 2023Quote: ... The synthesized mRNA was subsequently purified using a NucleoSpin® RNA Clean-Up Kit (Takara Bio Inc., Japan). The concentration of the purified mRNA was quantified using a Nanodrop One (Thermo Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... the input binary libraries (1:10 or 1:100) and the selected pools were subjected to restriction digestion using BamHI (Takara Bio Inc., Japan). The digestion products were then analyzed using a 10% polyacrylamide gel electrophoresis (PAGE ...
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bioRxiv - Cancer Biology 2023Quote: ... Lenti-X 293T cells (Takara, 632180) and primary human lung fibroblasts (Lonza Bioscience ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 10% FBS (Takara Bio, 631367), at 37 °C in 0.5% CO2 ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with TALON resin (Takara Biotech, Saint-Germain-en-Laye, France) for 90 min in a ratio of 1 ml resin per 10 g of cells ...
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bioRxiv - Bioengineering 2023Quote: ... 3µg of DNA was digested by EcoRI and the digested DNA was self-ligated using DNA Ligation Kit (Mighty Mix; Takara Bio, Kusatsu, Japan) after purification by ethanol precipitation ...
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bioRxiv - Biochemistry 2023Quote: ... Cloning was performed with In-Fusion cloning kit (Clontech). The list of primers is available in the Supplementary Table S1.
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bioRxiv - Biophysics 2023Quote: ... Viral vector titers were determined by the number of DNase I resistant vg using AAV titration kit (TaKaRa/Clontech) by qPCR (StepOne ...
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bioRxiv - Biophysics 2023Quote: ... Viral vector titers were determined by the number of DNase I resistant vg using AAV titration kit (TaKaRa/Clontech) by qPCR (StepOne ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted using NucleoSpin RNA Plus XS (740990.250; Takara Bio) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Genomic DNA was extracted using a NucleoSpin Blood XL kit (740950.50; Takara Bio) and further cleaned up by a OneStep PCR Inhibitor Removal Kit (D6030 ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA sequences of PAK1 mutants with a C-terminus Flag tag were synthesized at BGI Genomics (Beijing, China) then cloned into pLVX-IRES-Neo vector (632184; Takara Bio, Kusatsu, Shiga, Japan). All sequences are in Extended Data Table 2.
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bioRxiv - Bioengineering 2023Quote: Total RNA was isolated from microbial cells using NucleoSpin RNA (Takara Bio, Otsu, Japan). To prepare a complementary DNA (cDNA ...
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bioRxiv - Bioengineering 2023Quote: ... Quantitative PCR was performed with the SYBR Premix Ex TaqTM II (Takara) with the CFX96 Real-Time PCR System (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... We purified PCR products by agarose gel extraction (Takara Bio, 740609) and used BP clonase (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... which was measured using a real-time PCR assay with a SARS-CoV-2 direct detection RT‒qPCR kit (Takara Bio, Siga, Japan). The IC50 was calculated by IC50 Calculator (https://www.aatbio.com/tools/ic50-calculator ...
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bioRxiv - Immunology 2023Quote: ... The human J chain was replaced with the DsRed2 gene of pDsRedN1 (Takara Bio, Shiga, Japan). The pIgR gene was inserted into the pEF-Myc-His vector (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... S-IgA was purified by two-step chromatography using the Capturem His-Taged Purification Kit (Takara Bio, Shiga, Japan) followed by size exclusion chromatography (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentrations were estimated using a BCA Protein Assay Kit (Takara). For FRAP experiments ...
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bioRxiv - Biochemistry 2023Quote: ... The In-Fusion Snap Assembly (Takara Bio) was used for cloning ...
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bioRxiv - Biochemistry 2023Quote: ... SYBR Green real-time PCR Master Mix was purchased from Takara (Beijing, China). Other antibodies included anti-LDLR (ET1606 ...
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bioRxiv - Cell Biology 2023Quote: ... and were reverse transcribed to first-strand cDNAs with random primers using PrimeScript First Strand cDNA Synthesis Kit (Takara, Japan). Quantitative PCR (qPCR ...
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bioRxiv - Cancer Biology 2023Quote: ... Dox-inducible vectors were generated according to a publicly available protocol54 using In-Fusion HD Cloning Kit (Clontech). Vectors were amplified in Stellar Competent Cells (Clontech ...
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bioRxiv - Cancer Biology 2023Quote: ... 150,000 cells were seeded in 6-well plates 1 day before transfection with 1 μg pp53-TA-Luc vector (Clontech) and 0.015 μg pRL-CMV-Renilla (Promega ...
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bioRxiv - Biophysics 2023Quote: ... 5′-Cy5 and 3′-Cy3 labeled ITS2 RNA was synthesized from Takara Biomedical Technology ...
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bioRxiv - Cancer Biology 2023Quote: ... The antibody used was rabbit polyclonal DsRed (632496; TaKaRa, 1:500).
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bioRxiv - Cell Biology 2023Quote: ... and TB Green Premix Ex Taq II (TaKaRa Bio). In each sample ...
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bioRxiv - Microbiology 2023Quote: ... were used to clone into pET32 vector using In-fusion cloning mechanism (Takara Bio). The FapA construct without the signal sequence (residues 29-152 ...
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bioRxiv - Cancer Biology 2023Quote: ... Vectors were amplified in Stellar Competent Cells (Clontech) and respective integrated shRNA was verified by Sanger sequencing ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR quantitation of mRNAs was performed using TB Green™ Premix Ex Taq™ II (TaKaRa) with Applied Biosystems StepOnePlus Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was isolated from vehicle and Lantana camara leaf ethanolic extract-treated MDA-MB-231 cells using RNAiso Plus (Takara, Japan) as per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Real-time PCR was carried out using a TB Green Premix Ex Taq (Tli RNaseH Plus) kit (Takara, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... cells with Lenti-X Packaging Single Shot (Takara Bio) with one of the following plasmids pLVX-Tet3G ...
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bioRxiv - Cancer Biology 2023Quote: These RNA samples were used for cDNA synthesis using PrimeScript 1st strand cDNA synthesis kit (Takara, Japan) as per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA from oocytes was extracted using MiniBEST Universal RNA Extraction Kit (TaKaRa, Dalian, China), and reverse transcribed to synthesize cDNA using a PrimeScript RT Master Mix reverse transcription kit (TaKaRa ...
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bioRxiv - Cancer Biology 2023Quote: Lentiviral particles were produced in Lenti-X 293T cells (Takara). Approximately 10x106 cells were transfected with a mixture of pVSV-G (1.7 µg) ...
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bioRxiv - Developmental Biology 2023Quote: ... and reverse transcribed to synthesize cDNA using a PrimeScript RT Master Mix reverse transcription kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The number of virus copies/ml was determined using the Lenti-X™ qRT-PCR Titration Kit (Takara Bioscience, 631235) according to the manual ...
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bioRxiv - Genetics 2023Quote: ... Reverse transcription was performed using the oligo (dT) primer and AMV reverse transcriptase contained in the TaKaRa RNA PCR kit (TaKaRa). The RT-PCR was performed using KOD One PCR Master Mix (TOYOBO ...
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bioRxiv - Plant Biology 2023Quote: ... and purified with Capturem™ His-Tagged Purification Maxiprep Kit (Takara). The binding reaction was performed in 20 μL binding buffer (10 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... using SYBR® Premix Ex Taq™ II (TaKaRa). Specific primers for RPL13 ...
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bioRxiv - Cancer Biology 2023Quote: ... Growth media was changed the following day and lentiviruses-containing supernatants were harvested at 72 hr after transfection and concentrated 100-fold using Lenti-X concentrator (Clontech). For infection ...
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bioRxiv - Biochemistry 2023Quote: ... and hSTEAP2 was purified from the supernatant using cobalt-based Talon affinity resin (Clontech, Mountain View, CA). The C-terminal His6-tag was cleaved with tobacco etch virus (TEV ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 2019) upstream of an egfp coding sequence and an SV40 polyadenylation sequence using In-Fusion Snap Assembly master mixes (Takara). To prepare the injection mixtures ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from Col-0 and mutant seedlings using an RNAiso Plus kit (Takara). Complementary DNAs (cDNA ...
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bioRxiv - Plant Biology 2023Quote: ... and the input and pull-down prey proteins were detected by immunoblot using anti-His (M201, Takara, 1:3000 dilution) and anti-GST (G018 ...
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bioRxiv - Genetics 2023Quote: ... the miR-34c locus was ligated into the multiple cloning site (MCS) of the pTetOne plasmid (Takara Bio).
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bioRxiv - Cancer Biology 2023Quote: ... Centrifuged lysate was purified by gravity-flow chromatography on TALON Metal Affinity Resin (Clontech) following the manufacturer’s protocol with 0.05% Triton X-100 added to all buffers ...